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Full-Text Articles in Life Sciences

Functional And Morphological Studies Of Mitochondria Exposed To Undecagold Clusters: Biologic Surfaces Labeling With Gold Clusters, E. Valdivia, C. Gabel, J. E. Reardon, C. A. Cajacob, H. Yang, R. S. Wehbi, G. L. Scott, P. A. Frey, L. A. Fahien Jul 1992

Functional And Morphological Studies Of Mitochondria Exposed To Undecagold Clusters: Biologic Surfaces Labeling With Gold Clusters, E. Valdivia, C. Gabel, J. E. Reardon, C. A. Cajacob, H. Yang, R. S. Wehbi, G. L. Scott, P. A. Frey, L. A. Fahien

Scanning Microscopy

This study reports morphological and functional alterations observed in respiring isolated mitochondria when they are exposed to nonpenetrating, positive electrostatically charged synthetic undecagold clusters. Modification of the undecagold clusters positive charges change or prevent the functional effects and the binding to the outside surface of the mitochondria. The mitochondrial functional alterations are dependent on the oxidative phosphorylation capacity of the isolated organelles. The results of these experiments indicate that artificial undecagold may be useful to explore the molecular mechanisms of biological energy transducers which require electric charges separation, ionic fluxes, and electric surface properties.


Scanning Electron Microscopy Structure And Firmness Of Papain Treated Apple Slices, Yaguang Luo, Max E. Patterson, Barry G. Swanson Jan 1992

Scanning Electron Microscopy Structure And Firmness Of Papain Treated Apple Slices, Yaguang Luo, Max E. Patterson, Barry G. Swanson

Food Structure

'Mcintosh' apple (Malus domesrica Borkh.) slices were treated with papain. Textural changes were recorded with an Instron Universal Testing Machine. Structural changes and distribution of microorganisms in apple tissues after treatment were observed with a scanning electron microscope (SEM). Apple slices submerg ed in a 1% papain solution were significantly firmer than apple slices submerged in the distilled water control for a 24 hour period (P < 0.05). Three and four days after slicing , a significantly smaller decay index was observed on the apple slices submerged in papain solution than on the control slices. Under SEM, less severe cell wall breakdown was observed on the apple tissues treated with papain than on apple tissues without treatment. Less spores were also observed on the papain treated apple slices than apple slices without treatment. Apple tissues treated with papain solution and distilled water also demonstrated noticeable st ru ctural differences. The apple tissues treated with papain solution for 18 hours retained the original cell structure while the cells in the apple tissues treated with distilled water collapsed.