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Full-Text Articles in Life Sciences

Role Of Glycine And Glyoxylate Decarboxylation In Photorespiratory Co2 Release, David J. Oliver Nov 1981

Role Of Glycine And Glyoxylate Decarboxylation In Photorespiratory Co2 Release, David J. Oliver

David J. Oliver

Mechanically isolated soybean leaf cells metabolized added glycolate by two mechanisms, the direct oxidation of glyoxylate and the decarboxylation of glycine. The rate of glyoxylate oxidation was dependent on the cellular glyoxylate concentration and was linear between 0.58 and 2.66 micromoles glyoxylate per milligram chlorophyll. The rate extrapolated to zero at a concentration of zero. The concentration and, therefore, the rate of oxidation of glyoxylate could be decreased by adding glutamate or serine to the cells. These substrates were amino donors for the transamination of glyoxylate to glycine. In the presence of these amino acids more CO2 was released from …


Formate Oxidation And Oxygen Reduction By Leaf Mitochondria, David J. Oliver Sep 1981

Formate Oxidation And Oxygen Reduction By Leaf Mitochondria, David J. Oliver

David J. Oliver

Mitochondria isolated from the leaves of several plant species were investigated for the presence of NAD-linked formate dehydrogenase. The NADH produced was oxidized by the electron transport sequence and was coupled to ATP synthesis. The amounts of formate dehydrogenase, and, thereby, the capacity for formate-dependent O2 uptake, varied greatly among species. While no activity was detectable in mitochondria from soybean leaves, the rate of formate oxidation by spinach mitochondria was about one-half the rate of malate oxidation. In spinach, only mitochondria from green tissues oxidized formate. These last two observations raise questions as to the role of this reaction and …


Effects Of Three Vesicular-Arbuscular Mycorrhizal Fungi On Sweetgum Seedlings From Nine Mother Trees, Richard C. Schultz, Paul P. Kormanik, William C. Bryan Jun 1981

Effects Of Three Vesicular-Arbuscular Mycorrhizal Fungi On Sweetgum Seedlings From Nine Mother Trees, Richard C. Schultz, Paul P. Kormanik, William C. Bryan

Richard C. Schultz

Soil in microplots was infested with three vesicular-arbuscular (VA) symbionts then sown with seed of half-sib progeny from nine sweetgum mother trees. The VA treatments were Glomus fasciculatus, Glomus spp. (a mixture containing both Glomus mosseae and Glomus etunicatus), or a VA mixture of several fungi from the genera Glomus and Gigaspora. Before the seed was sown, all plots had calcium standardized and received an application of commercial fertilizer. During the growing season, NH4NO3 was applied to all plots in equal portions. Mycorrhizal seedlings with G. fasciculatus were slightly but not significantly larger in both height and root-collar diameter than …