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Articles 1 - 20 of 20
Full-Text Articles in Life Sciences
Feelings Of Connectedness To Nature: A Comparison Of Park & Recreation Management Students And Sport Management Students., N. E. Scarborough
Feelings Of Connectedness To Nature: A Comparison Of Park & Recreation Management Students And Sport Management Students., N. E. Scarborough
Undergraduate Honors Theses
In the face of the present consumer-based environmental movement, leaders in many industries and disciplines are striving to understand why people are “going green” in order to market to them. Researchers have been studying ways in which people connect to the environmental movement, and researchers in the field of sport and recreation are also conducting such studies. Several surveys have been developed to measure these connections; one such survey instrument is the Connectedness to Nature Scale (CNS). In the present study, the CNS was re-created online, and a link to the survey was sent via email to students studying Park …
Optimization Of Protocols For Wolbachia Detection Using Pcr Methods, Jennifer Kim
Optimization Of Protocols For Wolbachia Detection Using Pcr Methods, Jennifer Kim
Undergraduate Honors Theses
Wolbachia is a genus of intracellular bacterium that is known to infect a wide range of arthropods. Species within this clade have the ability to modify the reproductive success of their hosts to promote their own distribution throughout host populations. Wolbachia cannot be cultured outside of the host and characterizing infection of gonadal cells might be time consuming. Thus, polymerase chain reaction (PCR) assays are a common method of Wolbachia DNA amplification from arthropods. I assessed the utility of 3 DNA sequence markers (16S, ITS, and 28S) paired with the Wolbachia surface protein (wsp) gene in various PCR …
Leaf Anatomy And Systematics Of Polygaleae (Polygalaceae), Heather Wilber
Leaf Anatomy And Systematics Of Polygaleae (Polygalaceae), Heather Wilber
Undergraduate Honors Theses
Polygalaceae are comprised of approximately 1000 species found worldwide in a variety of habitats. Over half of the species in the family were traditionally grouped into the polyphyletic genus Polygala and need resolution. Leaves from 21 species in 13 genera within tribe Polygaleae were field collected or taken from specimens at the Missouri Botanical Garden Herbarium (MO) to find phylogenetically useful characteristics of the foliar anatomy. The leaves were rehydrated using concentrated ammonium hydroxide, embedded in paraffin, and sectioned at 10 μm thickness using a rotary microtome. To observe epidermal features, rehydrated leaves were also cleared using 3% potassium hydroxide. …
Comparison Of Theophylline And 8-Cyclopentyltheophylline On The Heart Rates Of Neonatal Rats, Stephanie Arnold
Comparison Of Theophylline And 8-Cyclopentyltheophylline On The Heart Rates Of Neonatal Rats, Stephanie Arnold
Undergraduate Honors Theses
Neonatal apnea is a serious condition that affects the health of infants, especially those born prematurely. To stimulate breathing, methylxanthine drugs, such as theophylline and caffeine, are administered to reverse this condition in humans and avoid the use of breathing-assistance equipment. These drugs cause stimulation of the central nervous system and the heart, however, both detrimental side effects in preterm infants. Antagonism of adenosine receptors is the proposed mechanism of methylxanthine action. Synthetic chemists have produced xanthine analogs with increased affinity for adenosine receptors that could lead to respiratory stimulation while reducing cardiostimulant effects. For this study, theophylline and 8-cyclopentyltheophylline …
Proximity-Labeling Of Near Neighbors Of Lamin A And Lamin A-Δ50 (Progerin)., Mohammad Sabri
Proximity-Labeling Of Near Neighbors Of Lamin A And Lamin A-Δ50 (Progerin)., Mohammad Sabri
Undergraduate Honors Theses
In an attempt to isolate and identify proteins that differentially interact with or locate near lamin A and progerin, we used a previously described method named BioID (proximity-dependent biotin identification). This method is based on fusion of a promiscuous E. coli biotin-protein ligase (BL) to a targeting protein (in this study, lamin A-GFP and progerin-GFP). The biotin ligase biotinylates amino residues in proteins that are near-neighbors of the fusion protein. To create the fusion proteins, BL was sub-cloned from a pcDNA3.1 MCS-BirA(R118G)-HA plasmid donated by Kyle Roux from University of South Dakota. The BL fragment was ligated into a pNEBR-X1-lamin …
Antioxidants And Wound Healing., Mary Jane Underdown
Antioxidants And Wound Healing., Mary Jane Underdown
Undergraduate Honors Theses
Neutrophils and cytokines present during an inflammatory response produce oxidants, such as reactive oxygen species (ROS) or reactive nitrogen species (RNS). These oxidants act as free radicals, a highly reactive species that steal electrons from nearby molecules to satisfy their valence electron needs. The removal of electrons by free radicals produces damage within the healthy cells of tissue. Antioxidants can be used to reduce this oxidative stress and reestablish the necessary environment for wound healing by donating electrons to the free radicals, sparing the damaging effects oxidation causes to other molecules. The standard procedure for administering supplemental antioxidants is through …
The Relationship Of Strength (Ipf(N), Ipfkg) Compared To Running Endurance (Vo2 And Time To Exhaustion) In Recreationally Competitive Athletes., Jonathan Dale Blankenship
The Relationship Of Strength (Ipf(N), Ipfkg) Compared To Running Endurance (Vo2 And Time To Exhaustion) In Recreationally Competitive Athletes., Jonathan Dale Blankenship
Undergraduate Honors Theses
The method of training for long distance running has been to build up to the actual run distance and to keep similar volume. Although this is great for cardio it neglects the strength aspect of running. Purpose: The purpose of this research was to examine leg strength and compare it to running endurance (VO2) among male recreational runners, in order to determine any relationship. Methods: The subjects will be tested primarily on two pieces of equipment. The isometric mid-thigh clean pull apparatus which is used to measure a subject’s leg strength. The VO2 max machine will be used to determine …
Discovery Of Bald Cypress Fossil Leaves At The Gray Fossil Site, Tennessee And Their Ecological Significance., Sara Brandon
Discovery Of Bald Cypress Fossil Leaves At The Gray Fossil Site, Tennessee And Their Ecological Significance., Sara Brandon
Undergraduate Honors Theses
This study focuses on fossil Taxodium leaves found at the Gray Fossil Site in northeastern Tennessee where many 7-4.5 million year old plants and animals have been recovered. Identification of the leaves is based on comparison of leaf morphology and confirmed by leaf anatomy. The ecological implications of the fossil are discussed to understand the paleoecology and paleoclimate at the Gray site. It is concluded that the fossil plant along with many other plants lived by a large sinkhole lake under a little warmer-than-today’s climate condition.
The Relationship Of Anthropometric Measures To Vertical Jump Height., R. C. Kerns
The Relationship Of Anthropometric Measures To Vertical Jump Height., R. C. Kerns
Undergraduate Honors Theses
The capability to perform your best is an important aspect in the sport of soccer. The relationship between anthropometric measures to vertical jump height among NCAA Division I Women’s soccer players is a subject lacking sufficient scientific research. Purpose: To analyze this relationship, body mass and %body fat was correlated with un-weighted countermovement jump height to determine the strength of this relationship in NCAA DI female soccer players. Methods: Data from an ongoing athlete monitoring program from fourteen NCAA D1 female soccer players in the year 2007 was analyzed. Air Displacement Plethysmography (ADP) via a BOD POD (Life Measurement, Inc., …
Infectious Disease: An Agent-Based Simulation., Iqra Ahmad
Infectious Disease: An Agent-Based Simulation., Iqra Ahmad
Undergraduate Honors Theses
One of the primary reasons for studying infectious diseases is to improve control and ultimately eradicate the infection from the population (Keeling 2008). An agent base model was designed to observe the spread of disease and the effect of vaccination using the program known as StarLogo- TNG. Experiments were designed, not having a particular disease in mind, and simulations were run to determine the effects of different variables on the vaccination process of a certain population. Goals included observation of how infections spread and how vaccination plays a role in various situations such as, root of the infection, population size, …
Puccinia Mariae-Wilsoniae And Claytonia Virginica: A Pathogen's Tale, Sarah A. Schlund
Puccinia Mariae-Wilsoniae And Claytonia Virginica: A Pathogen's Tale, Sarah A. Schlund
Undergraduate Honors Theses
Rusts are economically important fungal plant pathogens. For the majority of rust species, complete life history data, including host range, geographic distribution, plant response to the rust, identity of alternate hosts, and mode of sexual reproduction are incomplete. The purpose of this study was to examine the life history of Puccinia mariae-wilsoniae, a rust on Claytonia virginica (spring beauty), and to observe responses in leaf anatomy to its fungal pathogen. Spring beauty is an ephemeral woodland plant that lasts three to four weeks and P. mariae-wilsoniae infects C. virginica almost as soon as the plant emerges from dormancy in …
Recombination And Screening Of Putative Grapefruit Glucosyltransferase 4 Expressed In Pichia Pastoris., Peri Loftis
Recombination And Screening Of Putative Grapefruit Glucosyltransferase 4 Expressed In Pichia Pastoris., Peri Loftis
Undergraduate Honors Theses
Flavonoids are a group of plant secondary metabolites that are vital to the cell systems of plants. The intake of these chemicals is advantageous to animals for their antioxidant properties that affect the function of immune and inflammatory cells. The bitter taste of grapefruit (Citrus paradisi) and other citrus species is caused by the accumulation of glycosylated flavonoids. Glucosyltransferases (GTs) are enzymes that add glucose moieties to a carbon or hydroxyl group of natural products. The function of a putative secondary product GT clone was tested. In previous research, putative GT 4 was cloned into a pCD1 modified …
Competition In A Simple Pond: A 3d Agent Based Model Approach., Emil W.H. Montano
Competition In A Simple Pond: A 3d Agent Based Model Approach., Emil W.H. Montano
Undergraduate Honors Theses
If two species are competing for a limited resource, the species that uses the resource more efficiently will eventually eliminate the other. This is known as the principle of competitive exclusion developed by Georgy Gause. To determine the effect of this competition, a simple three-dimensional model of a pond is created in which two species compete for a single source of energy (algae). The model is based solely on the conservation of the energy that flows through an ecosystem where primary production is the only source of energy. The first scenario tested is of two competing species with identical life …
Putative Glucosyltransferase 11 From Citrus Paradisi: Cloning, Recombinant Expression In Yeast, And Substrate Screening., Bruce E. Williams
Putative Glucosyltransferase 11 From Citrus Paradisi: Cloning, Recombinant Expression In Yeast, And Substrate Screening., Bruce E. Williams
Undergraduate Honors Theses
Plant secondary products, which include the flavonoids, have a variety of roles in plant systems. Their roles include UV protection, antifeedant activity, pollinator attraction, stress response, and many others. Flavonoids also have effects on human physiology. Glucosylation is an important modification of many flavonoids and other plant secondary products. In grapefruit, glucosylation is important in the synthesis of the bitter compound naringin. Glucosyltransferases catalyze glucosylation reactions. Putative plant secondary product glucosyltransferases may be identified by the loosely conserved “PSPG box” amino acid sequence, and eleven have been isolated to date in Citrus paradisi. With current knowledge, however, biochemical characterization …
Seed Germination And Seed Bank Of Scutellaria Species, Jennifer O'Brien
Seed Germination And Seed Bank Of Scutellaria Species, Jennifer O'Brien
Undergraduate Honors Theses
Florida skullcap, Scutellaria floridana Chapm. (Lamiaceae), is a federally threatened species found in the Florida Panhandle. Its habitat is a fire-prone, longleaf pine forest dominated by Aristida stricta Michx. var. beyrichiana (wiregrass). Other Scutellaria species used in the study are found in southeastern U.S., including two within the Florida Panhandle. The objective of this study was to compare several techniques to germinate seeds of Scutellaria incana, Scutellaria lateriflora, Scutellaria ovata ssp. ovata, and Scutellaria floridana, and to access the presence of a seed bank for S. floridana. For the germination study, Scutellaria later …
An Assessment Of Transformation Protocols Of Trametes Versicolor: A Possible Biological Pretreatment For Biomass, Michelle Hartnett
An Assessment Of Transformation Protocols Of Trametes Versicolor: A Possible Biological Pretreatment For Biomass, Michelle Hartnett
Undergraduate Honors Theses
Lignin is an obstacle in large-scale biofuel production. This can be overcome by the use of fungal pretreatment. This experiment assessed two transformation assays, based on the protocols of Laccaria bicolor and Phanerochaete chrysosporium, and applied them to Trametes versicolor. In order to determine a successful transformation, T. versicolor was transformed with green fluorescence protein (GFP) using the two assays, followed by detection of transformed mycelia under UV light. The two protocols were assessed based on timeliness, ease, and effectiveness. Based on the quantity of viable transformants and ease, the membrane assay described for Laccaria is successful when …
Seed Ecology Of Federally Threatened Pinguicula Ionantha (Godfrey's Butterwort), Jenna Annis
Seed Ecology Of Federally Threatened Pinguicula Ionantha (Godfrey's Butterwort), Jenna Annis
Undergraduate Honors Theses
Pinguicula ionantha G. (Lentibulariaceae) is a carnivorous plant occurring in historically fire-dependent bog habitats of longleaf pine savannas. The species is limited to 6 counties within the Florida Panhandle. The species is currently listed as federally threatened due largely to habitat loss. The objective of this research is to provide the appropriate Pinguicula ionantha germination and seed bank information that would aid in the deli sting of the species. Eleven collections of Pinguicula ionantha seeds were made from natural populations. All germination trials took place in a seed germination chamber. Treatments included stratification, liquid smoke solutions, plant growth regulators (PGR), …
Taking The Road Most Travelled: Understanding Patterns Of Snake (Colubridae; Storeria) Movement And Road Mortality In A State Park, Iwo P. Gross
Undergraduate Honors Theses
No abstract provided.
Improved Tolerance To Freeze And Salt Stress In Mitochondria Of Drosophilia Melanogaster Cells Conferred By Lea Protein, Matthew R. Marunde
Improved Tolerance To Freeze And Salt Stress In Mitochondria Of Drosophilia Melanogaster Cells Conferred By Lea Protein, Matthew R. Marunde
Undergraduate Honors Theses
Mechanisms that govern anhydrobiosis involve the accumulation of highly hydrophilic macromolecules, such as late embryogenesis abundant (LEA) proteins. Group 1 LEA proteins comprised of 181 (AfLEA1.1) and 197 (AfLEA1.3) amino acids were cloned from embryos of Artemia franciscana and expressed in Drosophila melanogaster cells (Kc167). Confocal microscopy revealed accumulations of green fluorescence protein (GFP) and AfLEA1.3 constructs in the mitochondria (AfLEA1.3-GFP) and AfLEA1.1-GFP constructs in the cytoplasm. In the presence of mixed substrates, oxygen consumption was similar for permeabilized Kc167 control and Kc167-AfLEA1.3 cells. Acute titrations of NaCl (up to 500 mM) led to successive drops in oxygen flux of …
Locus Coeruleus And Hippocampal Tyrosine Hydroxylase Levels In A Pressure-Overload Model Of Heart Disease, Luke A. Johnson
Locus Coeruleus And Hippocampal Tyrosine Hydroxylase Levels In A Pressure-Overload Model Of Heart Disease, Luke A. Johnson
Undergraduate Honors Theses
Studies have indicated that approximately 30% of people with heart disease experience major depressive disorder (MDD). Despite strong clinical evidence of a link between the two diseases, the neurobiological processes involved in the relationship are poorly understood. A growing number of studies are revealing similar neuroanatomical and neurochemical abnormalities resulting from both depression and heart disease. The locus coeruleus (LC) is a group of neurons in the pons that synthesize and release norepinephrine, and that is known to play a significant role in depression pathobiology. For example, there is evidence that tyrosine hydroxylase (TH) is elevated in the LC in …