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Pseudomonas Aeruginosa And Epithelial Permeability: Role Of Virulence Factors Elastase And Exotoxin A., Ali Azghani
Pseudomonas Aeruginosa And Epithelial Permeability: Role Of Virulence Factors Elastase And Exotoxin A., Ali Azghani
Ali Azghani
Lung injury in bacterial infection is a multifactorial phenomenon that involves bacterial metabolites and host factors. Primary isolates of type II pneumocytes and established cultures of Madin-Darby canine kidney (MDCK) cells were used to study effects of Pseudomonas aeruginosa exoproducts on epithelial paracellular permeability. The results indicate that elastase (PE) and exotoxin A (Exo A) have different, but complementary, actions that diminish epithelial barrier function. We measured transepithelial electrical resistance (TER) and permeability coefficient for mannitol (Pm) across cell monolayers plated on tissue culture membranes. Application of 100 ng/ml of Exo A to the basal side decreased TER from 1,405 …