Life Sciences Commons^™

Description Of Neoperla Philippinensis Sp. Nov. Confirmed Using Integrative Taxonomy And Some Notes On Neoperla Oculata Banks, 1924 (Plecoptera: Perlidae), Arthien Lovell P. Pelingen

Biology Faculty Publications

I re-examine the assumed phenotypic variable Neoperla oculata Banks, 1924 (Plecoptera, Perlidae) and describe Neoperla philippinensis sp. nov. All material examined herein are collections from the Mindanao region, of which supplementary information were provided including COI mtDNA accessions, female adult associations, and egg morphology characterization.

Growth And Whole-Body Proximate Composition Of Oreochromis Niloticus Nile Tilapia Fed Pea Meal: A Systematic Review And Meta-Analysis, Tzodoq Obrero Magbanua, Janice A. Ragaza

Biology Faculty Publications

One of the most widely cultivated species in the aquaculture sector is the Nile tilapia (Oreochromis niloticus). However, the rising price of fishmeal has a huge impact on its market value. Proteins derived from plants have been used to address the issue. In this study, systematic review and meta-analysis were used to quantitatively analyze the whole-body composition and growth of Nile tilapia fed pea meal. Based on the preferred reporting items for systematic reviews and meta-analysis methods, the literature review and paper selection were completed. Meta-analysis was performed on the data from peer-reviewed studies that met the inclusion …

Systematic Review And Meta-Analysis Of The Growth Performance And Carcass Composition Of Nile Tilapia (Oreochromis Niloticus) Fed Dietary Copra Meal, Tzodoq Obrero Magbanua, Janice A. Ragaza

Biology Faculty Publications

Introduction

Nile tilapia (Oreochromis niloticus) is one of the most cultured species in the aquaculture industry. However, its market price is affected by the increasing cost of fishmeal. Plant-based proteins are being used to mitigate the problem.

Methodology

This paper comprehensively quantified the growth performance and carcass composition of Nile tilapia fed copra meal via systematic review and meta-analysis. The literature review and paper selection were done based on the preferred reporting items for systematic reviews and meta-analysis method. The data from peer-reviewed papers that qualified from the inclusion screening criteria such as standard error reporting, feeding period, absence of …

Selected Dietary Plant-Based Proteins For Growth And Health Response Of Nile Tilapia Oreochromis Niloticus, Tzodoq O. Magbanua, Janice A. Ragaza

Biology Faculty Publications

Tilapia culture is one of the largest sectors of global aquaculture. Among the different species of tilapia, Nile tilapia (Oreochromis niloticus) is perhaps the top cultured species. The production of Nile tilapia has been continually increasing throughout the years resulting in genetic deterioration. Several tilapia strains with better growth performance and adaptive capability to survive in different culture conditions have been developed to alleviate the crisis. Increased demand for Nile tilapia implies higher farming cost. Plant-based proteins are utilized as partial or complete fishmeal replacements to reduce feed cost. However, these proteins can adversely affect and alter growth …

A Global Agenda For Advancing Freshwater Biodiversity Research, Alain Maasri, Sonja C. Jähnig, Hendrik Freitag, 93 Co-Authors

Biology Faculty Publications

Global freshwater biodiversity is declining dramatically, and meeting the challenges of this crisis requires bold goals and the mobilisation of substantial resources. While the reasons are varied, investments in both research and conservation of freshwater biodiversity lag far behind those in the terrestrial and marine realms. Inspired by a global consultation, we identify 15 pressing priority needs, grouped into five research areas, in an effort to support informed stewardship of freshwater biodiversity. The proposed agenda aims to advance freshwater biodiversity research globally as a critical step in improving coordinated actions towards its sustainable management and conservation.

Prevalence Of Cymothoidae (Isopoda) Infestation In Bigeye Scad (Selar Crumenophtalmus) From Batangas, Philippines, Tanya Faye S. Muji, Joaquin R. Sorreta, Janice A. Ragaza

Biology Faculty Publications

Cymothoid isopod research is relatively scarce in the Philippines, despite the local economic significance of bigeye scad as an inexpensive source of protein and other nutrients. Isopod parasitism has also been shown to have detrimental effects on their fish hosts. The current study aimed to define the host-parasite relationship between cymothoid isopod and bigeye scad by determining cymothoid isopod prevalence, intensity, and host-parasite length correlations in bigeye scad (Selar crumenophthalmus) hosts sourced from Batangas, Philippines. Fish samples were sampled from the Tagaytay City Market in Cavite, which sources fish directly from Batangas. Fish samples were immediately measured and …

Contributions To The Knowledge Of Water Bugs In Mindoro Island, Philippines, With A Species Checklist Of Nepomorpha And Gerromorpha (Insecta, Hemiptera, Heteroptera), Arthien Lovell Pelingen, Herbert Zettel, Clister V. Pangantihon, Kyra Mari Dominique Aldaba, Earl Kevin Fatallo, Jemillie Madonna De Leon, Hendrik Freitag

Biology Faculty Publications

This survey aims to provide an updated species checklist of aquatic and semi-aquatic bugs in the intra-Philippine biogeographic Region of Mindoro. An assessment survey of water bugs (Hemiptera, Heteroptera) was conducted mostly by manual collection in selected areas of Oriental Mindoro from 2017 to 2018, in which some of the collecting activities were undertaken by graduate students of Ateneo de Manila University. Twenty-nine aquatic and semi-aquatic heteropteran species were documented and some are known island-endemic species or subspecies, including Enithares martini mindoroensis Nieser & Zettel, 1999, Hydrotrephes stereoides mindoroensis Zettel, 2003, Aphelocheirus freitagi Zettel & Pangantihon, 2010, Rhagovelia mindoroensis Zettel, …

The Potential Impacts Of Soy Protein On Fish Gut Health, Vikas Kumar, Sakhawat Hossain, Janice A. Ragaza, Marina Rubio Benito

Biology Faculty Publications

Soy protein is the major source of protein as fishmeal replacement in fish feed because of its worldwide availability and low price. However, the presence of high carbohydrate content along with saponins, lectins, and phytates can have a negative impact on fish gut health. Based on the literature and our lab studies, dietary soybean meal can cause a dose-dependent type of distal intestine inflammation called enteritis in commercial fish species including salmonids. This leads to reduced absorptive capacity, increased mucus secretion, hyperpermeability, and leucocyte infiltration in the lamina propria and submucosa, also inducing the pro-inflammatory cytokine genes expression, including Il-1β, …

Growth And Immune Response Of Pond-Reared Giant Freshwater Prawn Macrobrachium Osenbergii Post Larvae Fed Diets Containing Chlorella Vulgaris, Gian Carlo F. Maliwat, Stephanie F. Velasquez, Sheila Marie D. Buluran, Melchor Tayamen, Janice A. Ragaza

Biology Faculty Publications

A 70-day feeding trial was conducted to evaluate the efficacy of Chlorella vulgaris supplementation in diets of giant freshwater prawn (Macrobrachium rosenbergii) post larvae. Resistance of the prawns against Aeromonas hydrophila and several immune parameters (total hemocyte count and prophenoloxidase activity) were also assessed in the study. Iso-nitrogenous, iso-lipidic, and iso-caloric prawn feeds were prepared utilizing a fishmeal based positive control diet (F0) and four dietary treatments with C. vulgaris inclusion levels of 2 (F2), 4 (F4), 6 (F6), and 8 (F8) %. Post larvae of M. rosenbergii were stocked randomly (mean initial body weight of 0.39 ± …

Fish Performance, Nutrient Digestibilities, And Hepatic And Intestinal Morphologies In Grouper Epinephelus Fuscoguttatus Fed Fermented Copra Meal, Roger Edward P. Mamauag, Janice A. Ragaza, Trisha J. Nacionales

Biology Faculty Publications

Protein enhanced copra meal (PECM®) is an alternative, cheap, and sustainable source of plant protein for the aquafeed industry, albeit its use on carnivorous fish species has been very limited. A 70-day feeding trial using grouper Epinephelus fuscoguttatus (initial mean body weight of 1.86 ± 0.19 g) tested fermented copra meal as feed ingredient. Six isonitrogenous (crude protein of 45%) and iso-lipidic (crude fat of 11%) diets consisted of PECM®: a control diet at 0% soybean meal replacement (C); four diets replacing soybean meal at 25% (FC25), 50% (FC50), 75% (FC75), 100% (FC100) – all with methionine and lysine supplementation; …

Replacement Of Fishmeal With Processed Meal From Knife Fish Chitala Ornata In Diets Of Juvenile Nile Tilapia Oreochromis Niloticus, Janice A. Ragaza, Sherilyn T. Abarra, Stephanie F. Velasquez, Kristina Daniela D.C. Guzman, Jaime Lorenzo F. Felipe, Melchor Tayamen

Biology Faculty Publications

A 60-day feeding trial was conducted to assess the effects of processed meal from knife fish Chitala ornata (KFM) as fishmeal replacement in diets of Nile tilapia Oreochromis niloticus juveniles. Five iso-nitrogenous (36.4% in dry matter) and isolipidic diets (8.6% in dry matter) with 0 (D1), 25 (D2), 50 (D3), 75 (D4) and 100% (D5) KFM inclusions were prepared. With a stocking density of 15 fish (0.59 ± 0.01 g) per tank, tilapia juveniles were distributed randomly in fifteen 30-L tanks. Results indicate a significant increase (P < 0.05) in the percent average weight gain, specific growth rate, and feed …

Quorum Quenching Activity Of Lysates From Bioluminescent Bacteria Isolated From Selected Philippine Marine And Freshwater Organisms, Janice A. Ragaza, Roja G. Angeles-Gumban, Mudjekeewis D. Santos, Minerva Fatimae H. Ventolero, Ronie J. Calugay

Biology Faculty Publications

Pathogens use cell density dependent gene expression known as Quorum Sensing (QS) to launch virulent factors. Targeting the QS circuit without killing the pathogens is a very promising alternative to using antibiotics which generate antibiotic resistance. The identity and diversity of bioluminescent bacteria associated with Philippine aquatic organisms, and their potential as sources of anti-QS or quorum quenching (QQ) molecules remain unknown. In this study, out of 43 organisms, 30 were observed to harbor bioluminescent bacteria with 26 from marine and four from freshwater sources. Purified bioluminescent bacterial cultures were screened for QQ activity using motility assay and the membrane …

Challenges And Opportunities In The Culture Of Giant Freshwater Prawn In The Philippines, Janice A. Ragaza

Biology Faculty Publications

Amid the irremediable decline of capture fisheries, the Philippine government has identified aquaculture as a sector that will ensure food security and generate new job opportunities geared toward the country’s goal of economic development (Nagothu and Ortiz 2007). Aquaculture of giant freshwater prawn Macrobrachium rosenbergii (Fig. 1) is an emerging program that is intended to expand and vary the aquatic species used in freshwater aquaculture, which is still predominantly based on tilapia (Rosario and Tayamen 2004). Attractive characteristics of freshwater prawn culture include ease of breeding, high hatching rate, superior quality of meat and high market value (Keysami and Mohammadpour …

Two New Species Of Geosesarma De Man, 1892 (Crustacea: Brachyura: Sesarmidae) From Palawan, The Philippines, Hendrik Freitag, Marivene Manuel-Santos, Peter K.L Ng

Biology Faculty Publications

Two new and relatively large species of semi-terrestrial sesarmid crabs of the genus Geosesarma De Man, 1892, are described from Palawan Island, Philippines. They are distinguishable from congeners by the characteristic structure of their carapace, chelipeds, ambulatory legs and male first gonopods. Aspects of their ecology are briefly discussed

A Fish Farmer’S Role In Sustainable Aquaculture: An Overview Of Philippine Aquaculture, Janice A. Ragaza, Jonni Fay C. Teves

Biology Faculty Publications

The expansion of Philippine aquaculture is essential to addressing poverty and food insecurity in the midst of a seemingly irreversible decline in national capture fisheries. Concomitant with its rise, however, are compounding environmental problems caused by climate change, lackluster governance and irresponsible farming practices. It is equally important to ensure aquaculture sustainability so that fish demand from a rapidly growing population is continually met and that fish farmers’ socioeconomic well-being is secured. As such, programs have been developed that aim to enjoin the government and private sectors with fishing communities to realize this goal.

Phylogenetic Analysis Of Algal Symbionts Associated With Four North American Amphibian Egg Masses, Eunsoo Kim, Yuan Lin, Ryan R. Kerney, Lili Blumenberg, Cory Bishop

Biology Faculty Publications

Egg masses of the yellow-spotted salamander Ambystoma maculatum form an association with the green alga “Oophila amblystomatis” (Lambert ex Wille), which, in addition to growing within individual egg capsules, has recently been reported to invade embryonic tissues and cells. The binomial O. amblystomatis refers to the algae that occur in A. maculatum egg capsules, but it is unknown whether this population of symbionts constitutes one or several different algal taxa. Moreover, it is unknown whether egg masses across the geographic range of A. maculatum, or other amphibians, associate with one or multiple algal taxa. To address these …

Hydrolyzed Tuna Meat By-Product Supplement For Juvenile Red Sea Bream, Pagrus Major, And Its Effect On Growth, Enzyme Activity, Plasma Parameters, And Apparent Nutrient Digestibility, Janice A. Ragaza, Roger Edward P. Mamauag, Shunsuke Koshio, Manabu Ishikawa, Saichiro Yokoyama

Biology Faculty Publications

A growth experiment was conducted on juvenile red sea bream, Pagrus major to investigate the effect of the inclusion in fish diets, of tuna meat by-product hydrolysate which was processed through enzymatic hydrolysis using a commercially available enzyme, derived from Bacillus subtilis. Six experimental diets were formulated in the experiment. Three diets contained 50, 150 and 250 g/kg of TPM-H (tuna meat by-product hydrolysate), and two diets with the unprocessed TPM (tuna meat by-product) at an inclusion level of 50 and 250 g/kg. A control diet was formulated without any addition of the test ingredients. Treatment diets were fed ad …

Schooling Increases Risk Exposure For Fish Navigating Past Artificial Barriers, Bertrand H. Lemasson, James W. Haefner, Mark D. Bowen

Biology Faculty Publications

Artificial barriers have become ubiquitous features in freshwater ecosystems and they can significantly impact a region's biodiversity. Assessing the risk faced by fish forced to navigate their way around artificial barriers is largely based on assays of individual swimming behavior. However, social interactions can significantly influence fish movement patterns and alter their risk exposure. Using an experimental flume, we assessed the effects of social interactions on the amount of time required for juvenile palmetto bass (Morone chrysops × M. saxatilis) to navigate downstream past an artificial barrier. Fish were released either individually or in groups into the flume …

Effects Of Replacing Fishmeal With Squash Seed Meal (Cucurbita Maxima) On Performance Of Juvenile Nile Tilapia (Oreochromis Niloticus), Janice A. Ragaza, Jonni Fay C. Teves, Marie Noelle Fernandez

Biology Faculty Publications

The effects of replacing fishmeal with squash (Cucurbita maxima) seed meal (SSM) on Oreochromis niloticus juveniles were investigated on a 30-day feeding trial. Triplicate groups of ten (10) O. niloticus juveniles (2.54±0.949 g) stocked in polyethylene tanks received iso-nitrogenous diets with SSM inclusion levels of 0%, 5%, 10%, 15% and 20%. Fish fed 5% SSM inclusion level showed the highest average weight gain (202.25±36.26%) and specific growth rate (3.76±0.42%) among the fish fed SSM-containing diets. However, there were no significant differences between the average weight gain and specific growth rate of fish in all diets. Fish fed S5, S15 and …

A Dichotomous Key For The Identification Of Nine Salmonids Of The Inland Northwest Using Six Diagnostic Skull Bones : And Associated Equations To Estimate Total Length And Weight From Bones Ingested By Piscivores Or Found In Archeological Sites, Aaron G. Stroud, Allan T. Scholz, Fisheries Research Center (Cheney, Wash.)

Biology Faculty Publications

No abstract provided.

Fishes Of Eastern Washington: A Natural History, Volume 4, Allan T. Scholz, Josh Smith, Amanda Maifeld, Judy Mcmillan, Larry Conboy, Eastern Washington University. Department Of Biology., Eastern Washington University. University Graphics.

Biology Faculty Publications

Volume 4 of 4.

CHAPTER 6: KEY TO FAMILIES OF EASTERN WASHINGTON FISHES (REVISED), CHAPTER 18: FAMILY PERCOPSIDAE: TROUT-PERCHES, CHAPTER 19: FAMILY GADIDAE: CODS, CHAPTER 20: FAMILY POECILIIDAE: LIVEBEARERS, CHAPTER 21: FAMILY GASTEROSTEIDAE: STICKLEBACKS, CHAPTER 22: FAMILY COTTIDAE: SCULPINS, CHAPTER 23: FAMILY CENTRARCHIDAE: SUNFISHES, CHAPTER 24: FAMILY PERCIDAE: PERCH, CHAPTER 25: FISH WITH RARE OR UNCERTAIN STATUS IN EASTERN WASHINGTON, CHAPTER 26: LIMINOLOGY, WATER QUALITY, AND FISH HABITAT

Fishes Of Eastern Washington: A Natural History, Volume 1, Allan T. Scholz, Tyler Basler, Josh Smith, Judy Mcmillan, Larry Conboy, Eastern Washington University. Department Of Biology., Eastern Washington University. University Graphics.

Biology Faculty Publications

Volume 1 of 4.

CHAPTER 1: INTRODUCTION, CHAPTER 2: CHECKLISTS OF EXTANT AND FOSSIL FISHES FOUND IN EASTERN WASHINGTON, CHAPTER 3: THE DISCOVERERS, CHAPTER 4: GEOLOGY, CHAPTER 5: COLUMBIA RIVER BASIN HYDROLOGY AND FISH DISTRIBUTION IN EASTERN WASHINGTON

Fishes Of Eastern Washington: A Natural History, Volume 2, Allan T. Scholz, Tyler Basler, Josh Smith, Judy Mcmillan, Larry Conboy, Eastern Washington University. Department Of Biology., Eastern Washington University. University Graphics.

Biology Faculty Publications

Volume 2 of 4.

CHAPTER 6: KEY TO FAMILIES OF EASTERN WASHINGTON FISHES, CHAPTER 7: FAMILY PETROMYZONTIDAE: LAMPREYS, CHAPTER 8: FAMILY ACIPENSERIDAE: STURGEON, CHAPTER 9: FAMILY CLUPEIDAE: HERRINGS, CHAPTER 10: FAMILY CYPRINIDAE: CARPS AND MINNOWS, CHAPTER 11: Family Catostomidae: Suckers, CHAPTER 12: FAMILY ICTALURIDAE: BULLHEAD CATFISHES, CHAPTER 13: Family Esocidae: Pikes

Fishes Of Eastern Washington: A Natural History, Volume 3, Allan T. Scholz, Josh Smith, Amanda Maifeld, Judy Mcmillan, Larry Conboy, Eastern Washington University. Department Of Biology., Eastern Washington University. University Graphics.

Biology Faculty Publications

Volume 3 of 4.

CHAPTER 14: FAMILY SALMONIDAE: SALMON, TROUT, CHARR, WHITEFISH AND GRAYLINGS, CHAPTER 15: FAMILY SALMONIDAE (SUBFAMILY COREGONINAE): WHITEFISH, CHAPTER 16: FAMILY SALMONIDAE (SUBFAMILY MONINAE): SALMON, TROUT AND CHARR, CHAPTER 17: FAMILY SALMONIDAE (SUBFAMILY THYMALLINAE): GRAYLINGS

The Role Of Crassostrea Virginica Hemocytes In Shell Formation: Ex Vivo Mineral Deposition By Cultured Hemocytes, Emily A. Untener, Douglas C. Hansen, Karolyn Hansen

Biology Faculty Publications

Abstract of Technical Paper Presented at the 103rd Annual Meeting National Shellfisheries Association Baltimore, Maryland March 27–31, 2011

Rna Interference In Marine And Freshwater Sponges: Actin Knockdown In Tethya Wilhelma And Ephydatia Muelleri By Ingested Dsrna Expressing Bacteria, Ajna S. Rivera, April L. Hill, Et. Al.

Biology Faculty Publications

Background: The marine sponge Tethya wilhelma and the freshwater sponge Ephydatia muelleri are emerging model organisms to study evolution, gene regulation, development, and physiology in non-bilaterian animal systems. Thus far, functional methods (i.e., loss or gain of function) for these organisms have not been available.

Results: We show that soaking developing freshwater sponges in double-stranded RNA and/or feeding marine and freshwater sponges bacteria expressing double-stranded RNA can lead to RNA interference and reduction of targeted transcript levels. These methods, first utilized in C. elegans, have been adapted for the development and feeding style of easily cultured marine and freshwater poriferans. …

Fishes Of The Columbia And Snake River Basins In Eastern Washington, Allan T. Scholz, Holly J. Mclellan, Fisheries Research Center, Eastern Washington University

Biology Faculty Publications

"This book was prepared through a grant from the United States Department of the Interior, Bureau of Land Management (BLM)."

Chapter 1 Introduction, Chapter 2 Checklist of Fishes Found in Eastern Washington, Chapter 3 Fish Identification and Classification Aids to Identification of Fishes, Chapter 4 Key to Families of Eastern Washington Fishes, Chapter 5 Family Petromyzontidae: Lampreys, Chapter 6 Family Acipenseridae: Sturgeon, Chapter 7 Family Clupeidae: Herrings, Chapter 8 Family Cyprinidae: Carps and Minnows, Chapter 9 Family Catostomidae: Suckers, Chapter 10 Family Ictaluridae: Bullhead Catfishes, Chapter 11 Family Esocidae: Pikes, Chapter 12 Family Salmonidae: Salmon, Trout, Whitefish, Grayling, Chapter 13 …

Assessment Of Oyster Shell Structural Properties For The Development Of 'Green' Composite Materials, Yuhchae Yoon, Andrew S. Mount, Douglas C. Hansen, Karolyn Hansen

Biology Faculty Publications

Abstract of Technical Paper Presented at the 101^st Annual Meeting National Shellfisheries Association Savannah, Georgia March 22–26, 2009

Towards Biomimetic Ceramic Coatings: Cellular Aspects Of Oyster Shell Biomineralization, Mary Beth Johnstone, Karolyn Hansen, Neeraj V. Gohad, Douglas C. Hansen, Andrew S. Mount

Biology Faculty Publications

Abstract of Technical Paper Presented at the 101^st Annual Meeting National Shellfisheries Association Savannah, Georgia March 22–26, 2009

The Mineralization Front Of The Eastern Oyster Is Cellular, Andrew S. Mount, Neeraj V. Gohad, Mary Beth Johnstone, Karolyn Hansen, Douglas C. Hansen

Biology Faculty Publications

Abstract of Technical Paper Presented at the 101^st Annual Meeting National Shellfisheries Association Savannah, Georgia March 22–26, 2009