Life Sciences Commons^™

Effects Of Main-Stem Impoundments On Hydrology And Larval Fish Communities In Major Tributaries Of Two Large Midwestern Rivers, Jordan Pesik

Masters Theses

Anthropogenic modifications, like impoundments, have altered natural environmental conditions in most lotic systems and impacted fish ecology in many ways. We examined the effect of large river impoundments on fish reproductive behavior by studying the larval fish communities in tributaries of the impounded Illinois and unimpounded Wabash Rivers. We hypothesized that larval fish communities would be similar between the geographically proximal systems, but temporal structuring would depend largely on flow regimes. Biweekly larval densities were greatest among all taxa in the Wabash system, though overall communities were similar between systems. Of the six tributaries, the four smallest rivers were most …

An Efficient Protocol For Root Studies In The Common Sunflower Using Composite Plants, Tyler Parks

Masters Theses

Sunflower, Helianthus annuus, is one of the most important oilseed crop in the world. Oil from the seeds is prized for its' exceptional quality and flavor. Despite this, sunflowers have been forced onto marginal lands often on semi-arid and non-fertile soil, due to disease pressures and economics making it critical to produce more resilient lines and varieties to withstand these new stresses. The recent availability of the sunflower genome can allow genome-wide characterization of gene families. Stable transformation protocols, which can be used for characterization studies, have been developed for H. annuus using Agrobacterium tumefaciens, but they produce …

Resolution Of The Nutritional Requirements And Metabolic Profile Of Clostridium Scindens Atcc 35704, A Major Bile Acid-Dehydroxylating Anaerobe In The Human Gut, Rachana Shrestha

Masters Theses

Bile acids (cholate and chenodeoxycholate) are synthesized from cholesterol in the human liver, and secreted in small intestine for food digestion. However, a portion of these primary bile acids is converted in the colon to toxic secondary bile acids, deoxycholate and lithocholate. Secondary bile acid formation is the result of 7α-dehydroxylating anaerobes, and one of the key players in this conversion is Clostridium scindens. Interestingly, little is known about the basic physiology and nutrition of C. scindens. Therefore, the goal of this research was to understand the nutritional requirement, and determine the 7α-dehydroxylation activity and end products from …

Reproductive Health And Morphometric Comparison Of Catostomid Species In Two Midwestern Rivers, Bethany Ellen Hoster

Masters Theses

Wastewater treatment effluent, agricultural runoff, and impoundments can alter the water quality and flow regime of rivers. Fishes around the world are increasingly exposed to estrogenic compounds due to the discharge of wastewater into rivers. Estrogenic compounds, such as those found in birth control pills and soybean byproducts, are endocrine disruptors in vertebrates. The Sanitary District of Decatur serves the municipal and industrial entities of Macon County, IL, and discharges its effluent into the Sangamon River, which includes byproducts of industrial soybean processing. I assessed impacts of treated wastewater on the health of fishes in the Sangamon River. Abnormal caudal …

Investigation Of A Putative Membrane-Bound Protein In Arabidopsis Thaliana And Nicotiana Tabacum, Allen Potter

Masters Theses

The NLG (Novel Leaf Gene) is a recently discovered gene from aspen (Populus tremula x alba). Previous research indicates that NLG impacts leaf shape. Our preliminary analyses indicate that NLG may function as a membrane bound protein with possible localization in plasma membrane and/or nucleus. Sequence and phylogenetic analyses in Arabidopsis thaliana and Populus trichocarpa identified four homologous genes in Arabidopsis and five in Populus. Gene expression analysis was done using online tools and indicated that NLG is expressed in multiple tissue types but predominately in stem and flowers. The protein coding region of the NLG orthologs genes from aspen …

The Role Of Prrx1 And Snai2 As Master Regulators Of Fibroblast Identity, Huda A. Alzahrani

Masters Theses

Mammalian development involves a complex system of regulatory signals and reactions, leading to the creation of distinct tissues with specific structures and functions controlled by various gene expression profiles. Tissue-specific gene expression is by controlled specific combinations of transcription factors and cofactors that dictate cell differentiation via activation and repression of genes in mammalian cells.

Whole genome microarray analyses of fibroblasts have revealed candidate genes that may serve as master regulators of fibroblast identity, such as Prrx1 and Snai2, that activate downstream genes such as Bmp3, Twist, Shox2, Cfos, Slug, Sema3A, Spp1, and Col1a1. A previous study showed that Prrx1 …

The Effects Of Two Different Sports Drinks On Rpe And Finishing Speed Of An Endurance Run In Highly Trained Distance Runners, Matthew J. Feldhake

Masters Theses

The purpose of this study was to compare the effects of a protein-containing sport drink with a traditional non-protein sport drink has on ratings of perceived exertion (RPE) and finishing speed of an half marathon. Of the possible factors that would support including protein in a sport drink the primary one was its inhibitory effect on serotonin release in the brain. This could help attenuate perceived exertion, thus allowing individuals to compete at a higher level for longer durations (Davis, Alderson, & Welsh, 2000).

This study was conducted using nine members of the Eastern Illinois University Men's Cross Country team. …

The Effect Of Self-Recognition In The Competition Between Genets Of Solidago Altissima, Charles Jaques

Masters Theses

Competition is a major driving force in the abundance, distribution, and diversity within any biological system. Plants are more likely to suffer reductions in fitness as a consequence of competition, as they typically occur in dense communities. Individuals within these populations must respond to both inter- and intraspecific competition. Recent studies suggest that the ability to distinguish kin from non-kin may play an integral role in the success of individuals within different plant populations and communities. However, a less known interaction is that between individual clones within a genet as they grow, spread, and begin to interact with nearby rival …

Evaluation And Demographic Response Of The Shovelnose Sturgeon Commercial Caviar Fishery In The Wabash River, Jessica L. Thornton

Masters Theses

Shovelnose sturgeon (Scaphirhynchus platorynchus Rafinesque) are considered one of the last commercially viable options for sturgeon roe harvest. Due to the collapse of several marine sturgeon fisheries and the Similarity of Appearances provision which protect shovelnose sturgeon in only a part of their range, the caviar fishery in the Wabash River remains an important point of supply for this lucrative natural resource. In this thesis, I presented an evaluation of the shovelnose sturgeon commercial caviar fishery in the Wabash River. More specifically, I described the demographic response of this population to continued exploitation over a 10-year period. Additionally, I …

Impact Of Giant Miscanthus (Miscanthus X Giganteus) Cultivation On Midwestern Farmland Birds, Matthew Craffey

Masters Theses

Giant Miscanthus (Miscanthus x giganteus) is a promising bioenergy feedstock, newly introduced to North America. However, the qualities that make Giant Miscanthus an attractive bioenergy feedstock may pose challenges to local wildlife. To assess the impacts of Giant Miscanthus on Midwestern farmland birds, I conducted point count and vegetation surveys at three sites in east-central Illinois where Giant Miscanthus was being cultivated. I used occupancy modeling to assess the relative influence of Giant Miscanthus on five species relative to other habitat and landscape characteristics. Dickcissel (Spiza Americana), Eastern Meadowlark (Sturnella magna), and Red-Winged Blackbird …

Biotransformation Of Oxo-Bile Acids Derivatives By Human Gut Bacteria, Laina Sallam

Masters Theses

Bile acids are one group of steroids that can be metabolically transformed by human gut anaerobes. One of these transformations is the conversion of primary bile acids to secondary bile acids by 7α-dehydroxylating gut bacteria such as Clostridium scindens, Clostridium hylemonae, and Clostridium hiranonis. These anaerobes metabolize bile acid derivatives via 3α- and 7α-hydroxysteroid dehydrogenases (HSDH). Studies to date have not reported if these organisms are capable of converting 12-oxolithocholic acid (12-oxoLCA) to deoxycholic acid (DCA). The emphasis of this study was to determine the ability of C. scindens ATCC 35704, C. hiranonis DSM 13275, and C. …

Comparative Toxicology Of Nio And Ni(Oh)₂ Nanoparticles, Melissa Hope Cambre

Masters Theses

“Understanding the potential toxicity of nanoparticles (NPs) is important to ensure that these new products do not impose harmful effects to human and environmental health. Paper I is a literature review in which we discuss characteristics of nanomaterials, with an emphasis on transition metal oxide nanoparticles that influence cytotoxicity. Identification of those properties may lead to the design of more efficient and safer nanosized products for various industrial purposes and provide guidance for assessment of human and environmental health risk. We then investigate biochemical and molecular mechanisms of cytotoxicity that include oxidative stress-induced cellular events and alteration of the pathways …

Fluid Flow In A Krogh Cylinder: A Model For A Single Capillary And Surrounding Tissue, Xianjie Qiu

Masters Theses

"A model describing the flow of plasma from the capillaries to the interstitial space in the extravascular tissue and back into the capillaries is constructed and solved. The flow through the tissue or the interstitial spaces is described by the Brinkman equation for porous medium, modified to account for the presence of cells considered as spheres. The flow of blood through the capillaries is considered to be that of a Newtonian fluid. The flow between capillary and tissue is coupled by the permeability of the capillary wall. The nature of flow and its magnitude was found to be highly dependent …

Untangling The Effects Of Biosynthetic And Metabolic Rates On Oxidative Cellular Damage In An Insect Model, Nikki Jihui Gomez

Masters Theses

“Previously, a theoretical model for unravelling the entwined effects of metabolic and biosynthetic rates on oxidative cellular damage accumulation during an animal’s growth period was developed and tested in hornworms (Manduca sexta larvae). In theory, if an animal had unrestricted amounts of energy and a high repair efficiency, they could repair most of the oxidative cellular damage accumulated, regardless of how their metabolic rate varies. However, organisms have a finite amount of energy that is allocated to repair and, during the animal’s development period, a considerable amount of energy goes towards growth eliciting a tradeoff between biosynthesis and repair. …