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Full-Text Articles in Life Sciences
Discerning Autotrophy, Mixotrophy And Heterotrophyin Marine Tack Archaea From The North Atlantic, L M. Seyler, L. R. Mcguinness, J. A. Gilbert, J. F. Biddle, Donglai Gong, L. J. Kerkhof
Discerning Autotrophy, Mixotrophy And Heterotrophyin Marine Tack Archaea From The North Atlantic, L M. Seyler, L. R. Mcguinness, J. A. Gilbert, J. F. Biddle, Donglai Gong, L. J. Kerkhof
VIMS Articles
DNA stable isotope probing (SIP) was used to track the uptake of organic and inorganic carbon sources for TACK archaea(Thaumarchaeota/Aigarchaeota/Crenarchaeota/Korarchaeota) on a cruise of opportunity in the North Atlantic. Due to water limitations, duplicate samples from the deep photic (60–115 m), the mesopelagic zones (local oxygen minimum; 215–835 m)and the bathypelagic zone (2085–2835 m) were amended with various combinations of12C- or13C-acetate/urea/bicarbonate to assess cellular carbon acquisition. The SIP results indicated the majority of TACK archaeal operational taxonomic units(OTUs) incorporated13C from acetate and/or urea into newly synthesized DNA within 48 h. A small fraction (16%) of the OTUs, often representing the …