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Full-Text Articles in Life Sciences
Quantitative Electron Microprobe Analysis Of Cryosections, Albert J. Saubermann
Quantitative Electron Microprobe Analysis Of Cryosections, Albert J. Saubermann
Scanning Microscopy
There are numerous approaches to preparation and analysis of biological specimens which have been developed in many laboratories involving the use of cryosections. Because there is no clear consensus on how such samples should be produce or analyzed, it can be a formidable problem to choose or even review possible options. Therefore, it is the purpose of this tutorial to consider, in an instructional manner the problems of specimen preparation and analysis in terms of the solutions and procedures used by the author. The discussion, consequently. focuses on the problems of specimen preparation and analysis as seen by the author. …
A Comparison Of The Elemental Concentrations In Tissue Thymocytes From Diabetic And Non-Diabetic Rats, A. Warley
Scanning Microscopy
The elemental concentrations in thymocytes from control and diabetic rats were studied by use of the techniques of cryofixation and X-ray microanalysis to determine whether any changes occur in the diseased state. Decreases in the concentrations of the elements P and K were found in thymocytes from the subcapsular and cortical regions of the gland in thymus tissue taken 4 days after the onset of diabetes. A decrease in the concentration of Mg was also found in thymocytes from the subcapsular region of the gland. These changes suggest that the metabolism of thymocytes in vivo is altered in the diabetic …
Concentrations Of Elements In Dying Thymocytes From The Thymus Gland Of Diabetic Rats, Alice Warley, I. W. Morris
Concentrations Of Elements In Dying Thymocytes From The Thymus Gland Of Diabetic Rats, Alice Warley, I. W. Morris
Scanning Microscopy
Atrophy of the thymus gland occurs in rats made diabetic by a single injection of the drug streptozotocin. Histological studies show the presence of thymocytes with pyknotic nuclei in thymus tissue taken from diabetic animals. Analysis of the elemental content of the pyknotic cells was carried out on freeze dried frozen sections of thymus tissue using the technique of X-ray microanalysis. There was no loss of elements from cells which had undergone the early morphological changes characteristic of pyknosis and which showed the condensed chromatin of pyknotic nuclei. However as the cells shrank and lost the clear distinction between nucleus …