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Full-Text Articles in Life Sciences

Soil Enzyme Activities As Bio Indicators Of Soil Ph And Fertility In Temperate Grassland, Iker Mijangos, A. Ibarra, Isabel Albizu, Sorkunde Mendarte, Carlos Garbisu Aug 2023

Soil Enzyme Activities As Bio Indicators Of Soil Ph And Fertility In Temperate Grassland, Iker Mijangos, A. Ibarra, Isabel Albizu, Sorkunde Mendarte, Carlos Garbisu

IGC Proceedings (1997-2023)

In recent years, biological indicators are being used to estimate the continued capacity of a given soil to function (i.e., soil health). After all, biological processes are intimately linked with the maintenance of soil structure and fertility, being more sensitive to changes in the soil than conventional physicochemical parameters. Soil enzymes, as mediators and catalysts of vital soil functions, offer great potential as integrative indicators of soil health (Dick et al., 1996). The main aim of the current work was to study the potential of soil enzyme activities as biological (more precisely, biochemical) indicators of soil physicochemical …


Effect Of Ferulic Acid Esterase Enzyme Application On The In Vitro Digestibility And In Situ Rumen Degradability Of Tropical Grasses, Adegbola T. Adesogan, N. Krueger, C. R. Staples, D. B. Dean, W. Krueger May 2023

Effect Of Ferulic Acid Esterase Enzyme Application On The In Vitro Digestibility And In Situ Rumen Degradability Of Tropical Grasses, Adegbola T. Adesogan, N. Krueger, C. R. Staples, D. B. Dean, W. Krueger

IGC Proceedings (1997-2023)

Tropical grasses are the primary staple diet of most of the domesticated ruminants in the tropical and subtropical regions of the world. However, dry matter (DM) digestibility (DMD) and intake levels of these C4 grasses are considerably low, partly because of high lignin contents. Ferulic acid also impedes fibre digestion in such forages due to formation of cross linkages with digestible xylans. Certain esterase enzymes have been shown to cleave ferulic acid cross linkages in wheat bran but no studies have examined whether such enzymes can be used to increase the digestion of tropical grasses. Therefore, the objective of …


Effects Of Different Additives On Silage Quality Of Napiergrass, Y. K. Cheng, C. S. Chen, P. W. Peng Dec 2021

Effects Of Different Additives On Silage Quality Of Napiergrass, Y. K. Cheng, C. S. Chen, P. W. Peng

IGC Proceedings (1997-2023)

Objectives of this experiment were to determine the effects of different additives on silage quality of napiergrass (Pennisetum purpureum). The cultivar of TLG2 with high water soluble carbohydrates was used to making silages added with or without 5 and 10% corn meal or wheat bran, and then treated with or without enzyme. Crude protein, acid detergent fiber, neutral detergent fiber, water soluble carbohydrates and volatile fatty acid contents of silage were determined to evaluate silage quality. From the results, it showed that var.TLG2 could be making good silages without any additives but the dry matter was low. Adding …


Effects Of Different Enzyme Combination And Dissociation‐Time On The Protoplast Isolation Of Alfalfa, Aiping Chen, Yupeng Luo, Run Yan, Bo Zhang Jun 2020

Effects Of Different Enzyme Combination And Dissociation‐Time On The Protoplast Isolation Of Alfalfa, Aiping Chen, Yupeng Luo, Run Yan, Bo Zhang

IGC Proceedings (1997-2023)

No abstract provided.


Isolation And Characterization Of Natural Protease Producers Of Bacillus Spp. From Soil Samples, Ylberinë Baliu, Bahtir Hyseni, Shkëlqim Hyseni, Aida Rushiti, Flora Ferati, Fatos Rexhepi, Rifat Morina, Sabri Hajdini, Emrah Nikerel Oct 2018

Isolation And Characterization Of Natural Protease Producers Of Bacillus Spp. From Soil Samples, Ylberinë Baliu, Bahtir Hyseni, Shkëlqim Hyseni, Aida Rushiti, Flora Ferati, Fatos Rexhepi, Rifat Morina, Sabri Hajdini, Emrah Nikerel

UBT International Conference

Proteases are group of enzymes that catalyze the hydrolysis of proteins, resulting in peptide and amino acid production. These are considered as commercially most significant among industrial enzymes, consisting about 60% of the total enzyme market. Proteases have wide areas of application in several industries like food, detergent, textile, pulp and paper, and pharmaceutical industries, as these offer environmentally benign, sustainable solutions to several challenges in the corresponding industry.Enzymes can be produced by plants, animals or microorganisms, yet the majority is produced by (recombinant) microorganisms, typically with modified features for high enzyme production. However, for e.g. food industry, the use …


Site-Directed Mutagenesis Of Malate Dehydrogenase: A Class Project, Bruce J. Heyen, Chesley Rowlett, Jon Zatorski, Ryan Burch, Emily Veach, Andy Gemmaka Apr 2018

Site-Directed Mutagenesis Of Malate Dehydrogenase: A Class Project, Bruce J. Heyen, Chesley Rowlett, Jon Zatorski, Ryan Burch, Emily Veach, Andy Gemmaka

Scholar Week 2016 - present

Malate dehydrogenase (MDH) is an important enzyme in an organism’s metabolic pathways. MDH is found in almost all living cells and catalyzes the conversion of malate to oxaloacetate which also involves nicotinamide dehydrogenase (NAD) as a coenzyme. A method to study how an enzyme operates is to alter one of its amino acids and compare the activity of the enzyme before and after the mutation. As a class project in Advanced Biochemistry during the spring semester of 2018, we are working as a team to propose and carry out a point-based mutation on MDH.


Development Of A Novel Enzymatic Pre-Treatment For Lignocellulosic Biomass, Melissa Robins, Jenna Rickus Aug 2015

Development Of A Novel Enzymatic Pre-Treatment For Lignocellulosic Biomass, Melissa Robins, Jenna Rickus

The Summer Undergraduate Research Fellowship (SURF) Symposium

Biofuels, fuels derived directly from living matter, present a renewable and environmentally friendly alternative to petroleum based fuels. Bioethanol produced from low input energy crops or agricultural waste is a promising fuel source because it does not interfere with the human food supply chain and the ethanol produced can be blended with gasoline. These potential sources of bioethanol are not yet commercially viable due to a polymer called lignin present in the plant’s cell wall which impedes the conversion of cellulose to glucose and the eventual fermentation of glucose to ethanol. Developing new methods for the pretreatment of lignocellulosic biomass …