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Full-Text Articles in Life Sciences

Exploring The Intersection Between West Virginia Farmland Protection Boards, Landowners, And The West Virginia Agrarian Commons, Samuel W. Bayne, Joshua Lohnes Apr 2024

Exploring The Intersection Between West Virginia Farmland Protection Boards, Landowners, And The West Virginia Agrarian Commons, Samuel W. Bayne, Joshua Lohnes

Undergraduate Scholarship

New Roots Community Farm (NRCF) came to life through the purchase of a piece of land by the Fayette County Farmland Protection Board in a buy-protect-sell model. NRCF then partnered with The Agrarian Land Trust, a New England based organization experimenting with land commons models across the United States, to purchase the property and create the West Virginia Agrarian Commons (WVAC), an entity that could advance land access for the next generation farmers in WV. The experience of NRCF and the WVAC and the vision for its operation and extension of the buy-protect-sell model in partnership with county farmland protection …


Examining Eastern Food Hub's Food Access Programs: A Research Study Sustainable Development Internship (Sdi) Spring 2024, Devin Anthony Barkefelt, Fritz Boettner Apr 2024

Examining Eastern Food Hub's Food Access Programs: A Research Study Sustainable Development Internship (Sdi) Spring 2024, Devin Anthony Barkefelt, Fritz Boettner

Undergraduate Scholarship

In 2020, the US Department of Agriculture (USDA) introduced the Farmers to Families Food Box (FFFB) program. Its objectives were threefold: (1) deliver food to those in need, (2) support small and mid-sized producers, and (3) bolster food distribution companies through the pandemic. While the program ended in 2021, the USDA has continued to fund other programs, such as the Local Food Purchase Assistance Cooperative Agreement Program (LFPA) and the Local Food For Schools Cooperative Agreement Program (LFS). The WVU Center for Resilient Communities (CRC) is researched how previous, current, and future programs can be implemented to best support producers, …


Economic Comparison Of The Relative Costs And Efficiency Of Using Thermo Fisher Scientific Rapidhit™ Id System Versus Traditional Dna Laboratory Analysis, Paul J. Speaker Jan 2023

Economic Comparison Of The Relative Costs And Efficiency Of Using Thermo Fisher Scientific Rapidhit™ Id System Versus Traditional Dna Laboratory Analysis, Paul J. Speaker

Faculty & Staff Scholarship

With the emergence of any new technology, economic questions arise regarding the efficacy of the new technology in comparison to existing analytical techniques. While the question of ultimate interest concerns the return on investment (ROI) of the new technology, that ROI requires both an evaluation of the net benefits of the technology and an examination of the costs to implement the technology. In this report, we examine the full-loaded cost structure of traditional DNA analysis using fiscal year 2021 data from Project FORESIGHT and compare the cost per sample with the price structure of the Thermo Fischer Scientific RapidHit technology. …


Revisiting The Dual Pathway Hypothesis Of Chorismate Production In Plants, Joseph H. Lynch Mar 2022

Revisiting The Dual Pathway Hypothesis Of Chorismate Production In Plants, Joseph H. Lynch

Faculty & Staff Scholarship

The shikimate pathway, the seven enzymatic steps that synthesize chorismate from phosphoenolpyruvate and erythrose 4-phosphate, produces the last common precursor of the three aromatic amino acids. It is firmly established that all seven enzymes are present in plastids, and it is generally accepted that this organelle is likely the sole location for production of chorismate in plants. However, recently a growing body of evidence has provided support for a previous proposal that at least portions of the pathway are duplicated in the cytosol, referred to as the Dual Pathway Hypothesis. Here I revisit this obscure hypothesis by reviewing the findings …


Using Multiple Taxa And Wetland Classification Schemes For Enhanced Detection Of Biological Response Signatures To Human Impairment, Walter Veselka Iv, Walter S. Kordek, James T. Anderson Nov 2021

Using Multiple Taxa And Wetland Classification Schemes For Enhanced Detection Of Biological Response Signatures To Human Impairment, Walter Veselka Iv, Walter S. Kordek, James T. Anderson

Faculty & Staff Scholarship

Wetland indices of biological integrity (IBIs) are a common component in monitoring the wetland water resources as required by the United States’ Clean Water Act (CWA). The effectiveness of an IBI to monitor disturbance is dependent on the metrics being consistently responsive to measures of human disturbance within a described classification category. We present IBIs designed for two types of commonly used wetland classification systems – the hydrogeomorphic (HGM) and the National Wetlands Inventory (NWI). The metrics making up the IBIs were derived from anuran, avian, macroinvertebrate, and vegetation communities; each representing increasing levels of resources associated with gathering the …


Bacilli As Sources Of Agrobiotechnology: Recent Advances And Future Directions, Zerihun T. Dame, Mahfuz Rahman, Tofazzal Islam Jan 2021

Bacilli As Sources Of Agrobiotechnology: Recent Advances And Future Directions, Zerihun T. Dame, Mahfuz Rahman, Tofazzal Islam

Faculty & Staff Scholarship

The group bacilli represent the Gram-positive ubiquitous spore-forming bacteria. Their diversity, versatility and the ability of producing diverse secondary metabolites including enzymes created enormous potential for applications in agriculture, biotechnology, environment and medicine. The bacilli are considered as one of the most studied groups of bacteria providing plant growth-promotion and biocontrol of multiple diseases reflecting their vital role in enhancing plants’ tolerance to biotic and abiotic stresses. Some of the Bacillus species are available commercially as phytostimulants, biopesticides, and biofertilizers. Genetically engineered plants such as maize, cotton, brinjal with endotoxins producing genes from Bacillus thuringiensis (Bt) has revolutionized agriculture. Many …


Meta-Analyses Support A Taxonomic Model For Representations Of Different Categories Of Audio-Visual Interaction Events In The Human Brain, Matt Csonka, Nadia Mardmomen, Paula J. Webster, Julie A. Brefczynski-Lewis, Chris Frum, James W. Lewis Jan 2021

Meta-Analyses Support A Taxonomic Model For Representations Of Different Categories Of Audio-Visual Interaction Events In The Human Brain, Matt Csonka, Nadia Mardmomen, Paula J. Webster, Julie A. Brefczynski-Lewis, Chris Frum, James W. Lewis

Faculty & Staff Scholarship

Our ability to perceive meaningful action events involving objects, people and other animate agents is characterized in part by an interplay of visual and auditory sensory processing and their cross-modal interactions. However, this multisensory ability can be altered or dysfunctional in some hearing and sighted individuals, and in some clinical populations. The present meta-analysis sought to test current hypotheses regarding neurobiological architectures that may mediate audio-visual multisensory processing. Reported coordinates from 82 neuroimaging studies (137 experiments) that revealed some form of audio-visual interaction in discrete brain regions were compiled, converted to a common coordinate space, and then organized along specific …


Fertilization And Tree Species Influence On Stable Aggregates In Forest Soil, Jacob E. Kemner, Mary Beth Adams, Louis M. Mcdonald, William T. Peterjohn, Charlene N. Kelly Jan 2021

Fertilization And Tree Species Influence On Stable Aggregates In Forest Soil, Jacob E. Kemner, Mary Beth Adams, Louis M. Mcdonald, William T. Peterjohn, Charlene N. Kelly

Faculty & Staff Scholarship

Abstract: Background and objectives: aggregation and structure play key roles in the water-holding capacity and stability of soils and are important for the physical protection and storage of soil carbon (C). Forest soils are an important sink of ecosystem C, though the capacity to store C may be disrupted by the elevated atmospheric deposition of nitrogen (N) and sulfur (S) compounds by dispersion of soil aggregates via acidification or altered microbial activity. Furthermore, dominant tree species and the lability of litter they produce can influence aggregation processes. Materials and methods: we measured water-stable aggregate size distribution and aggregate-associated organic matter …


Nitrogen Fertilization, Stand Age, And Overstory Tree Species Impact The Herbaceous Layer In A Central Appalachian Hardwood Forest, Lacey J. Smith, Kirsten Stephan Jan 2021

Nitrogen Fertilization, Stand Age, And Overstory Tree Species Impact The Herbaceous Layer In A Central Appalachian Hardwood Forest, Lacey J. Smith, Kirsten Stephan

Faculty & Staff Scholarship

Research Highlights: Herb-layer community composition, abundance, species richness, and Shannon–Wiener diversity index are shaped by nitrogen fertilization, disturbance history, and the overstory tree species in its immediate vicinity. Background and Objectives: While the herbaceous layer in deciduous forests is increasingly recognized for its importance in various aspects of forest ecosystem function, this study sought to describe the factors impacting the herbaceous layer. Specifically, this study’s objective was to quantify and compare herb-layer species composition, cover, and other community indices in watersheds with (a) different levels of N deposition, (b) different stand ages due to differing disturbance histories, and (c) different …


Biocontrol Agent, Biofumigation, And Grafting With Resistant Rootstock Suppress Soil-Borne Disease And Improve Yield Of Tomato In West Virginia, Mahfuz Rahman, Tofazzal Islam, Lewis Jett, James Kotcon Jan 2021

Biocontrol Agent, Biofumigation, And Grafting With Resistant Rootstock Suppress Soil-Borne Disease And Improve Yield Of Tomato In West Virginia, Mahfuz Rahman, Tofazzal Islam, Lewis Jett, James Kotcon

Faculty & Staff Scholarship

Organic tomato growers in West Virginia and neighboring states suffer serious economic losses each year due to soil-borne wilt diseases caused by fungal pathogens including Verticillium dahliae. This study determined the efficacies of biological control agents (BCAs – Serenade SOIL and Prestop), bio-fumigants and transplants grafted to a resistant rootstock in suppressing wilt disease in heirloom tomato cv. Mortgage Lifter in a certified organic production system in West Virginia in two consecutive years. Prestop and Serenade treatments resulted in higher seedling vigor at the early stage. However, within 40 days of field set in the fungal pathogen infested soil, grafted …


The Application Of Systems Science In Nutrition-Related Behaviors And Outcomes Implementation Research: A Scoping Review, Ayron E. Walker, Rachel A. Wattick, Melissa D. Olfert Jan 2021

The Application Of Systems Science In Nutrition-Related Behaviors And Outcomes Implementation Research: A Scoping Review, Ayron E. Walker, Rachel A. Wattick, Melissa D. Olfert

Faculty & Staff Scholarship

Use of systems science can improve the dissemination and implementation (D&I) process. However, little is known about use of systems science in nutrition D&I research. The purpose of this article is to synthesize the ways in which systems science methodology is applied in nutrition D&I research. Scoping review methodology involved searching 6 academic databases for full-text, peer-reviewed, English articles published between 1970 and 2020 that employed systems science within nutrition D&I research. Data extraction included intervention type, population, study aim, methods, theoretical approach, outcomes, and results. Descriptive statistics and qualitative thematic analysis followed. Thirty-four retained articles qualitatively identified benefits (successful …


Biofortification Of Sodium Selenate Improves Dietary Mineral Contents And Antioxidant Capacity Of Culinary Herb Microgreens, Rachel Newman G. Newman, Youyoun Moon, Carl E. Sams, Janet C. Tou, Nicole L. Waterland Jan 2021

Biofortification Of Sodium Selenate Improves Dietary Mineral Contents And Antioxidant Capacity Of Culinary Herb Microgreens, Rachel Newman G. Newman, Youyoun Moon, Carl E. Sams, Janet C. Tou, Nicole L. Waterland

Faculty & Staff Scholarship

Selenium biofortification of plants has been suggested as a method of enhancing dietary seleniumintake to prevent deficiency and chronic disease in humans, while avoiding toxic levels of intake. Popular herbs such as basil (Ocimum basilicum L.), cilantro (Coriandrum sativum L.), and scallions (Allium fistulosum L.) present an opportunity for biofortification as these plants are used for added flavors to meals and are available as microgreens, young plants with increasing popularity in the consumer marketplace. In this study, basil, cilantro, and scallion microgreens were biofortified with sodium selenate under hydroponic conditions at various selenium concentrations to investigate the effects on yield, …


Evaluation Of Calcium Application Methods On Delaying Plant Wilting Under Water Deficit In Bedding Plants, Suejin Park, Nicole L. Waterland Jan 2021

Evaluation Of Calcium Application Methods On Delaying Plant Wilting Under Water Deficit In Bedding Plants, Suejin Park, Nicole L. Waterland

Faculty & Staff Scholarship

Floriculture crops can lose their aesthetic quality due to water deficit during postproduction. Calcium is a secondary messenger in plant stress signaling, and the treatment of calcium has been proposed to alleviate damage by various abiotic stresses. The objective of this research was to evaluate application methods of calcium to delay plant wilting under water deficiency in three species of bedding plants: viola (Viola cornuta), impatiens (Impatiens walleriana), and petunia (Petunia grandiflora). Three application methods were compared including spray, drench, and pre-drench. Calcium was applied as CaCl2 and Ca(NO3)2 at three concentrations ranging from 50 to …


A Spatially Distributed Investigation Of Stream Water Temperature In A Contemporary Mixed-Land-Use Watershed, Jason P. Horne, Jason A. Hubbart Jul 2020

A Spatially Distributed Investigation Of Stream Water Temperature In A Contemporary Mixed-Land-Use Watershed, Jason P. Horne, Jason A. Hubbart

Faculty & Staff Scholarship

Stream water temperature (◦C) is an important physical variable that influences many biological and abiotic water quality processes. The intermingled mosaic of land-use/land-cover (LULC) types and corresponding variability in stream water temperature (Tw) processes in contemporary mixed-land-use watersheds necessitate research to advance management and policy decisions. Water temperature was analyzed from 21 gauging sites using a nested-scale experimental watershed study design. Results showed that forested land use was negatively correlated (α = 0.05) with mean and maximum Tw. Agricultural land use was significantly positively correlated (α = 0.05) with maximum Tw except during the spring season. Mixed development and Tw …


Treatment With Calcium Chloride Enhances Water Deficit Stress Tolerance In Viola (Viola Cornuta), Suejin Park, Youyoun Moon, Nicole L. Waterland Jan 2020

Treatment With Calcium Chloride Enhances Water Deficit Stress Tolerance In Viola (Viola Cornuta), Suejin Park, Youyoun Moon, Nicole L. Waterland

Faculty & Staff Scholarship

Water deficit stress can reduce the postproduction shelf life and marketability of floriculture crops. To alleviate the damage by water deficiency, plants need to limit transpirational water loss by inducing stomatal closure. Osmotic stress induces stomatal closure like the response to water deficit stress. It could be used as a convenient tool to enhance water deficit stress tolerance by reducing water loss. The objective of this research was to investigate whether osmotic treatment with a high concentration of chemical solutions could trigger a response to osmotic stress so that stomatal closure can be induced, resulting in enhanced water deficit stress …


The Rise To Dominance Of Genetic Model Organisms And The Decline Of Curiosity-Driven Organismal Research, Sarah M. Farris Jan 2020

The Rise To Dominance Of Genetic Model Organisms And The Decline Of Curiosity-Driven Organismal Research, Sarah M. Farris

Faculty & Staff Scholarship

Curiosity-driven, basic biological research “…performed without thought of practical ends…” establishes fundamental conceptual frameworks for future technological and medical breakthroughs. Traditionally, curiosity-driven research in biological sciences has utilized experimental organisms chosen for their tractability and suitability for studying the question of interest. This approach leverages the diversity of life to uncover working solutions (adaptations) to problems encountered by living things, and evolutionary context as to the extent to which these solutions may be generalized to other species. Despite the well-documented success of this approach, funding portfolios of United States granting agencies are increasingly filled with studies on a few species …


What Might It Cost To Increase Soil Organic Carbon Using No-Till On U.S. Cropland?, Mark Sperow Jan 2020

What Might It Cost To Increase Soil Organic Carbon Using No-Till On U.S. Cropland?, Mark Sperow

Faculty & Staff Scholarship

Existing research provides estimates of the biophysical potential for increasing soil organic carbon (SOC) stock, however additional research is needed to enhance our understanding of the economic potential for agricultural soils to offset or help reduce CO2emissions. This study derives the marginal cost to increase SOC sequestration by combining SOC sequestration potential estimates developed using the Intergovernmental Panel on Climate Change (IPCC) factors with an existing payment scheme that was designed to increase no-till (NT) adoption on U.S. cropland. The marginal costs of increasing SOC is a function of the amount of SOC that could be increased through NT and …


Experiences Of Multidisciplinary Health Professionals From A Culinary Medicine Cultural Immersion: Qualitative Analysis, Melissa D. Olfert, Rachel A. Wattick, Rebecca L. Hagedorn Jan 2020

Experiences Of Multidisciplinary Health Professionals From A Culinary Medicine Cultural Immersion: Qualitative Analysis, Melissa D. Olfert, Rachel A. Wattick, Rebecca L. Hagedorn

Faculty & Staff Scholarship

Purpose: Increasing Culinary Health Opportunities for Professionals (iCHOP) aimed to educate future and current health pro- fessionals on nutrition as medicine. Methods: Two cohorts each participated in a 16-week course followed by a cultural immersion in Tuscany, Italy. The course took place online through West Virginia University. After the course, participants traveled to Tuscany, Italy for a 2-week cultural im- mersion. The online course covered culinary medicine, the Mediterranean Diet, and cultural comparisons. The cultural immersion consisted of hands-on activities including culinary lessons, food production and organic farm tours, and tastings of Mediterranean foods. Data was collected through personal journaling …


Eb4cast Approach Improves Science Communication With Stakeholders In A College-Based Health Program, Melissa D. Olfert, Makenzie L. Barr, Rebecca L. Hagedorn, Rachel A. Wattick, Wenjun Zhou, Tanya M. Horacek, Anne E. Mathews, Kendra K. Kattelmann, Tandalayo Kidd, Adrienne A. White, Onikia N. Brown, Jesse Stabile Morrell, Lisa Franzen-Castle, Karla P. Shelnutt, Carol Byrd-Bredbenner, Terezie Tolar-Peterson, Geoffrey W. Greene, Sarah E. Colby Jan 2020

Eb4cast Approach Improves Science Communication With Stakeholders In A College-Based Health Program, Melissa D. Olfert, Makenzie L. Barr, Rebecca L. Hagedorn, Rachel A. Wattick, Wenjun Zhou, Tanya M. Horacek, Anne E. Mathews, Kendra K. Kattelmann, Tandalayo Kidd, Adrienne A. White, Onikia N. Brown, Jesse Stabile Morrell, Lisa Franzen-Castle, Karla P. Shelnutt, Carol Byrd-Bredbenner, Terezie Tolar-Peterson, Geoffrey W. Greene, Sarah E. Colby

Faculty & Staff Scholarship

Communicating scientific results with community partners is often lacking in intervention programs, thus eB4CAST was developed to facilitate impact sharing. This article investigated using the eB4CAST dissemination tool to communicate impact from a campus-based obesity prevention program. Data from Get Fruved RCT university sites collected at baseline were used to generate eB4CAST reports. Experts (n = 13) and RCT sites (n = 15) were asked to provide feedback on eB4CAST reports based on appeal, understanding, and clarity. On all Likert items, participants rated above 7 on each (out of 10). Positive responses from open-ended questions included eB4CAST reports being clear, …


Mechanism Of Plant Growth Promotion And Disease Suppression By Chitosan Biopolymer, Moutoshi Chakraborty, Mirza Hasanuzzaman, Mahfuzur Rahman, Md. Arifur Rahman Khan, Pankaj Bhowmik, Nur Uddin Mahmud, Mohsin Tanveer, Tofazzal Islam Jan 2020

Mechanism Of Plant Growth Promotion And Disease Suppression By Chitosan Biopolymer, Moutoshi Chakraborty, Mirza Hasanuzzaman, Mahfuzur Rahman, Md. Arifur Rahman Khan, Pankaj Bhowmik, Nur Uddin Mahmud, Mohsin Tanveer, Tofazzal Islam

Faculty & Staff Scholarship

The chitosan (CHT) biopolymer is a de-acetylated chitin derivative that exists in the outer shell of shrimp, shellfish, lobster or crabs, as well as fungal cell walls. Because of its biodegradability, environmental non-toxicity, and biocompatibility, it is an ideal resource for sustainable agriculture. The CHT emerged as a promising agent used as a plant growth promoter and also as an antimicrobial agent. It induces plant growth by influencing plant physiological processes like nutrient uptake, cell division, cell elongation, enzymatic activation and synthesis of protein that can eventually lead to increased yield. It also acts as a catalyst to inhibit the …


Wheat Blast: A New Threat To Food Security, M. Tofazzal Islam, Dipali Rani Gupta, Akbar Hossain, Krishna K. Roy, Xinyao He, Muhammad R. Kabir, Pawan K. Singh, Md. Arifur Rahman Khan, Mahfuzur Rahman, Guo-Liang Wang Jan 2020

Wheat Blast: A New Threat To Food Security, M. Tofazzal Islam, Dipali Rani Gupta, Akbar Hossain, Krishna K. Roy, Xinyao He, Muhammad R. Kabir, Pawan K. Singh, Md. Arifur Rahman Khan, Mahfuzur Rahman, Guo-Liang Wang

Faculty & Staff Scholarship

Wheat blast, caused by the Magnaporthe oryzae Triticum (MoT) lineage (synonym Pyricularia oryzae Triticum lineage), is a destructive disease in South America and Bangladesh. It is primarily a disease of wheat head, which can cause yield loss up to 100% under favorable disease conditions. The head infection results in complete or partial bleaching of the spike above the point of infection with either no grain or shriveled grain with low test weight. Due to low fungicide efficacy against the disease and lack of availability of resistant varieties, an integrated management program should be adopted to control this serious wheat disease. …


Suitable Methods For Isolation, Culture, Storage And Identification Of Wheat Blast Fungus Magnaporthe Oryzae Triticum Pathotype, Dipali Rani Gupta, Musrat Zahan Surovy, Nur Uddin Mahmud, Moutoshi Chakraborty, Sanjoy Kumar Paul, Md. Shaid Hossain, Pallab Bhattacharjee, Md. Shabab Mehebub, Kanistha Rani, Rumana Yeasmin, Mahfuzur Rahman, Md Tofazzal Islam Jan 2020

Suitable Methods For Isolation, Culture, Storage And Identification Of Wheat Blast Fungus Magnaporthe Oryzae Triticum Pathotype, Dipali Rani Gupta, Musrat Zahan Surovy, Nur Uddin Mahmud, Moutoshi Chakraborty, Sanjoy Kumar Paul, Md. Shaid Hossain, Pallab Bhattacharjee, Md. Shabab Mehebub, Kanistha Rani, Rumana Yeasmin, Mahfuzur Rahman, Md Tofazzal Islam

Faculty & Staff Scholarship

Wheat blast disease caused by a South American lineage of Magnaporthe oryzae Triticum (MoT) pathotype has emerged as a serious threat to wheat production in Bangladesh since its first emergence in 2016. Efficient and suitable methods for isolation, storage, inoculum production and molecular characterization of the pathogen can help in achieving the target of sustainable management of the disease in a relatively short period of time. In this study, we aimed to develop suitable methods for isolation, storage and morphological characterization and molecular identification of MoT isolates collected from the blast-infected wheat fields in Bangladesh. This process included modification of …


Vulnerability Of High-Elevation Endemic Salamanders To Climate Change: A Case Study With The Cow Knob Salamander (Plethodon Punctatus), Carl D. Jacobsen, Donald J. Brown, William D. Flint, Thomas K. Pauley, Kurt A. Buhlmann, Joseph C. Mitchell Jan 2020

Vulnerability Of High-Elevation Endemic Salamanders To Climate Change: A Case Study With The Cow Knob Salamander (Plethodon Punctatus), Carl D. Jacobsen, Donald J. Brown, William D. Flint, Thomas K. Pauley, Kurt A. Buhlmann, Joseph C. Mitchell

Faculty & Staff Scholarship

Rapid contemporary climate change is a potential threat to long-term persistence of montane wildlife species because they often have narrow thermal tolerances and have limited potential to shift their distributions. The Appalachian Mountain region in the eastern United States is a global biodiversity hotspot for woodland salamanders (genus Plethodon), many of which are high-elevation endemic species. Robust assessments of the vulnerability of high-elevation endemic salamanders to climate change, including delineation of future potential climate refugia, are needed to guide climate change adaptations strategies. The Cow Knob Salamander (Plethodon punctatus) is a species of conservation concern found at …


A Comparison Of Wetland Characteristics Between Agricultural Conservation Easement Program And Public Lands Wetlands In West Virginia, Usa, Katherine E. Lewis, Christopher T. Rota, James T. Anderson Jan 2020

A Comparison Of Wetland Characteristics Between Agricultural Conservation Easement Program And Public Lands Wetlands In West Virginia, Usa, Katherine E. Lewis, Christopher T. Rota, James T. Anderson

Faculty & Staff Scholarship

In West Virginia, USA, there are 24 conservation easement program wetlands enrolled in the Agricultural Conservation Easement Program (ACEP). These wetlands are located on private agricultural land and are passively managed. Due to their location within fragmented agricultural areas, wetlands enrolled in ACEP in West Virginia have the potential to add wetland ecosystem services in areas that are lacking these features. We evaluated ACEP wetlands compared to reference wetlands on public land in West Virginia by using surrounding land cover, vegetative cover, and wetland features and stressors such as the presence or absence of erosion, upland inclusion, algal mats, and …


Saccharomyces Arboricola And Its Hybrids’ Propensity For Sake Production: Interspecific Hybrids Reveal Increased Fermentation Abilities And A Mosaic Metabolic Profile, Matthew J. Winans, Yuki Yamamoto, Yuki Fujimaru, Yuki Kusaba, Jennifer E G Gallagher, Hiroshi Kitagaki Jan 2020

Saccharomyces Arboricola And Its Hybrids’ Propensity For Sake Production: Interspecific Hybrids Reveal Increased Fermentation Abilities And A Mosaic Metabolic Profile, Matthew J. Winans, Yuki Yamamoto, Yuki Fujimaru, Yuki Kusaba, Jennifer E G Gallagher, Hiroshi Kitagaki

Faculty & Staff Scholarship

The use of interspecific hybrids during the industrial fermentation process has been well established, positioning the frontier of advancement in brewing to capitalize on the potential of Saccharomyces hybridization. Interspecific yeast hybrids used in modern monoculture inoculations benefit from a wide range of volatile metabolites that broaden the organoleptic complexity. This is the first report of sake brewing by Saccharomyces arboricola and its hybrids. S. arboricola x S. cerevisiae direct-mating generated cryotolerant interspecific hybrids which increased yields of ethanol and ethyl hexanoate compared to parental strains, important flavor attributes of fine Japanese ginjo sake rice wine. Hierarchical clustering heatmapping with …


Blood Substitution Therapy Rescues The Brain Of Mice From Ischemic Damage, Xuefang Ren, Heng Hu, Imran Farooqi, James W. Simpkins Jan 2020

Blood Substitution Therapy Rescues The Brain Of Mice From Ischemic Damage, Xuefang Ren, Heng Hu, Imran Farooqi, James W. Simpkins

Faculty & Staff Scholarship

Acute stroke causes complex, pathological, and systemic responses that have not been treatable by any single medication. In this study, using a murine transient middle cerebral artery occlusion stroke model, a novel therapeutic strategy is proposed, where blood replacement (BR) robustly reduces infarctions and improves neurological deficits in mice. Our analyses of immune cell subsets suggest that BR therapy substantially decreases neutrophils in blood following a stroke. Electrochemiluminescence detection demonstrates that BR therapy reduces cytokine storm in plasma and ELISA demonstrates reduced levels of matrix metalloproteinase-9 (MMP-9) in the plasma and brains at different time points post-stroke. Further, we have …


Oxidative Stress Responses And Nutrient Starvation In Mchm Treated Saccharomyces Cerevisiae, Michael C. Ayers, Zachary N. Sherman, Jennifer E. G. Gallagher Jan 2020

Oxidative Stress Responses And Nutrient Starvation In Mchm Treated Saccharomyces Cerevisiae, Michael C. Ayers, Zachary N. Sherman, Jennifer E. G. Gallagher

Faculty & Staff Scholarship

In 2014, the coal cleaning chemical 4-methylcyclohexane methanol (MCHM) spilled into the water supply for 300,000 West Virginians. Initial toxicology tests showed relatively mild results, but the underlying effects on cellular biology were underexplored. Treated wildtype yeast cells grew poorly, but there was only a small decrease in cell viability. Cell cycle analysis revealed an absence of cells in S phase within thirty minutes of treatment. Cells accumulated in G1 over a six-hour time course, indicating arrest instead of death. A genetic screen of the haploid knockout collection revealed 329 high confidence genes required for optimal growth in MCHM. These …


Associations Of Adiposity And Diet Quality With Serum Ceramides In Middle-Aged Adults With Cardiovascular Risk Factors, Margaret A. Drazba, Ida Holaskova, Nadine R. Sahyoun, Melissa Ventura Marra May 2019

Associations Of Adiposity And Diet Quality With Serum Ceramides In Middle-Aged Adults With Cardiovascular Risk Factors, Margaret A. Drazba, Ida Holaskova, Nadine R. Sahyoun, Melissa Ventura Marra

Faculty & Staff Scholarship

Rates of adverse cardiovascular events have increased among middle-aged adults. Elevated ceramides have been proposed as a risk factor for cardiovascular events. Diet quality and weight status are inversely associated with several traditional risk factors; however, the relationship to ceramides is less clear. This study aimed to determine associations of adiposity and diet quality with circulating ceramides in middle-aged adults (n = 96). Diet quality was estimated using the Healthy Eating Index 2015 (HEI-2015). Serum ceramide concentrations were determined by liquid chromatography–mass spectrometry. A ceramide risk score was determined based on ceramides C16:0, C18:0, and C24:1 and their ratios to …


Cooking And Meal Planning As Predictors Of Fruit And Vegetable Intake And Bmi In First-Year College Students, Andrea J. Hanson, Kendra K. Kattleman, Lacey A. Mccormack, Wenjun Zhou, Onikia N. Brown, Tanya M. Horacek, Karla P. Shelnutt, Tandalayo Kidd, Audrey Opoku-Acheampong, Lisa D. Franzen-Castle, Melissa D. Olfert, Sarah E. Colby Jan 2019

Cooking And Meal Planning As Predictors Of Fruit And Vegetable Intake And Bmi In First-Year College Students, Andrea J. Hanson, Kendra K. Kattleman, Lacey A. Mccormack, Wenjun Zhou, Onikia N. Brown, Tanya M. Horacek, Karla P. Shelnutt, Tandalayo Kidd, Audrey Opoku-Acheampong, Lisa D. Franzen-Castle, Melissa D. Olfert, Sarah E. Colby

Faculty & Staff Scholarship

The objective was to determine if cooking skills and meal planning behaviors are associated with greater fruit and vegetable intake and lower body mass index (BMI) in first-year college students who are at risk for excessive weight gain. A cross-sectional analysis was conducted using baseline data from a multi-state research project aimed at preventing weight gain in first-year college students. Cooking type, frequency and confidence, self-instruction for healthful mealtime behavior intention, self-regulation of healthful mealtime behavior, and cup equivalents of fruits and vegetables (FV) were measured using validated surveys. BMI was calculated from measured height and weight. First-year students (n …


Neural Processing Of Communication Signals: The Extent Of Sender–Receiver Matching Varies Across Species Of Apteronotus, Kathryne M. Allen, Gary Marsat Jan 2019

Neural Processing Of Communication Signals: The Extent Of Sender–Receiver Matching Varies Across Species Of Apteronotus, Kathryne M. Allen, Gary Marsat

Faculty & Staff Scholarship

As communication signal properties change, through genetic drift or selective pressure, the sensory systems that receive these signals must also adapt to maintain sensitivity and adaptability in an array of contexts. Shedding light on this process helps us to understand how sensory codes are tailored to specific tasks. In a species of weakly electric fish, Apteronotus albifrons, we examined the unique neurophysiological properties that support the encoding of electrosensory communication signals that the animal encounters in social exchanges. We compare our findings to the known coding properties of the closely related species Apteronotus leptorhynchus to establish how these animals …