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Investigation Of Antimicrobial Resistance Of Esßl, Amp-C, And Carbapenemaseproducing E. Coli Strains In Retail Poultry Meats, Gülçi̇n Güven, Si̇bel Kizil
Investigation Of Antimicrobial Resistance Of Esßl, Amp-C, And Carbapenemaseproducing E. Coli Strains In Retail Poultry Meats, Gülçi̇n Güven, Si̇bel Kizil
Turkish Journal of Veterinary & Animal Sciences
Antimicrobial agents are used for suppressing some bacterial infections during the rearing of poultry. In this study; the antibiotic resistance properties of Extended Spectrum ßeta-lactamase (ESßL), Amp-C, carbapenemase-producing Escherichia coli (E. coli) strains isolated from chicken breast meat were determined by disc diffusion and E-test methods. Additionally, it is aimed to determine antibiotic resistance genes by classical polymerase chain reaction (PCR). In this context, E. coli strains were isolated from 7 of 100 chicken breast meat (fillets) and identified by a rapid identification system (BBL Crystal). Disc diffusion test results of the isolates were evaluated and 100% to tetracycline; ampicillin, …
Impact Of Ph On The Activity Of Co-Used Antimicrobials Against Resistant Escherichia Colistrains Of Animal Origin, Murat Cengi̇z, Gülçe Hepbostanci
Impact Of Ph On The Activity Of Co-Used Antimicrobials Against Resistant Escherichia Colistrains Of Animal Origin, Murat Cengi̇z, Gülçe Hepbostanci
Turkish Journal of Veterinary & Animal Sciences
In this study, combination antimicrobial therapy, due to its higher potential against resistant bacteria, was evaluated for the inhibition of multidrug-resistant E. coli strains. The influence of pH as an environmental variable on the activity of antimicrobial combinations was evaluated by calculating the factional concentration indexes at pH values 5.0, 6.0, 7.3, and 8.0. The highest synergistic activity rates of ceftriaxone + colistin, danofloxacin + colistin, danofloxacin + ceftiofur, and ceftiofur + gentamicin combinations were 50%, 33%, 100%, and 50%, respectively measured at ≥7.3 pH. The lowest synergistic activity rates for all combinations were observed at the acidic pH values …
Phenotypic And Genotypic Investigation Of The Heavy Metal Resistance In Escherichia Coli Isolates Recovered From Cattle Stool Samples, Ni̇sa Si̇pahi̇, Emre Karakaya, Serkan İki̇z
Phenotypic And Genotypic Investigation Of The Heavy Metal Resistance In Escherichia Coli Isolates Recovered From Cattle Stool Samples, Ni̇sa Si̇pahi̇, Emre Karakaya, Serkan İki̇z
Turkish Journal of Veterinary & Animal Sciences
The purpose of this study was to examine resistance against cadmium (Cd$^{+2}$), copper (Cu$^{+2}$), lead (Pb$^{+2}$), mercury (Hg$^{+2}$), and manganese (Mn$^{+2}$) in Escherichia coli isolates recovered from cattle stool using phenotypic (agar dilution) and genotypic [polymerase chain reaction (PCR)] methods. In addition, the isolates were genotyped via enterobacterial repetitive intergenic consensus polymerase chain reaction (ERIC-PCR). Escherichia coli was isolated and identified from the 100 stool samples that were examined in the study. It was determined phenotypically that all isolates were sensitive to mercury; 97% of them showed resistance to cadmium; 69% showed resistance to copper; 24% showed resistance to lead; …
Isolation And Molecular Characterization Of Salmonella Enterica And Escherichia Coli From Poultry Samples, Ayli̇n Cesur, Saci̇de Özlem Ulutaş, Yeşi̇m Soyer
Isolation And Molecular Characterization Of Salmonella Enterica And Escherichia Coli From Poultry Samples, Ayli̇n Cesur, Saci̇de Özlem Ulutaş, Yeşi̇m Soyer
Turkish Journal of Veterinary & Animal Sciences
Innumerable foodborne pathogens including Salmonella and Escherichia coli pose a serious threat to human health and food safety. As perishable foods, poultry products are considered one of the most common sources of foodborne pathogens including Salmonella and E. coli due to transmission of drug resistance, dissemination of organisms, and cross-contamination. In our study, phenotypic and genotypic characterizations of Salmonella enterica and E. coli isolated from packaged raw chicken products were carried out. Samples belonging to different commercial brands were collected in Ankara in 2015. Among 15 out of 19 Salmonella enterica subsp. enterica strains isolated from different and/or same poultry …
High Level Fluoroquinolone Resistance In Escherichia Coli Isolatedfrom Animals In Turkey Is Due To Multiple Mechanisms, Pinar Şahi̇ntürk, Erdem Arslan, Esra Büyükcangaz, Songül Sonal, Ayşi̇n Şen, Fi̇gen Ersoy, Mark A. Webber, Laura Jv. Piddock, Murat Cengi̇z
High Level Fluoroquinolone Resistance In Escherichia Coli Isolatedfrom Animals In Turkey Is Due To Multiple Mechanisms, Pinar Şahi̇ntürk, Erdem Arslan, Esra Büyükcangaz, Songül Sonal, Ayşi̇n Şen, Fi̇gen Ersoy, Mark A. Webber, Laura Jv. Piddock, Murat Cengi̇z
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to determine the molecular mechanisms of fluoroquinolone resistance in E. Coli isolated from cattle, goats, sheep, cats, and dogs in Turkey. Twenty nonreplicate E. Coli isolates (chosen on the basis of RAPD pattern) from food-producing animals were selected for the study. To identify phenotypic differences between isolates, the sum of the MIC values of 14 antimicrobials was calculated; values ranged from 565 to 2520 μg/mL, indicating the diversity in antimicrobial resistance present in the panel of isolates. PCR and qRT-PCR were used to characterize the presence and expression levels of known molecular mechanisms of …
Application Of Mentofin^® In Broilers With Clinical Infectious Bursal Disease To Reduce Escherichia Coli Related Problems After Vaccination Against Newcastle Disease, K. Tayfun Çarli, Kaan Önat, Elçi̇n Günaydin
Application Of Mentofin^® In Broilers With Clinical Infectious Bursal Disease To Reduce Escherichia Coli Related Problems After Vaccination Against Newcastle Disease, K. Tayfun Çarli, Kaan Önat, Elçi̇n Günaydin
Turkish Journal of Veterinary & Animal Sciences
In this study Mentofin^® was used in commercial broiler chickens naturally exposed to infectious bursal disease virus (IBDV) and administered live Newcastle disease virus (NDV) vaccine to evaluate the ability of Mentofin^® to reduce Escherichia coli-related respiratory lesions, immunomodulate Newcastle disease (ND) vaccine response, change pharyngeal aerobic bacterial counts, and have certain impacts on specific production parameters. Mentofin^® was added to the drinking water (100 ml/500 l water) of broiler chickens aged between 3 and 5 days. ND and IBD vaccinations were administered via drinking water to 15-day-old chickens. NDV vaccination was given again when the chickens were 20 days …
Plasmids And Protein Patterns Of Escherichia Coli Isolated From Bovine Mastitis In Konya, Turkey, Uçkun Sai̇t Uçan, Leyla Açik, Ayten Çelebi̇, Osman Ergani̇ş, Emi̇ne Arslan
Plasmids And Protein Patterns Of Escherichia Coli Isolated From Bovine Mastitis In Konya, Turkey, Uçkun Sai̇t Uçan, Leyla Açik, Ayten Çelebi̇, Osman Ergani̇ş, Emi̇ne Arslan
Turkish Journal of Veterinary & Animal Sciences
In this study, a total of 30 Escherichia coli isolates obtained from milk samples of dairy cows suffering from subclinical mastitis in Konya, Turkey were typed according to plasmids and protein patterns. Agarose gel electrophoresis and sodium doedecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) methods were used to identify plasmids and whole-cell protein profiles. Of these two methods, SDS-PAGE typing proved to be more discriminate for typing the isolates.
The Need For Confirmation In Coliform And E. Coli Enumeration In Foods, İbrahi̇m Çakir, Hi̇lal B. Doğan, Ensar Başpinar, Fi̇kret Keven, A. Kadi̇r Halkman
The Need For Confirmation In Coliform And E. Coli Enumeration In Foods, İbrahi̇m Çakir, Hi̇lal B. Doğan, Ensar Başpinar, Fi̇kret Keven, A. Kadi̇r Halkman
Turkish Journal of Veterinary & Animal Sciences
For food and environmental analysis, coliform bacteria and especially E. coli should be determined and enumerated rapidly, correctly and economically. Even if the results are obtained either by traditional or improved techniques, the results require confirmation, meaning that these techniques are not reliable. Confirmation tests need additional time and/or cost. In this study, 500 food samples of 10 various types were analyzed for their natural coliform contamination by the standard MPN method and E. coli by two MUG based MPN techniques. Enumeration results were statistically analyzed to determine whether confirmation tests for coliform and E. coli analysis are necessary or …
Determination Of Microbiological Quality Of Ice-Creambelong To Various Patisserie In Ankara, İrfan Erol, Özlem Küplülü, Belgin Siriken, T. Halûk Çeli̇k
Determination Of Microbiological Quality Of Ice-Creambelong To Various Patisserie In Ankara, İrfan Erol, Özlem Küplülü, Belgin Siriken, T. Halûk Çeli̇k
Turkish Journal of Veterinary & Animal Sciences
In this study, a total of 100 ice cream samples, collected from various patisserie in Ankara, 30 of them vanilla, 26 of them chocolate and 44 of them fruit ice cream samples, were analysed microbiologically. While coliform and E. coli counts were determined with MPN technique, in the isolation of the salmonella enrichment technique and in the isolation of the total viable count, lactobacilli, micrococci and staphylococci, coagulase positive staphylococci, enterobacteriaceae, enterococci, pseudomonas, yeast and molds, drop plating technique were used. According to analysis findings, it was determined that 63.2% to 73.1% of vanilla ice cream samples, 73.0% to 57.4% …