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Michigan Tech Publications

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Full-Text Articles in Life Sciences

A High-Efficiency Gene Silencing In Plants Using Two-Hit Asymmetrical Artificial Micrornas, Sachin Teotia, Xiaoran Wang, Na Zhou, Mengmeng Wang, Haiping Liu, Jun Qin, Dianwei Han, Chingwen Li, Christine E. Li, Shangjin Pan, Haifeng Tang, Wenjun Kang, Zhanhui Zhang, Xiaoqing Tang, Ting Peng, Guiliang Tang Sep 2023

A High-Efficiency Gene Silencing In Plants Using Two-Hit Asymmetrical Artificial Micrornas, Sachin Teotia, Xiaoran Wang, Na Zhou, Mengmeng Wang, Haiping Liu, Jun Qin, Dianwei Han, Chingwen Li, Christine E. Li, Shangjin Pan, Haifeng Tang, Wenjun Kang, Zhanhui Zhang, Xiaoqing Tang, Ting Peng, Guiliang Tang

Michigan Tech Publications

MicroRNAs (miRNAs) are small non-coding RNA molecules that play a crucial role in gene regulation. They are produced through an enzyme-guided process called dicing and have an asymmetrical structure with two nucleotide overhangs at the 3' ends. Artificial microRNAs (amiRNAs or amiRs) are designed to mimic the structure of miRNAs and can be used to silence specific genes of interest. Traditionally, amiRNAs are designed based on an endogenous miRNA precursor with certain mismatches at specific positions to increase their efficiency. In this study, the authors modified the highly expressed miR168a in Arabidopsis thaliana by replacing the single miR168 stem-loop / …


Impacts Of Microrna-483 On Human Diseases, Katy Matson, Aaron B. Macleod, Nirali Mehta, Ellie Sempek, Xiaoqing Tang Jun 2023

Impacts Of Microrna-483 On Human Diseases, Katy Matson, Aaron B. Macleod, Nirali Mehta, Ellie Sempek, Xiaoqing Tang

Michigan Tech Publications

MicroRNAs (miRNAs) are short non-coding RNA molecules that regulate gene expression by targeting specific messenger RNAs (mRNAs) in distinct cell types. This review provides a com-prehensive overview of the current understanding regarding the involvement of miR-483-5p and miR-483-3p in various physiological and pathological processes. Downregulation of miR-483-5p has been linked to numerous diseases, including type 2 diabetes, fatty liver disease, diabetic nephropathy, and neurological injury. Accumulating evidence indicates that miR-483-5p plays a crucial protective role in preserving cell function and viability by targeting specific transcripts. Notably, elevated levels of miR-483-5p in the bloodstream strongly correlate with metabolic risk factors and …


Using Culturally Significant Birds To Guide The Timing Of Prescribed Fires In The Klamath Siskiyou Bioregion, Linda L. Long, Frank L. Lake, Jaime L. Stephens, John D. Alexander, C. John Ralph, Jared D. Wolfe Jun 2023

Using Culturally Significant Birds To Guide The Timing Of Prescribed Fires In The Klamath Siskiyou Bioregion, Linda L. Long, Frank L. Lake, Jaime L. Stephens, John D. Alexander, C. John Ralph, Jared D. Wolfe

Michigan Tech Publications

Historically, wildfire and tribal burning practices played important roles in shaping ecosystems throughout the Klamath Siskiyou Bioregion of northern California and southern Oregon. Over the past several decades, there has been increased interest in the application of fire for forest management through the implementation of prescribed fires within habitats that are used by a diversity of migrant and resident land birds. While many bird species may benefit from habitat enhancements associated with wildfires, cultural burning, and prescribed fire, individuals may face direct or indirect harm. In this study, we analyzed the timing of breeding and molting in 11 species of …


Agroforestry Buffers On Nitrogen Reduction In Groundwater On A Grazed Hillslope, Miguel Salceda, Ranjith P. Udawatta, Stephen H. Anderson, Sidath S. Mendis, Fengjing Liu Jun 2023

Agroforestry Buffers On Nitrogen Reduction In Groundwater On A Grazed Hillslope, Miguel Salceda, Ranjith P. Udawatta, Stephen H. Anderson, Sidath S. Mendis, Fengjing Liu

Michigan Tech Publications

Agricultural practices often contribute to the transport of solutes into groundwater; thus, low-cost strategies that extract nutrients from groundwater are essential to address water pollution. This study evaluated the effects of agroforestry (tree + grass; AB [cottonwood {Populus deltoides Bortr. ex Marsh.}]) and grass buffers (GB; [tall fescue Schedonorus phoenix (Scop.) Holub, Red clover {Trifolium pretense L.}, and Lespedeza {Lespedeza Michx}]) on groundwater nitrogen (N) concentrations. The experiment consisted of two grazing watersheds, one with an AB and another with a GB treatment. Buffers were not grazed since 2001. Three wells representing summit, backslope, and foot-slope positions were installed at …


Deconstructed Plastic Substrate Preferences Of Microbial Populations From The Natural Environment, Lindsay I. Putman, Laura G. Schaerer, Ruochen Wu, Daniel G. Kulas, Ali Zolghadr, Rebecca Ong, David Shonnard, Stephen Techtmann Jun 2023

Deconstructed Plastic Substrate Preferences Of Microbial Populations From The Natural Environment, Lindsay I. Putman, Laura G. Schaerer, Ruochen Wu, Daniel G. Kulas, Ali Zolghadr, Rebecca Ong, David Shonnard, Stephen Techtmann

Michigan Tech Publications

Over half of the world's plastic waste is landfilled, where it is estimated to take hundreds of years to degrade. Given the continued use and disposal of plastic products, it is vital that we develop fast and effective ways to utilize plastic waste. Here, we explore the potential of tandem chemical and biological processing to process various plastics quickly and effectively. Four samples of compost or sediment were used to set up enrichment cultures grown on mixtures of compounds, including disodium terephthalate and terephthalic acid (monomers of polyethylene terephthalate), compounds derived from the chemical deconstruction of polycarbonate, and pyrolysis oil …


Editorial: Gulf Of Mexico Estuaries: Ecology Of The Nearshore And Coastal Ecosystems Impacted By The Deepwater Horizon Oil Spill, Charles W. Martin, Paola C. López-Duarte, Jill A. Olin, Brian J. Roberts May 2023

Editorial: Gulf Of Mexico Estuaries: Ecology Of The Nearshore And Coastal Ecosystems Impacted By The Deepwater Horizon Oil Spill, Charles W. Martin, Paola C. López-Duarte, Jill A. Olin, Brian J. Roberts

Michigan Tech Publications

No abstract provided.


Chemically Catalyzed Phytoextraction For Sustainable Cleanup Of Soil Lead Contamination In A Community Garden In Jersey City, New Jersey, Zhiming Zhang, Dibyendu Sarkar, Frances Levy, Rupali Datta May 2023

Chemically Catalyzed Phytoextraction For Sustainable Cleanup Of Soil Lead Contamination In A Community Garden In Jersey City, New Jersey, Zhiming Zhang, Dibyendu Sarkar, Frances Levy, Rupali Datta

Michigan Tech Publications

Soil lead (Pb) contamination in Pb paint-contaminated homes is a serious health risk in urban areas. Phytoextraction is a green and sustainable technology for soil Pb remediation, but its efficiency depends on the geochemical partitioning of Pb in soil. Following successful laboratory, greenhouse, and panel experiments, a field study was conducted to demonstrate the effectiveness of a chemically catalyzed phytoextraction model for Pb removal. A biodegradable chelating agent, ethylenediaminedisuccinic acid (EDDS) was applied during Pb phytoextraction by vetiver grass (Chrysopogon zizanioides) in a Pb-contaminated community garden in Jersey City, New Jersey. Results showed that soil Pb concentration was reduced from …


A Review Of Propagation And Restoration Techniques For American Beech And Their Current And Future Application In Mitigation Of Beech Bark Disease, Andrea L. Myers, Andrew J. Storer, Yvette L. Dickinson, Tara Bal May 2023

A Review Of Propagation And Restoration Techniques For American Beech And Their Current And Future Application In Mitigation Of Beech Bark Disease, Andrea L. Myers, Andrew J. Storer, Yvette L. Dickinson, Tara Bal

Michigan Tech Publications

The American beech (Fagus grandifolia Ehrh.) has been impacted by the beech bark disease (BBD) complex throughout the northeastern United States for over 100 years, but the disease has been present in the Great Lakes region only for around 20 years, requiring acknowledgement of the evolving context surrounding F. grandifolia. This disease threatens to remove a foundational tree species which is especially important ecologically for wildlife habitat and mast, and as a climax successional species. We review advances in propagation techniques of F. grandifolia with the goal of addressing their use in the rehabilitative restoration of forests affected by BBD. …


Mechanical Properties And Morphological Alterations In Fiber-Based Scaffolds Affecting Tissue Engineering Outcomes, James Dolgin, Samerender Nagam Hanumantharao, Stephen Farias, Carl G. Simon, Smitha Rao Apr 2023

Mechanical Properties And Morphological Alterations In Fiber-Based Scaffolds Affecting Tissue Engineering Outcomes, James Dolgin, Samerender Nagam Hanumantharao, Stephen Farias, Carl G. Simon, Smitha Rao

Michigan Tech Publications

Electrospinning is a versatile tool used to produce highly customizable nonwoven nanofiber mats of various fiber diameters, pore sizes, and alignment. It is possible to create electrospun mats from synthetic polymers, biobased polymers, and combinations thereof. The post-processing of the end products can occur in many ways, such as cross-linking, enzyme linking, and thermal curing, to achieve enhanced chemical and physical properties. Such multi-factor tunability is very promising in applications such as tissue engineering, 3D organs/organoids, and cell differentiation. While the established methods involve the use of soluble small molecules, growth factors, stereolithography, and micro-patterning, electrospinning involves an inexpensive, labor …


Mountain Wetland Soil Carbon Stocks Of Huascarán National Park, Peru, Rodney Chimner, Sigrid Resh, John A. Hribljan, Michael Battaglia, Laura Bourgeau-Chavez, Gillian Bowser, Erik A. Lilleskov Apr 2023

Mountain Wetland Soil Carbon Stocks Of Huascarán National Park, Peru, Rodney Chimner, Sigrid Resh, John A. Hribljan, Michael Battaglia, Laura Bourgeau-Chavez, Gillian Bowser, Erik A. Lilleskov

Michigan Tech Publications

Although wetlands contain a disproportionately high amount of earth’s total soil carbon, many regions are still poorly mapped and with unquantified carbon stocks. The tropical Andes contain a high concentration of wetlands consisting mostly of wet meadows and peatlands, yet their total organic carbon stocks are poorly quantified, as well as the carbon fraction that wet meadows store compared to peatlands. Therefore, our goal was to quantify how soil carbon stocks vary between wet meadows and peatlands for a previously mapped Andean region, Huascarán National Park, Peru. Our secondary goal was to test a rapid peat sampling protocol to facilitate …


The Methylation Landscape Of Giga-Genome And The Epigenetic Timer Of Age In Chinese Pine, Jiang Li, Fangxu Han, Tongqi Yuan, Wei Li, Yue Li, Harry X. Wu, Hairong Wei, Shihui Niu Apr 2023

The Methylation Landscape Of Giga-Genome And The Epigenetic Timer Of Age In Chinese Pine, Jiang Li, Fangxu Han, Tongqi Yuan, Wei Li, Yue Li, Harry X. Wu, Hairong Wei, Shihui Niu

Michigan Tech Publications

Epigenetics has been revealed to play a crucial role in the long-term memory in plants. However, little is known about whether the epigenetic modifications occur with age progressively in conifers. Here, we present the single-base resolution DNA methylation landscapes of the 25-gigabase Chinese pine (Pinus tabuliformis) genome at different ages. The result shows that DNA methylation is closely coupled with the regulation of gene transcription. The age-dependent methylation profile with a linearly increasing trend is the most significant pattern of DMRs between ages. Two segments at the five-prime end of the first ultra-long intron in DAL1, a conservative age biomarker …


Forming Regional Soil Carbon Networks To Support Effective Climate Change Solutions, Gregory Lawrence, Ivan Fernandez, Scott Bailey, Colin Beier, Alexandra Contosta, Erin Lane, Peter Murdoch, Lucas E. Nave, Angelica Quintana, Donald Ross, Alissa White Apr 2023

Forming Regional Soil Carbon Networks To Support Effective Climate Change Solutions, Gregory Lawrence, Ivan Fernandez, Scott Bailey, Colin Beier, Alexandra Contosta, Erin Lane, Peter Murdoch, Lucas E. Nave, Angelica Quintana, Donald Ross, Alissa White

Michigan Tech Publications

Sequestration and storage of organic carbon (C) in soil is an essential component of climate change mitigation and fundamental in promoting the health and climate resilience of soils. Sources of available soil C data are increasing, which complicates efforts to consolidate the data in forms that can be readily used by stakeholders. Spatial and temporal gaps in data availability also limit the quantification of changes in soil C through space and time. Improved coordination among producers and users of soil C data would provide data compatibility at the spatial and temporal resolution required for C monitoring, accounting, and verification of …


Canopy-Top Measurements Do Not Accurately Quantify Canopy-Scale Leaf Thermoregulation, Josef C. Garen, Luiza Maria T. Aparecido, Benjamin W. Blonder, Molly A. Cavaleri, Martijn Slot, Sean T. Michaletz Apr 2023

Canopy-Top Measurements Do Not Accurately Quantify Canopy-Scale Leaf Thermoregulation, Josef C. Garen, Luiza Maria T. Aparecido, Benjamin W. Blonder, Molly A. Cavaleri, Martijn Slot, Sean T. Michaletz

Michigan Tech Publications

No abstract provided.


Coarse Woody Debris And Carbon Stocks In Pine Forests After 50 Years Of Recovery From Harvesting In Northeastern California, Jianwei Zhang, Deborah S. Page-Dumroese, Martin Jurgensen, Matt Busse, Kim G. Mattson Mar 2023

Coarse Woody Debris And Carbon Stocks In Pine Forests After 50 Years Of Recovery From Harvesting In Northeastern California, Jianwei Zhang, Deborah S. Page-Dumroese, Martin Jurgensen, Matt Busse, Kim G. Mattson

Michigan Tech Publications

The long-term effects of harvesting on stand carbon (C) pools were assessed in a dry, interior pine-dominated forest at the Blacks Mountain Experimental Forest in northeastern California. Six 8-hectacre plots, established in 1938–1943, were treated as either an uncut control or a heavy-cut harvest (three-quarters of the stand volume removed). Response variables included C pools in overstory tree and shrub, coarse woody debris (CWD), forest floor, mineral soil (to 30 cm depth), cubicle brown root fragments of wood, fine roots, and ectomycorrhizal root tips. CWD was further classified as intact wood or more highly decayed brown rot or white rot …


Autotetraploidization Alters Morphology, Photosynthesis, Cytological Characteristics And Fruit Quality In Sour Jujube (Ziziphus Acidojujuba Cheng Et Liu), Lihu Wang, Lixin Wang, Tingting Ye, Jin Zhao, Lili Wang, Hairong Wei, Ping Liu, Mengjun Liu Mar 2023

Autotetraploidization Alters Morphology, Photosynthesis, Cytological Characteristics And Fruit Quality In Sour Jujube (Ziziphus Acidojujuba Cheng Et Liu), Lihu Wang, Lixin Wang, Tingting Ye, Jin Zhao, Lili Wang, Hairong Wei, Ping Liu, Mengjun Liu

Michigan Tech Publications

Artificially induced polyploidization is one of the most effective techniques for improving the biological properties and creating new cultivars of fruit trees. Up to now, systematic research on the autotetraploid of sour jujube (Ziziphus acidojujuba Cheng et Liu) has not been reported. ‘Zhuguang’ is the first released autotetraploid sour jujube induced with colchicine. The objective of this study was to compare the differences in the morphological, cytological characteristics, and fruit quality between diploid and autotetraploid. Compared with the original diploid, ‘Zhuguang’ showed dwarf phenotypes and decreased tree vigor. The sizes of the flowers, pollen, stomata, and leaves of ‘Zhuguang’ were …


Gloria - A Globally Representative Hyperspectral In Situ Dataset For Optical Sensing Of Water Quality, Moritz K. Lehmann, Daniela Gurlin, Nima Pahlevan, Krista Alikas, Janet Anstee, Sundarabalan V. Balasubramanian, Cláudio C.F. Barbosa, Caren Binding, Astrid Bracher, Mariano Bresciani, Ashley Burtner, Zhigang Cao, Arnold G. Dekker, Courtney Di Vittorio, Nathan Drayson, Reagan M. Errera, Virginia Fernandez, Dariusz Ficek, Cédric G. Fichot, Peter Gege, Claudia Giardino, Anatoly A. Gitelson, Steven R. Greb, Hayden Henderson, Hiroto Higa, Abolfazl Irani Rahaghi, Cédric Jamet, Dalin Jiang, Thomas Jordan, Kersti Kangro, Jeremy A. Kravitz Feb 2023

Gloria - A Globally Representative Hyperspectral In Situ Dataset For Optical Sensing Of Water Quality, Moritz K. Lehmann, Daniela Gurlin, Nima Pahlevan, Krista Alikas, Janet Anstee, Sundarabalan V. Balasubramanian, Cláudio C.F. Barbosa, Caren Binding, Astrid Bracher, Mariano Bresciani, Ashley Burtner, Zhigang Cao, Arnold G. Dekker, Courtney Di Vittorio, Nathan Drayson, Reagan M. Errera, Virginia Fernandez, Dariusz Ficek, Cédric G. Fichot, Peter Gege, Claudia Giardino, Anatoly A. Gitelson, Steven R. Greb, Hayden Henderson, Hiroto Higa, Abolfazl Irani Rahaghi, Cédric Jamet, Dalin Jiang, Thomas Jordan, Kersti Kangro, Jeremy A. Kravitz

Michigan Tech Publications

The development of algorithms for remote sensing of water quality (RSWQ) requires a large amount of in situ data to account for the bio-geo-optical diversity of inland and coastal waters. The GLObal Reflectance community dataset for Imaging and optical sensing of Aquatic environments (GLORIA) includes 7,572 curated hyperspectral remote sensing reflectance measurements at 1 nm intervals within the 350 to 900 nm wavelength range. In addition, at least one co-located water quality measurement of chlorophyll a, total suspended solids, absorption by dissolved substances, and Secchi depth, is provided. The data were contributed by researchers affiliated with 59 institutions worldwide and …


Correction To: Determining Puma Habitat Suitability In The Eastern Usa (Biodiversity And Conservation, (2023), 10.1007/S10531-022-02529-Z), Veronica Yovovich, Nathaniel Robinson, Hugh Robinson, Michael J. Manfredo, Shelby Perry, Jeremy T. Bruskotter, John A. Vucetich, Luis Aníbal Solórzano, Lydia A. Roe, Alison Lesure, Jamie Robertson, Tom Butler, L. Mark Elbroch Feb 2023

Correction To: Determining Puma Habitat Suitability In The Eastern Usa (Biodiversity And Conservation, (2023), 10.1007/S10531-022-02529-Z), Veronica Yovovich, Nathaniel Robinson, Hugh Robinson, Michael J. Manfredo, Shelby Perry, Jeremy T. Bruskotter, John A. Vucetich, Luis Aníbal Solórzano, Lydia A. Roe, Alison Lesure, Jamie Robertson, Tom Butler, L. Mark Elbroch

Michigan Tech Publications

In the original article, One of the author names was incorrectly misspelled as Tom Bulter. It must be published as “Tom Butler”. The original article has been corrected.


Genomic Underpinnings Of Population Persistence In Isle Royale Moose, Christopher C. Kyriazis, Annabel C. Beichman, Kristin Brzeski, Sarah Hoy, Rolf O. Peterson, John A. Vucetich, Leah Vucetich, Kirk E. Lohmueller, Robert K. Wayne Feb 2023

Genomic Underpinnings Of Population Persistence In Isle Royale Moose, Christopher C. Kyriazis, Annabel C. Beichman, Kristin Brzeski, Sarah Hoy, Rolf O. Peterson, John A. Vucetich, Leah Vucetich, Kirk E. Lohmueller, Robert K. Wayne

Michigan Tech Publications

Island ecosystems provide natural laboratories to assess the impacts of isolation on population persistence. However, most studies of persistence have focused on a single species, without comparisons to other organisms they interact with in the ecosystem. The case study of moose and gray wolves on Isle Royale allows for a direct contrast of genetic variation in isolated populations that have experienced dramatically differing population trajectories over the past decade. Whereas the Isle Royale wolf population recently declined nearly to extinction due to severe inbreeding depression, the moose population has thrived and continues to persist, despite having low genetic diversity and …


Forest Ecosystem Properties Emerge From Interactions Of Structure And Disturbance, J. Christina Mitchell, Daniel M. Kashian, Xiongwen Chen, Stella Cousins, David J. Flaspohler, Daniel S. Gruner, Jeremy S. Johnson, Thilina D. Surasinghe, Jenny Zambrano, Brian Buma Feb 2023

Forest Ecosystem Properties Emerge From Interactions Of Structure And Disturbance, J. Christina Mitchell, Daniel M. Kashian, Xiongwen Chen, Stella Cousins, David J. Flaspohler, Daniel S. Gruner, Jeremy S. Johnson, Thilina D. Surasinghe, Jenny Zambrano, Brian Buma

Michigan Tech Publications

Forest structural diversity and its spatiotemporal variability are constrained by environmental and biological factors, including species pools, climate, land-use history, and legacies of disturbance regimes. These factors influence forest responses to disturbances and their interactions with structural diversity, potentially creating structurally mediated emergent properties at local to continental spatial scales and over evolutionary time. Here, we present a conceptual framework for exploring the emergent properties that arise from interactions between forest structural diversity and disturbances. We synthesize and present definitions for key terms, including emergent property, disturbance, and resilience, and highlight various types and examples of emergent properties, such as …


Properties And Emissions Of Three-Layer Particleboards Manufactured With Mixtures Of Wood Chips And Partially Liquefied Bark, Wen Jiang, Stergios Adamopoulos, Reza Hosseinpourpia, Thomas Walther, Sergej Medved Feb 2023

Properties And Emissions Of Three-Layer Particleboards Manufactured With Mixtures Of Wood Chips And Partially Liquefied Bark, Wen Jiang, Stergios Adamopoulos, Reza Hosseinpourpia, Thomas Walther, Sergej Medved

Michigan Tech Publications

Partial liquefaction of residual biomass shows good potential for developing new materials suitable for making bio-based composites. Three-layer particleboards were produced by replacing virgin wood particles with partially liquefied bark (PLB) in the core or surface layers. PLB was prepared by the acid-catalyzed liquefaction of industrial bark residues in polyhydric alcohol. The chemical and microscopic structure of bark and residues after liquefaction were evaluated by means of Fourier Transform Infrared Spectroscopy (FTIR) and Scanning Electron Microscopy (SEM), while the particleboards were tested for their mechanical and water-related properties, as well as their emission profiles. Through a partial liquefaction process, some …


Tree Stem Volume Estimation From Terrestrial Lidar Point Cloud By Unwrapping, Zhongming An, Robert E. Froese Feb 2023

Tree Stem Volume Estimation From Terrestrial Lidar Point Cloud By Unwrapping, Zhongming An, Robert E. Froese

Michigan Tech Publications

Estimating the volume of standing trees is a fundamental concern in forestry and is typically accomplished using one or more measurements of stem diameter along with formulae that assume geometric primitives. In contrast, technologies such as terrestrial Light Detection And Ranging (LiDAR) can record very detailed spatial information on the actual surface of an object, such as a tree bole.We present a method using LiDAR that provides accurate volume estimates of tree stems, as well as 2D rasters that display details of stem surfaces, which we call the “unwrapping method.” This method combines the concepts of cylinder fitting, voxelization, and …


Comparison Of High-Resolution Naip And Unmanned Aerial Vehicle (Uav) Imagery For Natural Vegetation Communities Classification Using Machine Learning Approaches, Parth Bhatt, Ann Maclean Feb 2023

Comparison Of High-Resolution Naip And Unmanned Aerial Vehicle (Uav) Imagery For Natural Vegetation Communities Classification Using Machine Learning Approaches, Parth Bhatt, Ann Maclean

Michigan Tech Publications

To map and manage forest vegetation including wetland communities, remote sensing technology has been shown to be a valid and widely employed technology. In this paper, two ecologically different study areas were evaluated using free and widely available high-resolution multispectral National Agriculture Imagery Program (NAIP) and ultra-high-resolution multispectral unmanned aerial vehicle (UAV) imagery located in the Upper Great Lakes Laurentian Mixed Forest. Three different machine learning algorithms, random forest (RF), support vector machine (SVM), and averaged neural network (avNNet), were evaluated to classify complex natural habitat communities as defined by the Michigan Natural Features Inventory. Accurate training sets were developed …


Structuring Life After Death: Plant Leachates Promote Co2 Uptake By Regulating Microbial Biofilm Interactions In A Northern Peatland Ecosystem, Allison R. Rober, Allyson J. Lankford, Evan Kane, Merritt R. Turetsky, Kevin H. Wyatt Jan 2023

Structuring Life After Death: Plant Leachates Promote Co2 Uptake By Regulating Microbial Biofilm Interactions In A Northern Peatland Ecosystem, Allison R. Rober, Allyson J. Lankford, Evan Kane, Merritt R. Turetsky, Kevin H. Wyatt

Michigan Tech Publications

Shifts in plant functional groups associated with climate change have the potential to influence peatland carbon storage by altering the amount and composition of organic matter available to aquatic microbial biofilms. The goal of this study was to evaluate the potential for plant subsidies to regulate ecosystem carbon flux (CO2) by governing the relative proportion of primary producers (microalgae) and heterotrophic decomposers (heterotrophic bacteria) during aquatic biofilm development in an Alaskan fen. We evaluated biofilm composition and CO2 flux inside mesocosms with and without nutrients (both nitrogen and phosphorus), organic carbon (glucose), and leachates from common peatland plants (moss, sedge, …


The World Of Underground Ecology In A Changing Environment, Elsa Abs, Moira Hough Jan 2023

The World Of Underground Ecology In A Changing Environment, Elsa Abs, Moira Hough

Michigan Tech Publications

This special feature presents state-of-the-art soil ecological science and was sparked following the 2-day long online live event entitled “Ecology Underground” during the Ecological Society of America annual meeting of 2020. Here, we, the co-guest-editors of this special feature, present this body of research in context of the current state of the field. This issue highlights that we are currently in a hot time for microbial research in soil science. Specifically, we find that two themes emerge from this corpus as key next questions to answer to move the field forward. How do microbial processes scale up in space and …


Degradation Assessment Of Archaeological Oak (Quercus Spp.) Buried Under Oxygen-Limited Condition, Amir Ghavidel, Amin Jorbandian, Miklos Bak, Jana Gelbrich, Jeffrey J. Morrell, Ion Sandu, Reza Hosseinpourpia Jan 2023

Degradation Assessment Of Archaeological Oak (Quercus Spp.) Buried Under Oxygen-Limited Condition, Amir Ghavidel, Amin Jorbandian, Miklos Bak, Jana Gelbrich, Jeffrey J. Morrell, Ion Sandu, Reza Hosseinpourpia

Michigan Tech Publications

The biological deterioration of archaeological wood under oxygen-limited conditions varies due to the limited activities of microorganisms. It is essential to expand the knowledge of the degradation types and the status of archaeological monuments for selecting the proper consolidates. The physical, chemical, and anatomical properties of approximately 600-650 year old archaeological oak collected from an archaeological site in Iasi-Romania were analysed to assess the quality and to identify the degradation types. The results were compared with similar tests on recently-cut oak. X-ray photoelectron spectroscopy (XPS) revealed the presence of more lignin-related peaks in the archaeological oak, which likely reflected the …


Determining Puma Habitat Suitability In The Eastern Usa, Veronica Yovovich, Nathaniel Robinson, Hugh Robinson, Michael J. Manfredo, Shelby Perry, Jeremy T. Bruskotter, John Vucetich, Luis Aníbal Solórzano, Lydia A. Roe, Alison Lesure, Jamie Robertson, Tom Bulter, L. Mark Elbroch Jan 2023

Determining Puma Habitat Suitability In The Eastern Usa, Veronica Yovovich, Nathaniel Robinson, Hugh Robinson, Michael J. Manfredo, Shelby Perry, Jeremy T. Bruskotter, John Vucetich, Luis Aníbal Solórzano, Lydia A. Roe, Alison Lesure, Jamie Robertson, Tom Bulter, L. Mark Elbroch

Michigan Tech Publications

Pumas (Puma concolor) were eliminated from most of the eastern USA a century ago. In the past couple of decades, their recovery in the West has increased puma dispersal into the Midwest, with some individuals even traveling to the East Coast. We combined published expert opinion data and a habitat suitability index in an analysis that identified 17 areas in the Upper Midwest, Ozarks, Appalachia, and New England that could potentially host puma populations in the future. Thirteen of these were larger than 10,000 km2 and so likely to ensure a puma population’s long-term genetic health. Further, we quantified patch …


Restoring Human And More-Than-Human Relations In Toxic Riskscapes: “In Perpetuity” Within Lake Superior’S Keweenaw Bay Indian Community, Sand Point, Valoree Gagnon, Evelyn H. Ravindran Jan 2023

Restoring Human And More-Than-Human Relations In Toxic Riskscapes: “In Perpetuity” Within Lake Superior’S Keweenaw Bay Indian Community, Sand Point, Valoree Gagnon, Evelyn H. Ravindran

Michigan Tech Publications

Lake Superior’s Keweenaw Bay is the ancestral and contemporary homeland of the Anishinaabe Ojibwa and their relatives. It is also a toxic riskscape: Its waters, shorelines, and fish beings are polluted by an unknown tonnage of legacy mining waste rock called “stamp sands,” which contain unsafe levels of toxic compounds. This paper describes Ojibwa stewardship principles and reciprocal obligations, illustrating First Treaty With Gichi-Manitou practices of restoring relations within a toxic riskscape. Defined here, riskscapes are places and spaces where pollution/toxicity relations are continually reconfigured in literal, symbolic, and systemic ways. We share a story from Keweenaw Bay’s Sand Point …


Genome-Wide Identification And Characterization Of Auxin Response Factor (Arf) Gene Family Involved In Wood Formation And Response To Exogenous Hormone Treatment In Populus Trichocarpa, Yingying Liu, Ruiqi Wang, Jiajie Yu, Shan Huang, Yang Zhang, Hairong Wei, Zhigang Wei Jan 2023

Genome-Wide Identification And Characterization Of Auxin Response Factor (Arf) Gene Family Involved In Wood Formation And Response To Exogenous Hormone Treatment In Populus Trichocarpa, Yingying Liu, Ruiqi Wang, Jiajie Yu, Shan Huang, Yang Zhang, Hairong Wei, Zhigang Wei

Michigan Tech Publications

Auxin is a key regulator that virtually controls almost every aspect of plant growth and development throughout its life cycle. As the major components of auxin signaling, auxin response factors (ARFs) play crucial roles in various processes of plant growth and development. In this study, a total of 35 PtrARF genes were identified, and their phylogenetic relationships, chromosomal locations, synteny relationships, exon/intron structures, cis-elements, conserved motifs, and protein characteristics were systemically investigated. We also analyzed the expression patterns of these PtrARF genes and revealed that 16 of them, including PtrARF1, 3, 7, 11, 13–17, 21, 23, 26, 27, 29, 31, …


Single-Leg Cycling To Maintain And Improve Function In Healthy And Clinical Populations, C. Eric Heidorn, Steve Elmer, Kyle W. Wehmanen, James C. Martin, John Mcdaniel Jan 2023

Single-Leg Cycling To Maintain And Improve Function In Healthy And Clinical Populations, C. Eric Heidorn, Steve Elmer, Kyle W. Wehmanen, James C. Martin, John Mcdaniel

Michigan Tech Publications

Exercise with reduced muscle mass facilitates greater muscle-specific adaptations than training with larger muscle mass. The smaller active muscle mass can demand a greater portion of cardiac output which allows muscle(s) to perform greater work and subsequently elicit robust physiological adaptations that improve health and fitness. One reduced active muscle mass exercise that can promote greater positive physiological adaptations is single-leg cycling (SLC). Specifically, SLC confines the cycling exercise to a smaller muscle mass resulting in greater limb specific blood flow (i.e., blood flow is no longer “shared” by both legs) which allows the individual to exercise at a greater …


Optimized Production Of Second-Generation Bioethanol From A Spent C4 Grass: Vetiver (Chrysopogon Zizanioides), Sameer Neve, Dibyendu Sarkar, Zhiming Zhang, Rupali Datta Dec 2022

Optimized Production Of Second-Generation Bioethanol From A Spent C4 Grass: Vetiver (Chrysopogon Zizanioides), Sameer Neve, Dibyendu Sarkar, Zhiming Zhang, Rupali Datta

Michigan Tech Publications

Vetiver grass (Chrysopogon zizanioides) is well-known for its contaminant phytoextraction potential and its capacity to reduce soil erosion, owing to its massive, dense root system. However, the shoots are not major contributors to either of these processes, and are either not utilized at all or they become part of the waste stream. It is well-recognized that lignocellulosic biomass can serve as a source of raw material to produce second-generation bioethanol. This study investigated the simultaneous saccharification and fermentation (SSF) of acid–alkali pretreated vetiver (VG) shoots by Saccharomyces cerevisiae. Vetiver shoots were obtained from three sources: (1) shoots from VG grown …