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VIMS Articles

1999

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Articles 1 - 26 of 26

Full-Text Articles in Life Sciences

Sorbitol-Fermenting Bifidobacteria As Indicators Of Diffuse Human Faecal Pollution In Estuarine Waters, Mw Rhodes, H Kator Oct 1999

Sorbitol-Fermenting Bifidobacteria As Indicators Of Diffuse Human Faecal Pollution In Estuarine Waters, Mw Rhodes, H Kator

VIMS Articles

Sorbitol fermenting bifidobacteria were evaluated as indicators of non-point source human faecal pollution to three sub-estuaries with elevated faecal coliform densities. Human-specific bifidobacteria correlated with identifiable human sanitary deficiencies in feeder streams to estuarine creeks in two of three watersheds examined, one rural and one moderately developed. Sorbitol-fermenting bifidobacteria were recovered at densities ranging from 1 to 90 colony-forming-units 100 ml(-1) in 11 of 258 water samples but were undetected in sediment (n = 68) and scat from resident wildlife (deer, muskrat and raccoon, n = 20). Failure to detect sorbitol-fermenting bifidobacteria in water samples during the summer months was consistent ...


Perkinsus Marinus Extracellular Protease Modulates Survival Of Vibrio Vulnificus In Eastern Oyster (Crassostrea Virginica) Hemocytes, Bd Tall, Jf La Peyre, Et Al, M Faisal Sep 1999

Perkinsus Marinus Extracellular Protease Modulates Survival Of Vibrio Vulnificus In Eastern Oyster (Crassostrea Virginica) Hemocytes, Bd Tall, Jf La Peyre, Et Al, M Faisal

VIMS Articles

The in vitro effects of the Perkinsus marinus serine protease on the intracellular survival of Vibrio vulnificus in oyster hemocytes were examined by using a time-course gentamicin internalization assay. Results showed that protease-treated hemocytes were initially slower to internalize V. vulnificus than untreated hemocytes. After 1 h, the elimination of V. vulnificus by treated hemocytes was significantly suppressed compared with hemocytes infected with invasive and noninvasive controls. Our data suggest that the serine protease produced by P. marinas suppresses the vibriocidal activity of oyster hemocytes to effectively eliminate V. vulnificus, potentially leading to conditions favoring higher numbers of vibrios in ...


Fish Species Richness In Relation To Restored Oyster Reefs, Piankatank River, Virginia, Jm Harding, Roger L. Mann Jun 1999

Fish Species Richness In Relation To Restored Oyster Reefs, Piankatank River, Virginia, Jm Harding, Roger L. Mann

VIMS Articles

Fish assemblages in relation to “reef” structures in marine habitats have been and continue to be topics for research addressing ecological and management questions. Much effort has been spent describing and defining fish assemblages, or groups of species, associated with tropical coral reefs (e.g., Sale 1991 and chapters therein), temperate hard bottom or rocky reefs (e.g., Sedberry and Van Dolah, 1984; Ambrose and Swarbrick, 1989), tropical lava flows (e.g., Godwin and Kosaki, 1989), and artificial “fishing” reefs (e.g., Chandler et al., 1985; Hueckel and Buckley, 1987; Bohnsack, 1989; Feigenbaum et al., 1989; Rountree, 1989; Stephan and ...


Antigenic And Functional Characterization Of P57 Produced By Renibacterium Salmoninarum, Gd Wiens, Ms Chien, Jr Winton, Sl Kaattari May 1999

Antigenic And Functional Characterization Of P57 Produced By Renibacterium Salmoninarum, Gd Wiens, Ms Chien, Jr Winton, Sl Kaattari

VIMS Articles

Renibacterium salmoninarum, the causative agent of bacterial kidney disease, produces large quantities of a 57-58 kDa protein (p57) during growth in broth culture and during infection of salmonid fish. Biological activities of secreted p57 include agglutination of salmonid leucocytes and rabbit erythrocytes. We define the location of epitopes on p57 recognized by agglutination-blocking monoclonal antibodies (MAbs) 4C11, 4H8 and 4D3, and demonstrate that the majority of secreted p57 is a monomer that retains salmonid leucocyte agglutinating activity. The 3 MAbs bound a recombinant, amino-terminal fragment of p57 (211 aa) but not a carboxy-terminal fragment (315 aa) demonstrating that the neutralizing ...


Elevated Temperature Treatment As A Novel Method For Decreasing P57 On The Cell Surface Of Renibacterium Salmoninarum, Jon D. Piganelli, Gregory D. Weins, Stephen L. Kaattari Apr 1999

Elevated Temperature Treatment As A Novel Method For Decreasing P57 On The Cell Surface Of Renibacterium Salmoninarum, Jon D. Piganelli, Gregory D. Weins, Stephen L. Kaattari

VIMS Articles

Renibacterium salmoninarum is a Gram-positive diplo-bacillus and the causative agent of bacterial kidney disease, a prevalent disease of salmonid fish. Virulent isolates of R. salmoninarum have a hydrophobic cell surface and express the 57-58 kDa protein (p57). Here we have investigated parameters which effect cell hydrophobicity and p57 degradation. Incubation of R, salmoninarum cells at 37 degrees C for >4 h decreased cell surface hydrophobicity as measured by the salt aggregation assay, and decreased the amount of cell associated p57. Incubation of cells at lower temperatures (22, 17, 4 or -20 degrees C) for up to 16 h did not ...


Non-Redfield Carbon And Nitrogen Cycling In The Arctic: Effects Of Ecosystem Structure And Dynamics, Kendra L. Daly, Douglas W.R. Wallace, Walker O. Smith Jr., Annelie Skoog, Ruben Lara, Michel Gosselin, Eva Falck, Patricia L. Yager Feb 1999

Non-Redfield Carbon And Nitrogen Cycling In The Arctic: Effects Of Ecosystem Structure And Dynamics, Kendra L. Daly, Douglas W.R. Wallace, Walker O. Smith Jr., Annelie Skoog, Ruben Lara, Michel Gosselin, Eva Falck, Patricia L. Yager

VIMS Articles

The C:N ratio is a critical parameter used in both global ocean carbon models and field studies to understand carbon and nutrient cycling as well as to estimate exported carbon from the euphotic zone. The so-called Redfield ratio (C:N = 6.6 by atoms) [Redfield et nl., 1963] is widely used for such calculations. Here we present data from the NE Greenland continental shelf that show that most of the C:N ratios for particulate (autotrophic and heterotrophic) and dissolved pools and rates of transformation among them exceed Redfield proportions from June to August, owing to species composition, size ...


Observations On The Biology Of The Veined Rapa Whelk, Rapana Venosa (Valenciennes, 1846) In The Chesapeake Bay, Juliana Harding, Roger L. Mann Jan 1999

Observations On The Biology Of The Veined Rapa Whelk, Rapana Venosa (Valenciennes, 1846) In The Chesapeake Bay, Juliana Harding, Roger L. Mann

VIMS Articles

The recent discovery of the Veined Rapa whelk (Rapana venosa, Valenciennes, 1846) in the lower Chesapeake Bay provides an opportunity to observe the initial biological and ecological consequences of a novel bioinvasion. These large predatory gastropods occur in subtidal, hard bottom habitats in the lower Bay and are capable of feeding, mating, and moving while completely burrowed. Hard clams (Mercenaria mercenaria) are consumed preferentially in the laboratory when offered concurrently with oysters (Crassostrea virginica), soft clams (Mya arenaria), and mussels (Mytilus edulis). Chesapeake Bay R. venosa readily open and consume large hard clams (30 to 85 mm SH) leaving no ...


Comparative Field Study Of Crassostrea Gigas (Thunberg, 1793) And Crassostrea Virginica (Gmelin, 1791) In Relation To Salinity In Virginia, Gustavo W. Calvo, Mark Luckenbach, Standish K. Allen, Eugene Burreson Jan 1999

Comparative Field Study Of Crassostrea Gigas (Thunberg, 1793) And Crassostrea Virginica (Gmelin, 1791) In Relation To Salinity In Virginia, Gustavo W. Calvo, Mark Luckenbach, Standish K. Allen, Eugene Burreson

VIMS Articles

To evaluate and compare the performance of triploid juvenile C. gigas (mean shell height = 19.2 mm) and triploid juvenile Crassostrea virginica (mean shell height = 31.7 mm), 600 oysters of each species were deployed for 1 year in floating mesh cages at three replicate sites within low, medium, and high salinity regimes (respectively,25%) in the Chesapeake Bay and the Atlantic Coast of Virginia. The comparative performance of the two oyster species varied with salinity. At low salinity sites, cumulative mortality of C. virginica (10%) was significantly (P < .05) lower than that of C. gigas (63%), and over-all mean growth rate of C. virginica (2.9 mm mo(-1)) was significantly (P < .05) higher than that of C. gigas (1.6 mm mo(-1)). At medium salinity sites, survival and growth rate of C. virginica and C. gigas were nor significantly (P > .05) different. Both species experienced moderately high cumulative mortality at the ...


Morphometric Separation Of Annual Cohorts Within Mid-Atlantic Bluefish, Pomatomus Saltatrix, Using Discriminant Function Analysis, Herbert Austin, Daniel Scoles, Allison J. Abell Jan 1999

Morphometric Separation Of Annual Cohorts Within Mid-Atlantic Bluefish, Pomatomus Saltatrix, Using Discriminant Function Analysis, Herbert Austin, Daniel Scoles, Allison J. Abell

VIMS Articles

Larval and tagging studies neither support nor refute the existence of two mid-Atlantic stocks of bluefish, Pomatomus saltatrix, one that spawns just south of Hatteras, North Carolina, in the spring, and the other off New England in the summer. Subsequent studies have suggested either a continuous spawning with two survival events, or two distinct spawning events, one in spring south of Hatteras, NC, and a second in mid-summer from Long Island to New England. Genetic studies have supported a single stock because they show the existence of a single gene pool. A stepwise linear discriminant function analysis (DFA) of morphometrics ...


Age, Growth, Longevity, And Mortality Of Blackcheek Tonguefish, Symphurus Plagiusa (Cynoglossidae : Pleuronectiformes), In Chesapeake Bay, Virginia, Mark R. Terwilliger, Thomas A. Munroe Jan 1999

Age, Growth, Longevity, And Mortality Of Blackcheek Tonguefish, Symphurus Plagiusa (Cynoglossidae : Pleuronectiformes), In Chesapeake Bay, Virginia, Mark R. Terwilliger, Thomas A. Munroe

VIMS Articles

Age structure, longevity, and mortality were determined for a population of blackcheek tonguefish, Symphurus plagiusa, in Chesapeake Bay. Blackcheek tonguefish (36-202 mm TL) were randomly collected by means of otter trawl in lower Chesapeake Bay and major Virginia tributaries (James, York, and Rappahannock rivers) from April 1994 through August 1995. Ages were determined by interpreting growth increments on thin transverse sections of sagittal otoliths. Marginal increment analysis showed that a single annulus was formed in June of each year. Blackcheek tonguefish caught during this study reached a maximum age of 5+ years. Growth was rapid during the first year, then ...


Ontogenetic Changes In Habitat Use By Postlarvae And Young Juveniles Of The Blue Crab, Ra Pardieck, R J. Orth, Rj Diaz, Rom Lipcius Jan 1999

Ontogenetic Changes In Habitat Use By Postlarvae And Young Juveniles Of The Blue Crab, Ra Pardieck, R J. Orth, Rj Diaz, Rom Lipcius

VIMS Articles

Changing habitat requirements are evident during the developmental cycles of many species. In this field investigation, we attempted to distinguish between depth (shallow vs deep), habitat structure (seagrass species), and study site as factors influencing the distribution and abundance of postlarvae and juvenile blue crabs Callinectes sapidus in the Chesapeake Bay. Deep (greater than or equal to 70 cm mean low water [MLW]) and shallow (less than or equal to 50 cm MLW) suction samples in monospecific Zostera marina and Ruppia maritima beds were taken in the York River, a tributary of the Chesapeake Bay. Our studies revealed ontogenetic changes ...


Abundance And Vertical Distribution Of Drifting, Post-Larval Macoma Spp. (Bivalvia : Tellinidae) In The York River, Virginia, Usa, Lp Garrison, Ja Morgan Jan 1999

Abundance And Vertical Distribution Of Drifting, Post-Larval Macoma Spp. (Bivalvia : Tellinidae) In The York River, Virginia, Usa, Lp Garrison, Ja Morgan

VIMS Articles

We sampled the early drifting post-larvae of a complex of 2 species of tellinid bivalves, Macoma spp., at a station in the lower York River, Chesapeake Bay, USA. Plankton samples were collected by pump every 3 h from 3 depths (surface, mid-depth, and bottom) on 4 dates corresponding to full and new moons. Macoma spp. post-larvae (size range 400 to 500 mu m) were abundant in the plank ton throughout the sampling period. The environmental factors influencing the abundance and vertical distribution of drifting post-larvae were evaluated using linear and logistic regression. Post-larvae were always more abundant during night as ...


Selective Feeding Behavior Of Larval Naked Gobies Gobiosoma Bosc And Blennies Chasmodes Bosquianus And Hypsoblennius Hentzi: Preferences For Bivalve Veligers, Jm Harding Jan 1999

Selective Feeding Behavior Of Larval Naked Gobies Gobiosoma Bosc And Blennies Chasmodes Bosquianus And Hypsoblennius Hentzi: Preferences For Bivalve Veligers, Jm Harding

VIMS Articles

Naked gobies Gobiosoma bose, striped blennies Chasmodes bosquianus, and feather blennies Hypsoblennius hentzi provide important intermediate links within the trophic structure of estuarine oyster reef communities. Predator-prey interactions between planktonic larvae of these fishes and larval eastern oysters Crassostrea virginica may influence recruitment success within oyster reef communities. These 3 species of oyster reef fish larvae were cultured from wild nests and used in multifactorial laboratory feeding experiments with larval oysters or hard clams Mercenaria mercenaria as well as wild plankton as prey items to determine the effects of predator age, predator concentration, and prey type on feeding selectivity of ...


Correspondence Between Environmental Gradients And Summer Littoral Fish Assemblages In Low Salinity Reaches Of The Chesapeake Bay, Usa, Cm Wagner, Hm Austin Jan 1999

Correspondence Between Environmental Gradients And Summer Littoral Fish Assemblages In Low Salinity Reaches Of The Chesapeake Bay, Usa, Cm Wagner, Hm Austin

VIMS Articles

Patterns in the assemblage structure of littoral fishes occupying the gradient between riverine and estuarine ecosystems were revealed through multivariate analysis of 5 annual summer seine surveys in 4 tributary systems of the lower Chesapeake Bay. Catch per unit effort of fishes was quantified and environmental variables measured to characterize assemblage structure and population responses along large-scale (km) environmental gradients. Results of two-way indicator species analysis (TWINSPAN), detrended correspondence analysis (DCA) and detrended canonical correspondence analysis (DCCA) suggested the presence of 4 intergrading assemblages of littoral beach fishes: permanent tidal freshwater, lower tidal freshwater, oligohaline estuary and mesohaline estuary. Littoral ...


Vertical Migration Behavior And Larval Transport In Brachyuran Crabs, Lp Garrison Jan 1999

Vertical Migration Behavior And Larval Transport In Brachyuran Crabs, Lp Garrison

VIMS Articles

I documented the patterns of vertical distribution and abundance of zoeae from 3 families of brachyuran crabs in the York River, a sub-estuary in southern Chesapeake Bay (USA). The family groups included species from Ocypodidae, Pinnotheridae, and Panopeidae. I assessed the effects of Light and tidal current stage on the abundance and vertical distribution of zoeae using analysis of variance (ANOVA) and logistic regression. Clear behavioral patterns were evident in the logistic regression. Ocypodid larvae exhibited tidally timed behavior with larvae nearest the surface during ebb tides. Pinnotherid larval behaviors were correlated with both light and tidal current phase. Larvae ...


Flow Convergence And Stability At A Tidal Estuarine Front: Acoustic Doppler Current Observations, John M. Brubaker, John H. Simpson Jan 1999

Flow Convergence And Stability At A Tidal Estuarine Front: Acoustic Doppler Current Observations, John M. Brubaker, John H. Simpson

VIMS Articles

Characteristics of the flow field in an estuarine frontal zone have been investigated in a field study in the lower James River estuary. Underway sampling with an acoustic Doppler current profiler (ADCP) on repeated transects across the front provided information on the structure of the flow field near the front and its evolution in time. As this tidal intrusion front advanced up the estuary during the flooding tide, prominent and consistent features in the velocity field included a localized zone of convergent flow beneath the visible surface line and a stratified shear layer just upriver of the front. Within the ...


The Bacterial Component Of The Oceanic Euphotic Zone, H. W. Ducklow Jan 1999

The Bacterial Component Of The Oceanic Euphotic Zone, H. W. Ducklow

VIMS Articles

Bacteria in the open sea remote from land are sustained strictly on local sources of organic production which should make understanding their nutrition and growth regulation easier than in nearshore systems, estuaries and lakes. Until now, a paucity of data from geographically isolated oceanic sites prevented ready :interpretation. In the past decade investigation of bacterial properties in oceanic systems has increased rapidly, stimulated in part by large oceanographic programs like the Joint Global Ocean Flux Study. Here I review comprehensive investigations of bacterial biomass and production dynamics in the subarctic north Atlantic and north Pacific, oligotrophic gyres in both oceans ...


Estimation Of Bacterial Respiration And Growth Efficiency In The Ross Sea, Antarctica, C. A. Carlson, N. R. Bates, H. W. Ducklow, D. A. Hansell Jan 1999

Estimation Of Bacterial Respiration And Growth Efficiency In The Ross Sea, Antarctica, C. A. Carlson, N. R. Bates, H. W. Ducklow, D. A. Hansell

VIMS Articles

Seawater cultures were conducted in large volume (36 l) gas impermeable tri-laminate bags for the purpose of empirically deriving bacterial growth efficiency (BGE) and carbon conversion factors (CCF) in the south central Ross Sea. This experimental design allowed for concomitant measurements of metabolic reactants (loss of total and dissolved organic carbon [TOC and DOG]) and products (gain of total carbon dioxide [TCO2] and bacterial biomass) to be made from a single incubation vessel. Some previous studies have relied on proxy measurements (e.g. O-2, H-3-thymidine incorporation and cell abundance) to determine BGE and CCF rather than direct carbon measurements. Our ...


Bacterial Growth In Experimental Plankton Assemblages And Seawater Cultures From The Phaeocystis Antarctica Bloom In The Ross Sea, Antarctica, H. W. Ducklow, C. Carlson, W. Smith Jan 1999

Bacterial Growth In Experimental Plankton Assemblages And Seawater Cultures From The Phaeocystis Antarctica Bloom In The Ross Sea, Antarctica, H. W. Ducklow, C. Carlson, W. Smith

VIMS Articles

A series of seawater culture experiments was carried out during the Phaeocystis antarctica bloom in the Ross Sea polynya (76.5 degrees S, 180 degrees W; November to December 1994 and December 1995 to January 1996) to examine bacterioplankton growth and derive empirical factors for estimating bacterial production rates. Bacterial growth was exponential over 3 to 10 d in all experiments, at rates of ca 0.1 to 0.7 d(-1), even in persistently cold waters (-2 to + 1 degrees C). Growth rates were lower in the early part of the bloom (early to mid-November) and highest during the ...


Phytoplankton Growth Rates In The Ross Sea, Antarctica, Determined By Independent Methods: Temporal Variations, Walker O. Smith Jr., D. M. Nelson, S. Mathot Jan 1999

Phytoplankton Growth Rates In The Ross Sea, Antarctica, Determined By Independent Methods: Temporal Variations, Walker O. Smith Jr., D. M. Nelson, S. Mathot

VIMS Articles

The development of the seasonal phytoplankton bloom in the Ross Sea was studied during two cruises. The first, conducted in November-December 1994, investigated the initiation and rapid growth of the bloom, whereas the second (December 1995-January 1996) concentrated on the bloom's maximum biomass period and the subsequent decline in biomass. Central to the understanding of the controls of growth and the summer decline of the bloom is a quantitative assessment of the growth rate of phytoplankton. Growth rates were estimated over two time scales with different methods. The first estimated daily growth rates from isotopic incorporation under simulated in ...


Phylogenetic Analysis Of Cytochrome C Oxidase I Sequences To Determine Higher-Level Relationships Within The Coleoid Cephalopods, Db Carlin, Je Graves Jan 1999

Phylogenetic Analysis Of Cytochrome C Oxidase I Sequences To Determine Higher-Level Relationships Within The Coleoid Cephalopods, Db Carlin, Je Graves

VIMS Articles

Until recently, the higher-level phylogenetic relationships of coleoid cephalopods have remained unclear. A thorough knowledge of the higher-level phylogeny of the group has been limited by the paucity of paleontological data for this poorly-fossilized group and the lack of cladistic analyses of developmental, morphological, and molecular data applied to the coleoids. In this study we analyzed a 657 base pair portion of the mitochondrial cytochrome c oxidase I (COI) gene from 48 cephalopod species representing a broad spectrum of coleoid diversity to examine higher-level phylogenetic relationships within the group. The COI gene exhibited a high degree of nucleotide sequence variability ...


Use Of Molecular Tools For Mollusc Disease Diagnosis, Fcj Berthe, Em Burreson, M Hine Jan 1999

Use Of Molecular Tools For Mollusc Disease Diagnosis, Fcj Berthe, Em Burreson, M Hine

VIMS Articles

Recently, an increasing number of research teams arc engaged in developing DNA-based diagnostic techniques for mollusc pathogens. These techniques are slowly moving from development in specialised laboratories for research purposes, to routine application and are expected to find an increasing use in routine disease monitoring programs in mollusc aquaculture and in efforts to prevent the spread of pathogens within and between nations. Considering the probable development and interest in these diagnostic techniques, the potential for wide applications in aquaculture and the inherent problems currently associated with their use, it appears necessary to address these issues.


Potential Role Of Protease-Antiprotease Interactions In Perkinsus Marinus Infection In Crassostrea Spp., M Faisal, Jl Oliver, Sl Kaataari Jan 1999

Potential Role Of Protease-Antiprotease Interactions In Perkinsus Marinus Infection In Crassostrea Spp., M Faisal, Jl Oliver, Sl Kaataari

VIMS Articles

Perkinsus marinus causes devastating losses in populations of the eastern oyster (Crassostrea virginica). Our studies have demonstrated that P. marinus secretes extracellular serine proteases which enhance parasite propagation and compromise host defences. Crassostrea virginica. however, possesses several inhibitors of these proteases. The Pacific oyster (C. gigas) is resistant to P. marinus and possesses protease inhibitors with significantly higher specific activities than those in C. virginica. Interestingly, Crassostrea spp. themselves, elaborate metalloprotease activities which can be detected in their plasma, and are increased during P. marinus infections. Together our work suggests that there may be a broad spectrum of humoral host ...


Environmental Factors And The Infectious Disease Caused By The Protozoan Parasite, Perkinsus Marinus, In Eastern Oysters (Crassostrea Virginica), Fle Chu Jan 1999

Environmental Factors And The Infectious Disease Caused By The Protozoan Parasite, Perkinsus Marinus, In Eastern Oysters (Crassostrea Virginica), Fle Chu

VIMS Articles

Temperature and salinity are two important factors limiting the distribution and abundance of Perkinsus marinus, a protozoan parasite of Eastern oysters (Crassostrea virginica). Results of laboratory studies ale consistent with field observations and clearly demonstrate that P. marinus susceptibility and disease advancement are positively correlated with temperature, salinity and in situ number of infective cells. Laboratory findings also suggest that environmental degradation may enhance the epizootic, although disease caused by P. marinus in oysters is known to be predominantly exacerbated by elevated temperature and salinity. Oysters cellular defence mechanisms appear ineffective in defence against P. marinus. Also, pollutant esposure caused ...


Teleost Antibody Structure: Simple Prototype Or Elegant Alternative?, Sl Kaattari, Jv Klemer, Da Evans Jan 1999

Teleost Antibody Structure: Simple Prototype Or Elegant Alternative?, Sl Kaattari, Jv Klemer, Da Evans

VIMS Articles

Teleosts possess mechanism(s) by which they can generate considerable structural diversity within their tetrameric antibody molecules. In salmonids, this diversity is generated through a process of random polymerisation of the constituent monomeric subunits rather than dependency upon isotypic gene diversity. Thus, one gene product can give rise to as many as six different structural forms of immunoglobulin. In contrast to mammals, evidence suggests that this polymerisation process occurs late in the secretory process and not within the endoplasmic reticulum. This assembly process is likely to be important in the generation of teleost antibody functional diversity, thereby potentially simulating isotypy.


Isolation Of Aphanomyces Sp(P). Associated With Skin Lesions And Mortalities In The Striped (Mugil Cephalus) And The Thin Lip (Liza Ramada) Grey Mullets, Aa Shaheen, E Elsayed, M Faisal Jan 1999

Isolation Of Aphanomyces Sp(P). Associated With Skin Lesions And Mortalities In The Striped (Mugil Cephalus) And The Thin Lip (Liza Ramada) Grey Mullets, Aa Shaheen, E Elsayed, M Faisal

VIMS Articles

Aphanomyces spp. were isolated from skin erosions and ulcers of cultured Striped and thin lip grey mullets. Fungi with non-septated hyphae were found in wet mount preparations of skin. Samples inoculated on glucose peptone liquid medium for three successive steps then mycelial growths were transferred into glucose peptone yeast extract agar medium. Aphanomyces spp. were successfully isolated, in axenic cultures, from seven out of 35 fish samples. The cultures were maintained on glucose peptone yeast extract liquid media. Formation of sporangia in tap water that contained hemp seeds confirmed that the grey mullet isolates are members of tlx genus Aphanomyces ...