Life Sciences Commons^™

Loss Of Glycosaminoglycan Receptor Binding After Mosquito Cell Passage Reduces Chikungunya Virus Infectivity, Amber M. Paul, Dhiraj Acharya, John F. Anderson, Faqing Huang, Fengwei Bai

Publications

Chikungunya virus (CHIKV) is a mosquito-transmitted alphavirus that can cause fever and chronic arthritis in humans. CHIKV that is generated in mosquito or mammalian cells differs in glycosylation patterns of viral proteins, which may affect its replication and virulence. Herein, we compare replication, pathogenicity, and receptor binding of CHIKV generated in Vero cells (mammal) or C6/36 cells (mosquito) through a single passage. We demonstrate that mosquito cell derived CHIKV (CHIKVmos) has slower replication than mammalian cell derived CHIKV (CHIKVvero), when tested in both human and murine cell lines. Consistent with this, CHIKVmos infection in both cell lines produce less cytopathic …

Enhancing Genome Investigations In The Mosquito Culex Quinquefasciatus Via Bac Library Construction And Characterization, Paul V. Hickner, Becky Debruyn, Diane D. Lovin, Akio Mori, Christopher Saski, David W. Severson

Publications

Background

Culex quinquefasciatus (Say) is a major species in the Culex pipiens complex and an important vector for several human pathogens including West Nile virus and parasitic filarial nematodes causing lymphatic filariasis. It is common throughout tropical and subtropical regions and is among the most geographically widespread mosquito species. Although the complete genome sequence is now available, additional genomic tools are needed to improve the sequence assembly.

Findings

We constructed a bacterial artificial chromosome (BAC) library using the pIndigoBAC536 vector and HindIII partially digested DNA isolated from Cx. quinquefasciatus pupae, Johannesburg strain (NDJ). Insert size was estimated by Not …

Aquatic Insects Of Upper Three Runs Creek, Savannah River Plant, South Carolina. Part Ii: Diptera, John C. Morse, Jay W. Chapin, David D. Herlong, Ray S. Harvey

Publications

As discussed in detail in Part I of this series (Morse et al. 1980), aquatic insects were collected biweekly from six locations on Upper Three Runs Creek, Savannah River Plant near Aiken, South Carolina, from September 1976 through August 1977, using light traps and semi-quantitative benthic sampling methods. See part I (Morse et al. 1980) for site and habitat descriptions and for a discussion of the methods used. In that publication, faunistic results were provided for all insect taxa except Diptera and relative abundance data for all but Diptera and non-dryopoid Coleoptera.