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Calving Assistance Influences The Occurrence Of Umbilical Cord Pathologies Treated Surgically In Calves, Carlos Moscuzza, Horacio Milicich, Guadalupe Alvarez, Betiana Gutierrez, Mariano Nahum Jan 2014

Calving Assistance Influences The Occurrence Of Umbilical Cord Pathologies Treated Surgically In Calves, Carlos Moscuzza, Horacio Milicich, Guadalupe Alvarez, Betiana Gutierrez, Mariano Nahum

Turkish Journal of Veterinary & Animal Sciences

Ninety-two umbilical pathologies were surgically treated in Holstein, Aberdeen Angus, and Polled Hereford calves, both males and females, whose ages ranged between 2 and 8 months. The pathology diagnosis was performed considering the main anatomical elements involved in the umbilical cord, the inflammation progression, and the macroscopic characteristics of injuries. The umbilical cord pathologies were related to calving characteristics. The proportion of affected umbilical cords in calves born via assisted calving (77%) was significantly higher than that observed in calves born from normal parturition. The umbilical cord pathology of highest occurrence was omphalophlebitis (53.2%). A considerable proportion of infectious diseases …


Evaluation Of Ultrasonography As A Diagnostic Tool For Hepatic Hydatid Cysts In Sheep, Hussein Awad Hussein, Mohammed Elrashidy Jan 2014

Evaluation Of Ultrasonography As A Diagnostic Tool For Hepatic Hydatid Cysts In Sheep, Hussein Awad Hussein, Mohammed Elrashidy

Turkish Journal of Veterinary & Animal Sciences

This retrospective study was designed to evaluate the use of ultrasonography for the diagnosis of hepatic hydatid cysts. This study was conducted on 22 sheep, which were classified into 2 groups according to their liver health group (healthy liver = 13 and cystic liver = 9). Biochemically, serum concentrations of gamma-glutamyl transferase, aspartate aminotransferase, total bilirubin, and globulins were significantly increased (P < 0.01) while albumin was lowered (P < 0.01) in sheep with cystic livers. Ultrasonographically, rounded, anechoic, and unilocular hydatid cysts with elliptical circumferences ranging from 6 to 10 cm were seen. The interiors of cysts contained echogenic particulate materials, septations, or fine echoes. At the 10th intercostal space (ICS), ventral margin, size, thickness, and angle of livers increased in sheep with hepatic cysts, while the diameter of portal vein decreased in sheep with liver cysts. At the 9th ICS, the circumference of the gall bladder was lowered in sheep with hepatic cysts (P < 0.01). The sensitivity, specificity, and positive and negative predictive values of ultrasonography for diagnosis of hepatic hydatid cysts were 80%, 100%, and 100% and 83%, respectively. In conclusion, ultrasonography alone or in combination with some biochemical parameters reflecting liver function could be helpful for the diagnosis of hepatic hydatid cysts in sheep.


Evaluation Of Ovsynch Protocols For Timed Artificial Insemination In Water Buffaloes In Bangladesh, Md Nazmul Hoque, Anup Kumar Talukder, Masuma Akter, Mohammed Shamsuddin Jan 2014

Evaluation Of Ovsynch Protocols For Timed Artificial Insemination In Water Buffaloes In Bangladesh, Md Nazmul Hoque, Anup Kumar Talukder, Masuma Akter, Mohammed Shamsuddin

Turkish Journal of Veterinary & Animal Sciences

A total of 65 water buffaloes (groups A, B, and C) at =>60 days postpartum with a body condition score (BCS) of =>2.5 were selected to evaluate ovsynch protocols for timed artificial insemination (TAI). The group A buffaloes (n = 25) were treated with a simple ovsynch protocol (GnRH - Day 7 - PGF2 alpha - Day 2 - GnRH -16 h - TAI). The group B buffaloes (n = 22) received PGF2 alpha treatment 12 days before the initiation of simple ovsynch (PGF2 alpha at Day -12 + simple ovsynch; modified ovsynch). The group C buffaloes (n …


The Effects Of Lh On Progesterone Production By Cell Subpopulations Isolated From Early And Late Luteal Phase Goat Corpora Lutea, Hakan Kalender, Şevket Arikan, Özkan Şi̇mşek Jan 2014

The Effects Of Lh On Progesterone Production By Cell Subpopulations Isolated From Early And Late Luteal Phase Goat Corpora Lutea, Hakan Kalender, Şevket Arikan, Özkan Şi̇mşek

Turkish Journal of Veterinary & Animal Sciences

The objective of this study was to investigate the complementary effects of luteinizing hormone (LH) on cell subpopulations. Corpora lutea were collected on days 5 and 15 of the cycle. The luteal tissues were treated with collagenase for cell dissociation. Percoll density-gradient centrifugation was performed to separate cells into 2 distinct subpopulations. Luteal cells (5 × 104 cells/well) were cultured with and without 22(R)-hydroxycholesterol (22R-HC, 10 µg/mL) and LH (10 and 100 ng/mL) alone, and also with 22R-HC (10 µg/mL) plus LH (10 and 100 ng/mL), in a serum-free culture medium for 6 days of incubation. Untreated cells isolated from …


Gallbladder Mucocele In A Dog: An Ultrasonography And Pathology Report, Marudhai Thangapandiyan, Ramasamy Sridhar, Kirubakaran Jeyaraja, Masilamani Sakthivelan, Thangaraj Mohanapriya, Natesan Pazhanivel Jan 2014

Gallbladder Mucocele In A Dog: An Ultrasonography And Pathology Report, Marudhai Thangapandiyan, Ramasamy Sridhar, Kirubakaran Jeyaraja, Masilamani Sakthivelan, Thangaraj Mohanapriya, Natesan Pazhanivel

Turkish Journal of Veterinary & Animal Sciences

A 9-year old male, German shepherd dog was presented to the Madras Veterinary College Teaching Hospital with a history of a chronic ailment that had increased over the previous few months. A diagnosis of chronic renal failure and hepatitis was made and abdominal ultrasonography revealed the presence of gallbladder mucocele (GBM). The etiology, diagnosis, necropsy, and histopathological lesions were discussed.


Screening For S32g Mutation Of Bmp15 Gene In 18 Goat Breeds, Tao Feng, Ming-Xing Chu, Gui-Ling Cao, Dong-Wei Huang, Ran Di, Qiu-Yue Liu, Zhang-Yuan Pan, Mei Jin, Ying-Jie Zhang Jan 2014

Screening For S32g Mutation Of Bmp15 Gene In 18 Goat Breeds, Tao Feng, Ming-Xing Chu, Gui-Ling Cao, Dong-Wei Huang, Ran Di, Qiu-Yue Liu, Zhang-Yuan Pan, Mei Jin, Ying-Jie Zhang

Turkish Journal of Veterinary & Animal Sciences

In our early work, one mutation (A898G, which caused Ser32Gly (S32G) change of mature peptide) was identified in the goat BMP15 gene and the allele G was associated with high litter size in Jining Grey goats (the goat breed with the highest fecundity in China). The aim of this research was to investigate the genetic structure of the prolific BMP15 gene in 18 goat breeds reared in China, including 12 native breeds, 3 introduced breeds, and 2 cultivated breeds. Genomic DNA of 1011 goats was screened for the S32G mutation. The results showed that this mutation existed in all 3 …


Impact Of The Depletion Of High-Abundance Proteins In Blood Plasma/Serum On The Proteome Of These Media In Growing Farm Animals, Adam Lepczynski, Alicja Dratwa-Chalupnik, Agnieszka Herosimczyk, Katarzyna Staszak, Arleta Majewska, Malgorzata Ozgo Jan 2014

Impact Of The Depletion Of High-Abundance Proteins In Blood Plasma/Serum On The Proteome Of These Media In Growing Farm Animals, Adam Lepczynski, Alicja Dratwa-Chalupnik, Agnieszka Herosimczyk, Katarzyna Staszak, Arleta Majewska, Malgorzata Ozgo

Turkish Journal of Veterinary & Animal Sciences

The aim of the study was to use hexapeptide combinatorial libraries to evaluate the influence of high-abundance protein depletion on low-abundance protein enrichment, protein concentration, and 2D gel quality in plasma and serum proteomes of growing piglets and calves. Depleted and nondepleted samples of plasma/serum were resolved using 2D electrophoresis (2DE). Bioinformatic analysis of the 2D gels showed an increased number of detected spots on each gel after depletion of excess high-abundance proteins in calf plasma (+26.46%) and in piglet serum (+49.40%). Relative expression of selected low-abundance protein spots in the blood plasma/serum samples was higher after high-abundance protein depletion. …


Effects Of Supplementation Of Inorganic Sulfur On Some Biochemical Parameters In Angora Goat’S Diet Containing High Nitrate Levels, Mehmet Özdemi̇r, Abdullah Eryavuz, Gülcan Avci, Yavuz Osman Bi̇rdane, İsmai̇l Küçükkurt Jan 2014

Effects Of Supplementation Of Inorganic Sulfur On Some Biochemical Parameters In Angora Goat’S Diet Containing High Nitrate Levels, Mehmet Özdemi̇r, Abdullah Eryavuz, Gülcan Avci, Yavuz Osman Bi̇rdane, İsmai̇l Küçükkurt

Turkish Journal of Veterinary & Animal Sciences

The purpose of this study was to determine the effect of supplementation of sulfur to a diet containing sodium nitrate at a level that would cause chronic nitrate poisoning on some rumen and biochemical parameters in Angora goats. A total of 18 male Angora goats were used. The animals in the control group were fed the basal diet, while the animals were fed experimental concentrate containing 1500 mg/kg sodium nitrate in the nitrate group (N) and experimental concentrate containing 1500 mg/kg sodium nitrate plus 1.8% sodium sulfate in the nitrate-sulfur group (NS) throughout the study, which lasted for 6 months. …


Clinical Presentation And Ultrasonographic Findings In Buffaloes With Congestive Heart Failure, Hussein Awad Hussein, Rudolf Staufenbiel Jan 2014

Clinical Presentation And Ultrasonographic Findings In Buffaloes With Congestive Heart Failure, Hussein Awad Hussein, Rudolf Staufenbiel

Turkish Journal of Veterinary & Animal Sciences

The objective of this retrospective study was to describe the clinical presentation and ultrasonographic findings in water buffaloes suffering from congestive heart failure. Fifty-three buffaloes with congestive heart failure, which was confirmed postmortem, were included. The final diagnoses were traumatic pericarditis (n = 38) and vegetative endocarditis (n = 15). Ten healthy buffaloes were enrolled in this study as controls. The main clinical presentations were those related to right-sided congestive heart failure including peripheral edema, venous distension and jugular pulsation, and a sharp drop in milk yield. Only 5 buffaloes exhibited signs of left-sided heart failure. Ultrasonographic examinations showed cardiac …


Recent Advances In The Immunology And Uterine Microbiology Of Healthy Cows And Cows That Develop Uterine Disease, Klibs N. Galvao, Jose Eduardo P. Santos Jan 2014

Recent Advances In The Immunology And Uterine Microbiology Of Healthy Cows And Cows That Develop Uterine Disease, Klibs N. Galvao, Jose Eduardo P. Santos

Turkish Journal of Veterinary & Animal Sciences

Uterine diseases are highly prevalent in dairy cows. Causes of uterine diseases are multifactorial. There is good evidence for the susceptibility of the host and for the role of pathogenic bacteria, and less evidence for the effect of the environment. Uterine and leukocyte immune response is impaired early postpartum in cows that develop uterine disease. The decrease in immune function is associated with a decrease in calcium postpartum and an increase in NEFA and BHBA. Both endometrial cells and granulosal cells possess toll-like receptors (TLR) that can recognize and mount an inflammatory response to pathogen-associated molecular patterns (PAMPs) such as …


Maternal Embryokines That Regulate Development Of The Bovine Preimplantation Embryo, Peter James Hansen, Anna Carolina Denicol, Kyle Bradley Dobbs Jan 2014

Maternal Embryokines That Regulate Development Of The Bovine Preimplantation Embryo, Peter James Hansen, Anna Carolina Denicol, Kyle Bradley Dobbs

Turkish Journal of Veterinary & Animal Sciences

Proper embryonic development depends upon regulatory signals from the reproductive tract called embryokines. This review uses the cow as a model to understand the developmental processes controlled by embryokines. The focus is on 3 embryokines that have been shown to increase competence of embryos to survive after transfer to recipients: CSF2, IGF1, and DKK1. Together, these molecules regulate key events required for development of the embryo to the blastocyst stage including proliferation (enhanced by IGF1), development of the inner cell mass (regulated by CSF2), control of differentiation (modified by DKK1), and inhibition of apoptosis and stress-mediated developmental arrest (IGF1 and …


The Micrornas Important For Ovarian And Early Embryonic Development In Cattle, Lei Wang, Jianbo Yao Jan 2014

The Micrornas Important For Ovarian And Early Embryonic Development In Cattle, Lei Wang, Jianbo Yao

Turkish Journal of Veterinary & Animal Sciences

Recent progress in genomic analysis and other biochemical methods has led to the discovery of a large population of microRNAs (miRNAs), which have been demonstrated to play important roles in diseases and a wide range of developmental processes. Characterization of miRNA expression profiles in different stages of ovarian follicular development and early embryogenesis has suggested the potential roles of miRNAs in follicular development, maturation of oocytes, and preimplantation embryonic development. This review focuses on the current studies of miRNAs involved in ovarian and early embryonic development in cattle.


Polledness Intersex Syndrome In Goats – Molecular And Histological Aspects, Iwona Szatkowska, Daniel Zaborski, Witold Stanislaw Proskura, Sara Tabor Jan 2014

Polledness Intersex Syndrome In Goats – Molecular And Histological Aspects, Iwona Szatkowska, Daniel Zaborski, Witold Stanislaw Proskura, Sara Tabor

Turkish Journal of Veterinary & Animal Sciences

Polledness intersex syndrome (PIS) is a phenomenon associated with 2 distinct conditions occurring in goats, namely polledness and intersexuality. The first is a trait exhibiting complete dominance, whereas the latter is recessive. The extreme case of intersexualism is complete sex reversal, which is observed only in females (60,XX). The trait PIS is inherited in a recessive manner and caused by deletion of 11.7 kbp DNA on chromosome 1q43. Female goats with PIS exhibit a broad range of developmental abnormalities of the sexual system, from only subtle changes to complete sex reversal. The phenotypic diversity in goats with PIS is most …


New Understanding Of An Old Phenomenon: Uncontrolled Factors And Misconceptions That Cast A Shadow Over Studies Of The ‘Male Effect’ On Reproduction In Small Ruminants, Trina Jorre De St Jorre, Penny Alison Rhian Hawken, Graeme Bruce Martin Jan 2014

New Understanding Of An Old Phenomenon: Uncontrolled Factors And Misconceptions That Cast A Shadow Over Studies Of The ‘Male Effect’ On Reproduction In Small Ruminants, Trina Jorre De St Jorre, Penny Alison Rhian Hawken, Graeme Bruce Martin

Turkish Journal of Veterinary & Animal Sciences

In female sheep and goats, exposure to males can be used to induce ovulation and thus control and synchronise fertility. This 'male effect' offers simple, cost-effective, and hormone-free management of reproduction in flocks and herds but, despite decades of research, significant gaps in our knowledge impede commercial application. Many of these gaps can be traced back to misconceptions in the early documentation of the male effect, and others are the result of uncontrolled factors in experimental design. Consequently, it is often difficult to draw conclusions from past research, to further our understanding of the phenomenon, and to make recommendations for …


Cholesterol Efflux From Sperm: Approaches And Applications, Zahid Naseer, Ejaz Ahmad, Meli̇h Aksoy Jan 2014

Cholesterol Efflux From Sperm: Approaches And Applications, Zahid Naseer, Ejaz Ahmad, Meli̇h Aksoy

Turkish Journal of Veterinary & Animal Sciences

Following ejaculation, sperm appear morphologically mature, but functionally they are inactive. To attain functional capability or capacitation in vivo, sperm must reside in the female reproductive tract for a certain period of time before fertilization. In the female reproductive tract, cholesterol efflux from the sperm plasma membrane is considered vital for the initiation of capacitation. A number of in vitro studies have used biological and nonphysiological means to understand the phenomenon of cholesterol efflux and capacitation. In this review, we present a short overview of the role of cholesterol efflux in regulating sperm capacitation using various agents. This review provides …


The Avian Ovary And Follicle Development: Some Comparative And Practical Insights, Alan L. Johnson Jan 2014

The Avian Ovary And Follicle Development: Some Comparative And Practical Insights, Alan L. Johnson

Turkish Journal of Veterinary & Animal Sciences

This review focuses on recent research related to the avian ovary and, in particular, cellular mechanisms mediating embryonic growth of the ovary, postembryonic follicular development, and the process of follicular selection. Attention is drawn to a variety of fundamental, yet unresolved, aspects of ovarian function, several of which are uniquely related to avian reproductive strategies. Some notable differences in ovarian function between birds and the most closely related extant group, the crocodilians, are illustrated. Moreover, because the sauropsid (reptilian and avian) and synapsid (mammalian) lineages diverged shortly after the emergence of amniotes some 340 million years ago, consideration is given …


Sequencing-Based Polymorphism In Leptin Receptor Gene And Lack Of Association With Postpartum Anestrus In Murrah Buffaloes, Rajesh Kumar, Meenakshi Gupta, Syed Shafiq, Ashok Kumar Balhara, Sadeesh Em, Mayukh Ghosh, Inderjeet Singh Jan 2014

Sequencing-Based Polymorphism In Leptin Receptor Gene And Lack Of Association With Postpartum Anestrus In Murrah Buffaloes, Rajesh Kumar, Meenakshi Gupta, Syed Shafiq, Ashok Kumar Balhara, Sadeesh Em, Mayukh Ghosh, Inderjeet Singh

Turkish Journal of Veterinary & Animal Sciences

In order to detect polymorphism in exon 20 of the leptin receptor (LEPR) gene and its possible association with postpartum anestrus (PPA), peripheral blood samples were obtained from 40 Murrah buffaloes including 20 PPA (>120 days after parturition) and 20 normal cyclic (


Endocrine And Metabolic Mechanisms Of Embryo And Fetal Intrauterine Growth Retardation In Dairy Cows, Anatoliy Nezhdanov, Sergey Shabunin, Vitaliy Mikhalev, Nikolay Klimov, Anton Chernitskiy Jan 2014

Endocrine And Metabolic Mechanisms Of Embryo And Fetal Intrauterine Growth Retardation In Dairy Cows, Anatoliy Nezhdanov, Sergey Shabunin, Vitaliy Mikhalev, Nikolay Klimov, Anton Chernitskiy

Turkish Journal of Veterinary & Animal Sciences

This research was conducted to study hormonal and metabolic statuses and to determine ultrasound criteria for early diagnosis of fetal growth retardation syndrome (FGRS) in dairy cows of Black-motley (n = 41) and Red-motley (n = 56) Holstein breeds. Concentrations of progesterone, testosterone, 17beta-estradiol, dehydroepiandrosterone sulfate, cortisol, and triiodothyronine in blood serum were assessed by immune-enzyme analysis. Concentrations of plasma L-ascorbic acid and nitrogen oxides, serum immune globulin, middle molecular peptides, and bactericidal and lysozyme activity were determined by spectrophotometry. On days 38-40 and 60-65 of gestation, FGRS in cows was associated with hypoprogesteronemia, decrease of adrenal and thyroid gland …


Effect Of Inbreeding On Yield And Quality Of Embryos Recovered From Superovulated Holstein Cows, Jiri Bezdicek, Ludek Stadnik, Alexander Makarevich, Elena Kubovicova, Frantisek Louda, Zdenka Hegedusova, Radek Holasek, Jan Beran, Michaela Nejdlova Jan 2014

Effect Of Inbreeding On Yield And Quality Of Embryos Recovered From Superovulated Holstein Cows, Jiri Bezdicek, Ludek Stadnik, Alexander Makarevich, Elena Kubovicova, Frantisek Louda, Zdenka Hegedusova, Radek Holasek, Jan Beran, Michaela Nejdlova

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to evaluate the effect of inbreeding on the yield and quality of bovine embryos. The data were obtained from 125 Holstein cows superovulated on 3 farms in the Czech Republic. In total, 125 flushings of embryos were analyzed, from which 761 embryos were obtained. Of all donors, 36 cows were inbred (199 embryos) and 89 cows were noninbred (562 embryos). The inbred cows were divided into 2 groups according to their inbreeding coefficient (FX) level. The low FX group (FX 1.26%-1.56%) had 23 cows and 147 embryos, and the high FX group (FX 3.1%-25%) …


The Effect Of Short-Term Lupin (Lupinus Angustifolius) Feed Supplementation On Serum Steroid Hormones, Insulin-Like Growth Factor I, And Ovarian Follicular Development And Atresia In Merino Ewes, Radoslava Vlckova, Jan Posivak, Igor Valocky, Zuzana Kravcova, Andrea Eibenová, Drahomia Sopkova Jan 2014

The Effect Of Short-Term Lupin (Lupinus Angustifolius) Feed Supplementation On Serum Steroid Hormones, Insulin-Like Growth Factor I, And Ovarian Follicular Development And Atresia In Merino Ewes, Radoslava Vlckova, Jan Posivak, Igor Valocky, Zuzana Kravcova, Andrea Eibenová, Drahomia Sopkova

Turkish Journal of Veterinary & Animal Sciences

The effects of short-term lupin supplementation on ovulation rate in ewes during oestrous season are well known. In this study, we tested the effects of an 8-day lupin supplementation on folliculogenesis during anoestrus applied over the hormonal treatment of ewes. Fourteen anoestrous Merino ewes were assigned into 2 groups (n = 7 for each) in which the control group was fed a basal diet and the experimental group was supplemented with lupin groats (500 g per head per day). Both groups were treated intramuscularly with 12.5 µg/head lecirelinum (luteinising hormone releasing hormone super analogue) on the first day of supplementation …


Study Of Insulin-Like Growth Factor 1 Effects On Bovine Type A Spermatogonia Proliferation And Viability, Babak Qasemi Panahi, Parviz Tajik, Mansoureh Movahedin, Gholamali Moghaddam, Mohammad Hossein Geranmayeh Jan 2014

Study Of Insulin-Like Growth Factor 1 Effects On Bovine Type A Spermatogonia Proliferation And Viability, Babak Qasemi Panahi, Parviz Tajik, Mansoureh Movahedin, Gholamali Moghaddam, Mohammad Hossein Geranmayeh

Turkish Journal of Veterinary & Animal Sciences

Spermatogonial stem cells (SSCs) are the best candidates for exploitation in genetic improvement of cattle herds. In fact, SSCs transmit genetic information to future generations. In vitro improvement in proliferation and frozen-thawed SSCs numbers is therefore needed. In the present study, bovine SSCs were cocultured with Sertoli cells, with or without insulin-like growth factor 1 (IGF-1). During 16 days of incubation, 100 ng/mL IGF-1 showed higher efficacy in increasing the numbers and diameters of SSCs colonies than 40 and 150 ng/mL concentrations. Furthermore, 100 ng/mL IGF-1 increased the frozen/thawed cells’ viability rate. Overall, these data demonstrate that usage of 100 …


Investigation Of The Efficacy Of Tarantula Cubensis Extract (Theranekron D6) In The Treatment Of Subclinical And Clinical Mastitis In Dairy Cows, Kutlay Gürbulak, Aytaç Akçay, Kadi̇r Semi̇h Gümüşsoy, Birgit Sist, Simone Steiner, Murat Abay, Esra Canooğlu, Tayfur Bekyürek Jan 2014

Investigation Of The Efficacy Of Tarantula Cubensis Extract (Theranekron D6) In The Treatment Of Subclinical And Clinical Mastitis In Dairy Cows, Kutlay Gürbulak, Aytaç Akçay, Kadi̇r Semi̇h Gümüşsoy, Birgit Sist, Simone Steiner, Murat Abay, Esra Canooğlu, Tayfur Bekyürek

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to investigate the effects of the use of Theranekron alone or in combination with intramammary antibiotic therapy for the treatment of subclinical and clinical mastitis on the recovery rate from mastitis. The study material was composed of 177 mammary lobes of 63 Holstein cows with subclinical (groups S1 and S2) and clinical mastitis (groups C1, C2, C3, and C4). In all of the treatment groups, excluding group S2, the California mastitis test scores were determined to have decreased after treatment (P < 0.05). In groups C1, C2, C3, and C4, which were treated for clinical mastitis, the somatic cell count (SCC) values were observed to have decreased. The decrease in SCC values was statistically significant except in group C2 (P < 0.01). While the SCC values were observed to have decreased in group S1 (P > 0.05), which was treated with Theranekron alone, the same values were found to …