Life Sciences Commons^™

Measurement Of Fungicide Smoke Application By Electron Beam Analysis, C. R. Krause, C. C. Powell

Scanning Microscopy

Low volume fungicide smoke particle deposits were studied with scanning electron microscopy and energy dispersive X-ray analysis. Under greenhouse conditions, particles deposited on Rhododendron leaf surfaces and onto inert, carbon planchets adjacent to Rhododendron leaves were characterized by electron beam analysis. Angular particles containing chlorine, identified as vinclozolin deposition, were quantified on a per unit area basis. Leaf samples collected from plants immediately adjacent to the smoke applicator had more particles than leaf samples collected elsewhere. More deposition was measured on leaf surfaces than on inert planchet surfaces. This study increased our knowledge about the effectiveness of using less fungicide …

The Use Of Scanning Electron Microscopy For Investigations Into The Three Dimensional Organisation Of The Interphase Nucleus, T. D. Allen, P. M. O'Connor

Scanning Microscopy

In an attempt to elucidate three dimensional information on the organisation of the nucleus, nuclei have been extracted from ascites tumour cells or tissue culture cells by a variety of biochemical techniques, and prepared for high resolution scanning electron microscopy using an osmium-thiocarbo-hydrazide infiltration procedure which has previously proved successful for analysis of chromosome structure. Nuclei were prefixed with either Methanol-Acetic acid, glutaraldehyde or formaldehyde and then extracted by a variety of detergents with the aim of a 'biochemical dissection' of their outer elements to allow surface visualisation of the nuclear lamina. Continued extraction removed all aspects of the nuclear …

Fine Structure And Organ Culture Of Chick Embryo Dorsal Aorta, C. Ward Kischer, Michael Jaqua

Scanning Microscopy

Embryonic blood vessels have not been grown in organ culture, in a way which might easily submit them to studies of vascular organogenesis. The chick embryo dorsal aorta is easily accessible and relatively simple to explant to culture. Its organ culture may provide a model for wounding and repair of the intima and/or media and provide a model for studies of growth (or maintenance) and differentiation. Fresh dorsal aorta and its organ culture from the chick embryo was characterized morphologically by light microscopy scanning (SEM) and transmission electron microscopy (TEM) from days 4 to 18. Explants were incubated up to …

Observations Of Colloidal Gold Labelled Platelet Microtubules: High Voltage Electron Microscopy And Low Voltage-High Resolution Scanning Electron Microscopy, R. M. Albrecht, J. Prudent, S. R. Simmons, J. Pawley, J. J. Choate

Scanning Microscopy

18 nm colloidal gold-antitubulin and 4 nm colloidal gold-antitubulin were used to label microtubules in adherent, fully spread platelets. Both sizes of marker effectively labelled microtubules in the partially extracted platelets. However only the 4 nm gold penetrated the dense microfilament matrix of the inner filamentous zone so that portions of microtubules within this cytoskeletal zone could be tracked. The gold marker could be visualized well with 1 MeV high voltage transmission EM and with 5 kV or greater secondary imaging or 20 kV backscattered imaging of carbon only coated samples. 1 kV secondary imaging permitted high resolution imaging of …

Number 18 (December 1988), Southern Fishes Council

Southeastern Fishes Council Proceedings

(December 1988) - Pit-Ridge Nest Construction and Spawning Behavior of Semotilus lumbee and Semotilus thoreauianus. By W.S. Wollcott and E.G. Maurakis, 2 pp.

A Need for Clarification of the Concept of Nest Building Among Cyprinid Minnows. By W.S. Wollcott and E.G. Maurakis, 1 p. plus Curatorial Notes and News Notes.

Curatorial Notes, Herbert Boschung

Southeastern Fishes Council Proceedings

No abstract provided.

A Need For Clarification Of The Concept Of Nest Building Among Cyprinid Minnows, William S. Woolcott, Eugene G. Murakis

Southeastern Fishes Council Proceedings

No abstract provided.

Pit-Ridge Nest Construction And Spawning Behaviors Of Semotilus Lumbee And Semotilus Thoreauianus, William S. Woolcott, Eugene G. Murakis

Southeastern Fishes Council Proceedings

No abstract provided.

Business Meeting Minutes, Southeastern Fishes Council

Southeastern Fishes Council Proceedings

No abstract provided.

Regional Reports, Southeastern Fishes Council

Southeastern Fishes Council Proceedings

No abstract provided.

News Notes, Southeastern Fishes Council

Southeastern Fishes Council Proceedings

No abstract provided.

Isolated Cataplexy: A Familial Study, Kristyna M. Hartse, Frank J. Zorick, Jeanne M. Sicklesteel, Thomas Roth

Henry Ford Hospital Medical Journal

Four members of a family, a mother, her two sons, and the mother's second cousin, presented with a history of isolated cataplexy without excessive daytime sleepiness or other symptoms typical of narcolepsy. They were polygraphically monitored during one night followed by a day of multiple nap tests. No subject manifested objective daytime sleepiness as measured by short latencies to sleep onset during daytime naps, and no subject exhibited periods of sleep onset rapid eye movement sleep in either the night or nap recordings. The incidence of isolated cataplexy in this family was traced by history through six generations. We conclude …

Front Matter

Henry Ford Hospital Medical Journal

No abstract provided.

The Expanding Spectrum Of Pediatric Practice, Jeffrey M. Devries

Henry Ford Hospital Medical Journal

No abstract provided.

Duplication And Deletion 11q23-Q24 Recombinants In Two Offspring Of An Intrachromosomal Insertion ("Shift") Carrier, Miriam G. Forsythe, Hugh Walker, Lester Weiss, Jacquelyn R. Roberson, Maria J. Worsham, V. Ramesh Babu, Daniel L. Van Dyke

Henry Ford Hospital Medical Journal

Few examples of intrachromosomal insertions have been described. These usually result from deletion of a segment of chromosome material, with insertion of the deleted material elsewhere on the same chromosome. Previous insertional translocations have been identified through a proband who has either a deletion or a duplication of the inserted segment. We describe a family which has two probands, one with a duplication and one with a deletion of the inserted segment. The unbalanced chromosomes probably resulted from an uneven number of crossovers between the breakpoints in the chromosome 11 of the father, who carries a balanced intrachromosomal insertion of …

Perinatal Idiopathic Hemochromatosis, Usha B. Raju, Sudhakar Ezhuthachan, Chan K. Ma

Henry Ford Hospital Medical Journal

We report the clinicopathologic features of an infant who died of a rare form of perinatal cirrhosis associated with idiopathic hepatic and extrahepatic parenchymal siderosis. The infant appeared normal at birth but soon became severely ill, following a progressively downhill course associated with hypoglycemia, metabolic acidosis, bleeding diathesis, jaundice, and shock. The infant died at 7 days of age. The manifestations were those of hepatic failure but mimicked sepsis and disseminated intravascular coagulation. Cirrhosis, giant cell transformation, and parenchymal iron deposition characteristic of perinatal idiopathic hemochromatosis, a recently emerging clinicopathologic entity of unknown etiology, were present in this infant. These …

Bladder Catheter Knotting: A Case Report, Mark G. Goetting

Henry Ford Hospital Medical Journal

No abstract provided.

The Development Of Pediatrics In The United States, Jay H. Mayefsky

Henry Ford Hospital Medical Journal

No abstract provided.

Serum Creatinine Concentrations In Healthy Newborns: Reference Ranges During The First Five Days Of Life, Jennie Andreson, Jaime Alfredo Zegarra, Carlos Alberto Estrada, Francis Dumler

Henry Ford Hospital Medical Journal

Serum creatinine concentrations were studied in 52 healthy, full-term infants (29 males and 23 females) during the first five days of life. At birth, mean serum creatinine concentration was 0.76 ± 0.13 mg/dL. At 6 hours of life, values increased to 0.97 ± 0.11 mg/dL (P < 0.001) and remained elevated for 24 hours. Values then returned to baseline so that serum creatinine concentrations were 0.81 ± 0.15 mg/dL at 48 hours and 0.6l ± 0.15 mg/dL at 3 to 5 days of age. At birth, serum creatinine concentrations were higher in males than in females (0.80 ± 0.13 versus 0.71 ± 0.11 mg/dL, respectively; P < 0.009). However, the increases in serum creatinine concentration observed after birth were similar in both sexes. This is the first report of a prospective longitudinal study of serum creatinine concentrations between birth and 3 to 5 days of age in a Hispanic population. In addition, data were analyzed by sex. The results are a useful reference for normal serum creatinine concentrations in early life.

Ovarian Failure In A Chromosomally Competent Adolescent Female: A Case Report, T. Jann Caison-Sorey

Henry Ford Hospital Medical Journal

Primary ovarian failure in adolescence is uncommon. This report depicts such a case in a 17 1/2-year old girl whose investigation revealed elevations in the follicle-stimulating hormone and luteinizing hormone levels in association with a low estrogen level. The blood leukocyte chromosome analysis with Giemsa banding revealed a 46,XX chromosomal complement. Pelvic ultrasound disclosed a rudimentary uterus and a small left ovary. Hormonal replacement has resulted in advancement of secondary sexual characteristics and monthly withdrawal bleeding.

Validation Of Electronic Rectal Thermometry, Mark G. Goetting, Karen Stratton

Henry Ford Hospital Medical Journal

Electronic rectal thermometry is performed routinely in most medical centers. While electronic thermometry has been validated at the oral site, rectal measurement has not. We performed a controlled study on 84 children comparing mercury-in-glass and electronic thermometry at the rectal site. No significant difference occurred in temperatures between the two methods. We conclude that the electronic thermometer is a valid instrument to measure rectal temperature.

A Practical Approach To Infantile Apnea, Gregory Preston

Henry Ford Hospital Medical Journal

No abstract provided.

Pediatric Emergency Care: A Survey Of Michigan Emergency Departments, David J. Treloar, Gregory Preston, Fred Lamb

Henry Ford Hospital Medical Journal

Recent studies suggest a lack of preparation in emergency medical systems for pediatric patients. Michigan emergency departments were surveyed to determine their degree of preparedness regarding equipment, space, and pediatric on-call coverage. More than 50% of responding emergency departments did not have a pediatrician on call and lacked a pediatric "crash cart," and 34% had to send their personnel to other areas in the hospital to obtain pediatric equipment during resuscitations. This survey suggests a lack of preparation for pediatric emergencies in Michigan.

The Circumcision Decision: A Plea For Informed Consent, Theresa Spinelli

Henry Ford Hospital Medical Journal

No abstract provided.

The Pediatrician's Guide To Food Allergy, John A. Anderson

Henry Ford Hospital Medical Journal

Food allergy occurs in as many as 5% of infants less than 1 year old. Most food sensitivities disappear by age 3 to 4. Most immunologically mediated food allergy reactions are due to type I IgE mechanisms, with skin, gastrointestinal, and respiratory signs and symptoms the most common manifestations. U sing the double-blind, placebo-controlled food challenge (DBPCFC) technique and atopic dermatitis as a model, the most common foods to which children are allergic are cow's milk, eggs, peanuts, wheat, fish, and soy proteins. Anaphylactic sensitivity in older children often involves crustacean seafoods (eg, shrimp), nuts, peanuts, fish, and eggs. Such …

Attention Deficit Disorder: An Overview, Wiley C. Rasbury

Henry Ford Hospital Medical Journal

No abstract provided.

Prevalence Of Respiratory Symptoms And Allergic Sensitivity In Multiply Handicapped Children, Dennis R. Ownby, Mary Logan, Ruth Ann Belfi

Henry Ford Hospital Medical Journal

Among the many challenges of providing care for children with multiple physical and developmental handicaps is the proper management of their respiratory difficulties. This study was designed to evaluate the prevalence of respiratory symptoms in children attending a multidisciplinary clinic for handicapped children. We also used several methods to estimate the prevalence of allergic sensitivity in children with respiratory complaints. Questionnaires concerning respiratory symptoms were completed by the parents of 104 patients. The most frequent complaint reported was snoring in 41% of the children. Of the 39% who had multiple respiratory symptoms, 85% had detectable allergen- specific IgE to cat, …

Child Life: Meeting The Psychosocial Needs Of Children And Families In The Hospital, Christine O. Newman, Carol G. Klingbeil, Roberta B. Abrams

Henry Ford Hospital Medical Journal

No abstract provided.

Adolescent Pregnancy And Sexually Transmitted Diseases: Case Report, Ruby L. Holloway, Richard Smith

Henry Ford Hospital Medical Journal

More than 1 million teenagers become pregnant every year in the United States. Nearly one-half of the pregnancies are carried to term, while a similar number end in abortion. In Michigan this amounts to approximately 34,000 teenage pregnancies (ages 15 to 19). These adolescent patients have a high incidence of sexually transmitted diseases (STDs). Of the pregnant teens at Henry Ford Hospital. 60% present with one or more STDs. We discuss the case of a 13-year-old pregnant girl who presented at five weeks estimated gestation with gonorrhea, chlamydia, and appendiceal perforation, resulting in subsequent spontaneous abortion. Further discussion focuses on …

The Role And Success Of School-Based Clinics, Terence A. Joiner

Henry Ford Hospital Medical Journal

No abstract provided.