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Cocultivation Of Caprine Arthritis Encephalitis Virus-Infected Macrophages With Primarygoat Synovial Cells, Supachart Panneum, Wilairat Chumsing, Theera Rukkwamsuk
Cocultivation Of Caprine Arthritis Encephalitis Virus-Infected Macrophages With Primarygoat Synovial Cells, Supachart Panneum, Wilairat Chumsing, Theera Rukkwamsuk
Turkish Journal of Veterinary & Animal Sciences
Primary goat synovial cells were developed to be cocultivated with caprine arthritis encephalitis virus (CAEV)-infected monocytes. Two CAEV-seropositive goats and one CAEV-seronegative goat were confirmed for viral particles in the blood using the PCR method. Thereafter, heparinized blood samples from the three goats were collected and isolated for monocytes, which were further cultivated to differentiate into macrophages. Cultivated macrophages of 7-10 days old were cocultivated with the primary goat synovial cells for 7 days. Microscopic examination was performed every 2 days for multinucleated syncytial formation, and immunofluorescence assay was applied using monoclonal antibody against CAEV on day 7 to detect …