Life Sciences Commons^™

Neurobehavioral Symptoms In Mild Primary Hyperparathyroidism: Related To Hypercalcemia But Not Improved By Parathyroidectomy, Gregory G. Brown, Richard C. Preisman, Michael Kleerekoper

Henry Ford Hospital Medical Journal

The neurobehavioral symptomatology of severe primary hyperparathyroidism (PHPT) has been thoroughly described. Less is known about more mild cases. We evaluated 34 patients with mild PHPT and followed 19 for an average of six months. Of those cases followed, ten had parathyroidectomy (PTX). Patients with no neurobehavioral abnormalities had the lowest serum calcium levels (10.9 ± 0.88 mg/dL), patients with signs of affective disorder had intermediate levels (11.25 ± 0.66 mg/dL), and patients with signs of cerebral dysfunction had the highest levels (12.17 ± 1.17 mg/dL). Serum calcium significantly correlated with motor speed, psychomotor speed, fluid intelligence, and short term …

Management Of Extramedullary Plasmacytomas, James A. Bonner, Jadranka Dragovic, Michael P. Abrash

Henry Ford Hospital Medical Journal

From 1965 through 1982. 12 patients with solitary, biopsy-proven extramedullary plasmacytomas (EMPs) were treated at our institution with surgery alone or biopsy followed by radiation therapy. The median age of the patients was 67 years, and the median-follow-up was 58 months. Of the seven patients who were treated by primary surgery, three developed recurrent or distant disease. Five patients underwent biopsy followed by radiation therapy, and none of their tumors progressed. Our series and a review of the literature show that occasionally EMPs will incompletely regress even after high doses of radiation (greater than 65 Gy); however, we recommend that …

An Overview Of Magnetic Resonance Imaging Of The Appendicular Musculoskeletal System, Sharon C. Siegel, Allan M. Haggar, Jerry W. Froelich, Burton I. Ellis

Henry Ford Hospital Medical Journal

No abstract provided.

Obstructing Giant Pseudopolyposis Of The Colon In Ulcerative Colitis, Roberta E. Sonnino, Farouck N. Obeid

Henry Ford Hospital Medical Journal

Pseudopolyposis of the colon is a well-recognized local complication of ulcerative colitis. Giant pseudopolyposis is uncommon, and its presentation as a complete obstruction of the colon is a rare occurrence. We report such a case, where retrograde flow of barium on double-contrast barium enema was completely obstructed at midtransverse colon. After resection by total abdominal colectomy, pathology examination ofthe specimen confirmed massive pseudopolyposis without malignancy. A brief review of the pathology and current literature is presented.

Melvin A. Block, Md, Gary B. Talpos

Henry Ford Hospital Medical Journal

No abstract provided.

Ocular Irrigating Solutions: A Comparison Between Balanced Salt Solution And L-410 (Po-Eis), Philip C. Hessburg, Paul M. Johnston

Henry Ford Hospital Medical Journal

The ability of two ocular irrigating solutions to reduce postoperative corneal edema after intracapsular cataract extraction was compared using ultrasonic pachymetry. All patients had anterior chamber intraocular lenses implanted. The solutions were balanced salt solution and solution L-410 (PO-EIS), an eye irrigation solution containing dextran 40 and bicarbonate. Corneas irrigated with either solution showed no significant difference in postoperative edema. The use of L-410 (as an alternative to balanced salt solution) may not be necessary in intracapsular cataract extraction.

Prolonged Remission Of Cushing's Disease Following Bromocriptine Therapy, Malachi J. Mckenna, Marc Linares, Raymond C. Mellinger

Henry Ford Hospital Medical Journal

A 33-year-old woman developed hypercorticism of fulminant onset following delivery of a full-term, normal child. An ectopic hormone-producing neoplasm was excluded by extensive studies. Pituitary dependent hypercorticism of intermediate lobe origin was suggested on the basis of onset following pregnancy, failure of Cortisol suppression by high-dose dexamethasone, hyperresponsiveness of prolactin to thyrotropin-releasing hormone stimulation, and reduction in adrenocorticotropin titers following oral administration of bromocriptine. Initial remission of disease achieved with bromocriptine was followed by recurrence on discontinuation of the agent. However, complete remission which occurred following a prolonged course of bromocriptine has persisted for a total of 22 months.

Hyperthyroidism Due To A Thyrotropin-Secreting Pituitary Microadenoma, Jeffrey A. Jackson, Mitchell Smigiel, John F. Green Jr.

Henry Ford Hospital Medical Journal

A 52-year-old man presented with elevated thyroid hormone levels and an inappropriately normal serum thyrotropin (TSH) (4.0 μU/mL: normal 0.5 to 6.0 μU/mL). Computed tomography suggested an intrasellar mass without radiographic sellar enlargement. Serum alpha-subunit was elevated with flat responses of both alpha-subunit and TSH to thyrotropin-releasing hormone. Transsphenoidal adenomectomy resulted in clinical and biochemical cure with subsequent development of hypothyroidism with otherwise preserved anterior pituitary function. Pathologic studies demonstrated an 8 mm typical thyrotroph cell adenoma. Early diagnosis of such tumors requires a high index of clinical suspicion and may be facilitated in the future by utilization of highly …

Primary Aldosteronism At Henry Ford Hospital In The 1980s, Max Wisgerhof

Henry Ford Hospital Medical Journal

This report describes the experience with the diagnosis and treatment of primary aldosteronism at Henry Ford Hospital since 1980. Of the 28 patients who received the diagnosis. 13 had unilateral primary aldosteronism and 15 had idiopathic hyperaldosteronism. Individual cases are used to demonstrate clinical points. The clue to the presence of primary aldosteronism in a hypertensive patient is hypokalemia. The diagnosis is established by showing 1) high plasma aldosterone after intravenous saline or high urinary aldosterone after treatment with sodium chloride orally, and 2) low stimulated plasma renin activity. Treatment with potassium supplement should be given during the testing. Unilateral …

Unusual Lymphomas Developing In Chronic Lymphocytic Leukemia, Sundara B. K. Raman, Sheikh M. Saeed, Joseph P. Abraham

Henry Ford Hospital Medical Journal

We report three patients with chronic lymphocytic leukemia (CLL) who developed malignant lymphomas of unusual character and modes of presentation. Two of the patients had received low doses of chlorambucil for several years before they developed malignant lymphoma, diffuse, large cell type (LCL). In one of these patients LCL manifested as a grossly evident osteolytic lesion. In the second patient LCL developed initially as a localized lesion in the iliac bone. Both patients died within a few weeks after LCL was diagnosed. The third patient, who was found to have CLL during a routine examination, did not receive any therapy …

T-Cell Lymphoma In Renal Transplant Recipient, Koichi Maeda, Leslie Bricker, Chan K. Ma, Michael J. Deegan

Henry Ford Hospital Medical Journal

A 35-year-old woman, who had a renal transplant flve years ago, developed malignant lymphoma of the mediastinum. The lymphoma was of lymphoblastic type and had T-cell immunophenotype. Most transplant-related lymphomas are of B-cell type. T-cell lymphoma in this setting is extremely rare, and the mechanism of development may be different from that of B-cell lymphomas.

Book Review: Guides To Clinical Aspiration Biopsy: Thyroid, John E. Brunner

Henry Ford Hospital Medical Journal

No abstract provided.

Back Matter

Henry Ford Hospital Medical Journal

No abstract provided.

Quantitative Morphology Of Synaptic Plasticity In The Aging Brain, Carlo Bertoni-Freddari, William Meier-Ruge, Jurg Ulrich

Scanning Microscopy

Quantitation of synaptic ultrastructural changes is of great importance in neurobiology, since merely qualitative alterations, if not extreme, are not readily detectable. In the present paper we discuss our previous and present findings on the number (numerical density: Nv), size (average length of the synaptic profiles: L) and surface contact area (surface density: Sv) of the synaptic junctions in aging rodent and human brains. We found that number and size of the synapses are in a close inverse relationship so as to maintain the total surface contact area among the nerve cells constant. These three parameters are closely related to …

Transit Time Effect On Voltage Contrast In The Stroboscopic Scanning Electron Microscope, K. Nakamae, H. Fujioka, K. Ura

Scanning Microscopy

Voltage contrast varies if the specimen voltage changes within the secondary electron transit time through the specimen electric field. This effect would affect the time resolution in stroboscopic scanning electron microscopy. The method to calculate the transit time effect that was described in a previous paper is reviewed. The calculated results agreed well with the experiment in which a specially designed specimen is used to estimate the applied voltage as exactly as possible.

Microcorrosion Casting In Normal And Pathological Biliary Tree Morphology, E. Gaudio, L. Pannarale, F. Carpino, G. Marinozzi

Scanning Microscopy

The organization of the intrahepatic biliary tree was studied in three dimensions by scanning electron microscopic (SEM) corrosion casts, in normal and cholestatic rat liver.

In the normal liver the observation revealed the features of the biliary passages from the bile canaliculi to the canaliculo-ductular junction, to the ductules and the bile ducts, confirming previous SEM observations.

In cholestatic liver, the modifications and the proliferation of bile ductules appear clearly.

Resin flow from canalicular to sinusoidal network was never observed.

The method was found to be very useful in the evaluation of the architecture of the intrahepatic biliary tree, under …

Extraneous Background-Correction Program For Matrix Bound Multiple Point X-Ray Microanalysis, W. C. De Bruijn, M. P. C. Van Miert

Scanning Microscopy

A program is described that allows online determination of extraneous background in multiple point X-ray microanalytical matrices. The program is based upon the calculations of the extraneous background for the film (when present), the standard and the unknown by (100 sec.) point analysis. The program searches for a peak-free part of the spectrum in which the calculated value for the extraneous background is about equal to the value in this region of the spectrum (=b_e). Online the contents of this b_e-region is subtracted from an unmanipulated continuum region in the vicinity of the element present in …

Effects Of Cytochalasin B And Colchicine On The Morphology Of Sw-13 Human Adrenal Cortical Tumor Cells In Culture, S. A. Murray, H. N. Tung

Scanning Microscopy

Human adrenal cortical tumor cells (SW-13) grow into a typical epithelial cell monolayer when seeded onto culture dishes. The cells of the SW-13 population monolayer appear flattened with few conspicuous surface features. The cells are attached to one another at their lateral borders and are arranged in a cobblestone-like manner. Following Triton X-100 extraction, the distribution of the cytoskeletal elements was observed with scanning electron microscopic techniques to correspond to the shape of the non-extracted cell. Changes in the distribution and morphology of projections on the cell surface as well as changes in cell shape were revealed after treatment of …

Contrasting Views On The Organization Of Cytoplasm, J. S. Clegg

Scanning Microscopy

The widely held conception of cytoplasm as a concentrated solution of dissolved proteins and other macromolecules, metabolites and inorganic ions, within which the various organelles and other formed elements are suspended appears to be a gross over-simplification. Evidence to be reviewed briefly here, from a wide variety of experiments and cell types, indicates that a much more extensive organization exists, in animal cell cytoplasm at least. Results from experiments on dextran sulfate permeabilized L-929 cells will be presented which support that organized paradigm. It seems likely that this intracellular architecture also exerts a strong influence on the water in the …

Presence Of Calcium Oxalate Crystals In The Mammalian Thyroid Gland, Raymond L. Hackett, Saeed R. Khan

Scanning Microscopy

Birefringent crystals of calcium oxalate have been previously identified in the colloid of human thyroid glands. We found such crystals in 19/20 adult thyroids at autopsy, in 4/ 20 infants at autopsy, and, using frozen sections, in 19/20 thyroids partially or totally removed at surgery. These crystals were soluble in hydrochloric acid, insoluble in acetic acid, and contained only calcium by energy dispersive X-ray microanalysis, confirming their calcium oxalate character. Similar crystals were found in equine and ovine thyroids.

Tip-Techniques For Microcharacterization Of Materials, Y. Martin, C. C. Williams, H. K. Wickramasinghe

Scanning Microscopy

The invention of the Scanning Tunneling Microscope has stimulated the development of new techniques for microcharacterization of materials, which are based on the use of a very fine tip. Two of these techniques have emerged about one year ago, the Thermal Profiler and the Atomic Force Microscope. Both techniques have recently demonstrated the capability to profile and image conductors and insulators. The resolution attained varies from approximately 50 nm by the Thermal Profiler to a few nanometers with the Atomic Force Microscope, therefore competing with the resolution obtained with electron beam microscopy. We shall describe the principle of these techniques, …

Arterial Elastin As Seen With Scanning Electron Microscopy: A Review, Margot R. Roach, S. H. Song

Scanning Microscopy

All large arteries contain elastin, collagen, and muscle which can be seen with light microscopy and transmission electron microscopy. Elastin forms an internal elastic lamina (IEL) in all arteries, but also forms multiple fenestrated sheets in the media of the aorta and other large arteries. The fenestrations in the media are larger than those in the IEL. The adventitial elastin is more fibrous and often contains tubular elastin surrounding vasa vasorum when prepared by removing all non-elastin by placing the aorta in 0.1 N NaOH at 70-75°C for five hours. The fenestrations are larger near branches and in an experimentally …

Interferon-Induced Surface Alterations In Hairy Cells. A Review, Haim Gamliel, Dorit Gurfel, Shi-Hua Wu, Harvey M. Golomb

Scanning Microscopy

Hairy cells (HCs), derived from the peripheral blood and spleen of hairy cell leukemia (HCL) patients, constantly displayed both ruffles and microvilli. HCs which were kept in culture for up to three days exhibited extremely polarized and active surfaces with elongated microvilli and exaggerated "spiked" ruffles. Cells derived from 11 cases of HCL were treated with alpha-interferon (IFN) in-vitro and examined by immuno -scanning electron microscopy (immuno-SEM). In 8 cases, up to one-third of the IFN-treated hairy cells displayed deformed surfaces with "bubbling" membrane and markedly villous bud-like formations. Monoclonal antibodies (MoAb), used in conjunction with immuno-qold labeling, facilitated better …

Enhanced Floral Analysis By Low Temperature Scanning Electron Microscopy, Brenda Brookes, Ernest Small

Scanning Microscopy

Traditional methods of preparation of botanical specimens for scanning electron microscopy (SEM) have proven to induce artifacts in some specimens which often reduce quality of resulting images, and are highly misleading for taxonomic purposes. The advantages of low temperature SEM are illustrated by an investigation of freshly collected flowers, using a cryo-system interfaced to an SEM. This method overcame the deficiencies of traditional procedures to produce exceptional images of floral surfaces in their natural state. Depending on the nature of the material under investigation, the method my be indispensable (e.g., for accurate details of petal surfaces), preferable (e.g., glandular hairs …

Myocyte Swelling And Plasmalemmal Integrity During Early Experimental Myocardial Ischemia In Vivo, Martin D. Sage, Robert B. Jennings

Scanning Microscopy

Using scanning and transmission electron microscopy, the structure of myocytes early in the phase of irreversible injury induced by 40 minutes of severe regional ischemia has been investigated, paying particular attention to the effects of cell swelling on the SEM appearance of the myocytes. Swollen myocytes showed an increased space beneath the plasmalemma and between organelles. True subsarcolemmal blebs were not seen and the attachment complexes between the Z-band and the underlying myofibrils remained intact. The proportion of the PS face of the plasmalemma which appeared "en face" (0.70%, SD:1.22 vs 5.0196, SD:3.72) in freeze-fracture faces of ischemic tissue was …

The Effect Of Fatigue On Essential Element Distribution In The Rabbit Masseter, M. B. Engel, J. K. Bressman

Scanning Microscopy

The rabbit masseter is composed of mainly fast fatiguable type IIB fibers which give a positive mATPase reaction at pH 9.3. To study the effect of fatigue on the distribution of some crucial elements, the masseter was stimulated electrically for 45 minutes. The contralateral muscle served as a control. Control and experimental tissues were rapidly excised, and frozen in isopentane cooled to -150°C in liquid nitrogen. Sections were cut in a cryostat and prepared by freeze-drying. Water content of fatigued muscle was significantly higher than controls: 79.3% versus 77.3%. The fatigued state was verified by glycogen depletion viewed in PAS-stained …

Materials Analysis By Mass Spectrometry Of Sputtered Neutrals, Hans Oechsner

Scanning Microscopy

Mass spectrometric detection of neutral surface particles released by ion bombardment has become an important method for surface and depth profile analysis. Its fundamental difference to secondary ion mass spectrometry SIMS is the separation of the formation of the analyzed particles and their ionization. Hence, matrix and selectivity effects influencing SIMS signals in a mostly unknown manner via the ionization process in secondary ion emission are avoided in general.

The different techniques being presently employed for the necessary postionization of the sputtered neutrals are reviewed and discussed with respect to their potentialities. Secondary Neutral Mass Spectrometry (SNMS) using efficient postionization …

3-Dimensional Imaging Of Biological Structures By High Resolution Confocal Scanning Laser Microscopy, G. J. Brakenhoff, H. T. M. Van Der Voort, E. A. Van Spronsen, N. Nanninga

Scanning Microscopy

Imaging in confocal microscopy is characterized by the ability to make a selective image of just one plane inside a specimen, virtually unaffected -within certain limits-by the out-of-focus regions above and below it. This property, called optical sectioning, is accompanied by improved imaging transverse to the optical axis. We have coupled a confocal microscope to a computer system, making the combination of both an excellent instrument for mapping the 3-dimensional structure of extended specimens into a computer memory/data array. We measured that the volume element contributing to each data point has, under typical fluorescence conditions, a size of 0.2 x …

Junction Electron-Beam-Induced Current Techniques For The Analysis Of Photovoltaic Devices, R. J. Matson

Scanning Microscopy

A number of useful electron-beam-induced current (EBIC) techniques have evolved through the study of junction behavior in photovoltaic (PV) devices in cross section as a function of light and voltage bias, temperature, and electron beam scanning parameters. The necessary hardware modifications, the techniques themselves, and their applications are presented. In the case of PV devices, light and/or voltage biasing the entire device while electron probing it in cross section permits the observation of the distribution of the narrowing or extension of the space-charge region. Monitoring the junction behavior as a function of temperature has at least two applications. In situ …

Ion And Water Retention By Permeabilized Cells, Carlton F. Hazlewood, Miklos Kellermayer

Scanning Microscopy

Nonionic detergents, Triton X-100 and Brij 58, removed, within 2-5 minutes, lipid membrane of suspended thymus lymphocytes and monolayer H-50 cells grown in culture. Studies of hydration, ionic asymmetry, and ionic and protein release kinetics were conducted on these membraneless cellular preparations. The hydration of nuclei isolated by Triton X-100 procedures appears to be influenced strongly by the monovalent ionic concentration of the buffer bathing the organelles. The putative monovalent ionic concentration of the cellular aqueous phase (i.e., 150 meq/L) caused nuclei to swell and coalesce. Monovalent ionic concentrations of 30 meq/L or less caused minimal changes in volume and …