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Full-Text Articles in Life Sciences
A Unique Mycobacterium Species Isolated From An Epizootic Of Striped Bass (Morone Saxatilis), Martha W. Rhodes, Howard Kator, Shaban Kotob, Peter Van Berkum, Ilsa Kaattari, Wolfgang K. Vogelbein, Margaret Floyd, W. Ray Butler, Frederick D. Quinn, Christopher Ottinger, Emmet Shotts
A Unique Mycobacterium Species Isolated From An Epizootic Of Striped Bass (Morone Saxatilis), Martha W. Rhodes, Howard Kator, Shaban Kotob, Peter Van Berkum, Ilsa Kaattari, Wolfgang K. Vogelbein, Margaret Floyd, W. Ray Butler, Frederick D. Quinn, Christopher Ottinger, Emmet Shotts
VIMS Articles
We isolated a Mycobacterium sp. resembling Mycobacterium marinum and M. ulcerans from diseased striped bass (Morone saxatilis) during an epizootic of mycobacteriosis in the Chesapeake Bay. This isolate may represent an undescribed Mycobacterium species, based on phenotypic characteristics and comparative 16S rRNA gene sequence.
Emerging Areas Of Research Reported During The Cdc National Conference On Pfiesteria: From Biology To Public Health, C Rubin, Eugene M. Burreson
Emerging Areas Of Research Reported During The Cdc National Conference On Pfiesteria: From Biology To Public Health, C Rubin, Eugene M. Burreson
VIMS Articles
Since its identification in 1996, the marine dinoflagellate Pfiesteria piscicida Steidinger & Burkholder has been the focus of intense scientific inquiry in disciplines ranging from estuarine ecology to epidemiology and from molecular biology to public health. Despite these research efforts, the extent of human exposure and the degree of human illness directly associated with Pfiesteria is still in the process of being defined. Unfortunately, during this same time Pfiesteria has also stimulated media coverage that in some instances jumped ahead of the science to conclude that Pfiesteria presents a widespread threat to human health. Political and economic forces also came …
Skin Ulcers In Estuarine Fishes: A Comparative Pathological Evaluation Of Wild And Laboratory-Exposed Fish, Wolfgang K. Vogelbein, Jeffrey D. Shields, Lw Haas, Kimberly S. Reece, D. E. Zwerner
Skin Ulcers In Estuarine Fishes: A Comparative Pathological Evaluation Of Wild And Laboratory-Exposed Fish, Wolfgang K. Vogelbein, Jeffrey D. Shields, Lw Haas, Kimberly S. Reece, D. E. Zwerner
VIMS Articles
The toxic dinoflagellate Pfiesteria piscicida Steidinger & Burkholder has recently been implicated as the etiologic agent of acute mass mortalities and skin ulcers in menhaden, Brevoortia tyrannus, and other fishes from mid-Atlantic U.S. estuaries. However, evidence for this association is largely circumstantial and controversial. We exposed tilapia (Oreochromis spp.) to Pfiesteria shurnwayae Glasgow & Burkholder (identification based on scanning electron microscopy and molecular analyses) and compared the resulting pathology to the so-called Pfiesteria-specific lesions occurring in wild menhaden. The tilapia challenged by high concentrations (2,000-12,000 cells/mL) of P. shurnwayae exhibited loss of mucus coat and scales plus mild petecchial hemorrhage, …
Differential Diagnosis Of Mixed Haplosporidium Costale And Haplosporidium Nelsoni Infections In The Eastern Oyster, Crassostrea Virginica, Using Dna Probes, Na Stokes, Em Burreson
Differential Diagnosis Of Mixed Haplosporidium Costale And Haplosporidium Nelsoni Infections In The Eastern Oyster, Crassostrea Virginica, Using Dna Probes, Na Stokes, Em Burreson
VIMS Articles
Haplosporidium costale and Haplosporidium nelsoni are morphologically similar pathogens of the eastern oyster Crassostrea virginica. In the absence of the spore stage, infections of the two species are extremely difficult, if not impossible, to distinguish using traditional light microscopy of stained tissue sections. Species-specific molecular diagnostics were developed for H. costale from the small subunit ribosomal DNA (SSU rDNA) sequence. The polymerase chain reaction (PCR) primers amplified a 557 base pair (bp) region of the H. costale SSU rDNA, but did not amplify DNA from oyster (C. virginica) or from six other haplosporidans (H. nelsoni, H. louisiana, H. lusitanicum, Minchinia …
A Comparative Field Study Of Crassostrea Ariakensis (Fujita 1913) And Crassostrea Virginica (Gmelin 1791) In Relation To Salinity In Virginia, G. W. Calvo, Mark Luckenbach, Standish K. Allen Jr., E. M. Burreson
A Comparative Field Study Of Crassostrea Ariakensis (Fujita 1913) And Crassostrea Virginica (Gmelin 1791) In Relation To Salinity In Virginia, G. W. Calvo, Mark Luckenbach, Standish K. Allen Jr., E. M. Burreson
VIMS Articles
We examined survival, growth, and disease susceptibility of triploid Crassostrea ariakensis (= rivularis) and compared results with that of diploid Crassostrea virginica. Two hundred and fifty oysters (age = 2 yr, mean shell height = 60-64 mm) of each species were deployed at duplicate sites, (Chesapeake Bay, and the Atlantic Coast of Virginia) within low, medium, and high salinity regimes respectively (< 15%, 15-25%, > 25%). Over the course of the study, from June 1998 to September 1999, C. virginica exhibited low survival, modest growth and high disease susceptibility. In contrast, C. ariakensis exhibited high survival, high growth rate, and low disease susceptibility. …
Development And Verification Of A Model For The Population Dynamics Of The Protistan Parasite, Perkinsus Marinus, Within Its Host, The Eastern Oyster, Crassostrea Virginica, In Chesapeake Bay, Lmr Calvo, Rl Wetzel, Em Burreson
Development And Verification Of A Model For The Population Dynamics Of The Protistan Parasite, Perkinsus Marinus, Within Its Host, The Eastern Oyster, Crassostrea Virginica, In Chesapeake Bay, Lmr Calvo, Rl Wetzel, Em Burreson
VIMS Articles
A simulation model was developed to investigate the population dynamics of the protistan parasite, Perkinsus marinus, within its host, the eastern oyster, Crassostrea virginica. The main objective was to evaluate the relationship between P. marinus population dynamics and environmental conditions in order to predict the onset and termination of P. marinus epizootics in Chesapeake Bay oyster populations. Information derived from laboratory experiments and from direct field observations of P. marinus dynamics in the James River for the years 1990 to 1993 was utilized for model development. The individual-based model, which is driven by temperature and salinity, tracks the average within-host …
Reduction In Dna Binding Activity Of The Transcription Factor Pax-5a In B Lymphocytes Of Aged Mice, J Anspach, G Poulsen, I Kaattari, R Pollock, P Zwollo
Reduction In Dna Binding Activity Of The Transcription Factor Pax-5a In B Lymphocytes Of Aged Mice, J Anspach, G Poulsen, I Kaattari, R Pollock, P Zwollo
VIMS Articles
Aging has been associated with intrinsic changes of the humoral immune response, which may lead to an increased occurrence of autoimmune disorders and pathogenic susceptibility. The transcription factor Pax-5 is a key regulator of B cell development. Pax-5a/B cell-specific activator protein and an alternatively spliced isoform, Pax-Sd, may have opposing functions in transcriptional regulation due to the lack of a transactivation domain in Pax-Sd. To study B cell-specific changes that occur during the aging process, we investigated expression patterns of Pax-Sa and Sd in mature B cells of young and aged mice. RNase protection assays showed a similar transcriptional pattern …