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Full-Text Articles in Life Sciences

Sorbitol-Fermenting Bifidobacteria As Indicators Of Diffuse Human Faecal Pollution In Estuarine Waters, Mw Rhodes, H Kator Oct 1999

Sorbitol-Fermenting Bifidobacteria As Indicators Of Diffuse Human Faecal Pollution In Estuarine Waters, Mw Rhodes, H Kator

VIMS Articles

Sorbitol fermenting bifidobacteria were evaluated as indicators of non-point source human faecal pollution to three sub-estuaries with elevated faecal coliform densities. Human-specific bifidobacteria correlated with identifiable human sanitary deficiencies in feeder streams to estuarine creeks in two of three watersheds examined, one rural and one moderately developed. Sorbitol-fermenting bifidobacteria were recovered at densities ranging from 1 to 90 colony-forming-units 100 ml(-1) in 11 of 258 water samples but were undetected in sediment (n = 68) and scat from resident wildlife (deer, muskrat and raccoon, n = 20). Failure to detect sorbitol-fermenting bifidobacteria in water samples during the summer months was …


Perkinsus Marinus Extracellular Protease Modulates Survival Of Vibrio Vulnificus In Eastern Oyster (Crassostrea Virginica) Hemocytes, Bd Tall, Jf La Peyre, Et Al, M Faisal Sep 1999

Perkinsus Marinus Extracellular Protease Modulates Survival Of Vibrio Vulnificus In Eastern Oyster (Crassostrea Virginica) Hemocytes, Bd Tall, Jf La Peyre, Et Al, M Faisal

VIMS Articles

The in vitro effects of the Perkinsus marinus serine protease on the intracellular survival of Vibrio vulnificus in oyster hemocytes were examined by using a time-course gentamicin internalization assay. Results showed that protease-treated hemocytes were initially slower to internalize V. vulnificus than untreated hemocytes. After 1 h, the elimination of V. vulnificus by treated hemocytes was significantly suppressed compared with hemocytes infected with invasive and noninvasive controls. Our data suggest that the serine protease produced by P. marinas suppresses the vibriocidal activity of oyster hemocytes to effectively eliminate V. vulnificus, potentially leading to conditions favoring higher numbers of vibrios in …


Estimation Of Bacterial Respiration And Growth Efficiency In The Ross Sea, Antarctica, C. A. Carlson, N. R. Bates, H. W. Ducklow, D. A. Hansell Jan 1999

Estimation Of Bacterial Respiration And Growth Efficiency In The Ross Sea, Antarctica, C. A. Carlson, N. R. Bates, H. W. Ducklow, D. A. Hansell

VIMS Articles

Seawater cultures were conducted in large volume (36 l) gas impermeable tri-laminate bags for the purpose of empirically deriving bacterial growth efficiency (BGE) and carbon conversion factors (CCF) in the south central Ross Sea. This experimental design allowed for concomitant measurements of metabolic reactants (loss of total and dissolved organic carbon [TOC and DOG]) and products (gain of total carbon dioxide [TCO2] and bacterial biomass) to be made from a single incubation vessel. Some previous studies have relied on proxy measurements (e.g. O-2, H-3-thymidine incorporation and cell abundance) to determine BGE and CCF rather than direct carbon measurements. Our experimental …


Bacterial Growth In Experimental Plankton Assemblages And Seawater Cultures From The Phaeocystis Antarctica Bloom In The Ross Sea, Antarctica, H. W. Ducklow, C. Carlson, Walker O. Smith Jr. Jan 1999

Bacterial Growth In Experimental Plankton Assemblages And Seawater Cultures From The Phaeocystis Antarctica Bloom In The Ross Sea, Antarctica, H. W. Ducklow, C. Carlson, Walker O. Smith Jr.

VIMS Articles

A series of seawater culture experiments was carried out during the Phaeocystis antarctica bloom in the Ross Sea polynya (76.5 degrees S, 180 degrees W; November to December 1994 and December 1995 to January 1996) to examine bacterioplankton growth and derive empirical factors for estimating bacterial production rates. Bacterial growth was exponential over 3 to 10 d in all experiments, at rates of ca 0.1 to 0.7 d(-1), even in persistently cold waters (-2 to + 1 degrees C). Growth rates were lower in the early part of the bloom (early to mid-November) and highest during the period of peak …


The Bacterial Component Of The Oceanic Euphotic Zone, H. W. Ducklow Jan 1999

The Bacterial Component Of The Oceanic Euphotic Zone, H. W. Ducklow

VIMS Articles

Bacteria in the open sea remote from land are sustained strictly on local sources of organic production which should make understanding their nutrition and growth regulation easier than in nearshore systems, estuaries and lakes. Until now, a paucity of data from geographically isolated oceanic sites prevented ready :interpretation. In the past decade investigation of bacterial properties in oceanic systems has increased rapidly, stimulated in part by large oceanographic programs like the Joint Global Ocean Flux Study. Here I review comprehensive investigations of bacterial biomass and production dynamics in the subarctic north Atlantic and north Pacific, oligotrophic gyres in both oceans, …