Life Sciences Commons^™

Diet Modification Reduces Pain And Improves Function In Adults With Osteoarthritis: A Systematic Review, Karen Stanfar, Corey Hawes, Mina Ghajar, Laura Byham-Gray, Diane R. Radler

Health & Biomedical Sciences Faculty Publications and Presentations

Background

The effect of dietary modifications on pain and joint function in adults with osteoarthritis (OA) is an emerging area of study. This systematic review aimed to evaluate if adults with OA who consume diets with a higher proportion of plant phenols and omega-3 fatty acids would have less pain and improved joint function than those with a higher proportion of saturated fatty acids, omega-6 fatty acids and refined carbohydrates.

Methods

Database searches of CINAHL (EBSCO), Clinical Trials (NIH-NLM), Cochrane Library (Wiley), Dissertation & Thesis Global (ProQuest), Embase (Elsevier), Medline (OVID), PubMed (NLM), Scopus (Elsevier), Web of Sciences (Clarivate) for …

Extracts Of Tamarillo, Horned Melon, And Raspberry, But Not Extract Of Pear, Inhibit Human Blood Platelet Aggregation: Investigating The Underlying Factors For Their Differential Mechanisms, Agnese Barin, Ranjit K. Das, Nasser E. Bastani, Per Ole Iversen, Asim K. Duttaroy

Health & Biomedical Sciences Faculty Publications and Presentations

Highlights

• Sugar-free extracts of tamarillo, horned melon (kiwano), and raspberry inhibited ADP-induced platelet aggregation in platelet-rich plasma.

• Analysis of untargeted metabolites revealed the presence of platelet inhibitory components such as benzoic acid, caffeic acid, and gallic acid in the sugar-free extracts of tamarillo, raspberry, and kiwano but not in pear extract.

• Extract of pear had no platelet anti-aggregatory effect.

• Tamarillo, horned melon (kiwano), and raspberry reduced the synthesis of TxB2 and the release of platelet factor 4.

Abstract

In addition to their roles in thrombosis, hyperactive platelets contribute to atherosclerosis. Since anti-platelet drugs are not recommended for …

Bioactive Food Components And Their Inhibitory Actions In Multiple Platelet Pathways, Diptimayee Das, Shubhamay Adhikary, Ranjit Kumar Das, Antara Banerjee, Arun Kumar Radhakrishnan, Sujay Paul, Surajit Pathak, Asim K. Duttaroy

Health & Biomedical Sciences Faculty Publications and Presentations

In addition to hemostasis and thrombosis, blood platelets are involved in various processes such as inflammation, infection, immunobiology, cancer metastasis, wound repair and angiogenesis. Platelets' hemostatic and non-hemostatic functions are mediated by the expression of various membrane receptors and the release of proteins, ions and other mediators. Therefore, specific activities of platelets responsible for the non-hemostatic disease are to be inhibited while leaving the platelet's hemostatic function unaffected. Platelets' anti-aggregatory property has been used as a primary criterion for antiplatelet drugs/bioactives; however, their non-hemostatic activities are not well known. This review describes the hemostatic and non-hemostatic function of human blood …

Evaluation Of The Equivalence Of Different Intakes Of Fruitflow In Affecting Platelet Aggregation And Thrombin Generation Capacity In A Randomized, Double-Blinded Pilot Study In Male Subjects, Ranjit K. Das, Tanushree Datta, Dipankar Biswas, Ruedi Duss, Niamh O’Kennedy, Asim K. Duttaroy

Health & Biomedical Sciences Faculty Publications and Presentations

Background: The water-soluble tomato extract, Fruitflow® is a dietary antiplatelet which can be used to lower platelet aggregability in primary preventative settings. We carried out a pilot study to investigate the range of intakes linked to efficacy and to make an initial assessment of variability in response to Fruitflow®.

Methods: Platelet response to adenosine diphosphate (ADP) agonist and thrombin generation capacity were monitored at baseline and 24 h after consuming 0, 30, 75, 150 or 300 mg of Fruitflow® in a randomized, double-blinded crossover study in male subjects 30-65 years of age (N = 12). Results were evaluated for equivalence …

Nuclear And Chloroplast Dnas Reveal Diverse Origins And Mis-Identifications Of Juniperus Cultivars From Windsor Gardens, Uk, Part 3 Of 3, Robert P. Adams, Sam T. Johnson, John Anderson, Keith Rushforth, Nicolas Valentin, Andrea E. Schwarzbach, Sonja Siljak-Yakovlev

Health & Biomedical Sciences Faculty Publications and Presentations

Ploidy was determined for 15 plants labeled as Juniperus squamata at the Windsor Gardens, UK and revealed 12 were tetraploids (2n=4x=44) and 3 were diploids (2n=2x=22). nrDNA (ITS) and cp DNA sequencing the tetraploids found: 4 J. squamata (4x); 4 J. tibetica (4x) x J. squamata (4x); 2 J. sabina var. balkanensis (4x) x J. squamata (4x); and one J. chinensis var. sargentii (4x) x J. squamata (4x). Sequencing the 3 diploids revealed: 2 J. pingii (2x) x J. pingii (2x); and 1 J. pingii (2x)? x J. komarovii(2x)? Ploidy analyses of 18 additional cultivars, putatively from Juniperus davurica, J. …

Long Distance Gene Flow Facilitated By Bird-Dispersed Seeds In Wind-Pollinated Species: A Story Of Hybridization And Introgression Between Juniperus Ashei And J. Ovata Told By Nrdna And Cpdna, Robert P. Adams, Sam T. Johnson, Andrea E. Schwarzbach

Health & Biomedical Sciences Faculty Publications and Presentations

nrDNA and cpDNA were sequenced of J. ashei and J. ovata from populations throughout their ranges. No J. ashei populations were found to be pure in their nrDNA for every tree, however all J. ashei trees in every population contained only the pure J. ashei chloroplast type. Populations of J. ovata in trans-Pecos Texas were almost pure in both nrDNA and cp DNA. Several plants in the J. ashei range contained J. ovata-type nrDNA (White Cliffs, AR, 3/10); Ranger, TX (1/5); Waco, TX (1/12). Every J ashei population contained at least 1 plant with hybrid (heterozygous) nrDNA and 3 J. …

The Increase Of Hiv-1 Infection, Neurocognitive Impairment, And Type 2 Diabetes In The Rio Grande Valley, Roberto De La Garza, Hansapani Rodrigo, Francisco Fernandez, Upal Roy

Health & Biomedical Sciences Faculty Publications and Presentations

The Human Immunodeficiency Virus (HIV-1) infection remains a persistent predicament for the State of Texas, ranking seventh among the most documented HIV cases in the United States. In this regard, the Rio Grande Valley (RGV) in South Texas is considered as one of the least investigated areas of the state with respect to HIV infection and HIV associated comorbidities. Considering the 115% increase in average HIV incidence rates per 100,000 within the RGV from 2007-2015, it is worth characterizing this population with respect to their HIV-1 infection, HIV-1 Associated Neurocognitive Disorders (HAND), and the association of treatment with combined antiretroviral …

Inheritance Of Single Copy Nuclear Genes (Scngs) In Artificial Hybrids Of Hesperocyparis Arizonica X H. Macrocarpa: Potential For Utilization In The Detection Of Hybridization In Natural Populations, Robert P. Adams, Sam T. Johnson, Mark Miller, Charles Low, Andrea E. Schwarzbach

Health & Biomedical Sciences Faculty Publications and Presentations

Analyses were performed on 18 artificial hybrids from a cross of Hesperocyparis arizonica (male parent) x H. macrocarpa (female parent) using 9 single copy nuclear genes (SCNGs). Three SCNG were found to be informative: myb, 4CL and CnAIB2. Gene myb contained 5 variable sites, of which site 89 was homozygous (CC, TT) as was site 261 (GG, AA) and useful for the detection of hybridization. All 18 hybrids were heterozygous (CT and GA) at these 2 sites as predicted in hybrids. 4CL contained 8 variable sites, of which 1 site (591) was homozygous (TT, CC) and all 18 hybrids were …

Discovery Of Juniperus Sabina Var. Balkanensis R. P. Adams And A. N. Tashev In Macedonia, Bosnia-Herzegovina, Croatia And Central And Southern Italy And Relictual Polymorphisms Found In Nrdna, Robert P. Adams, Adam Boratynski, Katarzyna Marcysiak, Francesco Roma-Marzio, Lorenzo Peruzzi, Fabrizio Bartolucci, Fabio Conti, Tuğrul Mataraci, Andrea E. Schwarzbach, Alexander N. Tashev, Sonja Siljak-Yakovlev

Health & Biomedical Sciences Faculty Publications and Presentations

Additional analyses of trnS-trnG and nrDNA from specimens from Bosnia-Herzegovina, southern and central, Italy, Croatia and Macedonia revealed the presence of J. sabina var. balkanensis in these areas west of the previously known populations in Greece, Bulgaria and western Turkey. Careful chromatogram analysis of eight (8) polymorphic sites in nrDNA revealed that nearly all of the populations of both var. balkanensis and var. sabina contained from 2 to 8 polymorphic sites. For these 8 heterozygous sites, two exclusive patterns were found in J. sabina. One type (GGACCCAG) was found in 16/62 plants and type 2 (ACGACAGT) was found in 4/62 …

The Juniperus Flaccida-J. Poblana Complex Revisited: Insights From Molecular And Oil Analysis, Robert P. Adams, M. Socorro González-Elizondo, Martha González-Elizondo, Andrea E. Schwarzbach

Health & Biomedical Sciences Faculty Publications and Presentations

Analysis of nrDNA, petN-psbM, trnS-trnG, trnD-trnT, and trnF-trnL revealed that J. flaccida is diverse, but quite separated from J. poblana and J. aff. poblana from Nayarit by at least 10 Mutational events (MEs, SNPs + indels), and is actually a bit closer to J. standleyi (8 MEs). A divergent J. poblana from Oaxaca is merely 2 MEs distant from the Nayarit trees. The J. poblana samples from the type locality (Amozoc, Pue.) vary by only 1 ME among samples. Leaf oils, on the other hand, show to the Nayarit juniper quite differentiated from other J. poblana. In addition, the habit …

Geographic Variation In Juniperus Drupacea: Dna Sequencing And Volatile Leaf Oils: Further Evidence Of Putative Pleistocene Genetic Isolation Between Europe And Asia, Robert P. Adams, Adam Boratynski, Magda Dou Dagher-Kharrat, Hagar V. Leschner, Tuğrul Mataraci, Andrea E. Schwarzbach

Health & Biomedical Sciences Faculty Publications and Presentations

Recently, Sobierajska et al. (2016), using nSSR and morphology, showed that Juniperus drupacea exhibited differentiation between Greece and Turkey/ Lebanon, suggestive of Pleistocene genetic isolation. Here, we report that leaf terpenoids and DNA sequence data support their hypothesis by confirming differentiation between Greece and Turkey/ Lebanon/ Israel. The leaf oils of the Turkey/ Lebanon plants contained one unique terpene (trans-verbenol, 0.1-1.4%) that was absent in the Greece plants. The Greece oil contained three terpenes not found in the Lebanon/ Turkey plants: (ar)-curcumene (2.2%), β-alaskene (0.3%) and α-alaskene (0.4%). Four other terpenes were in higher concentration in the Greece oils: camphene …

Principal Component Regression Analysis Of Nutrition Factors And Physical Activities With Diabetes, Ke-Sheng Wang, Ying Lu, Xin Xie, Shaoqing Gong, Chun Xu, Zhanxin Sha

Health & Biomedical Sciences Faculty Publications and Presentations

The associations of nutrition factors and physical activities with adult diabetes are inconsistent; while most of these factors are inter correlated. The aims of this study are to overcome the disturbance of the multicollinearity of the risk factors and examine the associations of these factors with diabetes using the principal component analysis (PCA) and regression analysis with principal component scores (PCS). Totally, 659 adults with diabetes and 2827 non-diabetic were selected from the 2012 Health Information National Trends Survey (HINTS 4, Cycle 2). PCA was utilized to deal with multicollinearity of the risk factors. Weighted univariate and multiple logistic regression …

Fungi Isolated From House Flies (Diptera: Muscidae) On Penned Cattle In South Texas, Cherity A. Ysquierdo, Pia U. Olafson, Donald B. Thomas

Health & Biomedical Sciences Faculty Publications and Presentations

Musca domestica L. were collected from cattle diagnosed with bovine ringworm to evaluate the potential of the house fly to disseminate Trichophyton verrucosum E. Bodin, a fungal dermatophyte that is the causative agent for ringworm in cattle. Fungal isolates were cultured from 45 individual flies on supplemented Sabouraud dextrose agar, and isolates were identified using morphological and microscopic approaches. Each isolate was identified further by PCR amplification of the ribosomal DNA locus with fungal-specific primers and subsequent amplicon sequencing. Trichophyton verrucosum was not identified using these approaches. However, 35 different fungal species representing 17 genera were cultured from collected flies, …

Discovery Of Juniperus Sabina Var. Balkanensis R. P. Adams And A. N. Tashev In Western Turkey (Anatolia), Robert P. Adams, Adam Boratynski, Tuğrul Mataraci, Alexander N. Tashev, Andrea E. Schwarzbach

Health & Biomedical Sciences Faculty Publications and Presentations

Additional analyses of trnS-trnG and nrDNA from herbarium specimens from Europe revealed the presence of J. sabina var. balkanensis in western Turkey near Izmir and expands the range previously known only from Bulgaria and adjacent mountains in Greece. A more detailed map of the taxon's distribution is presented.

Dna Sequencing And Taxonomy Of Unusual Serrate Juniperus From Mexico: Chloroplast Capture And Incomplete Lineage Sorting In J. Coahuilensis And Allied Taxa, Robert P. Adams, M. Socorro Gonzalez-Elizondo, Martha Gonzalez-Elizondo, David Ramirez Noya, Andrea E. Schwarzbach

Health & Biomedical Sciences Faculty Publications and Presentations

Analysis of nrDNA, petN-psbM, trnS-trnG, trmD-trnT, and trnF-trnL of Juniperus coahuilensis and allied taxa of Mexico found typical J. coahuilensis, as well as individuals with: coahuilensis cp and hybrid ITS; coahuilensis cp and novel ITS sequence (La Parrilla type); novel Blue Fruited cp (blue fruited taxon) and coahuilensis ITS; plus Blue Fruited cp and La Parrila ITS. nrDNA data was examined and found to detect hybridization, chloroplast capture and incomplete lineage sorting. In addition, a new taxon was found with Blue Fruited (Blue Fruited) cp and J. martinezii ITS, suggestive of chloroplast capture. New records of J. saltillensis were confirmed …

Chloroplast Capture In Juniperus Sabina Var. Balkanensis R. P. Adams And A. N. Tashev, From The Balkan Peninsula: A New Variety With A History Of Hybridization With J. Thurifera, Robert P. Adams, Andrea E. Schwarzbach, Alexander N. Tashev

Health & Biomedical Sciences Faculty Publications and Presentations

example of chloroplast capture has been identified in Juniperus sabina from Bulgaria and Greece in the Balkan peninsula. Nuclear DNA and overall morphology clearly indicate a close relationship to Juniperus sabina, whereas the cpDNA from these populations is very uniform and is nearly identical to that of J. thurifera, an unrelated species currently growing in France, Spain and Morocco. The new taxon is recognized as Juniperus sabina var. balkanensis R. P. Adams and A. Tashev. At present, this new variety is known only from locations in Bulgaria and Greece.

Evidence Of Relictual Introgression Or Incomplete Lineage Sorting In Nrdna Of Juniperus Excelsa And J. Polycarpos In Asia Minor, Robert P. Adams, Metin Armagan, Adam Boratynski, Bouchra Douaihy, Magda Dou Dagher-Kharrat, Vahid Farzaliyev, Salih Gucel, Tuğrul Mataraci, Andrea E. Schwarzbach

Health & Biomedical Sciences Faculty Publications and Presentations

DNA analysis of Juniperus excelsa from throughout its range revealed that J. polycarpos, instead of J. excelsa occupies central and eastern Turkey. Based on nrDNA (ITS) data, it appears that relictual hybridization has occurred in southeastern Turkey between J. polycarpos and J. turcomanica. Surprisingly, evidence of incomplete lineage sorting or relictual hybridization between J. polycarpos and J. seravschanica was found in central Turkey and northwest Iran.

Chloroplast Capture By A New Variety, Juniperus Sabina Var. Balkanensis R. P. Adams And A. N. Tashev, From The Balkan Peninsula: A Putative Stabilized Relictual Hybrid Between J. Sabina And Ancestral J. Thurifera, Robert P. Adams, Andrea E. Schwarzbach, Alexander N. Tashev

Health & Biomedical Sciences Faculty Publications and Presentations

An example of chloroplast capture has been found in Juniperus sabina from Bulgaria and Greece in the Balkan peninsula. The cpDNA from these populations is very uniform and is nearly identical to that of J. thurifera (currently growing in France, Spain and Morocco). The new taxon is recognized as Juniperus sabina var. balkanensis R. P. Adams and A. Tashev. At present, the variety, with the thurifera cpDNA, is known only from Bulgaria and Greece.

Geographic Variation In Nrdna And Four Cpdna Regions Of Juniperus Excelsa: Analysis Of New Records From Bulgaria, Cyprus And Southwestern Turkey, Robert P. Adams, Salih Gucel, Tuğrul Mataraci, Alexander N. Tashev, Bouchra Douaihy, Magda Dou Dagher-Kharrat, Vahid Farzaliyev, Andrea E. Schwarzbach

Health & Biomedical Sciences Faculty Publications and Presentations

Sequencing of nrDNA, plus four cp DNA regions: petN-psbM, trnS-trnG, trnD-trnT and trnL-trnF of newly acquired samples of J. excelsa from Bulgaria, Cyprus and Turkey showed little variation in J. excelsa (sensu stricto), except for the unusual situation in Lebanon, where J. excelsa and J. polycarpos (and likely J. p. var. turcomanica) grow near each other and may be hybridizing. The genetic composition of the eastern-most populations of J. excelsa in Turkey is unknown and deserves further study.

Contrasting Demographic History And Gene Flow Patterns Of Two Mangrove Species On Either Side Of The Central American Isthmus, Ivania Cerón-Souza, Elena G. Gonzalez, Andrea E. Schwarzbach, Dayana E. Salas-Leiva, Elsie Rivera-Ocasio, Nelson Toro-Perea, Eldredge Bermingham, W Owen Mcmillan

Health & Biomedical Sciences Faculty Publications and Presentations

Comparative phylogeography offers a unique opportunity to understand the interplay between past environmental events and life-history traits on diversification of unrelated but co-distributed species. Here, we examined the effects of the quaternary climate fluctuations and palaeomarine currents and present-day marine currents on the extant patterns of genetic diversity in the two most conspicuous mangrove species of the Neotropics. The black (Avicennia germinans, Avicenniaceae) and the red (Rhizophora mangle, Rhizophoraceae) mangroves have similar geographic ranges but are very distantly related and show striking differences on their life-history traits. We sampled 18 Atlantic and 26 Pacific locations for A. germinans (N = …

A New, Flaccid, Decurrent Leaf Variety Of Juniperus Poblana From Mexico: J. Poblana Var. Decurrens R. P. Adams, Robert P. Adams, Andrea E. Schwarzbach

Health & Biomedical Sciences Faculty Publications and Presentations

Analyses of nrDNA and four cp DNAs (petN-psbM, trnS-trnG, trnD-trnT, trnL-trnF) plus morphology and leaf essential oils revealed that the weeping (flaccid), decurrent leafed junipers near Topia, Durango are closely related to J. poblana (formerly J. flaccida var. poblana) and should be recognized as a new variety, J. poblana var. decurrens R. P. Adams var. nov. The leaf oil of J. p. var. decurrens is dominated by α-pinene (53.2%) with moderate amounts of β-pinene (5.3%), myrcene (4.3%), δ-2-carene (1.2%), δ-3-carene (2.5%), limonene (3.2%), β-phellandrene (3.1%), terpinolene (1.0%), (E)-caryphyllene (1.1%), and germacrene D (1.5%) and shares eleven unique terpenes with J. …

Juniperus Communis In Morocco: Analyses Of Nrdna And Cpdna Regions, Robert P. Adams, Mustapha Rhanem, Andrea E. Schwarzbach

Health & Biomedical Sciences Faculty Publications and Presentations

Juniperus communis from Morocco was analyzed by DNA sequence data from nrDNA plus four cp DNA regions (4315 bp) and found to be in a clade with other J. communis from Europe. The sub-alpine, prostrate J. communis plants of Morocco, each only contained a single SNP and a one bp indel (deletion) in the trnSG sequence data for a total of 2 MEs (mutation events). The Moroccan prostrate J. communis appear to be a variant of J. communis of Europe and central Asia and not part of the var. hemispherica group.

Juniperus Communis In Azerbaijan: Analyses Of Nrdna And Cpdna Regions, Robert P. Adams, Vahid Farzaliyev, Alexander N. Tashev, Andrea E. Schwarzbach

Health & Biomedical Sciences Faculty Publications and Presentations

Juniperus 'pygmaea' from Azerbaijan was analyzed by DNA sequence data from nrDNA plus four cp DNA regions (4315 bp) and found in a clade with J. communis 'oblonga' (= J. communis) Armenia, not with J. c. forma pygmaea of Bulgaria. It seems prudent to not recognize this variant taxonomically but treat it as J. communis.

Associations Between Pqct-Based Fat And Muscle Area And Density And Dxa-Based Total And Leg Soft Tissue Mass In Healthy Women And Men, V. D. Sherk, R. S. Thiebaud, Z. Chen, Murat Karabulut, S. J. Kim, D. A. Bemben

Health & Biomedical Sciences Faculty Publications and Presentations

Peripheral Quantitative Computed Tomography (pQCT) can be used for muscle and fat area and density assessments. These may independently influence muscle and fat mass measurements from Dual Energy X-ray Absorptiometry (DXA).

Objective

To determine associations between pQCT-derived soft tissue density and area measures and DXA-derived soft tissue mass.

Methods

Linear regression models were developed based on BMI and calf fat and muscle cross-sectional area (FCSA and MCSA) and density measured by pQCT in healthy women (n=76) and men (n=82) aged 20–59 years. Independent variables for these models were leg and total bone-free lean mass (BFLM) and fat mass (FM) measured …

Predominant And Substoichiometric Isomers Of The Plastid Genome Coexist Within Juniperus Plants And Have Shifted Multiple Times During Cupressophyte Evolution, Wenhu Guo, Felix Grewe, Amie Cobo-Clark, Weishu Fan, Zelin Duan, Robert P. Adams, Andrea E. Schwarzbach, Jeffrey P. Mower

Health & Biomedical Sciences Faculty Publications and Presentations

Most land plant plastomes contain two copies of a large inverted repeat (IR) that promote high-frequency homologous recombination to generate isomeric genomic forms. Among conifer plastomes, this canonical IR is highly reduced in Pinaceae and completely lost from cupressophytes. However, both lineages have acquired short, novel IRs, some of which also exhibit recombinational activity to generate genomic structural diversity. This diversity has been shown to exist between, and occasionally within, cupressophyte species, but it is not known whether multiple genomic forms coexist within individual plants. To examine the recombinational potential of the novel cupressophyte IRs within individuals and between species, …

Tuning Of Alternative Splicing - Switch From Proto-Oncogene To Tumor Suppressor, Aleksandra Shchelkunova, Boris Ermolinsky, Meghan Boyle, Ivan Mendez, Michael Lehker, Karen S. Martirosyan, Alexander V. Kazanksy

Health & Biomedical Sciences Faculty Publications and Presentations

STAT5B, a specific member of the STAT family, is intimately associated with prostate tumor progression. While the full form of STAT5B is thought to promote tumor progression, a naturally occurring truncated isoform acts as a tumor suppressor. We previously demonstrated that truncated STAT5 is generated by insertion of an alternatively spliced exon and results in the introduction of an early termination codon. Present approaches targeting STAT proteins based on inhibition of functional domains of STAT's, such as DNA-binding, cooperative binding (protein-protein interaction), dimerization and phosphorylation will halt the action of the entire gene, both the proto-oncogenic and tumor suppressor functions …

Efectos Benéficos Del Aloe En La Salud, Mariel Calderón Oliver, Maria Alejandra Quinones Peña, José Pedraza Chaverri

Health & Biomedical Sciences Faculty Publications and Presentations

El Aloe es una planta originaria de regiones áridas que se ha empleado como remedio medicinal desde la antigüedad. Su uso en diferentes patologías depende de cuestiones biológicas, de la variedad de la planta e incluso de cuestiones culturales. Se conocen más de 360 especies de Aloe y se conocen estudios científicos sólo de algunas de las especies con propiedades medicinales, siendo el Aloe vera la especie más estudiada y comercializada en la actualidad. Ciertos compuestos del Aloe presentan propiedades benéficas en la salud, tal es el caso de algunos polifenoles y antracenos como el aloe-emodina, losacemananos, etc. Muchos de …