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Articles 1 - 30 of 87
Full-Text Articles in Life Sciences
Exploring The Potential Of Alcohol-Fermenting Yeast Isolated From Heterotrigona Itama Stingless Bees For Beverage Production, Pimprapa Chaijak, Alisa Kongthong, Chiraprapha Maenpaa
Exploring The Potential Of Alcohol-Fermenting Yeast Isolated From Heterotrigona Itama Stingless Bees For Beverage Production, Pimprapa Chaijak, Alisa Kongthong, Chiraprapha Maenpaa
Makara Journal of Science
Mead, an ancient alcoholic beverage, relies on the indigenous microbial community in honey for alcohol fermentation. Nevertheless, data on the use of bee indigenous flora for mead production in Thailand are scarce. This investigation involved the isolation of alcohol-producing yeast strains from the Thai stingless bee species Heterotrigona itama. Subsequently, these isolated yeast strains were employed in mead fermentation with honey sourced from the same stingless bee species. Findings revealed that the yeast strain denoted as P03b, which was identified molecularly as Saccharomyces cerevisiae, exhibited the highest alcohol production of 13.53% ± 0.76%. Furthermore, the yeast strain P03b …
Synthesis, Molecular Characteristics, And Antibacterial Assessment Of Marine Hydroid Aqueous Extract-Based Silver Bio-Nanoparticles, Fredryk Mandey, Aulia Rhamdani Arfan, Rugaiyah Andi Arfah
Synthesis, Molecular Characteristics, And Antibacterial Assessment Of Marine Hydroid Aqueous Extract-Based Silver Bio-Nanoparticles, Fredryk Mandey, Aulia Rhamdani Arfan, Rugaiyah Andi Arfah
Makara Journal of Science
This investigation aims to synthesize, analyze the molecularity, and test the ability of bacterial inhibition capability of silver nanoparticles that have been synthesized by simply mixing silver nitrate and aqueous extracts of marine natural products with and without the addition of amylum as a stabilizing agent. This research, with and without the addition of amylum as a stabilizing agent, obtained 39.0 and 55.2 mg of solids of round-shaped morphology silver nanoparticles with diameters of 87.9 and 103.0 nm., respectively. In addition, the antibacterial testing assay against Staphylococcus aureus and Escherichia coli showed some considerably good results. S. aureus with the …
Effect Of Harvest Season On The Physical Properties, Fatty Acid Composition, And Volatile Compounds Of Roasted Cacao Beans, Amornrat Chumthong, Nuttarisa Limjumrern, Chutikarn Saensano, Pornthep Teerawattanapong, Aekkaraj Nualla-Ong, Adirek Rugkong, Rawee Chiarawipa
Effect Of Harvest Season On The Physical Properties, Fatty Acid Composition, And Volatile Compounds Of Roasted Cacao Beans, Amornrat Chumthong, Nuttarisa Limjumrern, Chutikarn Saensano, Pornthep Teerawattanapong, Aekkaraj Nualla-Ong, Adirek Rugkong, Rawee Chiarawipa
Makara Journal of Science
The prevailing climate is an important factor affecting the development of the physical quality and flavor of cacao (Theobroma cacao L.) beans. The influence of harvest seasons on the physical, chemical, and flavor characteristics of cacao beans from Nakhon Si Thammarat Province, southern Thailand, was assessed. Among cacao beans, those harvested in the rainy season (R) had the highest fresh and dry weights and the lowest bean count per 100 g (87.00 ± 2.83). Although the overall fat content remained relatively constant across harvest seasons, cacao beans harvested in R exhibited a significantly higher fatty acid (C16:0, C18:1, and …
In Silico Analysis Of C-Type Lectins As Co-Infection Receptors Of Dengue And Chikungunya Viruses In Aedes Aegypti, Munawir Sazali, R. C. Hidayat Soesilohadi, Nastiti Wijayanti, Tri Wibawa, Arif Nur Muhammad Ansori
In Silico Analysis Of C-Type Lectins As Co-Infection Receptors Of Dengue And Chikungunya Viruses In Aedes Aegypti, Munawir Sazali, R. C. Hidayat Soesilohadi, Nastiti Wijayanti, Tri Wibawa, Arif Nur Muhammad Ansori
Makara Journal of Science
Aedes aegypti is a primer vector of dengue virus (DENV) and chikungunya virus (CHIKV). The susceptibility of mosquitoes to DENV and CHIKV depends on their recognition receptor of pathogens. C-type lectins (CTLs) are an important mediator of virus infection in A. aegypti. This study aims to identify potential receptors and determine the binding affinity between ligand–receptor interaction, CTLs and virus envelopes (DENV-1, 2, 3, and 4 and CHIKV) interaction based on in silico analysis. Sample sequences were obtained from GenBank (NCBI), and 10 CTLs were acquired from VectorBase. Homology modeling based on a minimum standard of 20% was processed …
Using In Silico Tools To Analyze The 5ʹ Untranslated Regions Of The Alcohol Dehydrogenase Gene From Arabidopsis Thaliana And Omega Sequence, Reza Mohammadhassan, Tina Asadishad
Using In Silico Tools To Analyze The 5ʹ Untranslated Regions Of The Alcohol Dehydrogenase Gene From Arabidopsis Thaliana And Omega Sequence, Reza Mohammadhassan, Tina Asadishad
Makara Journal of Science
The 5ʹ ends of protein-encoding genes contain 5ʹ untranslated regions (5ʹUTRs), which can effectively participate in regulating gene expression. The 5ʹUTRs of Arabidospis thaliana–derived alcohol dehydrogenase gene (AtADH) and omega (Ω) sequence from tobacco mosaic virus (TMV) are the most effective enhancers in biotechnology. In this study, bioinformatics techniques were employed to analyze the characteristics of the above sequences. After 5ʹUTR sequence collection, the inner ribosome entrance sites; small RNA (sRNA) target sequences; nucleotide contents; and upstream start and stop codons, ORFs, and coding DNA sequences of the Ω sequence and AtADH 5ʹUTR were identified. Moreover, the …
An Inkjet-Printed Graphene Oxide–Poly(3,4-Ethylenedioxythiophene) Poly(Styrene Sulfonate) Electrode For Nitrite Detection In Water, Budi Riza Putra, Weni Anindya, Mohamad Rafi, Ika Kartika, Yudi Nugraha Thaha, Aga Ridhova, Wulan Tri Wahyuni
An Inkjet-Printed Graphene Oxide–Poly(3,4-Ethylenedioxythiophene) Poly(Styrene Sulfonate) Electrode For Nitrite Detection In Water, Budi Riza Putra, Weni Anindya, Mohamad Rafi, Ika Kartika, Yudi Nugraha Thaha, Aga Ridhova, Wulan Tri Wahyuni
Makara Journal of Science
In this study, a screen-printed electrode (SPE) for nitrite (NO2−) sensing was fabricated through an inkjet printing technique using a commercial printer machine and preparing an ink composite solution containing graphene oxide (GO) and poly(3,4 ethylenedioxythiophene) poly(styrene sulfonate) (PEDOT:PSS) as conductive materials. The optimum ink materials for SPE fabrication comprised GO and PEDOT:PSS with binders that resemble the viscosity of commercial ink. The surface topography of the fabricated SPE, which was characterized using scanning electron microscopy and atomic force microscopy, showed a smoother surface compared to the commercial screen-printed carbon electrode, with conductive ink materials deposited primarily …
The Potential Of Endophytic Bacteria As The Biocontrol Agents Of Fusarium Proliferatum, Rima Ernia, Nisa Rachmania Mubarik, Lisdar Idwan Sudirman
The Potential Of Endophytic Bacteria As The Biocontrol Agents Of Fusarium Proliferatum, Rima Ernia, Nisa Rachmania Mubarik, Lisdar Idwan Sudirman
Makara Journal of Science
Fusarium proliferatum decreases the productivity of shallot (Allium cepa L.). Endophytic bacteria are potential biocontrol agents in controlling the growth of F. proliferatum. This study aimed to select and characterize endophytic bacteria producing bioactive compounds that inhibit the growth of F. proliferatum. The endophytic bacteria used were isolated from the roots, stems, leaves, and soil of shallot plants from healthy and diseased shallot farming fields. The endophytic bacteria were selected using the dual culture method and extracted using ethyl acetate solvent. The results of the 16S rRNA identification suggested that isolate ABP5.2 is similar to Pseudomonas …
Free-Radical Scavenging Activity And Total Phenolic Compounds Of Red And Green Poinsettia Leaves (Euphorbia Pulcherrima Willd.) From Lombok Island, Handa Muliasari, Baiq Sopiah, Emmy Yuanita, Baiq Nila Sari Ningsih
Free-Radical Scavenging Activity And Total Phenolic Compounds Of Red And Green Poinsettia Leaves (Euphorbia Pulcherrima Willd.) From Lombok Island, Handa Muliasari, Baiq Sopiah, Emmy Yuanita, Baiq Nila Sari Ningsih
Makara Journal of Science
To determine the free-radical scavenging activity and total phenolic content of the ethanol extract of Poinsettia leaves (Euphorbia pulcherrima Willd.) from Lombok Island, we used the 1,1-diphenyl-2-picrylhydrazyl method and expressed the total phenolic content as gallic acid equivalent (GAE) per gram of ethanol extract. The results show the strong antioxidant activity (79.77 ppm) of the ethanol extract of red Poinsettia leaves and the moderate antioxidant activity of green Poinsettia leaves (118.350 ppm). The ethanol extracts of red and green Poinsettia leaves possessed an average of 63.276 ± 0.228 and 22.715 ± 0.090 mg GAE/g extract total phenolic contents, respectively. …
Enzymatic Screening And Genotypic Characterization Of Thermophilic Bacteria From The Hot Springs Of Sarawak, Malaysia, Seng Chiew Toh, Samuel Lihan, Sui Sien Leong, Azizul Hakim Lahuri, Wai Cheong Woon, Wing Woh Ng
Enzymatic Screening And Genotypic Characterization Of Thermophilic Bacteria From The Hot Springs Of Sarawak, Malaysia, Seng Chiew Toh, Samuel Lihan, Sui Sien Leong, Azizul Hakim Lahuri, Wai Cheong Woon, Wing Woh Ng
Makara Journal of Science
Owing to their eccentric thermostable ability, thermophiles are among the most utilized extremophiles in various industries, such as manufacturing, and clinical research. Researchers believe that many unknown thermophiles are yet to be discovered. This study aimed to genotypically characterize the diversity of thermophiles and screen them for the potential production of enzymes in the recreational hot springs located at Northwest Coast of Borneo. Water samples were collected at 45 °C–50 °C from Annah Rais and Panchor hot springs during the sampling period from January 2018 to January 2019. Three samples (water and sediment) were collected twice in a 3-week interval …
Isolation Of Lignin From Sugarcane Bagasse As An Adsorbent For Chromium Ion, Nila Tanyela Berghuis, Nurul Fitri Novianti, Paramita Jaya Ratri
Isolation Of Lignin From Sugarcane Bagasse As An Adsorbent For Chromium Ion, Nila Tanyela Berghuis, Nurul Fitri Novianti, Paramita Jaya Ratri
Makara Journal of Science
The waste generated in metal coating and leather tanning industries contribute to water pollution owing to the use of Cr metal in production processes. The utilization of lignin from natural base materials in the form of bagasse can reduce unwanted waste from production processes and adsorb Cr ion waste. In this study, lignin was successfully isolated from bagasse waste and then carbonized and applied in Cr metal absorption. Lignin and lignin carbon as an absorbent were characterized using Fourier-transform infrared spectroscopy (FTIR) and Scanning Electron Microscopy (SEM). The ability of bagasse lignin and carbon lignin to absorb Cr ion waste …
Synthesis Of Copper (Ii) Oxide Nanoparticles Using Vitis Vinifera L. Leaf Ex-Tract And Its Application As A Catalyst In Doebner Reaction, Felie Virgayani Kurniawan, Antonius Herry Cahyana, Rika Tri Yunarti
Synthesis Of Copper (Ii) Oxide Nanoparticles Using Vitis Vinifera L. Leaf Ex-Tract And Its Application As A Catalyst In Doebner Reaction, Felie Virgayani Kurniawan, Antonius Herry Cahyana, Rika Tri Yunarti
Makara Journal of Science
Plant extracts can be used to synthesize nanoparticle because it is efficient, economical, and friendly to the environment. Copper oxide nanoparticles (CuO NPs) were synthesized using an aqueous fraction of Vitis vinifera L leaf extract and Cu(NO3)2 solution as a precursor. This fraction contains secondary metabolites as a reduction agent and stabilizing agents to formed CuO NPs. CuO NPs can be used as a catalyst in a chemical reaction, one of which is the Doebner reaction, which is a simple and efficient reaction of aniline, aromatic aldehydes, and pyruvic acid under reflux conditions to produce derivatives quinoline-4-carboxylic …
Newly Recorded Panus Lecomtei (Fr.) Corner (Basidiomycota: Panaceae) For Indonesia, Ivan Permana Putra, Rudy Hermawan, Ali Bin Abithalib Salman
Newly Recorded Panus Lecomtei (Fr.) Corner (Basidiomycota: Panaceae) For Indonesia, Ivan Permana Putra, Rudy Hermawan, Ali Bin Abithalib Salman
Makara Journal of Science
Panus lecomtei (Polyporales) is reported for the first time from Indonesia at the IPB University Campus Forest (IPBUCF). This species has similar macroscopic features to P. strigellus and P. neostrigosus. This study aimed to confirm the taxonomic position of P. lecomtei found at IPBUCF based on morphological and molecular evidence. The specimens were described by their macroscopic and microscopic characteristics. A phylogenetic approach was used with the internal transcribed spacer 1/2 sequence data. The combination of morphological and molecular analyses confirmed the taxonomic position of our specimens as P. lecomtei. The recorded species was distinguished by gregarious basidiomata, an …
Synthesis, Characterization, Antibacterial Activity, And Potential Water Filter Application Of Copper Oxide/Zeolite Composite, Elfa Aida Kamila, Zaenal Abidin, Irma Isnafia Arief, Trivadila Trivadila
Synthesis, Characterization, Antibacterial Activity, And Potential Water Filter Application Of Copper Oxide/Zeolite Composite, Elfa Aida Kamila, Zaenal Abidin, Irma Isnafia Arief, Trivadila Trivadila
Makara Journal of Science
Detection of E. coli bacteria in water samples can indicate the presence of other bacterial contamination in feces. Bacterial contamination can be treated with antibiotics, but excessive use of antibiotics leads to the emergence of multiresistant bacteria. Therefore, alternative antibacterial agents must be explored. Copper ion/zeolite composite has been widely studied as an antibacterial agent. However, the released copper ions accumulate in water and are toxic to humans; this can be prevented by converting copper ions into copper oxides (CuO and Cu2O). In this study, copper oxide/zeolite composite is synthesized and characterized using the heating method, followed by …
Pro-Apoptosis Activity Of Pogostemon Cablin Benth. Against Nasopharyngeal Carcinoma Through The Bcl-2 Inhibition Signaling Pathway: A Computational Investigation, Aigia Syahraini, Essy Harnelly, Feri Eko Hermanto
Pro-Apoptosis Activity Of Pogostemon Cablin Benth. Against Nasopharyngeal Carcinoma Through The Bcl-2 Inhibition Signaling Pathway: A Computational Investigation, Aigia Syahraini, Essy Harnelly, Feri Eko Hermanto
Makara Journal of Science
Resistance to chemotherapy and radiotherapy frequently emerges in the later stage of nasopharyngeal carcinoma (NPC) tumorigenesis. The decreased response of NPC to radiotherapy and chemotherapy occurs owing to the inhibition of cancer cell apoptosis by the B-cell lymphoma-2 (BCL-2) protein. Thus, inhibiting BCL-2 protein may become a powerful approach to eliminate NPC through apoptosis regulation. Meanwhile, Pogostemon cablin is reported to exhibit anticancer properties, but there are limited studies on its use for NPC treatment. The objective of this study is to investigate the potential bioactive compounds in P. cablin as anti-apoptosis BCL-2 protein inhibitors using in-silico approach. Natural compounds …
Chicken Immune Responses To Vaccination By The Avian Influenza Subtype H5n1 Against Avian Influenza, Ramlah Ramlah, Sri Murtini, Retno Damayanti Soejoedono, Ketut Karuni Nyanakumari Natih, Siti Gusti Ningrum
Chicken Immune Responses To Vaccination By The Avian Influenza Subtype H5n1 Against Avian Influenza, Ramlah Ramlah, Sri Murtini, Retno Damayanti Soejoedono, Ketut Karuni Nyanakumari Natih, Siti Gusti Ningrum
Makara Journal of Science
Avian Influenza (AI) is one of the strategic animal diseases still considered a priority for control by the Indonesian government. This study aimed to examine the immune response of chickens to various types of H5N1 subtype AI vaccines in Indonesia and to determine the correlation of factors influencing the post-vaccination AI antibody response. Serum samples were tested using the hemagglutination inhibition (HI) test with the standard AI antigen subtype H5N1 strain A/Chicken/Barru/BBVM/41-13/2013 (Clade 2.1.3) and strain A/Chicken/Semarang/04141225-07/2014 (Clade 2.3.2). Antibody titer was calculated using the Geometric Mean Titer (GMT). The correlation analyses were performed to assess the correlation of antibody …
The Effect Of Acidic Ph On Growth Kinetics, Biomass Productivity, And Prima-Ry Metabolite Contents Of Euglena Sp., Istini Nurafifah, Muhammad Andhi Hardianto, Tia Erfianti, Ria Amelia, Khusnul Qonita Maghfiroh, Dedy Kurnianto, Dwi Umi Siswanti, Brilian Ryan Sadewo, Renata Adaranyssa Egistha Putri, Eko Agus Suyono
The Effect Of Acidic Ph On Growth Kinetics, Biomass Productivity, And Prima-Ry Metabolite Contents Of Euglena Sp., Istini Nurafifah, Muhammad Andhi Hardianto, Tia Erfianti, Ria Amelia, Khusnul Qonita Maghfiroh, Dedy Kurnianto, Dwi Umi Siswanti, Brilian Ryan Sadewo, Renata Adaranyssa Egistha Putri, Eko Agus Suyono
Makara Journal of Science
Euglena is a microalga with the potential to be an environmentally friendly renewable energy resource. The pH value is a crucial factor in micro-algal cultivation. Changes in pH affect the growth and development of microalgae, including the production of biomass and primary metabolites, such as proteins, carbohydrates, and lipids. In this study, Euglena sp. was grown on Cramer-Myers medium and subjected to various acidic conditions. This study aimed to determine the effect of pH on the growth kinetics, biomass, carbohydrate, lipid, and protein contents of Euglena sp. The Euglena sp. culture was optimized at various pH values of 2.5, 3.5, …
Molecular Simulation Of B-Cell Epitope Mapping From Nipah Virus Attachment Protein To Construct Peptide-Based Vaccine Candidate: A Reverse Vaccinology Approach, Viol Dhea Kharisma, Farida Aryani Dian, Pavel Burkov, Pavel Scherbakov, Marina Derkho, Ekaterina Sepiashvili, Teguh Hari Sucipto, Arli Aditya Parikesit, Ahmad Affan Ali Murtadlo, Vikash Jakhmola, Rahadian Zainul
Molecular Simulation Of B-Cell Epitope Mapping From Nipah Virus Attachment Protein To Construct Peptide-Based Vaccine Candidate: A Reverse Vaccinology Approach, Viol Dhea Kharisma, Farida Aryani Dian, Pavel Burkov, Pavel Scherbakov, Marina Derkho, Ekaterina Sepiashvili, Teguh Hari Sucipto, Arli Aditya Parikesit, Ahmad Affan Ali Murtadlo, Vikash Jakhmola, Rahadian Zainul
Makara Journal of Science
There are no specific drugs or vaccines for Nipah virus (NiV), which is a new Paramyxovirus that infects swine and humans. This study was conducted to investigate B-cell epitope mapping of the NiV attachment glycoprotein and to construct peptide-based vaccine candidates using the reverse vaccinology approach. To generate the linear B-cell epitope, the NiV isolates were extractad from GenBank, NCBI, using the IEDB web server; peptide modeling was conducted using PEP-FOLD3; docking was conducted using PatchDock and FireDock; and in silico cloning was designed using SnapGene. Various peptides were successfully identified from the NiV attachment glycoprotein based on B-cell epitope …
Application Of Photoacoustic Imaging For Pneumonia Detection, Caesarany Maqfiroh, Rini Widyaningrum, Ahmad Mujtahid Anas, Mitrayana Mitrayana
Application Of Photoacoustic Imaging For Pneumonia Detection, Caesarany Maqfiroh, Rini Widyaningrum, Ahmad Mujtahid Anas, Mitrayana Mitrayana
Makara Journal of Science
We used photoacoustic imaging (PAI) to visualize and compare acoustic intensity levels in pneumonia-affected and healthy chicken lungs. After histological confirmation of pneumonia, the samples were scanned and subjected to a 532-nm diode laser in a photoacoustic imaging system. The acoustic intensity level of pneumonia-affected tissue was examined and compared with that of healthy lung samples. The optimum laser frequency and duty cycle for imaging the samples were 17 kHz and 30%, respectively. The acoustic intensity levels of pneumonia-affected tissue and healthy lungs were −82.5 ± 1.8 dB and −79.9 ± 1.3 dB, respectively. We found that a simple PAI …
Physicochemical Changes And Role Of Analytical Chemistry In Black Garlic (Allium Sativum L.) Processing, Surjani Wonorahardjo, Dian Puspita Sari, Arini Salsabila, Estiyawati Estiyawati, Dewi Yuliani, Anugrah Ricky Wijaya, Suharti Suharti, Irma Kartika Kusumaningrum, Chariztya Anggita Maharani, Thjiong Angelina Noviyanti
Physicochemical Changes And Role Of Analytical Chemistry In Black Garlic (Allium Sativum L.) Processing, Surjani Wonorahardjo, Dian Puspita Sari, Arini Salsabila, Estiyawati Estiyawati, Dewi Yuliani, Anugrah Ricky Wijaya, Suharti Suharti, Irma Kartika Kusumaningrum, Chariztya Anggita Maharani, Thjiong Angelina Noviyanti
Makara Journal of Science
Black garlic is a traditional spice having potential of herbal medicine; however, continuous heating darkens its color and changes its taste due to chemical processes and new equilibrium in the system. In this study, fresh garlic was processed into black garlic at 60 °C for 24 days. Changes in physical and chemical parameters, such as browning and protein content, reducing sugar content, and antioxidative components were recorded. Protein content was measured by Kjeldahl method, brown color development was analyzed using a color reader, reducing sugar was examined using a dinitro salicylic DNS reagent, and antioxidative activities were studied with a …
Nutrients Ions Indices Of Some Medicinal Flora Of Cholistan Desert Of Pakistan, Ghulam Yasin, Adeela Altaf, Ikram Ul Haq, Samra Nazeer, Mubasharah Sabir
Nutrients Ions Indices Of Some Medicinal Flora Of Cholistan Desert Of Pakistan, Ghulam Yasin, Adeela Altaf, Ikram Ul Haq, Samra Nazeer, Mubasharah Sabir
Makara Journal of Science
By knowing the nutritional status of a plant, one can better manage and use it for ethnobotanical purposes. Various nutrients are accumulated as osmotica in plants of stressful environments. The type and quantity of nutrients present in plants also determine the effectivity of medicine obtained from that plant. Hence, practical utilization of these plants as food or medicines needs to explore the exact nature and presence of the nutrient ions for discrimination of their toxic or medicinal nature. In the present study, some important nutrients in a number of xerophytic plants from Cholistan desert of Pakistan were quantified. Leave, stem …
Enhancing Lovastatin Biosynthesis In Oyster Mushrooms (Pleurotus Ostreatus) Using Phytohormones, Fransisca Astrid Mustafa, Mamat Kandar, I Nyoman Pugeg Aryantha
Enhancing Lovastatin Biosynthesis In Oyster Mushrooms (Pleurotus Ostreatus) Using Phytohormones, Fransisca Astrid Mustafa, Mamat Kandar, I Nyoman Pugeg Aryantha
Makara Journal of Science
The biosynthesis of lovastatin, the anti-cholesterol compound, in oyster mushrooms (Pleurotus ostreatus), has the potential to be enhanced by utilizing phytohormones, which activate the expression of certain genes. This research aimed to determine the best type and concentration of phytohormone among auxin, gibberellin, and cytokinin, as well as the best mycelial colonization percentage in F2 medium to realize the greatest increase in lovastatin biosynthesis in oyster mushrooms. Lovastatin was extracted from the fruiting bodies and mycelia and analyzed by spectrophotometry. The analysis of CYP450 linked to lovastatin biosynthesis was conducted by quantitative polymerase chain reaction (qPCR) using samples containing …
The Potency Of Citrus Aurantiifolia Swingle And Sea Salt Solution As A Cleansing Agent For Edible Bird’S Nests, Siti Gusti Ningrum, Adhitya Yoppy Ro Candra, Hana Cipka Pramuda Wardhani
The Potency Of Citrus Aurantiifolia Swingle And Sea Salt Solution As A Cleansing Agent For Edible Bird’S Nests, Siti Gusti Ningrum, Adhitya Yoppy Ro Candra, Hana Cipka Pramuda Wardhani
Makara Journal of Science
The present study aimed to evaluate the potency of Citrus aurantiifolia Swingle and sea salt solution as a promising cleansing agent for edible bird’s nests (EBNs). Ascorbic acid, sodium, and chloride analyses of the C. aurantiifolia Swingle and sea salt solution were conducted using HPLC, ICP-OES, and titrimetry, respectively. The characteristics of physical samples and hydrogen peroxide detection were examined in this study. The reduction activity of the Citrus aurantiifolia Swingle and sea salt solution on the nitrite of 120 white EBNs was studied. This nitrite reduction activity was compared with the available method (standard), drinking water method, 6% C. …
Antibacterial Activity Of Honeybee Venom Against Pathogenic Bacteria In Comparison With Common Antibiotics, Shahin Gavanji, Azizollah Bakhtari
Antibacterial Activity Of Honeybee Venom Against Pathogenic Bacteria In Comparison With Common Antibiotics, Shahin Gavanji, Azizollah Bakhtari
Makara Journal of Science
Antibiotic resistance has dramatically spread in recent decades and has become a serious problem in modern medicine. Honeybee venom or bee venom (BV) has anticancer, anti-inflammation, and antimicrobial properties. This study aimed to evaluate the influence of BV on certain pathogenic bacteria. The effects of different concentrations of BV against Klebsiella pneumonia, Pseudomonas aeruginosa, and Staphylococcus aureus were evaluated by disk diffusion method (inhibition zone, IZ), minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). Additionally, the antibacterial activity of BV was compared with that of ampicillin, penicillin, and tetracycline. The results indicated that different concentrations of BV had …
Enrichment Media Selection And Co-Culture Potential Among Exoelectrogen Bacteria Vary With Ecological Factors, Nur Syafira Khoirunnisa, Syaiful Anwar, Untung Sudadi, Dwi Andreas Santosa
Enrichment Media Selection And Co-Culture Potential Among Exoelectrogen Bacteria Vary With Ecological Factors, Nur Syafira Khoirunnisa, Syaiful Anwar, Untung Sudadi, Dwi Andreas Santosa
Makara Journal of Science
Staphylococcus saprophyticus ICBB 9554 and Citrobacter freundii ICBB 9763 are exoelectrogen bacteria applied as microbial fuel cells (MFC). We selected enrichment media for both these exoelectrogen bacteria, characterized their synergistic traits, and evaluated the growth conditions under different ecological factors. In this study, different enrichment media, such as those containing sugar, molasses, and palm sugar (2, 4, and 6% w/v) were tested for S. saprophyticus ICBB 9554. Meanwhile, technical sodium acetate (NaAc), commercial vinegar 25%, and cider vinegar (5, 10, and 15 mM acetate concentration) were tested for C. freundii ICBB 9763. Both the exoelectrogen bacteria were tested for the …
Modification Of Zeolite Made From Coal Fly Ash With Tio2: Effect Of Aging Time On Physical And Optical Properties, Linda Jati Kusumawardani, Ani Iryani, Ennita Lusiana Sinaga
Modification Of Zeolite Made From Coal Fly Ash With Tio2: Effect Of Aging Time On Physical And Optical Properties, Linda Jati Kusumawardani, Ani Iryani, Ennita Lusiana Sinaga
Makara Journal of Science
The high levels of metal oxides, SiO2, and Al2O3 give coal fly ash the potential for development into zeolite. Zeolite was prepared from fly ash by combining alkaline fusion with hydrothermal treatment. Using this method results in a high surface area, high mechanical properties, and high crystallinity of the sample. Zeolite as a product is modified by TiO2. This study was intended to determine the effect of the operating process in zeolite synthesis from coal fly ash on the types and properties of zeolite and the effect of the aging time of TiO …
Synthesis And Study Of Anticancer And Antioxidant Activities Of 2-Oxoquinoline Hydrazide Derivatives, Marwa Khalil Mohamed, Ezzeldin Mohamed S. Salem, Ibrahim Ahmed I. Ali
Synthesis And Study Of Anticancer And Antioxidant Activities Of 2-Oxoquinoline Hydrazide Derivatives, Marwa Khalil Mohamed, Ezzeldin Mohamed S. Salem, Ibrahim Ahmed I. Ali
Makara Journal of Science
In this work, hydrazone, thiosemicarbazide, diamide, and dipeptide derivatives were prepared from the glycine and β-alanine hydrazide of 4-hydroxy-2-oxoquinoline. The desired derivative resulted from substitution, hydrazinolysis, condensation, and azide coupling reactions. The fabricated derivatives were screened for anticancer characteristics toward human’s colon carcinoma (HCT-116) cell line using the 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide viability assay, while antioxidant property was examined using an in vitro H2O2 scavenging method. The β-alanine hydrazide derivative 3a showed a remarkable IC50 value of 18.8 μg/mL, whereas its corresponding thiosemicarbazide 5b exhibited moderate activeness with an IC50 value of 26.89 μg/mL. Conversely, …
Diketopiperazine Cyclo-(S-Pro-R-Leu) Produced By Periconia Pseudobyssoides K5 Isolated From Toona Sureni (Meliaceae) And Its Heme Polymerization Inhibition Activity, Azmi Azhari, Desi Harneti, Asri Peni Wulandari, Yeni Mulyani, Sari Purbaya, Aprilia Permata Sari, Galih Bayu Pratama, Unang Supratman, Yoshihito Shiono
Diketopiperazine Cyclo-(S-Pro-R-Leu) Produced By Periconia Pseudobyssoides K5 Isolated From Toona Sureni (Meliaceae) And Its Heme Polymerization Inhibition Activity, Azmi Azhari, Desi Harneti, Asri Peni Wulandari, Yeni Mulyani, Sari Purbaya, Aprilia Permata Sari, Galih Bayu Pratama, Unang Supratman, Yoshihito Shiono
Makara Journal of Science
Fungi endophytes are living microorganisms colonizing inside the internal tissue of a plant and provide ecological benefits for their host. Endophytes provide various metabolites for plant adaptation toward biotic and abiotic stresses and have tremendous pharmacological activities. Toona sureni (T. sureni) (Meliaceae) belongs to the Toona genera and is reported to have antimalarial activity. To determine the compounds produced by the endophytic fungus from this plant, we isolated a compound from Periconia pseudobyssoides (P. pseudobyssoides) K5 endophytes from the stem bark of T. sureni. Diketopiperazine cyclo-(S-Pro-R-Leu), a non-ribosomal peptide, was isolated from brown …
Potential Effect Of Phlorotannins In Brown Algae For Antidiabetic Therapy: Molecular Docking Approach, Melani Sinastri Renagupita, Rahadianto Rahadianto, Efyluk Garianto, Herin Setianingsih
Potential Effect Of Phlorotannins In Brown Algae For Antidiabetic Therapy: Molecular Docking Approach, Melani Sinastri Renagupita, Rahadianto Rahadianto, Efyluk Garianto, Herin Setianingsih
Makara Journal of Science
This study aims to manifest the effect of the active compound phlorotannins in brown algae on decreasing insulin resistance by analyzing the predicted interaction between phlorotannins and protein tyrosine phosphatase 1B (PTP 1B) and estimating the pharmacokinetics and toxicity of the active compound for type 2 diabetes mellitus (DM) therapy. This type of research uses an in silico study to test the effect of using phlorotannins as an active compound in brown algae against PTP 1B inhibition. Starting from preparing materials, i.e., downloading the three-dimensional structure of phlorotannins via PubChem and PTP 1B via RSCB PDB (PDB 1A5Y), molecular docking …
Pva-Sansevieria Trifasciata Fraction Films As Absorbers Of Electromagnetic And Thermal Radiation On Smartphones, Nabiila Rahmani, Gustini Syahbirin, Akhiruddin Maddu, Purwantiningsih Sugita, Auliya Ilmiawati
Pva-Sansevieria Trifasciata Fraction Films As Absorbers Of Electromagnetic And Thermal Radiation On Smartphones, Nabiila Rahmani, Gustini Syahbirin, Akhiruddin Maddu, Purwantiningsih Sugita, Auliya Ilmiawati
Makara Journal of Science
Technological developments have prompted the production of highly advanced smartphones. However, various advanced features cause smartphones to heat up quickly and emit more electromagnetic radiation, which harms human health. Smartphone protection is needed to solve these issues. This study aims to create a film from the S. trifasciata plant based on polyvinyl alcohol (PVA) to reduce electromagnetic and thermal radiation on smartphones. S. trifasciata plants were macerated with acetone and partitioned with n-hexane, dichloromethane, ethyl acetate, and water. The films were made by mixing PVA with the four fractions. Among the four layers of film, the PVA + water …
Prevalence And Plasmid Profiles Of Enterobacteriaceae Isolates Obtained From Individuals With Asymptomatic Bacteriuria In Okada, Edo State, Nigeria, Osarumwense Precious Otote, Maureen Uchechukwu Okwu, Osazee Ekundayo Izevbuwa, Happy Ovuerhi Oferiofe
Prevalence And Plasmid Profiles Of Enterobacteriaceae Isolates Obtained From Individuals With Asymptomatic Bacteriuria In Okada, Edo State, Nigeria, Osarumwense Precious Otote, Maureen Uchechukwu Okwu, Osazee Ekundayo Izevbuwa, Happy Ovuerhi Oferiofe
Makara Journal of Science
Asymptomatic bacteriuria (ASB) is the presence of bacteria in apparently healthy individuals. Although, the prevalence varies widely with age, gender, and the presence or absence of genitourinary abnormalities, the detection of pathogenic bacteria in urine constitutes firm evidence of infection. The objectives of this study were to isolate members of the family Enterobacteriaceae from urine samples of research participants, and to determine the antibacterial susceptibility and plasmid profiles of the isolates. A total of 122 mid-stream urine samples consisting of 72 male and 50 female participants from selected primary and secondary schools were collected. The age of the participants used …