Life Sciences Commons^™

Spatial And Temporal Patterns Of Bald Eagle (Haliaeetus Leucocephalus) Winter Habitat Use Along The Upper Mississippi River Corridor, Ryan T. Schmitz

Master's Theses

From March 1999 through February 2006, satellite transmitters were placed on 13 wild-caught individuals of the Bald Eagle (Haliaeetus leucocephalus) in southwestern Wisconsin. These individuals (6 males, 7 females, 11 adults, 2 immatures) were tracked along the Upper Mississippi River corridor during the winters of 1999 through 2006. The objectives of my study were to: 1) estimate winter home range size and to compare home range size between sexes, 2) characterize and quantify winter night roost habitat, and 3) identify spatial and temporal patterns of winter habitat use and behavior relative to temperature, wind speed, and ice-cover. Mean 95% fixed …

Flooding Tolerance Of Native And Nonnative Grasses: Variation In Photosynthesis, Transpiration, Respiration, And Carbon Isotope Discrimination, Elizabeth Waring

Master's Theses

Invasion by nonnative plants is particularly prevalent in wetlands. While the ecological patterns in wetland plants are well known, it is less well known how flooding-related soil conditions influence the physiological success of introduced species in wetlands. In chapter 1, effects of flooding were measured in invasive common reed (Phragmites australis), reed canary grass (Phalaris arundinacea), Johnson grass (Sorghum halepense), and native prairie cordgrass (Spartina pectinata). The four species were kept at four levels of flooding (deep flooding, medium flooding, low flooding, and dry conditions), and their responses were measured after 7 and 28 days of treatment using by a …

Differences In Aerobic Response To Wheelchair Locomotion, David Pomfret

Master's Theses

The purpose of this study was to explore the differences in the aerobic response to wheeling between wheelchair dependent individuals and able-bodied individuals of similar genders and ages. Five wheelchair dependent men (WC) and five able-bodied men (AB) performed a 13 minute wheeling test (5 min. at rest, 8 min. wheeling) at 4.0 km∙hr-1. Heart rate (HR) and VO2 were recorded using a Vmax ST system during the constant speed test. There was no significant difference in HR or VO2 between the two groups during rest. Both HR and VO2 were higher for WC during exercise. The mean METS during …

Establishing Buffalograss In Fine Fescue Turfgrass On The Central Coast Of California, Brittani Jean Axtell

Master's Theses

Buffalograss [Buchloe dactyloides (Nutt.) Engelm.] is a warm season, perennial grass native to the Great Plains from southern Canada to Mexico (Beetle, 1950). This newly developed, low input, turf-type grass is recommended for use on low maintenance sites (Falkenberg-Borland and Butler, 1982; Pozarnsky, 1983; Wu and Harivandi, 1989; Shearman et al., 2005). Recently, the use of buffalograss as a turfgrass has increased due to its drought tolerance, low nutrient requirements, and low growing height (Harivandi and Wu, 1995; Frank et al., 2004). It is an excellent choice in California where water use is limited. Unsightly winter dormancy of buffalograss …

Consumers And Benefits Of Genetically Modified Vegetables, Megan Carter Judge

Master's Theses

With the adoption of biotechnology in many agricultural products with first-generation biotechnology traits such as increased pest resistance, greater herbicide resistance, and increased yields the growers have accepted them. The next wave of biotech crops have second-generation traits, such as improved nutrient content, extended shelf life, reduced pesticide and herbicide application (a consumer demanded trait), and better taste. Will these consumer benefits offset any concern that the consumer has regarding biotechnology? What are those benefits and how should the information be communicated to the consumer?

Three focus groups give insight to the proposed questions. The focus groups were done in …

Temperature Affects Adhesion Of The Acorn Barnacle (Balanus Amphitrite), Laurel A. Johnston

Master's Theses

Biofouling is the accumulation of sessile marine organisms, such as algae, tube worms and barnacles on man-made substrata and has negative economic and ecological implications. Ship hulls are readily fouled, which significantly increases drag while decreasing ship fuel efficiency when moving through water. Fouled hulls have also become important vectors of invasive species. These problems are minimized when hulls are painted with a toxic anti-fouling or non-toxic foul-release coating. Due to recent restrictions of anti-fouling paint use, research and development of non-toxic alternatives has increased.

Novel hull coating efficiency is often quantified by the critical removal stress value of barnacles …

Accuracy Of Forest Road And Stream Channel Characteristics Derived From Lidar In Forested Mountain Conditions, Russell Alan White

Master's Theses

Forest roads and stream channels are mapped using a variety of remote sensing and ground-based techniques. In densely forested areas, conventional remote sensing methods provide limited terrain information, while ground-based surveys can be time-consuming, difficult, and expensive. Light Detection and Ranging (LiDAR) is an airborne remote sensing technology used to create high-resolution digital elevation models (DEMs) of the earth’s surface. This study tests the accuracy of forest road and stream channel features mapped using LiDAR in the steep, forested terrain of California’s Santa Cruz Mountains. A conventional total station survey was used to determine centerline position and elevations along a …

The Presence Of Micropterus Salmoides (Largemouth Bass) Influences The Populations Of Rana Draytonii (California Red-Legged Frog) And Pseudacris Regilla (Pacific Treefrog) In Two Ponds In Santa Barbara County, California, Kenneth Lee Gilliland

Master's Theses

Alien fish have been implicated in the decline of Rana draytonii (California red-legged frog) and Pseudacris regilla (Pacific treefrog) populations. Micropterus salmoides (largemouth bass) is a common sport fish that has been introduced into the sympatric range of these two anuran species; however, no studies have investigated the individual influence that this alien fish has on these two species. I conducted M. salmoides control or eradication experiments during a four year study in two ponds within the Transverse Mountain Range of Santa Barbara County, California. Changes in the densities of all life stages of R. draytonii and P. regilla were …

Fish Assemblage Structure In Natural, Channelized, And Restored Sections Of Nippersink Creek, Mchenry County, Illinois, Sarah Ann Zack

Master's Theses

Degradation of aquatic habitats and loss of biodiversity are growing concerns of natural resource managers and the general public. Channelization, the common historical practice of straightening streams and rivers for agricultural interests, has had profound detrimental effects on the biodiversity of lotic fish assemblages. Nippersink Creek, McHenry County, IL is a twenty-three mile stream that flows through an area valued for its fish, wildlife, and invertebrate biodiversity. Although a portion of the stream was channelized in the 1950's, restoration efforts by the McHenry County Conservation District have recently restored historical meanders. Nevertheless, efforts to restore streams and rivers to their …

Temporal Variability Of Bioassessment Indices Used To Evaluate Three Midwestern Streams, Nia M. Haller

Master's Theses

Population, community and functional measures or metrics in rapid bioassessment programs aid in establishing biological criteria for streams and rivers. Each metric measures different aspects of community structure and is important in detecting changes in macroinvertebrate community structure that are influenced by changes in water quality. In this study, temporal variation of nine commonly used bioassessment indices was examined in three midwestern streams. The indices were calculated for each of nine replicate benthic macroinvertebrate samples collected monthly for one year from Cowpie Creek (CC), Nippersink Creek (NC) and Lawrence Creek (LC), McHenry County, Illinois. In practice, the habitat sampled for …

Diet Overlap And Competition Among Native And Non-Native Small-Bodied Fishes In The Colorado River, Grand Canyon, Arizona, Sarah Ellen Zahn Seegert

Master's Theses

Introductions of non-native fishes are threatening native fish communities in streams and rivers across the United States. The Colorado River especially has experienced numerous species invasions, and native fish populations throughout the basin are in decline. The native fish community in the Grand Canyon has been particularly affected, with half of the native fishes extirpated from the canyon. Many scientists blame interactions with non-native fishes for these declines. However, to date no one has conducted a thorough diet analysis of small-bodied fishes in the system to assess overlap between native and non-native fish diets. I analyzed the diets of native …

Resource Composition And Macroinvertebrate Resource Consumption In The Colorado River Below Glen Canyon Dam, Holly Ann Alfreda Wellard Kelly

Master's Theses

Physical and biological changes to rivers induced by large dams can significantly alter downstream communities, decreasing the biotic integrity of these rivers. For example, completion of Glen Canyon Dam on the Colorado River in 1963 has altered the downstream ecosystem and contributed to the decline of native fish populations and dramatic changes in the macroinvertebrate communities. Physical changes associated with the dam may also influence the food resources supporting macroinvertebrate production, but this has not been previously measured. For example, autochthonous production is high in the clear tailwaters of the dam, but downstream tributary allochthonous carbon inputs are substantial and …

Structure-Function Relationship Studies Of The Udp-Glucose Pyrophosphorylase From Escherichia Coli, Agnieszka Maria Orlof

Master's Theses

UDP-Glucose Pyrophosphorylase (UDP-Glc PPase) is a key enzyme of the carbohydrate metabolic pathway widely used among prokaryotes and eukaryotes. In plants, UDP-Glc PPase is necessary for sucrose synthesis while mammals utilize this enzyme for the production of glycogen. A bacterium such as Escherichia coli uses UDP-Glc PPase for biosynthesis of the lipopolysscharide core which forms a cell wall.

This study focused on UDP-Glc PPase from Escherichia coli which is encoded by both galU and galF genes. The first part of this project investigated amino acids that could play an important role in the function of UDP Glc PPase (GalU). Based …

Functional And Phenotypical Analysis Of The Effects Of Aging On B Cells And Their Bone Marrow Microenvironment, Nicole May Ziegler

Master's Theses

Aging impacts multiple organ systems, and specifically causes the immune system to lose its ability to efficiently fight off infections. Regarding immunity, aging research predominantly focuses on the adaptive immune system. B cells, which mediate the humoral arm of the adaptive immune system, develop throughout life in the bone marrow where microenvironmental `niches' are important. The bone marrow does not exactly `atrophy' with age; however, studies comparing young and old mice demonstrate an age-related change in the bone marrow B cell subpopulations.

The overall goal was to determine if femoral and sternum bone marrow have different plasma cell composition and …

The Distribution Of Gnrh And Erbeta In Pre- And Post-Pubertal Male Rats, Maysaa Nageeb

Master's Theses

The hypothalamic-pituitary- gonadal axis is intrinsic in regulating reproductive function. Each part orchestrates the synthesis and release of a key hormone. At the level of the hypothalamus, Gonadotropin- releasing hormone (GnRH) is released and it is the

most upstream regulator of sexual maturation. GnRH neurons express a type of estrogen receptor, ERbeta, which suggests that estradiol (E2) plays a direct role in modulating GnRH function (Herbison and Pape 2001). The objective of this study was to determine if gonadal steroid hormones regulate the expression of ERbeta and GnRH mRNA in the male brain during pubertal development. In-situ hybridization was used …

Hcnabc Gene Expression In The Soil Bacterium Pseudomonas Putida Ath2-Iri/9 Under Various Culture And Rhizosphere Conditions, Myrna May Biswas

Master's Theses

Pseudomonas putida ATH2-1RI/9, a bacterium that colonizes plant roots, can suppress weed seedling growth of velvetleaf by producing hydrogen cyanide in the rhizosphere. The operon hcnABC constitutively expresses HCN synthase, an enzyme which catalyzes glycine to form carbon dioxide and hydrogen cyanide, a secondary metabolite. Cyanogenesis is influenced by several environmental factors including iron, phosphate, and oxygen-limiting conditions. This study used a hcnABC::luxAB gene fusion in Pseudomonas putida ATH2-1RI/9 involving the insertion of a promoterless luxAB gene into the hcnABC operon, which results in bacterial luciferase expression instead of HCN synthase. Bioluminescence by this reporter strain was compared to cyanide …

Phenotypical And Functional Analysis Of Peripheral T Cells In Foxn1 Transgenic Mice: Effects Of Aging, Paulette Krishack

Master's Theses

The thymus is the primary organ for the development and production of TCRαβ naive T cells. However, with increasing age thymic involution occurs, causing a decline in the output of naïve T cells. The decline in naïve T cell production results in a contraction in the peripheral naïve and expansion of the memory T cell pools. Not only are the production and compositions of peripheral T cells altered with age, T cell functions such as T cell proliferation and production of cytokines required for cell proliferation are also declined. Currently, it is not known if restoring the decline in the …

Localization And Functional Analysis Of Plasmodium Falciparum Genes Pfl2550w And Pff0750w, Carolyn Jane Strobel

Master's Theses

Malaria is a parasitic disease that causes over a million deaths worldwide each year. Understanding development through the parasite's life cycle is necessary to stop disease transmission. As the genetic basis for the crucial transition from the erythrocytic asexual cycle to gametocytogenesis is unknown, we hope to better understand this transition by studying sexual stage genes and their roles in gametocytogenesis. PFL2550w and PFF0750w are genes upregulated during gametocytogenesis that were identified by a whole-genome microarray comparing gene expression between gametocyte-producing and gametocyte-deficient strains. In this study, PFL2550w was shown to be a soluble protein that is exported from the …

Uncultivated Environmental Tm7 Model To Study Human Disease-Associated Tm7 Bacteria, David Barton

Master's Theses

The TM7 bacterial phylum has no cultivated species and includes members that span a broad range of environmental and human habitats, some of which are associated with human periodontitis. In this project, activated wastewater TM7 bacteria were analyzed and their relatedness compared to human-associated TM7 bacteria for the long-term goal of using an environmental TM7 to better understand TM7 pathogenesis in humans. DNA was extracted from activated wastewater and PCR amplified using TM7 16S rRNA gene- specific primers. The ~1,170 base pair PCR products were then cloned and sequenced. DNA sequencing and phylogenetic analysis identified environmental TM7 clones with high …

Courtship And Copulation In Glaucous-Winged Gulls, Larus Glaucescens, Kelly M. Mcwilliams

Master's Theses

Using video recordings and scan counts, I examined the occurrence, sequences, and durations of behavior and environmental factors associated with courtship and copulation in glaucous-winged gulls, Larus glaucescens. Males and females in a pair had equivalent pre- and post-Mount behavior occurrences and durations. The main courtship sequence was Head Toss -- Courtship Beg/Respond -- Mounted/Mount, and felames appeared to initiate transitions between these pre-Mount behaviors. More Mounts occurred during the egg-laying stage of the breeding season, but copulation duration, wing-flag duration, and cloacal contacts per copulation showed no significant differences across sampling periods. Poisson analysis showed no significant grouping …