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Full-Text Articles in Life Sciences

A Comparison Of The Growth And Asexual Reproduction By Cryphonectria Parasitica Isolates Infected With Hypoviruses Chv3-County Line, Chv1-Euro7, And Chv1-Ep713, Jenise Bauman Jan 2015

A Comparison Of The Growth And Asexual Reproduction By Cryphonectria Parasitica Isolates Infected With Hypoviruses Chv3-County Line, Chv1-Euro7, And Chv1-Ep713, Jenise Bauman

College of the Environment on the Peninsulas Publications

The impact of chestnut blight, caused by the fungal pathogen Cryphonectria parasitica, has diminished in Europe due to a natural biological control caused by hypovirus infection. Hypovirulence- mediated biological control has been far less successful in North America meriting further evaluation of field isolates that have the ability to produce non-lethal cankers, generate hypovirulent inoculum, and exhibit a greater ecological fitness in forest systems. In this study, Cryphonectria hypoviruses (CHV) CHV3-County Line, CHV1-Euro7, and CHV1-Ep713 were evaluated in five different isolates of C. parasitica. One hundred and eighty cankers representing each treatment combination were initiated on American chestnut sprouts in …


American Chestnut's Role In The Ecological Restoration Of Coal-Mined Landscapes, Jenise Bauman, Caleb Cochran, Brian C. Murphy, Carolyn H. Keiffer Jan 2013

American Chestnut's Role In The Ecological Restoration Of Coal-Mined Landscapes, Jenise Bauman, Caleb Cochran, Brian C. Murphy, Carolyn H. Keiffer

College of the Environment on the Peninsulas Publications

The American chestnut’s fast growth rate, early nut production, and quality of timber make it a valuable tree for use in coal mine restoration projects (Figure 1). This species tolerates a wide range of ecological conditions, including dry soils and low pH, which are typical of some sites previously mined for coal. Experimental planting methods are currently being studied to determine protocols most conducive for establishing chestnut trees on these sites. The Forestry Reclamation Approach (FRA) proposed by the Appalachian Regional Reforestation Initiative (ARRI) recommends the selection of proper soil substrate, a deep rooting zone, appropriate herbaceous vegetation, and the …


Survey For The Presence Of Phytophthora Cinnamomi On Reclaimed Mined Lands In Ohio Chosen For Restoration Of The American Chestnut, Shiv Hiremath, Kirsten J. Lehtoma, Jenise Bauman Jan 2013

Survey For The Presence Of Phytophthora Cinnamomi On Reclaimed Mined Lands In Ohio Chosen For Restoration Of The American Chestnut, Shiv Hiremath, Kirsten J. Lehtoma, Jenise Bauman

College of the Environment on the Peninsulas Publications

We have been planting blight resistant American chestnut seedlings on reclaimed coal mined areas in Southeastern Ohio, which was once within the natural range of the American chestnut. Towards the goal of restoring the American chestnut, we are testing suitable sites that can aid survival, growth and establishment of planted seedlings pre-inoculated with ectomycorrhizal fungi. Prior to the arrival of the chestnut blight fungus, pathogens of the genus Phytophthora were introduced in the USA that were responsible for the “ink disease” or “root-rot” resulting in wide-spread death of chestnut trees in southern states. Although these pathogens were not observed elsewhere, …


Ailanthus Altissima (Tree-Of-Heaven) Interferes With Beneficial Symbionts With Negative Impacts On Oak Regeneration, Jenise Bauman, Caitlin Byrne, Shiv Hiremath Jan 2013

Ailanthus Altissima (Tree-Of-Heaven) Interferes With Beneficial Symbionts With Negative Impacts On Oak Regeneration, Jenise Bauman, Caitlin Byrne, Shiv Hiremath

College of the Environment on the Peninsulas Publications

The invasion of Tree-of-Heaven (Ailanthus altissima) has been documented in disturbed landscapes leading to biodiversity loss and degradation of ecosystem function. Ailanthus interferes with the restoration of native species by its aggressive growth habit, alteration of nutrient cycles, and allelopathic chemical production. Recent studies suggest that allelopathy has a negative effect on the growth of red oak (Quercus rubra), possibly by interfering with the symbiosis of beneficial ectomycorrhizal fungi (ECM). This fungal symbiont is essential for healthy tree growth and the unavailability of these fungi may impede the success of seedling regeneration. This study investigated the effects …


Noninvasive Method For A Statewide Survey Of Eastern Hellbenders Cryptobranchus Alleganiensis Using Environmental Dna, Amy J. Santas, Tyler Persaud, Barbara A. Wolfe, Jenise Bauman Jan 2013

Noninvasive Method For A Statewide Survey Of Eastern Hellbenders Cryptobranchus Alleganiensis Using Environmental Dna, Amy J. Santas, Tyler Persaud, Barbara A. Wolfe, Jenise Bauman

College of the Environment on the Peninsulas Publications

Traditional survey methods of aquatic organisms may be difficult, lengthy, and destructive to the habitat. Some methods are invasive and can be harmful to the target species. The use of environmental DNA (eDNA) has proven to be effective at detecting low population density aquatic macroorganisms. This study refined the technique to support statewide surveys. Hellbender presence was identified by using hellbender specific primers (cytochrome b gene) to detect eDNA in water samples collected at rivers, streams and creeks in Ohio and Kentucky with historical accounts of the imperiled eastern hellbender (Cryptobranchus a. alleganiensis). Two sampling protocols are described; …


Sustainable Landscapes: Evaluating Strategies For Controlling Autumn Olive (Elaeagnus Umbellata) On Reclaimed Surface Mineland At The Wilds Conservation Center In Southeastern Ohio, Shana M. Byrd, Nicole D. Cavender, Corine M. Peugh, Jenise Bauman Jan 2012

Sustainable Landscapes: Evaluating Strategies For Controlling Autumn Olive (Elaeagnus Umbellata) On Reclaimed Surface Mineland At The Wilds Conservation Center In Southeastern Ohio, Shana M. Byrd, Nicole D. Cavender, Corine M. Peugh, Jenise Bauman

College of the Environment on the Peninsulas Publications

Autumn olive (Elaeagnus umbellata) was planted during the reclamation process to reduce erosion and improve nitrogen content of the soil. However, since its establishment, E. umbellata has spread prolifically and control measures are difficult. The primary objective of this case study was to evaluate the effectiveness of various control methods on eradication of E. umbellata in varying degrees of infestation. A two-phase case study was conducted at The Wilds conservation center in Cumberland, OH. Phase 1 began in 2007-2008 to evaluate three treatments in areas with moderate cover (15-30%) of E. umbellata: mechanical removal, foliar herbicide, and dormant stem …


The Efficiency Of Introduced Pisolithus Tinctorius On Backcrossed Chestnut Germination And Survival., Jenise Bauman, Carolyn H. Keiffer, Shiv Hiremath Jan 2012

The Efficiency Of Introduced Pisolithus Tinctorius On Backcrossed Chestnut Germination And Survival., Jenise Bauman, Carolyn H. Keiffer, Shiv Hiremath

College of the Environment on the Peninsulas Publications

American chestnut was eliminated as a canopy tree from the Appalachian region of North America with the introduction of chestnut blight in the early 1900s. Breeding programs initiated in the 1980s have produced seedling lines that display the pure American morphology with potential resistance to chestnut blight. More work is required to assess their field performance in field sites representative of their native range. This study used American (Castanea dentata) and backcrossed hybrid chestnuts (C. dentata × C. mollissima) on an abandoned coal mine in southeastern Ohio. Half of the seeds were planted with ectomycorrhizal fungus …


Facilitation Of American Chestnut (Castanea Dentata) Seedling Establishment By Pinus Virginiana In Mine Restoration, Jenise Bauman, Carolyn H. Keiffer, Shiv Hiremath Jan 2012

Facilitation Of American Chestnut (Castanea Dentata) Seedling Establishment By Pinus Virginiana In Mine Restoration, Jenise Bauman, Carolyn H. Keiffer, Shiv Hiremath

College of the Environment on the Peninsulas Publications

This study evaluated the influence of planting sites on the establishment and ectomycorrhizal (ECM) colonization of American chestnut (Castanea denetata (Marsh.) Borkh.) on an abandoned coal mine in an Appalachian region of the United States. Root morphotyping and sequencing of the fungal internal transcribed spacer (ITS) region were used to identify the ECM species associated with the chestnut seedlings. Germination, survival, ECM root colonization, and growth were assessed in three habitats: forest edge, center (plots without vegetation), and pine plots (a 10-year-old planting of Pinus virginiana). Seedlings in pine plots had higher survival (38%) than the other plot …


Reforesting Ohio Using A Historical Legend: Blight-Resistant Chestnut Hybrids As A Coal Mine Restoration Tree, Jenise Bauman Apr 2011

Reforesting Ohio Using A Historical Legend: Blight-Resistant Chestnut Hybrids As A Coal Mine Restoration Tree, Jenise Bauman

College of the Environment on the Peninsulas Publications

The American chestnut (Castanea dentata) once occupied twenty-five percent of the eastern forests in the United States. Its massive size and prolific nut production made this species ecologically and economically important. At maturity, this species could top over 100 feet with immense, wide-spreading canopies. As a nut crop, the American chestnut tree was plentiful and considered one of the most important protein sources for wildlife such as squirrels, wild turkeys, deer, black bears, and many others. The lumber produced was extremely rot resistant and used for the construction of cabins, …


The Influence Of Inoculated And Native Ectomycorrhizal Fungi On Morphology, Physiology And Survival Of American Chestnut, Jenise Bauman, Carolyn H. Keiffer, Shiv Hiremath Jan 2011

The Influence Of Inoculated And Native Ectomycorrhizal Fungi On Morphology, Physiology And Survival Of American Chestnut, Jenise Bauman, Carolyn H. Keiffer, Shiv Hiremath

College of the Environment on the Peninsulas Publications

The objective of this study was to evaluate the influence of five different species of ectomycorrhizal (ECM) fungi on root colonization of native fungi on putatively blight resistant chestnut hybrids (Castanea dentata x C. mollissima) in a reclaimed mine site in central Ohio. The five species were Hebeloma crustuliniforme, Laccaria bicolor, Scleroderma polyrhizum, Amanita rubescens, and Suillus luteus. We used a combination of DNA sequencing of the ITS region and phylogenetic analyses to indentify fungi found on roots after 12 and 18 months in the field. Non-metric multidimensional scaling (NMDS) ordinations were used to determine …


The World Beneath Our Feet: Mycorrhizal Networking And The Establishment Of Ohio’S Native Trees And Shrubs, Jenise Bauman Jan 2009

The World Beneath Our Feet: Mycorrhizal Networking And The Establishment Of Ohio’S Native Trees And Shrubs, Jenise Bauman

College of the Environment on the Peninsulas Publications

Fungi are not random occurrences, but have evolved over millions of years. The relationship between native fungi and Ohio's trees and shrubs roots deep and is much more than what appears on the surface. The mushroom is the above ground fungal body that produces and disperses the spores. That very structure is made of interwoven, tightly packed, filamentous hyphae.