Life Sciences Commons^™

The Antimicrobial Activity And Cellular Pathways Targeted By P-Anisaldehyde And Epigallocatechin Gallate In The Opportunistic Human Pathogen Pseudomonas Aeruginosa, Yetunde Adewumni, Sanchirmaa Namjilsuren, William D. Walker, Dahlia N. Amato, Douglas V. Amato, Olga V. Mavrodi, Derek L. Patton, Dmitri V. Mavrodi

Faculty Publications

Plant-derived aldehydes are constituents of essential oils that possess broad-spectrum antimicrobial activity and kill microorganisms without promoting resistance. In our previous study, we incorporated p-anisaldehyde from star anise into a polymer network called PANDAs (Pro-Antimicrobial Networks via Degradable Acetals) and used it as a novel drug delivery platform. PANDAs released p-anisaldehyde upon a change in pH and humidity, and controlled growth of the multi-drug resistant pathogen Pseudomonas aeruginosa PAO1. In this study, we identified cellular pathways targeted by p-anisaldehyde, by generating 10,000 transposon mutants of PAO1 and screened them for hypersensitivity to p-anisaldehyde. To improve the …

Argulus From The Pascagoula River, Ms, Usa, With An Emphasis On Those Of The Threatened Gulf Sturgeon, Acipenser Oxyinchus Desotoi, Michael J. Andres, Jeremy M. Higgs, Paul O. Grammer, Mark S. Peterson

Faculty Publications

Species of Argulus (Branchiura Thorell, 1864) are common ectoparasites of freshwater, estuarine, and marine fishes. Argulid identification and taxonomy is often confusing because many species are reported to parasitize multiple host species, have similar morphological characters, and come from various salinity regimes. Gulf sturgeon is an anadromous fish natal to drainages in the north-central Gulf of Mexico, and as with many endangered species, has a poorly documented parasite community. During Gulf sturgeon tagging and monitoring studies (2016–2019) in the Pascagoula River, MS, USA, species of Argulus were collected from Gulf sturgeon as well as other incidentally captured fishes. Argulus flavescens …

Importance Of Refractory Ligands And Their Photodegradation For Iron Oceanic Inventories And Cycling, Christel Hassler, Damien Cabanes, Sonia Blanco-Ameijeiras, Sylvia G. Sander, Ronald Benner

Faculty Publications

Iron is an essential micronutrient that limits primary production in up to 40% of the surface ocean and influences carbon dioxide uptake and climate change. Dissolved iron is mostly associated with loosely characterised organic molecules, called ligands, which define key aspects of the iron cycle such as its residence time, distribution and bioavailability to plankton. Models based on in situ ligand distributions and the behaviour of purified compounds include long-lived ligands in the deep ocean, bioreactive ligands in the surface ocean and photochemical processes as important components of the iron cycle. Herein, we further characterise biologically refractory ligands in dissolved …

Evaluation Of Detached Strawberry Leaves For Anthracnose Disease Severity Using Image Analysis And Visual Ratings, Melinda A. Miller-Butler, Barbara J. Smith, Kenneth J. Curry, Eugene K. Blythe

Faculty Publications

Inoculation of detached strawberry leaves with Colletotrichum species may provide an accurate, rapid, nondestructive method of identifying anthracnose-resistant germplasm. The purpose of this study was to statistically compare two methods (visual and image analysis) of evaluating disease severity of strawberry germplasm screened for anthracnose resistance. Detached leaves of 77 susceptible and resistant strawberry clones were inoculated with one Colletotrichum gloeosporioides (Penz.) Penz. & Sacc. and two C. fragariae A. N. Brooks isolates. Anthracnose disease symptoms on each leaf were assessed quantitatively via computer-based image analysis to determine percentage lesion area and qualitatively by two independent raters using a visual disease …

Distinguishing Anuran Species By High-Resolution Melting Analysis Fo The Coi Barcode (Coi-Hrm), Steven Everman, Shiao Y. Wang

Faculty Publications

Taxonomic identification can be difficult when two or more species appear morphologically similar. DNA barcoding based on the sequence of the mitochondrial cytochrome c oxidase 1 gene (COI) is now widely used in identifying animal species. High‐resolution melting analysis (HRM) provides an alternative method for detecting sequence variations among amplicons without having to perform DNA sequencing. The purpose of this study was to determine whether HRM of the COI barcode can be used to distinguish animal species. Using anurans as a model, we found distinct COI melting profiles among three congeners of both Lithobates spp. and Hyla spp. Sequence variations …

Cgste11 Mediates Cross Tolerance To Multiple Environmental Stressors In Candida Glabrata, Mian Huang, Jibran Khan, Manpreet Kaur, Julian Daniel Torres Vanega, Orlando Andres Aguilar Patiño, Anand Ramasubramanian, Katy Kao

Faculty Publications

Candida glabrata is a human commensal and an opportunistic human fungal pathogen. It is more closely related to the model yeast Saccharomyces cerevisiae than other Candida spp. Compared with S. cerevisiae, C. glabrata exhibits higher innate tolerance to various environmental stressors, including hyperthermal stress. Here we investigate the molecular mechanisms of C. glabrata adaptation to heat stress via adaptive laboratory evolution. We show that all parallel evolved populations readily adapt to hyperthermal challenge (from 47 °C to 50 °C) and exhibit convergence in evolved phenotypes with extensive cross-tolerance to various other environmental stressors such as oxidants, acids, and alcohols. Genome …

The Impact Of Msaabcr On Sara-Associated Phenotypes Is Different In Divergent Clinical Isolates Of Staphylococcus Aureus, Joseph S. Rom, Aura M. Ramirez, Karen E. Beenken, Gyan S. Sahukhal, Mohamed O. Elasri, Mark S. Smeltzer

Faculty Publications

The staphylococcal accessory regulator (sarA) plays an important role in Staphylococcus aureus infections including osteomyelitis, and the msaABCR operon has been implicated as an important factor in modulating expression of sarA. Thus, we investigated the contribution of msaABCR to sarA-associated phenotypes in the S. aureus clinical isolates LAC and UAMS-1. Mutation of msaABCR resulted in reduced production of SarA and a reduced capacity to form a biofilm in both strains. Biofilm formation was enhanced in a LAC msa mutant by restoring the production of SarA, but this was not true in a UAMS-1 msa mutant. Similarly, …

Testing Predictability Of Disease Outbreaks With A Simple Model Of Pathogen Biogeography, Tad Dallas, Colin J. Carlson, Timothée Poisot

Faculty Publications

Predicting disease emergence and outbreak events is a critical task for public health professionals and epidemiologists. Advances in global disease surveillance are increasingly generating datasets that are worth more than their component parts for prediction-oriented work. Here, we use a trait-free approach which leverages information on the global community of human infectious diseases to predict the biogeography of pathogens through time. Our approach takes pairwise dissimilarities between countries’ pathogen communities and pathogens’ geographical distributions and uses these to predict country–pathogen associations. We compare the success rates of our model for predicting pathogen outbreak, emergence and re-emergence potential as a function …

Analysis Of The Cody Rnome Reveals Rsad As A Stress-Responsive Riboregulator Of Overflow Metabolism In Staphylococcus Aureus, Yoann Augagneur, Alyssa N. King, Noëlla Germain-Amiot, Mohamed Sassi, John W. Fitzgerald, Gyan S. Sahukhal, Mohamed O. Elasri, Brice Felden, Shaun R. Brinsmade

Faculty Publications

In Staphylococcus aureus, the transcription factor CodY modulates the expression of hundreds of genes, including most virulence factors, in response to the availability of key nutrients like GTP and branched‐chain amino acids. Despite numerous studies examining how CodY controls gene expression directly or indirectly, virtually nothing is known about the extent to which CodY exerts its effect through small regulatory RNAs (sRNAs). Herein, we report the first set of sRNAs under the control of CodY. We reveal that staphylococcal sRNA RsaD is overexpressed >20‐fold in a CodY‐deficient strain in three S. aureus clinical isolates and in S. epidermidis. …

Avian Ecological Succession In The Amazon: A Long-Term Case Study Following Experimental Deforestation, Cameron L. Rutt, Vitek Jirinec

Faculty Publications

Approximately 20% of the Brazilian Amazon has now been deforested, and the Amazon is currently experiencing the highest rates of deforestation in a decade, leading to large-scale land-use changes. Roads have consistently been implicated as drivers of ongoing Amazon deforestation and may act as corridors to facilitate species invasions. Long-term data, however, are necessary to determine how ecological succession alters avian communities following deforestation and whether established roads lead to a constant influx of new species. We used data across nearly 40 years from a large-scale deforestation experiment in the central Amazon to examine the avian colonization process in a …

Incubation Recess Behaviors Influence Nest Survival Of Wild Turkeys, Nicholas W. Bakner, Bret A. Collier, Landon R. Schofield

Faculty Publications

In ground nesting upland birds, reproductive activities contribute to elevated predation risk, so females presumably use multiple strategies to ensure nest success. Identification of drivers reducing predation risk has primarily focused on evaluating vegetative conditions at nest sites, but behavioral decisions manifested through movements during incubation may be additional drivers of nest survival. However, our understanding of how movements during incubation impact nest survival is limited for most ground nesting birds. Using GPS data collected from female Eastern Wild Turkeys (n = 206), we evaluated nest survival as it relates to movement behaviors during incubation, including recess frequency, distance traveled …

Impact Of Hydration Status On Electromyography And Ratings Of Perceived Exertion During The Vertical Jump, Paul T. Donahue, Samuel J. Wilson, Charles C. Williams, Melinda Valliant, John C. Garner

Faculty Publications

Background: The vertical jumping task is commonly used to assess lower-body power output in athletic populations, in addition to being commonly used to during investigations of hydration and anaerobic performance. Changes in neuromuscular function during a hypohydrated state have been proposed as a potential mechanism to decreases in anaerobic performance.

Objectives: The primary purpose of this investigation was to examine the impact of hydration state on electromyography during the vertical jumping task.

Methods: Twenty recreationally trained males were tested in three hydration conditions (hypohydrated, euhydrated, and control). Testing included maximal voluntary contractions of the vastus lateralis, vastus …

Repeated Vaginal Exposures To The Common Cosmetic And Household Preservative Methylisothiazolinone Induce Persistent, Mast Cell-Dependent Genital Pain In Nd4 Mice, Erica Arriaga-Gomez, Jacyln Kline, Elizabeth Emanuel, Nefeli Neamonitaki, Tenzin Yangdon, Hayley Zacheis, Dogukan Pasha, Jinyoung Lim, Susan Bush, Beebie Boo, Hanna Mengistu, Ruby Kinnamon, Robin Shields-Cutler, Wattenberg Wattenberg, Devavani Chatterjea

Faculty Publications

A history of allergies doubles the risk of vulvodynia-a chronic pain condition of unknown etiology often accompanied by increases in numbers of vulvar mast cells. We previously established the biological plausibility of this relationship in mouse models where repeated exposures to the allergens oxazolone or dinitrofluorobenzene on the labiar skin or inside the vaginal canal of ND4 Swiss Webster outbred mice led to persistent tactile sensitivity and local increases in mast cells. In these models, depletion of mast cells alleviated pain. While exposure to cleaning chemicals has been connected to elevated vulvodynia risk, no single agent has been linked to …

Oyster Reefs In Northern Gulf Of Mexico Estuaries Harbor Diverse Fish And Decapod Crustacean Assemblages: A Meta-Synthesis, Megan K. La Peyre, Danielle Aguilar Marshall, Lindsay S. Miller

Faculty Publications

Oyster reefs provide habitat for numerous fish and decapod crustacean species that mediate ecosystem functioning and support vibrant fisheries. Recent focus on the restoration of eastern oyster (Crassostrea virginica) reefs stems from this role as a critical ecosystem engineer. Within the shallow estuaries of the northern Gulf of Mexico (nGoM), the eastern oyster is the dominant reef building organism. This study synthesizes data on fish and decapod crustacean occupancy of oyster reefs across nGoM with the goal of providing management and restoration benchmarks, something that is currently lacking for the region. Relevant data from 23 studies were identified, representing data …

Core–Shell Nanospheres Behind The Blue Eyes Of The Bay Scallop Argopecten Irradians, Olivia K. Harris, Alexandra C.N. Kingston, Caitlin S. Wolfe, Soumitra Ghoshroy, Sönke Johnsen, Daniel Isaac Speiser

Faculty Publications

The bay scallop Argopecten irradians (Mollusca: Bivalvia) has dozens of iridescent blue eyes that focus light using mirror-based optics. Here, we test the hypothesis that these eyes appear blue because of photonic nanostructures that preferentially scatter short-wavelength light. Using transmission electron microscopy, we found that the epithelial cells covering the eyes of A. irradians have three distinct layers: an outer layer of microvilli, a middle layer of random close-packed nanospheres and an inner layer of pigment granules. The nanospheres are approximately 180 nm in diameter and consist of electron-dense cores approximately 140 nm in diameter surrounded by less electron-dense shells …

Gut Dna Virome Diversity And Its Association With Host Bacteria Regulate Inflammatory Phenotype And Neuronal Immunotoxicity In Experimental Gulf War Illness, Ratanesh K. Seth, Rabia Maqsood, Ayan Mondal, Dipro Bose, Diana Kimono, Larinda A. Holland, Patricia Janulewicz Lloyd, Nancy Klimas, Ronnie Horner, Kimberly Sullivan, Efrem S. Lim, Saurabh Chatterjee

Faculty Publications

Gulf War illness (GWI) is characterized by the persistence of inflammatory bowel disease, chronic fatigue, neuroinflammation, headache, cognitive impairment, and other medically unexplained conditions. Results using a murine model show that enteric viral populations especially bacteriophages were altered in GWI. The increased viral richness and alpha diversity correlated positively with gut bacterial dysbiosis and proinflammatory cytokines. Altered virome signature in GWI mice also had a concomitant weakening of intestinal epithelial tight junctions with a significant increase in Claudin-2 protein expression and decrease in ZO1 and Occludin mRNA expression. The altered virome signature in GWI, decreased tight junction protein level was …

Current Understanding Of West Nile Virus Clinical Manifestations, Immune Responses, Neuroinvasion, And Immunotherapeutic Implications, Fengwei Bai, E. Ashley Thompson, Parminder J.S. Vig, A. Arturo Leis

Faculty Publications

West Nile virus (WNV) is the most common mosquito-borne virus in North America. WNV-associated neuroinvasive disease affects all ages, although elderly and immunocompromised individuals are particularly at risk. WNV neuroinvasive disease has killed over 2300 Americans since WNV entered into the United States in the New York City outbreak of 1999. Despite 20 years of intensive laboratory and clinical research, there are still no approved vaccines or antivirals available for human use. However, rapid progress has been made in both understanding the pathogenesis of WNV and treatment in clinical practices. This review summarizes our current understanding of WNV infection in …

Dysbiosis-Associated Enteric Glial Cell Immune-Activation And Redox Imbalance Modulate Tight Junction Protein Expression In Gulf War Illness Pathology, Diana Kimono, Sutapa Sarkar, Muayad Albadrani, Ratanesh K. Seth, Dipro Bose, Ayan Mondal, Yuxi Li, Amar N. Kar, Mitzi Nagarkatti, Prakash Nagarkatti, Kimberly Sullivan, Patricia Janulewicz, Stephen Lasley, Ronnie Horner, Nancy Klimas, Saurabh Chatterjee

Faculty Publications

About 14% of veterans who suffer from Gulf war illness (GWI) complain of some form of gastrointestinal disorder but with no significant markers of clinical pathology. Our previous studies have shown that exposure to GW chemicals resulted in altered microbiome which was associated with damage associated molecular pattern (DAMP) release followed by neuro and gastrointestinal inflammation with loss of gut barrier integrity. Enteric glial cells (EGC) are emerging as important regulators of the gastrointestinal tract and have been observed to change to a reactive phenotype in several functional gastrointestinal disorders such as IBS and IBD. This study is aimed at …

Parasitic Microbiome Project: Grand Challenges, Nolwenn M. Dheilly, Joaquín Martínez Martínez, Karyna Rosario, Paul J. Brindley, Raina N. Fichorava, Jonathan Z. Kaye, Kevin D. Kohl, Laura J. Knoll, Julius Lukeš, Susan L. Perkins, Robert Poulin, Lynn Schriml, Luke R. Thompson

Faculty Publications

No abstract provided.

The Gut-Microbiome In Gulf War Veterans: A Preliminary Report, Patricia A. Janulewicz, Ratanesh K. Seth, Jeffrey M. Carlson, Joy Ajama, Emily Quinn, Timothy Heeren, Nancy Klimas, Steven M. Lasley, Ronnie Horner, Kimberly Sullivan, Saurabh Chatterjee

Faculty Publications

Gulf War Illness (GWI) is a chronic multi-symptom disorder affecting the central nervous system (CNS), immune and gastrointestinal (GI) systems of Gulf War veterans (GWV). We assessed the relationships between GWI, GI symptoms, gut microbiome and inflammatory markers in GWV from the Boston Gulf War Illness Consortium (GWIC). Three groups of GWIC veterans were recruited in this pilot study; GWV without GWI and no gastrointestinal symptoms (controls), GWV with GWI and no gastrointestinal symptoms (GWI-GI), GWV with GWI who reported gastrointestinal symptoms (GW+GI). Here we report on a subset of the first thirteen stool samples analyzed. Results showed significantly different …

Hinge Region In Dna Packaging Terminase Pul15 Of Herpes Simplex Virus: A Potential Allosteric Target For Antiviral Drugs, Lana F. Thaljeh, J. Ainsley Rothschild, Misagh Naderi, Lyndon M. Coghill

Faculty Publications

Approximately 80% of adults are infected with a member of the herpesviridae family. Herpesviruses establish life-long latent infections within neurons, which may reactivate into lytic infections due to stress or immune suppression. There are nine human herpesviruses (HHV) posing health concerns from benign conditions to life threatening encephalitis, including cancers associated with viral infections. The current treatment options for most HHV conditions mainly include several nucleoside and nucleotide analogs targeting viral DNA polymerase. Although these drugs help manage infections, their common mechanism of action may lead to the development of drug resistance, which is particularly devastating in immunocompromised patients. Therefore, …

Hinge Region In Dna Packaging Terminase Pul15 Of Herpes Simplex Virus: A Potential Allosteric Target For Antiviral Drugs, Lana F. Thaljeh, J. Ainsley Rothschild, Misagh Naderi, Lyndon M. Coghill, Jeremy M. Brown, Michal Brylinski

Faculty Publications

Approximately 80% of adults are infected with a member of the herpesviridae family. Herpesviruses establish life-long latent infections within neurons, which may reactivate into lytic infections due to stress or immune suppression. There are nine human herpesviruses (HHV) posing health concerns from benign conditions to life threatening encephalitis, including cancers associated with viral infections. The current treatment options for most HHV conditions mainly include several nucleoside and nucleotide analogs targeting viral DNA polymerase. Although these drugs help manage infections, their common mechanism of action may lead to the development of drug resistance, which is particularly devastating in immunocompromised patients. Therefore, …

Visualizing Nutrient Effects On Root Pattern Formation, Robert E. Zdor

Faculty Publications

This lab gives students hands-on experience with visualizing the root architecture of plants exposed to varying concentrations of the vital nutrient phosphorus. By maintaining Brassica sp. seedlings in the presence of different quantities of phosphate, students can quantify changes in the number of lateral roots as an example of how the environment influences plant pattern formation. Additional variables in the experimental design, such as the use of plant mutants altered in plant regulator action or the presence of plant regulators in the plant growth medium, allow for exploration of how plant growth regulators are involved in root development. The quantitative …

Rompi-Cdsa: Ring-Opening Metathesis Polymerization-Induced Crystallization-Driven Self-Assembly Of Metallo-Block Copolymers, Ye Sha, Md Anisur Rahman, Tianyu Zhu, Yujin Cha, C Wayne Mcalister, Chuanbing Tang

Faculty Publications

Polymerization-induced self-assembly (PISA) and crystallization-driven self-assembly (CDSA) are among the most prevailing methods for block copolymer self-assembly. Taking the merits of scalability of PISA and dimension control of CDSA, we report one-pot synchronous PISA and CDSA ring-opening metathesis polymerization (ROMP) to prepare nano-objects based on a crystalline poly(ruthenocene) motif. We denote this self-assembly methodology as ROMPI-CDSA to enable a simple, yet robust approach for the preparation of functional nanomaterials.

Multilevel Analysis In Rural Cancer Control: A Conceptual Framework And Methodological Implications, Tine Verreet, Cory J. Weaver, Hiromu Hino, Masahiko Hibi, Fabienne E. Poulain

Faculty Publications

Mitochondria are abundantly detected at the growth cone, the dynamic distal tip of developing axons that directs growth and guidance. It is, however, poorly understood how mitochondrial dynamics relate to growth cone behavior in vivo, and which mechanisms are responsible for anchoring mitochondria at the growth cone during axon pathfinding. Here, we show that in retinal axons elongating along the optic tract in zebrafish, mitochondria accumulate in the central area of the growth cone and are occasionally observed in filopodia extending from the growth cone periphery. Mitochondrial behavior at the growth cone in vivo is dynamic, with mitochondrial positioning …

Fisetin, A 3,7,3 ',4 '-Tetrahydroxyflavone Inhibits The Pi3k/Akt/Mtor And Mapk Pathways And Ameliorates Psoriasis Pathology In 2d And 3d Organotypic Human Inflammatory Skin Models, Konstantin G. Kousoulas

Faculty Publications

Psoriasis is a chronic immune-mediated skin disease that involves the interaction of immune and skin cells, and is characterized by cytokine-driven epidermal hyperplasia, deviant differentiation, inflammation, and angiogenesis. Because the available treatments for psoriasis have significant limitations, dietary products are potential natural sources of therapeutic molecules, which can repair the molecular defects associated with psoriasis and could possibly be developed for its management. Fisetin (3,7,3 ',4 '-tetrahydroxyflavone), a phytochemical naturally found in pigmented fruits and vegetables, has demonstrated proapoptotic and antioxidant effects in several malignancies. This study utilized biochemical, cellular, pharmacological, and tissue engineering tools to characterize the effects of …

Late Pleistocene Range Expansion Of North American Topminnows Accompanied By Admixture And Introgression, David D. Duvernell, Eric Westhafer, Jacob F. Schaefer

Faculty Publications

Aim: We used genome‐scale sampling to assess the phylogeography of a group of topminnows in the Fundulus notatus species complex. Two of the species have undergone extensive range expansions resulting in broadly overlapping distributions, and sympatry within drainages has provided opportunities for hybridization and introgression. We assessed the timing and pattern of range expansion in the context of late Pleistocene–Holocene drainage events and evaluated the evidence for introgressive hybridization between species.

Location: Central and southern United States including drainages of the Gulf of Mexico Coastal Plain and portions of the Mississippi River drainage in and around the Central …

Allopatric Speciation In Asia Contributed To The Diversity Anomaly Between Eastern Asia And Eastern North America: Evidence From Anchored Phylogenomics Of Stewartia (Theaceae), Jianhua Li, Peter Del Tredici, Alan R. Lemmon, Emily Moriarty Lemmon, Yunpeng Zhao, Chengxin Fu

Faculty Publications

Premise of research. The disjunct distribution of plant genera between eastern Asia (EA) and eastern North America (ENA) has long attracted the attention of biologists and biogeographers. For most genera that have been studied, there are more species in EA than in ENA, and the diversity anomaly may have resulted from the greater physiographical heterogeneity in EA than in ENA in conjunction with climate and sea level changes. However, few empirical studies have explicitly tested the association between species diversity and allopatric speciation events. The genus Stewartia (Theaceae) displays this diversity anomaly, with two species in ENA and 21 …

Food Consumption Patterns And Body Composition In Children: Moderating Effects Of Prop Taster Status, Lee Stoner, Nicholas Castro, Anna Kucharska-Newton, Abbie E. Smith-Ryan, Sally Lark, Michelle A. Williams, James Faulkner, Paula Skidmore

Faculty Publications

This cross-sectional study determined whether 6-n-propylthiouracil (PROP) taster status moderates the relationship between food consumption patterns and body composition in children. Children were recruited (n = 342, 50% female, 8–10 y) from across New Zealand. Using a food frequency questionnaire, these food consumption patterns were derived: Processed Foods, Fruit and Vegetables, and Breakfast Foods. Body composition variables included: body fat (%), fat mass (kg), fat mass index (FMI, kg/m²), body mass index (kg/m²) and waist to height ratio (W:Ht). Following adjustment for confounders, Processed Foods were positively associated with %fat (p = 0.015), fat mass (p …

Evaluation Of The Stock Structure Of Cobia (Rachycentron Canadum) In The Southeastern United States By Using Dart-Tag And Genetics Data, Matt Perkinson, Tanya Darden, Maggie Jamison, Matt J. Walker, Michael R. Denson, James Franks, Read Hendon, Susanna Musick, Eric S. Orbensen

Faculty Publications

Available tag-recapture and population genetics data for cobia (Rachycentron canadum) in the southeastern United States were evaluated to provide information on population structure and determine the geographic boundary between stocks in the Gulf of Mexico and the Atlantic Ocean. The movements of 1750 cobia were evaluated on the basis of assigned tagging and recapture zones. Genetic samples from an additional 2796 cobia collected during the presumed spawning season were genotyped at 10 microsatellite loci, and standard population genetic statistical analyses were applied to the resulting sample data set. Tag-recapture results indicate that cobia tagged south of Cape Canaveral, …