Life Sciences Commons^™

Assessment Of Factors Affecting Establishment Of Biological Control Agents Of Hemlock Woolly Adelgid On Eastern Hemlock In The Great Smoky Mountains National Park, Abdul Hakeem

Doctoral Dissertations

Eastern hemlock in the Great Smoky Mountains National Park (GRSM) is threatened by hemlock woolly adelgid (HWA), Adelges tsugae Annand (Hemiptera: Adelgidae). To manage this invasive pest in GRSM, ca. 550,000 Sasajiscymnus tsugae (Sasaji and McClure) (Coleoptera: Coccinellidae) and 7,857 Laricobius nigrinus Fender (Coleoptera: Derodontidae) have been released. Limited information is available on their establishment in GRSM. To fill this gap, a study was initiated to assess establishment of these predators and their impact on hemlock health. To determine factors affecting establishment of these predators, 65 release sites were sampled from 2008 to 2012. Several factors were evaluated for their …

Aasp-Certified Consultants’ Experiences Of Spirituality Within Sport Psychology Consultation, Trevor Jonas Egli

Doctoral Dissertations

According to Watson and Nesti (2005), the scholarly investigation of spirituality within sport psychology is lacking. Recently, within cultural sport psychology (Schinke & Hanrahan, 2009), a discussion of spirituality has been initiated; however, it has not received the same amount of attention as other aspects of culture that impact sport performance and mental well-being (Butryn, 2002, 2010; Duda & Allison, 1990; Kontos & Breland-Noble, 2002; Krane, Waldron, Kauer & Semerjian, 2010; McGannon & Busanich, 2010). Therefore, the purpose of this study was to understand how AASP-certified (CC-AASP) consultants understand spirituality. Nine AASP-certified (CC-AASP) consultants who have encountered spirituality in their …

Characterization Of Field Evolved Resistance To Transgenic Cry1fa Maize In Spodoptera Frugiperda (J. E. Smith), Siva Rama Krishna Jakka

Doctoral Dissertations

Transgenic Bt crops expressing Cry and Vip toxins from Bacillus thuringiensis (Bt) have been increasingly planted to manage insect pest damage on agricultural crops. The high adoption of Bt-based insecticidal technologies suggests an increase selection pressure for the evolution of resistance in insect populations. So far, nine insect species have developed field evolved resistance to Bt crops, yet the mechanisms involved in field evolved resistance are unknown. In the present study, the resistance mechanism in field evolved resistance to maize producing Cry1Fa in Spodoptera frugiperda collected in fields from Puerto Rico was characterized. High levels of resistance to …

Experimental And Computational Analysis Of Chloroplast Transit Peptide Domain Architecture And Function, Prakitchai Chotewutmontri

Doctoral Dissertations

The Majority of chloroplast proteins are nuclear-encoded and utilize an N-terminal transit peptide (TP) to target into chloroplasts via the general import pathway. Bioinformatic and proteomic analyses provide thousands of predicted TPs, which show low sequence similarity. How the common chloroplast translocon components recognize these diverse TPs is not well understood. Previous results support either sequence- or physicochemical-specific recognitions. To further address this question, a reverse sequence approach was utilized such that the reverse TP contains the same amino acid composition as wild-type TP but lack similar sequence motifs. Using both native and reverse TPs of the two well-studied precursors, …

Effects Of Leaflet Orientation And Root Morphology On Physiological Traits And Yield In Soybeans., Richard Dewayne Johnson

Doctoral Dissertations

Drought is the most important abiotic stress adversely affecting soybean (Glycine max (L.) Merr.) yield. Leaflet orientation has been shown to reduce leaflet temperatures and transpiration while root morphology has been related to slower wilting phenotypes. The objective of this study was to investigate effects of leaflet orientation and rooting morphology on whole plant transpiration, yield, water use efficiency, and other physiological traits in soybeans using grafting techniques, population lines, near-isogenic lines, and restrained leaf canopy experiments. Experiments were conducted in Knoxville, TN with additional yield trial plots at Springfield, Spring Hill, and Milan, TN. Data were collected on …

Soybean Nodulin 26: A Channel For Water And Ammonia At The Symbiotic Interface Of Legumes And Nitrogen-Fixing Rhizobia Bacteria, Jin Ha Hwang

Doctoral Dissertations

During the infection and nodulation of legume roots by soil bacteria of the Rhizobiaceae family, the invading endosymbiont becomes enclosed within a specialized nitrogen-fixing organelle known as the "symbiosome". In mature nodules the host infected cells are occupied by thousands of symbiosomes, which constitute the major organelle within this specialized cell type. The symbiosome membrane is the outer boundary of this organelle which controls the transport of metabolites between the symbiont and the plant host. These transport activities include the efflux of the primary metabolic product of nitrogen fixation and the uptake of dicarboxylates as an energy source to support …

Effects Of Foot Type On Multi-Segment Foot Motion In High- And Low-Arched Female Recreational Athletes, Douglas W. Powell

Doctoral Dissertations

Introduction: Mal-alignment and dysfunction of the foot have been shown to result in an increased rate of injury and unique injury patterns. Aberrant foot function has been shown to contribute to repetitive stress and acute injuries. High-arched athletes have been shown to experience a greater rate of bony injury to the lateral aspect of the lower extremity while low-arched athletes experience greater rates of soft-tissue injury to the medial aspect of the lower extremity. Though foot type has been linked to these injury patterns, the mechanism by which these injury patterns occur remains unknown. Multi-segment foot models have been developed …

Development And Application Of Mass Spectrometry-Based Proteomics To Generate And Navigate The Proteomes Of The Genus Populus, Paul Edward Abraham

Doctoral Dissertations

Historically, there has been tremendous synergy between biology and analytical technology, such that one drives the development of the other. Over the past two decades, their interrelatedness has catalyzed entirely new experimental approaches and unlocked new types of biological questions, as exemplified by the advancements of the field of mass spectrometry (MS)-based proteomics. MS-based proteomics, which provides a more complete measurement of all the proteins in a cell, has revolutionized a variety of scientific fields, ranging from characterizing proteins expressed by a microorganism to tracking cancer-related biomarkers. Though MS technology has advanced significantly, the analysis of complicated proteomes, such as …

Multiscale Modeling Of Enzyme-Catalyzed Methanol Production By Particulate Methane Monooxygenase, Katherine K. Bearden

Doctoral Dissertations

In this work, the conversion of methane to methanol by the particulate Methane Monooxygenase (pMMO) enzyme is investigated using a multi-scale modeling approach. This enzyme participates in carbon cycling and aids in the removal of harmful atmospheric methane, converting it to methanol. The interaction between pMMO and a neighboring enzyme that is present in the same organism is studied, and the unknown pMMO active site is elucidated and tested for methane oxidation towards the production of methanol.

Fundamental knowledge of pMMO's mechanism is not fully understood. Understanding how this enzyme works in nature will provide information towards designing efficient synthetic …

The Measurement Of Auditory Interhemispheric Transfer Time (Ihtt) In Children With Normal Auditory Processing Abilities, Brittany Suzanne Keahey

Doctoral Dissertations

Interhemispheric transfer time (IHTT) is the time it takes for information to be transmitted from one hemisphere to the other. The goal of this study was to determine if differences existed in the IHTT of children 6 to 9 years of age with normal auditory processing abilities by the use of an objective measure (auditory late evoked potentials [ALEPs]), specifically waves P1, N1 and P2. It was hypothesized that there would be no difference in IHTT between the groups due to the age range of participants being tested. The 16 participants were divided into two groups based on age and …

Engineering Microenvironments To Modulate Calcium Information Processing In Neuronal Cells, Kinsey Cotton Kelly

Doctoral Dissertations

Tissue engineered microenvironments were constructed to test the effects glial cells have on calcium information processing, and to mimic conditions in vivo for tumor invasion and residual cancer after resection of tumor. Submaximal, nM, glutamate (GLU) stimuli were applied to the engineered environments, and the resulting calcium dynamic behavior of neuronal cells was measured to help predict and interpret chaotic systems in the experimental realm. Calcium is a key signaling ion which signals through the N-methyl-D-aspartate (NMDA) glutamate receptor on the neuronal membrane. GLU binding to the NMDA receptor (NMDAR) causes a large and dynamic increase in neuronal intracellular calcium. …