Life Sciences Commons^™

Magnetosome Gene Duplication As An Important Driver In The Evolution Of Magnetotaxis In The Alphaproteobacteria, Haijian Du, Wenyan Zhang, Wensi Zhang, Weijia Zhang, Hongmiao Pan, Yongxin Pan, Dennis A. Bazylinski, Long-Fei Wu, Tian Xiao, Wei Lin

Life Sciences Faculty Research

The evolution of microbial magnetoreception (or magnetotaxis) is of great interest in the fields of microbiology, evolutionary biology, biophysics, geomicrobiology, and geochemistry. Current genomic data from magnetotactic bacteria (MTB), the only prokaryotes known to be capable of sensing the Earth’s geomagnetic field, suggests an ancient origin of magnetotaxis in the domain Bacteria. Vertical inheritance, followed by multiple independent magnetosome gene cluster loss, is considered to be one of the major forces that drove the evolution of magnetotaxis at or above the class or phylum level, although the evolutionary trajectories at lower taxonomic ranks (e.g., within the class level) remain largely …

Spatial And Temporal Variation Of Offshore Wind Power And Its Values Along The Central California Coast, Yi-Hui Wang, Ryan K. Walter, Crow White, Matthew D. Kehrli, Stephen F. Hamilton, Patrick H. Soper, Benjamin I. Ruttenberg

Physics

The analysis of the spatiotemporal variability of wind power remains limited during the planning stage of an offshore wind farm. This study provides a framework to investigate how offshore wind power varies along the Central California Coast over diurnal and seasonal time scales, which is critical for reliability and functionality of the grid system. We find that offshore wind power in this region peaks during evening hours across all seasons and maximizes in spring and summer. The timing of peak offshore wind power production better aligns with that of peak demand across California than solar and land-based wind power production, …

Data Of Soil, Vegetation And Bird Species Found On Double-Crested Cormorant Colonies In The Southeastern United States, Leah Moran Veum, Brian S. Dorr, Katie Hanson-Dorr, R. J. Moore, Scott A. Rush

United States Department of Agriculture Wildlife Services: Staff Publications

This data article provides the methods and procedures followed to collect and analyse soil, vegetation and bird data on three different treatment islands in Guntersville Reservoir, Alabama. Samples were collected from randomly selected plot points from islands that were placed into three different treatment types: Colony (currently occupied by Double-crested Cormorants) (Phalacrocorax auritus; n 1⁄4 5), Historic (historically occupied by cormorants and currently abandoned; n 1⁄4 3) and Reference (never occupied by cormorants; n 1⁄4 4). We compared vegetation and tree metrics such as structure and diversity, as well as soil chemistry and bird diversity and communities among islands within …

4d Electron Microscopy Of T Cell Activation., Yue Lu, Byung-Kuk Yoo, Alphonsus H C Ng, Jungwoo Kim, Sinchul Yeom, Jau Tang, Milo M Lin, Ahmed H Zewail, James R Heath

Articles, Abstracts, and Reports

T cells can be controllably stimulated through antigen-specific or nonspecific protocols. Accompanying functional hallmarks of T cell activation can include cytoskeletal reorganization, cell size increase, and cytokine secretion. Photon-induced near-field electron microscopy (PINEM) is used to image and quantify evanescent electric fields at the surface of T cells as a function of various stimulation conditions. While PINEM signal strength scales with multiple of the biophysical changes associated with T cell functional activation, it mostly strongly correlates with antigen-engagement of the T cell receptors, even under conditions that do not lead to functional T cell activation. PINEM image analysis suggests that …

Running Economy - A Comprehensive Review For Passive Force Generation, Christos Ziliaskoudis, Song-Young Park, Sang-Ho Lee

Health and Kinesiology Faculty Publications

Running economy is considered a major determinant of distance running performance. Enhancing the body’s ability for passive force generation could have a positive effect on running economy by minimizing the energy cost required for the propulsion of the body. Thus, the purpose of this comprehensive review was to provide a list of modifiable factors that promote this ability. The interest was focused on lower-limb stiffness, as it is a factor of great influence and at the same time can be modified with training and specific biomechanical adjustments. Although it appears that no clear instructions can be provided to athletes and …

Repeated Vaginal Exposures To The Common Cosmetic And Household Preservative Methylisothiazolinone Induce Persistent, Mast Cell-Dependent Genital Pain In Nd4 Mice, Erica Arriaga-Gomez, Jacyln Kline, Elizabeth Emanuel, Nefeli Neamonitaki, Tenzin Yangdon, Hayley Zacheis, Dogukan Pasha, Jinyoung Lim, Susan Bush, Beebie Boo, Hanna Mengistu, Ruby Kinnamon, Robin Shields-Cutler, Wattenberg Wattenberg, Devavani Chatterjea

Faculty Publications

A history of allergies doubles the risk of vulvodynia-a chronic pain condition of unknown etiology often accompanied by increases in numbers of vulvar mast cells. We previously established the biological plausibility of this relationship in mouse models where repeated exposures to the allergens oxazolone or dinitrofluorobenzene on the labiar skin or inside the vaginal canal of ND4 Swiss Webster outbred mice led to persistent tactile sensitivity and local increases in mast cells. In these models, depletion of mast cells alleviated pain. While exposure to cleaning chemicals has been connected to elevated vulvodynia risk, no single agent has been linked to …

Global Imprint Of Mycorrhizal Fungi On Whole-Plant Nutrient Economics, Colin Averill, Jennifer M. Bhatnagar, Michael C. Dietze, William D. Pearse, Stephanie N. Kivlin

Biology Faculty Publications

Mycorrhizal fungi are critical members of the plant microbiome, forming a symbiosis with the roots of most plants on Earth. Most plant species partner with either arbuscular or ectomycorrhizal fungi, and these symbioses are thought to represent plant adaptations to fast and slow soil nutrient cycling rates. This generates a second hypothesis, that arbuscular and ectomycorrhizal plant species traits complement and reinforce these fungal strategies, resulting in nutrient acquisitive vs. conservative plant trait profiles. Here we analyzed 17,764 species level trait observations from 2,940 woody plant species to show that mycorrhizal plants differ systematically in nitrogen and phosphorus economic traits. …

Venom Diversity And Evolution In The Most Divergent Cone Snail Genus Profundiconus, Guilia Fassio, Maria Vittoria Modica, Lou Mary, Paul Zaharias, Alexander E. Fedosov, Juliette Gorson, Yuri I. Kantor, Mandë E. Holford, Nicolas Puillandre

Publications and Research

Profundiconus is the most divergent cone snail genus and its unique phylogenetic position, sister to the rest of the family Conidae, makes it a key taxon for examining venom evolution and diversity. Venom gland and foot transcriptomes of Profundiconus cf. vaubani and Profundiconus neocaledonicus were de novo assembled, annotated, and analyzed for differential expression. One hundred and thirty-seven venom components were identified from P. cf. vaubani and 82 from P. neocaledonicus, with only four shared by both species. The majority of the transcript diversity was composed of putative peptides, including conotoxins, profunditoxins, turripeptides, insulin, and prohormone-4. However, there were also …

Fetal Programming Of Intrauterine Growth Restricted Skeletal Muscle Stem Cell Function, Micah Most

Honors Theses

Intrauterine growth restriction (IUGR) reduces skeletal muscle mass in fetuses and offspring. The objective of this study was to determine if myoblasts (skeletal muscle stem cells) from maternal inflammation induced intrauterine growth-restricted (MI-IUGR) fetuses are less responsive to proliferation-stimulating factors in culture. Ovine MI-IUGR fetal myoblasts were isolated at 125 days of gestational age (dGA, term = 150 dGA), grown for 72 hours in complete growth media spiked with insulin, tumor necrosis factor α (TNFα), or unspiked (basal), and analyzed for ex vivo proliferative capacity via a 2 hour EdU pulse. A second set of myoblasts were differentiated in differentiation …

Cercarial Parasitism Of Freshwater Snails In Sandhills Ponds Versus Streams, Caroline Emily Spethman

Honors Theses

Parasite prevalence was calculated for all aquatic snails collected from four freshwater localities in Western Nebraska from Summer 2016 to 2019: Dunwoody Pond, Haythorn Ranch, Neven’s Restoration, and Breen’s Flyaway. With data of increasing trematode cercarial infection specifically at Dunwoody Pond, this community was further studied. In Summer 2019, a total of 217 freshwater snails representing species of Physa sp., Stagnicola elodes (Say,1821), and Helisoma anceps (Menke,1830) were collected from Dunwoody Pond and Dunwoody stream, near Ogallala, Nebraska. Eighty-one snails were collected from Dunwoody Pond, 76 snails were collected 31 meters upstream, and 60 snails were collected 61 meters upstream …

Antibiotics Overuse And Bacterial Resistance, Mir Saleem, Brett Deters, Adam De La Bastide, Martha Korzen

Biology Faculty Articles

Antibiotic usage has become very widespread, as they are used to treat so many infectious diseases today. Antimicrobial agents exert their actions via different mechanisms including blockage of cell wall synthesis, interference of protein and/or nucleic acid synthesis, interruption of cell membrane structure, and inhibition of a metabolic pathway. The treatment of bacterial infections with antimicrobial agents has become more difficult due to the capability of bacteria to develop resistance to antibiotics. Erroneous diagnosing, misconceptions, and improper physician-patient dynamics have led to overuse of antibiotics and the emergence of drug-resistant bacteria. Bacterial colonies have been shown to confer advantageous genetic …

Oyster Reefs In Northern Gulf Of Mexico Estuaries Harbor Diverse Fish And Decapod Crustacean Assemblages: A Meta-Synthesis, Megan K. La Peyre, Danielle Aguilar Marshall, Lindsay S. Miller

Faculty Publications

Oyster reefs provide habitat for numerous fish and decapod crustacean species that mediate ecosystem functioning and support vibrant fisheries. Recent focus on the restoration of eastern oyster (Crassostrea virginica) reefs stems from this role as a critical ecosystem engineer. Within the shallow estuaries of the northern Gulf of Mexico (nGoM), the eastern oyster is the dominant reef building organism. This study synthesizes data on fish and decapod crustacean occupancy of oyster reefs across nGoM with the goal of providing management and restoration benchmarks, something that is currently lacking for the region. Relevant data from 23 studies were identified, representing data …

Perceptions Of Health Professionals Regarding Minimally Invasive Tissue Sampling (Mits) To Identify The Cause Of Death In Stillbirths And Neonates: Results From A Qualitative Study, Anam Shahil Feroz, Anum Shiraz Ali, Mohsina Noor Ibrahim, Elizabeth M. Mcclure, Shiyam Sunder Tikmani, Sayyeda Reza, Zahid Abbasi, Afia Zafar, Sameen Siddiqi, Sarah Saleem

Community Health Sciences

Background: Pakistan is considered to be one of the riskiest places in the world for childbirth as measured by its high stillbirth and neonatal mortality rates. Complete diagnostic autopsy remains the gold standard to determine the cause of death (CoD); however, it is not routinely implemented due to religious objections, sociocultural beliefs, limited resources and low demand from physicians and families. Recently, minimally invasive tissue sampling (MITS) using needle biopsies of multiple tissues to obtain tissue for histological examination and organism identification with PCR has been developed and promoted to determine CoD in low-resource areas. To ensure successful implementation of …

Comparisons Of Mean Length-Based Mortality Estimators And Age-Structured Models For Six Southeastern Us Stocks, Quang C. Huynh, Nancie J. Cummings, John M. Hoenig

VIMS Articles

Length-based mortality estimators have been developed as alternative assessment methods for data-limited stocks. We compared mortality estimates from three methodologically related mean length-based methods to those from an age-structured model (ASM). We estimated fishing mortality and determined overfishing status, i.e. if F/F_MSY > 1, for six stocks which support important recreational and commercial fisheries in the southeastern United States. The similarities in historical fishing mortality between the length-based methods and the most recent assessments varied among the case studies, but the classification of overfishing status in the terminal year did not differ based on the choice of model …

An Annotated Checklist Of The Scarabaeoidea (Insecta: Coleoptera) Of The Guianas, Auke J. Hielkema, Meindert A. Hielkema

Insecta Mundi

A checklist of the Scarabaeoidea (except Passalidae) of the Guianas (Guyana, Suriname and French Guiana) is presented for the first time. Both recorded and potential species are included, the latter defined as being recorded from Brazil north of the Amazon/Rio Negro main channel or from Venezuela east of the Orinoco main channel. The checklist contains a total of 930 species and subspecies, with 265 validly recorded from Guyana (eight new country records), 388 from Suriname (146 new country records), 531 from French Guiana (four new country records) and 177 additional species recorded from contiguous areas of neighboring Brazil and Venezuela. …

Genetic Structure Of Mycoplasma Ovipneumoniae Informs Pathogen Spillover Dynamics Between Domestic And Wild Caprinae In The Western United States, Pauline L. Kamath, Kezia Manlove, E. Frances Cassirer, Paul C. Cross, Thomas E. Besser

Ecology Center Publications

Spillover diseases have significant consequences for human and animal health, as well as wildlife conservation. We examined spillover and transmission of the pneumonia-associated bacterium Mycoplasma ovipneumoniae in domestic sheep, domestic goats, bighorn sheep, and mountain goats across the western United States using 594 isolates, collected from 1984 to 2017. Our results indicate high genetic diversity of M. ovipneumoniae strains within domestic sheep, whereas only one or a few strains tend to circulate in most populations of bighorn sheep or mountain goats. These data suggest domestic sheep are a reservoir, while the few spillovers to bighorn sheep and mountain goats can …

S1e1: What Is Edna And How Will It Change Maine’S Coastal Communities?, Ron Lisnet, Michael Kinnison

The Maine Question

From streams and ponds to the Gulf of Maine, water defines the state of Maine in many ways. For thousands of years, counting or harvesting the plants and animals in those bodies of water has been pretty low-tech, usually involving nets of some sort. Now a new technology can do this counting using DNA and this tool will revolutionize and expand how this work is done. Beyond that it will allow the public, school groups, coastal residents and others to contribute as citizen scientists and it will enhance Maine’s workforce and promote high-tech jobs as well. Michael Kinnison, a professor …

Assessing Undergraduate Student And Faculty Views On Animal Research: What Do They Know, Whom Do They Trust, And How Much Do They Care?, Eric P. Sandgren, Robert Streiffer, Jennifer Dykema, Nadia Assad, Jackson Moberg

Attitudes Toward Animal Research Collection

Research using animals is controversial. To develop sound public outreach and policy about this issue, we need information about both the underlying science and people’s attitudes and knowledge. To identify attitudes toward this subject at the University of Wisconsin-Madison, we developed and administered a survey to undergraduate students and faculty. The survey asked respondents about the importance of, their confidence in their knowledge about, and who they trusted to provide information on animal research. Findings indicated attitudes varied by academic discipline, especially among faculty. Faculty in the biological sciences, particularly those who had participated in an animal research project, reported …

Geographical Information Systems In Python Nrs 528, Michael Cerbo

Library Impact Statements

No abstract provided.

Diversification Through ‘Fun In The Farm’: Analyzing Structural Factors Affecting Agritourism In Tennessee, Aditya R. Khanal, Ummey Honey, Omobolaji Omobitan

Agricultural and Environmental Sciences Faculty Research

With recent development, agritourism – visiting a working agricultural setting such as farm and ranch for leisure, recreation, or educational purposes – is gaining popularity in the US. Agritourism can be considered as a viable alternative agricultural enterprise to enhance income and sustainability of farms. This study maps agritourism locations and analyzes the structural factors affecting agritourism location and establishments. Using zip-code level location information, county-level data from Tennessee and spatial regression models, we found significant effects of rural factors, demographics, and educational-, economic-, and cultural- capital factors and their interactions on the location and establishment of agritourism farms. The …

Linalool Acts As A Fast And Reversible Anesthetic In Hydra, T. Goel, R. Wang, S Martin, Elizabeth Y. Lanphear , '19, Eva-Maria S. Collins

Biology Faculty Works

The ability to make transgenic Hydra lines has allowed for quantitative in vivo studies of Hydra regeneration and physiology. These studies commonly include excision, grafting and transplantation experiments along with high-resolution imaging of live animals, which can be challenging due to the animal’s response to touch and light stimuli. While various anesthetics have been used in Hydra studies, they tend to be toxic over the course of a few hours or their long-term effects on animal health are unknown. Here, we show that the monoterpenoid alcohol linalool is a useful anesthetic for Hydra. Linalool is easy to use, non-toxic, fast …

Identifiying High Risk Areas Of Zika Virus Infection By Meteorological Factors In Columbia, Lung-Chang Chien, Francisco Sy, Adriana Perez

Environmental & Occupational Health Faculty Publications

Background Several Zika virus (ZIKV) outbreaks have occurred since October 2015. Because there is no effective treatment for ZIKV infection, developing an effective surveillance and warning system is currently a high priority to prevent ZIKV infection. Despite Aedes mosquitos having been known to spread ZIKV, the calculation approach is diverse, and only applied to local areas. This study used meteorological measurements to monitor ZIKV infection due to the high correlation between climate change and Aedes mosquitos and the convenience to obtain meteorological data from weather monitoring stations. Methods This study applied the Bayesian structured additive regression modeling approach to include …

Patient And Provider Perspectives On How Trust Influences Maternal Vaccine Acceptance Among Pregnant Women In Kenya, Stacy W. Nganga, Nancy A. Otieno, Maxwell Adero, Dominic Ouma, Sandra S. Chaves, Jennifer R. Verani, Marc-Alain Widdowson, Andrew Wilson, Irina Bergenfeld, Courtni Andrews, Vincent L. Fenimore, Ines Gonzalez-Casanova, Paula M. Frew, Saad B. Omer, Fauzia A. Malik

Environmental & Occupational Health Faculty Publications

Background Pregnant women and newborns are at high risk for infectious diseases. Altered immunity status during pregnancy and challenges fully vaccinating newborns contribute to this medical reality. Maternal immunization is a strategy to protect pregnant women and their newborns. This study aimed to find out how patient-provider relationships affect maternal vaccine uptake, particularly in the context of a lower middle- income country where limited research in this area exists. Methods We conducted semi-structured, in-depth narrative interviews of both providers and pregnant women from four sites in Kenya: Siaya, Nairobi, Mombasa, and Marsabit. Interviews were conducted in either English or one …

Fate Of The Organophosphate Insecticide, Chlorpyrifos, In Leaves, Soil, And Air Following Application, Supta Das, Kimberly J. Hageman, Madeleine Taylor, Sue Michelsen-Heath, Ian Stewart

Chemistry and Biochemistry Faculty Publications

A field study was conducted to further our understanding about the fate and transport of the organophosphate insecticide, chlorpyrifos, and its degradation product, chlorpyrifos oxon. Leaf, soil and air sampling was conducted for 21 days after chlorpyrifos application to a field of purple tansy (Phacelia tanacetifolia). Air samples were collected using a high-volume air sampler (HVAS) and seven battery-operated medium-volume active air samplers placed around the field and on a 500-m transect extending away from the field. Chlorpyrifos was detected every day of the sampling period in all matrices, with concentrations decreasing rapidly after application. Chlorpyrifos oxon was …

Biochar Enhances Nitrous Oxide Reduction In Acidic But Not In Near-Neutral Ph Soil, Engil Isadora Pujol Pereira, Jerome Lechot, Rafaela Feola Conz, Abmael Da Silva Cardoso, Johan Six

School of Earth, Environmental, and Marine Sciences Faculty Publications and Presentations

We quantified nitrous oxide (N2O) fluxes and total denitrification (N2O + N2) in an acidic (Ferralsol) and a near-neutral pH soil (Cambisol) to determine whether biochar’s alkalinization effect could be the mechanism inducing potential reductions in N2O fluxes. In Ferralsol, decreases in N2O emissions and in the N2O to N2O + N2 ratio were observed in both biochar and lime treatments. In Cambisol, neither biochar nor lime decreased N2O emissions, despite significantly increasing soil pH. The abundance and community structure of nosZ gene-bearing microorganisms indicated that gene abundances did not explain biochar effects, but a higher diversity of nosZ gene-bearing …

From The Human To The Planetary: Speculative Futures Of Care, Miriam Ticktin

Publications and Research

This is largely a theoretical, speculative essay that takes on the question of what ‘care’ looks like at a moment when climate change is increasingly taking center stage in public and political discussions. Starting with two new practices, namely, humanitarian care for nonhumans and One Health collaborations, I seek to determine what forms of political care can incorporate the well-being of future generations and future iterations of the earth. After an exploration of One Health as an approach to planetary care, I ask what its parts enable us to think, despite its limitations; I focus on the new human-nonhuman assemblages …

Corridors Or Risk? Movement Along, And Use Of, Linear Features Varies Predictably Among Large Mammal Predator And Prey Species, Melanie Dickie, Scott R. Mcnay, Glenn D. Sutherland, Michael Cody, Tal Avgar

Wildland Resources Faculty Publications

1. Space‐use behaviour reflects trade‐offs in meeting ecological needs and can have consequences for individual survival and population demographics. The mechanisms underlying space use can be understood by simultaneously evaluating habitat selection and movement patterns, and fine‐resolution locational data are increasing our ability to do so.
2. We use high‐resolution location data and an integrated step‐selection analysis to evaluate caribou, moose, bear, and wolf habitat selection and movement behaviour in response to anthropogenic habitat modification, though caribou data were limited. Space‐use response to anthropogenic linear features (LFs) by predators and prey is hypothesized to increase predator hunting efficiency and is thus believed …

Core–Shell Nanospheres Behind The Blue Eyes Of The Bay Scallop Argopecten Irradians, Olivia K. Harris, Alexandra C.N. Kingston, Caitlin S. Wolfe, Soumitra Ghoshroy, Sönke Johnsen, Daniel Isaac Speiser

Faculty Publications

The bay scallop Argopecten irradians (Mollusca: Bivalvia) has dozens of iridescent blue eyes that focus light using mirror-based optics. Here, we test the hypothesis that these eyes appear blue because of photonic nanostructures that preferentially scatter short-wavelength light. Using transmission electron microscopy, we found that the epithelial cells covering the eyes of A. irradians have three distinct layers: an outer layer of microvilli, a middle layer of random close-packed nanospheres and an inner layer of pigment granules. The nanospheres are approximately 180 nm in diameter and consist of electron-dense cores approximately 140 nm in diameter surrounded by less electron-dense shells …

An Illustration Of Farm Program Decisions And Impacts, Bradley D. Lubben

Cornhusker Economics

When the 2018 Farm Bill was signed last December, producers could look ahead to implementation and the coming decision between enrollment under the Agricultural Risk Coverage (ARC) program or the Price Loss Coverage (PLC) program. While the ARC and PLC programs carried over from the 2014 Farm Bill with relatively modest changes, the substantial drop in market prices and outlook since 2014 pointed toward a widespread shift in enrollment away from ARC and toward PLC due to the increased relevance of the price safety net.

However, with this year’s extreme weather events, concerns over crop production, and hopes for improved …

Testing Protocols For Surveying Captive Limulus Polyphemus Microbiomes, Fabian Leija, Sean A. Neiswenter Dr.

LSAMP Poster Presentations

Limulus polyphemus, the Atlantic horseshoe crab, is a marine arthropod that primarily resides in the shallow intertidal zone of brackish water, water where high salinity seawater meets fresh water. The ecological niche that horseshoe crabs reside in makes them an ideal animal to be placed in an aquarium touch-tank, only needing a shallow pool of water to live comfortably as well as having a tough chitin carapace that won’t be easily damaged by visitors touching them. However, the implications of these human-animal interactions are severely unexplored with limited research being conducted regarding touch-tanks. In contrast, the consequences and hygienic practices …