Life Sciences Commons^™

Effect Of Chronic Treatment With Diuretics On Mouse Liver: A Morphological And Microanalytical Investigation, Ann-Christin Mork, Anne Von Euler, Godfried M. Roomans

Scanning Microscopy

In an attempt to produce an animal model for the disease cystic fibrosis (CF), mice were treated chronically with the diuretics amiloride and furosemide, in order to cause chronic inhibition of transepithelial ion transport. Experiments were carried out on adult mice (2 months treatment); in addition, pregnant mice were treated with diuretics, and tissue from offspring 2 and 7 days post partum was investigated. Since biliary cirrhosis is a common occurrence in CF, hepatocytes in the treated mice were investigated by X-ray microanalysis and by light and electron microscopy.

Treatment with amiloride caused a significant decrease in cellular Na concentration …

The Normal, Cryptorchid And Retractile Prepuberal Human Testis: A Comparative Morphometric Ultrastructural Study Of 101 Cases, Saverio Cinti, Giorgio Barbatelli, Claudia Pierleoni, Manlio Caucci

Scanning Microscopy

Fifty-two surgical biopsies from retractile testes of patients in pediatric age (3-14 years), of which 25 were treated with hormonal therapy (RT) and 27 did not undergo therapy before orchidopexy (RNT), were compared with the biopsies of 19 normal (N) and 30 cryptorchid or ectopic (E) testes. A light and electron microscopic morphologic and morphometric study was performed. For the quantitative investigation 4 parameters were selected: a) the mean tubular diameter (on 20 cross-sections); b) the mean spermatogonial number per tubular section; c) the mean nucleolar area of the Sertoli cells; and d) the mean thickness …

Response Of The Esophageal Epithelium To Concomitant Cis-Dichlorodiammineplatinum(Ii) And Radiation Treatment. An Electron Microscopic Study In Rabbits, M. Albertsson, M. Cwikiel, C. H. Håkansson, M. Palmegren

Scanning Microscopy

The rabbit esophageal mucosa was irradiated with daily fractions of 2 Gy up to an accumulated dose of 20 Gy (total dose 2, 6, 10. 16 or 20 Gy). Fifteen to forty-five minutes before the start of each irradiation 0.3 mg Cis-dichlorodiammineplatinum (cis-DDP, cisplatinum) was given by intraperitoneal injection to each rabbit. Examinations were carried out 1-10 days after each fractionation schedule, when specimens were taken for morphological investigations.

Scanning electron microscope (SEM) examination showed a gradual development of damage with cell loss and structural disarrangement of the microridges and whorls on the surface. However, with further treatment the esophageal …

Morphological Study Of Gastric Lesions Developing In The Rat Under Several Damaging Conditions: Modifications Induced By Pretreatment With Zinc Acexamate, M. Luisa Bravo, Ginés Escolar, Carmen Navarro, Ramón Fontarnau, Oriol Bulbena

Scanning Microscopy

Lesions developing in the gastric mucosa of the rat after exposure to different gastric damaging agents (100 mg/kg aspirin, and 70% or 100% ethanol) were assessed by scanning electron microscopy. The severity of the lesions was quantified according to morphological criteria. Modifications in the severity of these lesions induced by pretreatment with zinc acexamate were also analyzed. The scanning electron microscope revealed that with the exception of absolute ethanol, which caused distinctive morphological features, lesions found under the different experimental agents shared a common pattern of progression. Ultrastructural lesions on surface epithelial cells preceded further alterations of parietal cells. After …

Review And New Case Reports On Scanning Electron Microscopy Of Pili Annulati, Monilethrix And Trichothiodystrophy, K. Meyvisch, M. Song, N. Dourov

Scanning Microscopy

Pili annulati, Monilethrix and Trichothiodystrophy are uncommon conditions in which the hair shaft has a distinct appearance as seen by optical microscopy and scanning electron microscopy (SEM). We report several new cases and review the characteristic ultrastructural abnormalities investigated by SEM.

Pili annulati: abnormal areas which show a longitudinal, "curtain-like" folding of the cuticular cells, alternating with normal areas. The latter present regularly non-systematized, superficial depressions. Hair specimens of the patient's mother show the same surface irregularities.

Monilethrix: most hair shafts show variations in thickness giving a typical nodal appearance. In the isthmus area we noticed longitudinal ridging …

A Light, Transmission And Scanning Electron Microscope Study Of Snuff-Treated Hamster Cheek Pouch Epithelium, S. H. Ashrafi, A. Das, R. Worowongvasu, B. Mehdinejad, J. P. Waterhouse

Scanning Microscopy

The effects of smokeless tobacco (snuff) on hamster cheek mucosa were studied by light microscopy, transmission (TEM) and scanning electron microscopy (SEM). Two grams of commercially available smokeless tobacco were placed into the blind end of the right cheek pouch of each experimental animal, once a day and five days a week for 24 months. The control animals did not receive smokeless tobacco. After 24 months treatment with smokeless tobacco, hamster cheek mucosal epithelium lost its translucency and had become whitish in color. By light microscopy hyperorthokeratosis, prominent granular cell layers with increased keratohyalin granules and hyperplasia were seen. At …

Structure Of Margarines Made With Low Erucic Acid Rapeseed Oil, J. Hojerova, S. Schmidt, J. Krempasky

Food Structure

Margarine based on hydrogenated low erucic acid rapeseed oil shows a strong tendency to produce an unacceptable grainy structure. This is caused by rapid transition of the B-form into the B-form of fat crystals. Sorbitan esters, monoacylglycerols, and a blend of acylglycerols were investigated as possible B-stabilizers. Polymorphic transitions were evaluated by visual assessment, examination by polarized light microscopy, and x-ray diffraction analysis in margarines prepared on a laboratory scale and containing varying amounts of stabilizers. The evaluation was extended over a 7-week period of storage at 10 C. Sorbitan esters of fatty acids were found to be the most …

Structure And Rheology Of String Cheese, S. Taneya, T. Izutsu, T. Kimura, T. Shioya

Food Structure

String cheese samples ranging in pH from 5.0 to 5.9 were prepared by kneading Mozzarella curd and stretching it 2 to 80 times. Rheological properties were examined by compression, stress relaxation , and flow property tests. Stringiness was tested by a device specifically designed for this purpose: a standardized string was pulled from the sample at a direction perpendicular to the orientation of the curd and the amount of fibres resulting from this process was quantitated using digital image analysis.

Force-deformation curves implied that the curd prepared by kneading may be considered to be an incompressible viscoelastic body similar to …

Microstructure And Texture Of Khoa, G. R. Patil, A. A. Patel, Paula Allan-Wojtas, Miloslav Kalab

Food Structure

Khoa, a partially dehydrated milk product indigenous to India, was prepared from buffalo milk by boiling it vigorously in an open pan and reducing its volume to approximately 25% within 30 min. The hot semi-solid product (Khoa pat) was held at 20•c for 3 h (fresh, cooled Khoa) or 48 h (swred Khoa); the products were either worked with a pestle in a mortar for 5 min or were left without working.

Structural features of Khoa were studied by light microscopy and electron microscopy. Freshly prepared coo led Khoa had a granular structure consisting of protein granules several hundred micrometers …

Light Microscopy Measurements Of Ice Recrystallization In Frozen Corn Starch Pastes Using Isothermal Freeze Fixation, C. Ferrero, M. N. Martino, N. E. Zaritzky

Food Structure

Isothermal freeze fixation was used to analyze ice recrystallization by light microscopy in a 10 % (W/W) frozen corn starch paste during storage at temperatures in the range of -5 to -20 °C.

Different formulations were tested in order to obtain a suitable fixative for this method of indirect observation of the ice crystals. A solution of formaldehyde, ethanol and water (10:45:45 V:V) was selected because it minimized substitution-induced distortion and contraction of the matrix. The diffusion coefficients of the selected fixative in the frozen system were measured at different temperatures in conditions of unidirectional mass transfer in a semi …

Heat-Induced Structural Changes In Acid-Modified Barley Starch Dispersions, K. Autio, K. Poutanen, T. Suortti, E. Pessa

Food Structure

The effect of heat treatment on the gel formation and microstructure of barley starch dispersions , hydrolyzed with I M HCI for 0.5, 1.0 and 4.0 hours , was studied by dynamic viscoelastic methods and by light microscopy . The effects of acid hydrolysis on the molecular weight of amylopectin and amylose were studied by high -performance liquid chromatography (HPLC) with post-column iodine staining.

Microstructural studies of 8% hydrolyzed barley starch dispersions heated to 90 0 C showed that even a short acid treatment induced considerable changes in the granule structure. The molecular weight of amylopectin decreased substantially. As the …