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Full-Text Articles in Life Sciences
Simian Virus 40 Host Range/Helper Function Mutations Cause Multiple Defects In Viral Late Gene Expression., Terryl Stacy, Michele Chamberlain, Charles N. Cole
Simian Virus 40 Host Range/Helper Function Mutations Cause Multiple Defects In Viral Late Gene Expression., Terryl Stacy, Michele Chamberlain, Charles N. Cole
Dartmouth Scholarship
Simian virus 40 (SV40) deletion mutants dlA2459 and dlA2475 express T antigens that lack the normal carboxy terminus. These mutants are called host range/helper function (hr/hf) mutants because they form plaques at 37 degrees C on BSC-1 and Vero monkey kidney cell lines but not on CV-1p monkey kidney cells. Wild-type SV40 can provide a helper function to permit growth of human adenoviruses in monkey kidney cells; the hr/hf mutants cannot. Progeny yields of hr/hf mutants are also cold sensitive in all cell lines tested. Patterns of viral macromolecular synthesis in three cell lines (Vero, BSC-1, and CV-1) at three …
Linker Insertion Mutants Of Simian Virus 40 Large T Antigen That Show Trans-Dominant Interference With Wild-Type Large T Antigen Map To Multiple Sites Within The T-Antigen Gene., Jiyue Y. Zhu, Charles N. Cole
Linker Insertion Mutants Of Simian Virus 40 Large T Antigen That Show Trans-Dominant Interference With Wild-Type Large T Antigen Map To Multiple Sites Within The T-Antigen Gene., Jiyue Y. Zhu, Charles N. Cole
Dartmouth Scholarship
Linker insertion mutants affecting the simian virus 40 (SV40) large tumor (T) antigen were constructed by inserting a 12-base-pair oligonucleotide linker into restriction endonuclease cleavage sites located within the early region of SV40. One mutant, with the insertion at amino acid 5, was viable in CV-1p and BSC-1 cells, indicating that sequences very close to the amino terminus of large T could be altered without affecting the lytic infection cycle of SV40. All other mutants affecting large T were not viable. In complementation assays between the linker insertion mutants and either a late-gene mutant, dlBC865, or a host range/helper function …
Immunological Characterization Of An Sds/Kcl Isolated Total Protein Antigen Of Bluetongue Virus Serotype 13, Vicki L. Shore
Immunological Characterization Of An Sds/Kcl Isolated Total Protein Antigen Of Bluetongue Virus Serotype 13, Vicki L. Shore
Undergraduate Honors Capstone Projects
Because of the damaging effects of bluetongue disease on the livestock industry, interest exists in developing a means of inducing passive immunity in infected animals. Antibodies against VP2, the polypeptide responsible for immunoantigenicity in bluetongue virus (BTV), have been found to elicit neutralizing antibodies; however, production of this purified antigen is very costly and thus impractical for commercial purposes. Total viral protein, prepared by a simple, rapid, highly reproducible SDS/KCl method, was injected into rabbits and found to produce antibodies which are useful in immunoblots. However, plaque neutralization assays failed to detect any neutralization activity by these antibodies. Denaturation of …
Microbiological Immunocytochemistry: A Review Of Current Trends And Applications, Julian E. Beesley
Microbiological Immunocytochemistry: A Review Of Current Trends And Applications, Julian E. Beesley
Scanning Microscopy
It has been considered worthwhile to update previous reviews of microbiological immunocytochemistry in order to identify areas of current importance in this continually expanding area of research. Publications in virology, bacteriology and protozoology indicate a continued interest in immunocytochemistry. Deployment of colloidal gold techniques is almost universal in these applications. The post-embedding technique was the most widely applied technique although a few studies employed the immunonegative stain, pre-embedding and immunoreplica techniques, thereby reflecting the use of colloidal gold in all other areas of the biological sciences.
G89-905 Weed Control On Crp Acres (Revised July 1997), Robert N. Klein, Gail A. Wicks, John E. Watkins, Jerry D. Volesky
G89-905 Weed Control On Crp Acres (Revised July 1997), Robert N. Klein, Gail A. Wicks, John E. Watkins, Jerry D. Volesky
University of Nebraska-Lincoln Extension: Historical Materials
Establishing perennial grasses on former cropland presents a challenge. Weed control can be accomplished with herbicides, tillage, burning, mowing, and crop competition. The key to weed control is timeliness. Weeds should be controlled on CRP acres to reduce the risk of seeding failure and eliminate possible reseeding costs. Soil moisture must be available for seed germination, seedling emergence, and establishment to be successful in getting stands of grasses and/or legumes. Weeds can quickly and thoroughly consume soil water throughout the upper soil profile and must be controlled to allow grass and legume seedlings to germinate and emerge. In addition, rapid-growing, …