Life Sciences Commons^™

The Hox Protein Conundrum: The “Specifics” Of Dna Binding For Hox Proteins And Their Partners, Bony De Kumar, Diane C. Darland

Biology Faculty Publications

Homeotic genes (Hox genes) are homeodomain-transcription factors involved in conferring segmental identity along the anterior-posterior body axis. Molecular characterization of HOX protein function raises some interesting questions regarding the source of the binding specificity of the HOX proteins. How do HOX proteins regulate common and unique target specificity across space and time? This review attempts to summarize and interpret findings in this area, largely focused on results from in vitro and in vivo studies in Drosophila and mouse systems. Recent studies related to HOX protein binding specificity compel us to reconsider some of our current models for transcription factor-DNA …

Pre-Existing Microfilarial Infections Of American Robins (Passeriformes: Turdidae) And Common Grackles (Passeriformes: Icteridae) Have Limited Impact On Enhancing Dissemination Of West Nile Virus In Culex Pipiens Mosquitoes (Diptera: Culicidae), Jefferson A. Vaughan, Juanita Hinson, Elizabeth S. Andrews, Michael J. Turrell

Biology Faculty Publications

Microfilariae (MF) are the immature stages of filarial nematode parasites and inhabit the blood and dermis of all classes of vertebrates, except fish. Concurrent ingestion of MF and arboviruses by mosquitoes can enhance mosquito transmission of virus compared to when virus is ingested alone. Shortly after being ingested, MF penetrate the mosquito’s midgut and may introduce virus into the mosquito’s hemocoel, creating a disseminated viral infection much sooner than normal. This phenomenon is known as microfilarial enhancement. Both American Robins and Common Grackles harbor MF—that is, Eufilaria sp. and Chandlerella quiscali von Linstow (Spirurida: Onchocercidae), respectively. We compared infection and …

Plasticity Of Least Tern And Piping Plover Nesting Behaviors In Response To Sand Temperature, Alicia K. Andes, Mark H. Sherfy, Terry L. Shaffer, Susan N. Ellis-Felege

Biology Faculty Publications

Birds that nest on the ground in open areas, such as Piping Plovers (Charadrius melodus) and Interior Least Terns (Sternula antillarum athalassos), are exposed to high temperatures in thermally stressful environments. As a result, some ground-nesting avian species have adapted behavioral strategies to maintain thermal regulation of eggs and themselves. We assessed the impact of sand temperature on shorebird nesting behaviors by installing video cameras and thermocouples at 52 Least Tern and 55 Piping Plover nests on the Missouri River in North Dakota during the 2014–2015 breeding seasons. Daily duration and frequency of shading behaviors exhibited …

Deep Multilayer Brain Proteomics Identifies Molecular Networks In Alzheimer's Disease Progression, Bing Bai, Xusheng Wang, Yuxin Li, Ping-Chung Chen, Kaiwen Yu, Kaushik Kumar Dey, Jay M. Yarbro, Xian Han, Brianna M. Lutz, Shuquan Rao, Yun Jiao, Jeffrey M. Sifford, Jonghee Han, Minghui Wang, Haiyan Tan, Timothy I. Shaw, Ji-Hoon Cho, Suiping Zhou, Hong Wang, Mingming Niu, Ariana Mancieri, Kaitlynn A. Messler, Xiaojun Sun, Zhiping Wu, Vishwajeeth Pagala, Anthony A. High, Wenjian Bi, Hui Zhang, Hongbo Chi, Vahram Haroutunian, Bin Zhang, Thomas G. Beach, Gang Yu, Junmin Peng

Biology Faculty Publications

Alzheimer's disease (AD) displays a long asymptomatic stage before dementia. We characterize AD stage-associated molecular networks by profiling 14,513 proteins and 34,173 phosphosites in the human brain with mass spectrometry, highlighting 173 protein changes in 17 pathways. The altered proteins are validated in two independent cohorts, showing partial RNA dependency. Comparisons of brain tissue and cerebrospinal fluid proteomes reveal biomarker candidates. Combining with 5xFAD mouse analysis, we determine 15 Aβ-correlated proteins (e.g., MDK, NTN1, SMOC1, SLIT2, and HTRA1). 5xFAD shows a proteomic signature similar to symptomatic AD but exhibits activation of autophagy and interferon response and lacks human-specific deleterious events, …

Helianthus Maximiliani And Species Fine-Scale Spatial Pattern Affect Diversity Interactions In Reconstructed Tallgrass Prairies, Thomas P. Mckenna, Jack Mcdonnell, Kathryn A. Yurkonis, Caroline Brophy

Biology Faculty Publications

1. Biodiversity and Ecosystem Function analyses aim to explain how individual spe‐ cies and their interactions affect ecosystem function. With this study, we asked in what ways do species interact, are these interactions affected by species planting pattern, and are initial (planted) proportions or previous year (realized) propor‐ tions a better reference point for characterizing grassland diversity effects?

2. We addressed these questions with experimental communities compiled from a pool of 16 tallgrass prairie species. We planted all species in monocultures and mixtures that varied in their species richness, evenness, and spatial pattern. We recorded species‐specific biomass production over three …

Red Oak Seedlings As Indicators Of Deer Browse Pressure: Gauging The Outcome Of Different White-Tailed Deer Management Approaches, Bernd Blossey, Paul Curtis, Jason Boulanger, Andrea Davalos

Biology Faculty Publications

After decades of high deer populations, North American forests have lost much of their previous biodiversity. Any landscape‐level recovery requires substantial reduc‐ tions in deer herds, but modern societies and wildlife management agencies appear unable to devise appropriate solutions to this chronic ecological and human health crisis. We evaluated the effectiveness of fertility control and hunting in reducing deer impacts at Cornell University. We estimated spring deer populations and planted Quercus rubra seedlings to assess deer browse pressure, rodent attack, and other factors compromising seedling performance. Oak seedlings protected in cages grew well, but deer annually browsed ≥60% of unprotected …

Measuring Bird Damage To Three Fruit Crops: A Comparison Of Grower And Field Estimates, Julie L. Elser, Catherine A. Lindell, Karen M. M. Steensma, Paul D. Curtis, Deanna K. Leigh, William F. Siemer, Jason Boulanger, Stephanie A. Shwiff

Biology Faculty Publications

Birds are common pests in fruit orchards. They frequently consume and damage fruit resulting in decreased yields for growers. The true extent of damage is difficult to measure. Producer surveys are often implemented to estimate damage, but the accuracy of these estimates is uncertain. We compared damage estimates obtained through field studies with estimates from a producer survey for three fruit crops: wine grapes, sweet cherries, and ‘Honeycrisp’ apples. We also analyzed relationships between use of various damage management methods and levels of bird damage. We found wine grape and sweet cherry growers accurately assessed bird damage, while ‘Honeycrisp’ apple …

Fipronil And Ivermectin Treatment Of Cattle Reduced The Survival And Ovarian Development Of Field-Collection Anopheles Albimanus In A Pilot Trial Conducted In Northern Belize, Staci M. Dreyer, Donovan Leiva, Marla Magana, Marie Pott, Jonathan Kay, Alvaro Cruz, Nicole L. Achee, John P. Grieco, Jefferson A. Vaughan

Biology Faculty Publications

Background: Most malaria vector control programmes rely on indoor residual spraying of insecticides and insecticide-treated bed nets. This is efective against vector species that feed indoors at night and rest inside the house afterwards. In Central America, malaria vectors have diferent behaviours and are typically exophagic (i.e., bite outdoors), exophilic (i.e., remain outdoors after feeding), and zoophagic (i.e., as likely to feed on non-humans as humans). Thus, malaria elimination in Central America may require additional tactics. This pilot study investigated whether commercially-available products used to treat livestock for ticks could also be used to kill and/or sterilize zoophagic malaria vectors …

A Comparison Of Drone Imagery And Groundbased Methods For Estimating The Extent Of Habitat Destruction By Lesser Snow Geese (Anser Caerulescens Caerulescens) In La Pérouse Bay, Andrew F. Barnas, Brian J. Darby, Gregory S. Vandeberg, Robert F. Rockwell, Susan N. Ellis-Felege

Biology Faculty Publications

Lesser snow goose (Anser caerulescens caerulescens) populations have dramatically altered vegetation communities through increased foraging pressure. In remote regions, regular habitat assessments are logistically challenging and time consuming. Drones are increasingly being used by ecologists to conduct habitat assessments, but reliance on georeferenced data as ground truth may not always be feasible. We estimated goose habitat degradation using photointerpretation of drone imagery and compared estimates to those made with ground-based linear transects. In July 2016, we surveyed five study plots in La Pérouse Bay, Manitoba, to evaluate the effectiveness of a fixed-wing drone with simple Red Green Blue …

Molecular Phylogeny Provides New Insights On The Taxonomy And Composition Of Lyperosomum Looss, 1899 (Digenea, Dicrocoeliidae) And Related Genera, Joanna Hildebrand, Ewa Pyrka, Jiljí Sitko, Witold Jeżewski, Grzegorz Zaleśny, Vasyl V. Tkach, Zdzisław Laskowski

Biology Faculty Publications

Lyperosomum Looss, 1899 is one of the largest genera of the Dicrocoeliidae and is one of the best examples of the systematic complexity and taxonomic instability within this family. We present the molecular analyses based on novel sequences of nuclear and mitochondrial genes obtained from 56 isolates of adult flukes and larval stages of dicrocoeliids belonging to Lyperosomum, Skrjabinus, Zonorchis as well as previously available sequence data. According to obtained results we propose to return Zonorchis clathratus and Z. petiolatus into Lyperosomum, and to recognize L. alagesi as a synonym of L. petiolatum. Our study shows that L. petiolatum commonly …

Schlafen 3 Knockout Mice Display Gender-Specific Differences In Weight Gain, Food Efficiency, And Expression Of Markers Of Intestinal Epithelial Differentiation, Metabolism, And Immune Cell Function, Emilie E. Vomhof-Dekrey, Jun Lee, Jack Lansing, Chris Brown, Diane Darland, Marc D. Basson

Biology Faculty Publications

Self-renewal and differentiation are essential for intestinal epithelium absorptive functioning and adaptation to pathological states such as short gut syndrome, ulcers, and inflammatory bowel disease. The rodent Slfn3 and its human analog Slfn12 are critical in regulating intestinal epithelial differentiation. We sought to characterize intestinal function in Slfn3 knockout (KO) mice. Male and female pair-fed Slfn3KO mice gained less weight with decreased food efficiency than wild type (WT) mice, with more pronounced effects in females. RNA sequencing performed on intestinal mucosa of Slfn3KO and WT mice showed gene ontology decreases in cell adhesion molecule signaling, tumor necrosis factor receptor binding, …

Bird Species And Abundances In Fruit Crops And Implications For Bird Management, Melissa B. Hanney, Jason R. Boulanger, Paul D. Curtis, Rachael A. Eaton, Benjamin C. Hawes, Deanna K. Leigh, Cody A. Rossetti, Karen M. M. Sttensma, Catherine A. Lindell

Biology Faculty Publications

Fruit consumption by birds is a costly problem in North America, yet basic information about the species and abundance of fruit-eating birds in fruit crops, and factors that influence abundance, are lacking. We conducted a study of fruit-eating birds in 'Honeycrisp' apples, blueberries, grapes, and sweet cherries in Michigan, New York, and the Pacific Northwest in 2012 and 2013. We documented the most frequently observed fruit-eating birds in each crop across our study regions, and used fruit-consumption data to identify bird species for each crop and region that have a great impact via fruit consumption. We found that American Robins …

Color And Pigment Polymorphisms Of Northern Leopard Frogs On A Prairie Landscape, Kyle D. Gustafson, Bartholomew L. Bly, Robert A. Newman

Biology Faculty Publications

Variation allows populations to adapt to changing conditions. As human activities continue to alter environments and evolutionary processes, it becomes increasingly important to conserve standing genetic variation. Despite technical advances in population genetics, it is still useful to have inexpensive methods of detecting and monitoring genetic variation, particularly in traits that potentially influence fitness. In the Northern Leopard Frog, Lithobates pipiens (= Rana pipiens), genetically determined color (green [dominant: G] or brown [recessive: g]) and two pigment pattern polymorphisms (Burnsi/spotless [B] or spotted [b]; Kandiyohi/mottled [K] or non-mottled[k]) are hypothesized to have adaptive benefits. We assessed spatiotemporal patterns of these …

Motivations And Satisfaction Of North Dakota Deer Hunters During A Temporal Decline In Deer Populations, Kristen E. Black, William F. Jensen, Robert Newman, Jason Boulanger

Biology Faculty Publications

Achieving state wildlife agency biological goals for deer (Odocoileus spp.) management may often conflict with hunter desires. Concomitantly, better information is needed to optimize agency deer herd management goals with hunters’ social goals. In 2016, we surveyed 3,000 North Dakota, USA, resident deer hunters using a self-administered mail survey to gain a better understanding of motivations, satisfaction, and hunter demographics that may be used to inform hunter recruitment and retention (HRR) efforts during a period of reduced statewide deer populations. With deer-gun license availability strictly limited, we explored the possibility that some gun hunters may have been engaging …

Genetic Diversity And Origin Of The Feral Horses In Theodore Roosevelt National Park, Igor V. Ovtchinnikov, Taryn Dahms, Billie Herauf, Blake Mccann, Rytis Juras, Caitlin Castaneda, E. Gus Cothran

Biology Faculty Publications

Feral horses in Theodore Roosevelt National Park (TRNP) represent an iconic era of the North Dakota Badlands. Their uncertain history raises management questions regarding origins, genetic diversity, and long-term genetic viability. Hair samples with follicles were collected from 196 horses in the Park and used to sequence the control region of mitochondrial DNA (mtDNA) and to profile 12 autosomal short tandem repeat (STR) markers. Three mtDNA haplotypes found in the TRNP horses belonged to haplogroups L and B. The control region variation was low with haplotype diversity of 0.5271, nucleotide diversity of 0.0077 and mean pairwise difference of 2.93. We …

Differential Susceptibilities Of Anopheles Albimanus And Anopheles Stephensi Mosquitoes To Ivermectin, Staci M. Dreyer, Kelsey J. Morin, Jefferson A. Vaughan

Biology Faculty Publications

Background

Vector control is a crucial element of anti-malaria campaigns and works best when there is a thorough knowledge of the biology and behaviour of the Anopheles vector species responsible for transmitting malaria within a given locale. With the push to eradicate malaria stronger than ever, there is a growing need to develop and deploy control strategies that exploit the behavioural attributes of local vector species. This is especially true in regions where the vectors are exophagic (i.e., prefer to bite outdoors), exophilic (i.e., prefer to remain outdoors), and zoophagic (i.e., as likely to feed on non-humans as humans). One …

Cyclophyllidea Van Beneden In Braun, 1900, Jean Mariaux, Vasyl V. Tkach, Gergana P. Vasileva, Andrea Waeschenbach, Ian Beveridge, Yana D. Dimitrova, Voitto Haukisalmi, Stephen E. Greiman, D. Timothy J. Littlewood, Arseny A. Makarikov, Anna J. Phillips, Tantely Razafiarisolo, Vincent Widmer, Boyko B. Georgiev

Biology Faculty Publications

No abstract provided.

Sharp-Tailed Grouse Nest Survival And Nest Predator Habitat Use In North Dakota’S Bakken Oil Field, Paul C. Burr, Aaron C. Robinson, Randy T. Larsen, Robert A. Newman, Susan N. Ellis-Felege

Biology Faculty Publications

Recent advancements in extraction technologies have resulted in rapid increases of gas and oil development across the United States and specifically in western North Dakota. This expansion of energy development has unknown influences on local wildlife populations and the ecological interactions within and among species. Our objectives for this study were to evaluate nest success and nest predator dynamics of sharp-tailed grouse (Tympanuchus phasianellus) in two study sites that represented areas of high and low energy development intensities in North Dakota. During the summers of 2012 and 2013, we monitored 163 grouse nests using radio telemetry. Of these, …

Increased Bufflehead (Bucephala Albeola) Breeding Activity In Minnesota, Susan N. Ellis-Felege, Cassandra G. Skaggs, Gregory A. Knutsen

Biology Faculty Publications

Buffleheads (Bucephala albeola) predominantly nest in the boreal forests and aspen parklands of Canada and Alaska. Historically, Buffleheads were common migrants but not summer residents in Minnesota. However, recent observations in Minnesota and surrounding states suggest increased breeding activity in the region. In 1978, the first Bufflehead brood in Minnesota was recorded at East Park Wildlife Management Area. Annually, Agassiz National Wildlife Refuge (NWR) has conducted pair and brood surveys, with species-specific records available from 1990 to 2014. We report an increase in numbers of Bufflehead breeding pairs and broods at Agassiz NWR and new pair observations in …

Landscape Features Affecting Northern Bobwhite Predator-Specific Nest Failures In Southeastern Usa, Susan N. Ellis-Felege, Shannon E. Albeke, Nathan P. Nibbelink, Michael J. Conroy, Clay Sisson, William E. Palmer, John P. Carroll

Biology Faculty Publications

Nest predation is a critical component in avian productivity and typically is the leading cause of nest failure for most birds. Several landscape features are thought to drive the behavioral interaction between northern bobwhite (Colinus virginianus; e.g., nest placement) and their predators (e.g., search methods for food acquisition). In order to understand habitat characteristics influencing predation, we studied bobwhite nests using 24-hour near-infrared video cameras. We monitored 675 bobwhite nests with cameras on 3 properties in northern Florida and southern Georgia, USA, during 2000–2006. To test the association between nest failures and specific failure causes with landscape structure, we calculated …

App Regulates Microglial Phenotype In A Mouse Model Of Alzheimer's Disease, Gunjan D. Manocha, Angela M. Floden, Keiko Rausch, Joshua A. Kulas, Brett A. Mcgregor, Lalida Rojanathammanee, Kelley R. Puig, Kendra L. Puig, Sanjib Karki, Michael R. Nichols, Diane C. Darland, James E. Porter, Colin K. Combs

Biology Faculty Publications

Prior work suggests that amyloid precursor protein (APP) can function as a proinflammatory receptor on immune cells, such as monocytes and microglia. Therefore, we hypothesized that APP serves this function in microglia during Alzheimer's disease. Although fibrillar amyloid β (Aβ)-stimulated cytokine secretion from both wild-type and APP knock-out (mAPP−/−) microglial cultures, oligomeric Aβ was unable to stimulate increased secretion from mAPP−/− cells. This was consistent with an ability of oligomeric Aβ to bind APP. Similarly, intracerebroventricular infusions of oligomeric Aβ produced less microgliosis in mAPP−/− mice compared with wild-type mice. The mAPP−/− mice crossed to an APP/PS1 transgenic mouse line …

Physiological Perturbation Reveals Modularity Of Eyespot Development In The Painted Lady Butterfly, Vanessa Cardui, Heidi Connahs, Turk Rhen, Rebecca Simmons

Biology Faculty Publications

Butterfly eyespots are complex morphological traits that can vary in size, shape and color composition even on the same wing surface. Homology among eyespots suggests they share a common developmental basis and function as an integrated unit in response to selection. Despite strong evidence of genetic integration, eyespots can also exhibit modularity or plasticity, indicating an underlying flexibility in pattern development. The extent to which particular eyespots or eyespot color elements exhibit modularity or integration is poorly understood, particularly following exposure to novel conditions. We used perturbation experiments to explore phenotypic correlations among different eyespots and their color elements on …

Digital Fragment Analysis Of Short Tandem Repeats By High‐Throughput Amplicon Sequencing, Brian J. Darby, Shay F. Erickson, Samuel D. Hervey, Susan N. Ellis-Felege

Biology Faculty Publications

High‐throughput sequencing has been proposed as a method to genotype microsatellites and overcome the four main technical drawbacks of capillary electrophoresis: amplification artifacts, imprecise sizing, length homoplasy, and limited multiplex capability. The objective of this project was to test a high‐throughput amplicon sequencing approach to fragment analysis of short tandem repeats and characterize its advantages and disadvantages against traditional capillary electrophoresis. We amplified and sequenced 12 muskrat microsatellite loci from 180 muskrat specimens and analyzed the sequencing data for precision of allele calling, propensity for amplification or sequencing artifacts, and for evidence of length homoplasy. Of the 294 total alleles, …

Species-Recognition Program: A Computer-Assisted Approach To Recognizing Species, Steve Kelsch, Jeffrey Carmichael

Biology Faculty Publications

Species recognition is a crucial component for many types of biological studies. To that end, broadly trained students must be able to accurately identify many different types of organisms. Courses that focus on learning the names of different species traditionally rely on preserved specimens viewed during class or laboratory time. Unfortunately, reliance on preserved specimens comes with many challenges in providing students with an optimal learning experience. The curriculum activity described here uses a modified PowerPoint file (species-recognition program—SRP) as a means of helping students learn to recognize and identify fishes based on subtle visual cues. Our results indicate that …

Temporal And Spatial Dynamics Of Scaling-Specific Features Of A Gene Regulatory Network In Drosophila, Honggang Wu, Renjie Jiao, Manu, Jun Ma

Biology Faculty Publications

A widely appreciated aspect of developmental robustness is pattern formation in proportion to size. But how such scaling features emerge dynamically remains poorly understood. Here we generate a data set of the expression profiles of six gap genes in Drosophila melanogasterembryos that differ significantly in size. Expression patterns exhibit size-dependent dynamics both spatially and temporally. We uncover a dynamic emergence of under-scaling in the posterior, accompanied by reduced expression levels of gap genes near the middle of large embryos. Simulation results show that a size-dependent Bicoid gradient input can lead to reduced Krüppel expression that can have long-range and …

Fight Or Flight: Parental Decisions About Predators At Nests Of Northern Bobwhites (Colinus Virginianus), Susan N. Ellis-Felege, Jonathan S. Burnam, William E. Palmer, D. Clay Sisson, John P. Carroll

Biology Faculty Publications

Patterns of nest defense against predators by ground-nesting bird species in the wild are poorly understood, largely because of a historical inability to directly monitor nests. Most nest-defense studies have observed responses elicited from artificial predators or human observers presented to nesting birds, and few have attempted to present these events in the context of predator—prey relationships found in the wild. We hypothesized that predator threat level (e.g., the threat posed to the clutch or to the clutch and the attending adult), parental characteristics, clutch investment, and future reproductive opportunities would influence avian nest-defense decisions. During 1999–2006, we examined predation …

Sterilization As An Alternative Deer Control Technique: A Review, Jason R. Boulanger, Paul D. Curtis, Evan G. Cooch, Anthony J. Denicola

Biology Faculty Publications

Burgeoning white-tailed deer (Odocoileus virginianus) populations in suburban landscapes continue to impact communities and challenge natural resource managers. Increased deer-related damage to vegetation, ecosystems, and automobiles can exceed the tolerance of local stakeholders. We provide an overview of the potential efficacy of using surgical sterilization to help manage populations and conflicts associated with locally overabundant white-tailed deer populations. We review theoretical and fi eld studies pertaining to deer sterilization, and provide research priorities to help guide future sterilization efforts. Recent fi eld studies suggest that sterilization of female deer remains expensive, at approximately $1,000 per surgery. Sterilization may …

Relative Effectiveness Of Repellents For Preventing Deer Damage To Japanese Yews, Paul D. Curtis, Jason R. Boulanger

Biology Faculty Publications

Homeowners whose landscape plants are repeatedly browsed by white-tailed deer (Odocoileus virginianus) are interested in repellent products that are effective and long-lasting. New products come to market with limited experimental testing. We conducted a 10-week trial from Feb. through Apr. 1999 to test the duration and efficacy of six commercial deer repellents [Deer-Away Big Game Repellent (BGR) mix, BGR spray, Deer-Off, Deer Stopper II, Repellex, Tree Guard] and two experimental deer repellents (CU-A and CU-B) relative to each other and to untreated plants. Treated and control balled japanese yew (Taxus cuspidata) shrubs were placed at each …

Integrating Lethal And Nonlethal Approaches For Management Of Suburban Deer, Jason R. Boulanger, Paul D. Curtis, Michael Ashdown

Biology Faculty Publications

Evaluations of white-tailed deer (Odocoileus virginianus) population management in suburban landscapes has included debate over lethal control (e.g., sharp-shooting and hunting). These management techniques are often stymied by political impediments, safety concerns, and public attitudes. We are implementing the novel use of surgical sterilization in combination with hunting to mitigate deer-related impacts on Cornell University lands near Ithaca, New York. The project lands are composed of 2 zones: a suburban core campus area (446 ha) and adjacent outlying areas that contain agricultural fields and natural areas where deer hunting is permitted (582 ha). Surgical sterilization will be the …

Estimating Deer Abundance In Suburban Areas With Infrared-Triggered Cameras, Paul D. Curtis, Bazartseren Boldgiv, Peter M. Mattison, Jason R. Boulanger

Biology Faculty Publications

Interactions between humans and white-tailed deer (Odocoileus virginianus) have become common, often resulting in management actions to mitigate negative impacts. Changes in population size are generally used to judge management actions. We examined deer population estimation techniques during 2 mark-resighting experiments in a woodland-grassland habitat in central New York State and in a suburban area. We compared program NOREMARK, Lincoln-Peterson estimates, and Jacobson’s (1997) buck:doe ratios (BDR) for estimating deer abundance. In the first field trial, we sought to validate the camera survey methods and computer applications. We used infrared-triggered cameras (IRCs) to survey a white-tailed deer population …