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Articles 1 - 30 of 53
Full-Text Articles in Life Sciences
Dysregulated Interferon Response Underlying Severe Covid-19, Leann Lopez, Peter C. Sang, Yun Tian, Yongming Sang
Dysregulated Interferon Response Underlying Severe Covid-19, Leann Lopez, Peter C. Sang, Yun Tian, Yongming Sang
Agricultural and Environmental Sciences Faculty Research
Innate immune interferons (IFNs), including type I and III IFNs, constitute critical antiviral mechanisms. Recent studies reveal that IFN dysregulation is key to determine COVID-19 pathogenesis. Effective IFN stimulation or prophylactic administration of IFNs at the early stage prior to severe COVID-19 may elicit an autonomous antiviral state, restrict the virus infection, and prevent COVID-19 progression. Inborn genetic flaws and autoreactive antibodies that block IFN response have been significantly associated with about 14% of patients with life-threatening COVID-19 pneumonia. In most severe COVID-19 patients without genetic errors in IFN-relevant gene loci, IFN dysregulation is progressively worsened and associated with the …
Non-Sterile Fermentation Of Food Waste Using Thermophilic And Alkaliphilic Bacillus Licheniformis Ynp5-Tsu For 2,3-Butanediol Production, Joshua A. O'Hair, Qing Jin, Dajun Yu, Jian Wu, Hengjian Wang, Suping Zhou, Haibo Huang
Non-Sterile Fermentation Of Food Waste Using Thermophilic And Alkaliphilic Bacillus Licheniformis Ynp5-Tsu For 2,3-Butanediol Production, Joshua A. O'Hair, Qing Jin, Dajun Yu, Jian Wu, Hengjian Wang, Suping Zhou, Haibo Huang
Agricultural and Environmental Sciences Faculty Research
Conversion of food waste into 2,3-butanediol (2,3-BDO) via microbial fermentation provides a promising way to reduce waste disposal to landfills and produce sustainable chemicals. However, sterilization of food waste, an energy- and capital-costly process, is generally required before fermentation to avoid any contamination, which reduces the energy net output and economic feasibility of food waste fermentation. In this study, we investigated the non-sterile fermentation of food waste to produce 2,3-BDO using a newly isolated thermophilic and alkaliphilic B. licheniformis YNP5-TSU. Three unitary food waste samples (i.e., pepper, pineapple, cabbage wastes) and one miscellaneous food waste mixture were respectively inoculated with …
Comparative Performance Of Reduced-Risk Fungicides And Biorational Products In Management Of Postharvest Botrytis Blight On Bigleaf Hydrangea Cut Flowers, Ravi Bika, Cristi Palmer, Lisa Alexander, Fulya Baysal-Gurel
Comparative Performance Of Reduced-Risk Fungicides And Biorational Products In Management Of Postharvest Botrytis Blight On Bigleaf Hydrangea Cut Flowers, Ravi Bika, Cristi Palmer, Lisa Alexander, Fulya Baysal-Gurel
Agricultural and Environmental Sciences Faculty Research
Botrytis cinerea is one of the problematic and notorious postharvest pathogens of bigleaf hydrangea (Hydrangea macrophylla) cut flowers. It causes flower blight, leaf blight, and stem rot, reducing the ornamental value (such as longevity, color, and texture) of flowers, ultimately making them unsalable. The objective of this study was to identify effective conventional fungicides and biorational products for botrytis blight management on bigleaf hydrangea cut flowers that can be easily and readily adopted by growers of ornamentals. Preventive preharvest whole-plant spray and postharvest dip treatment applications were used in this study. For the whole-plant spray applications, bigleaf hydrangea …
Immunogenetic Association Underlying Severe Covid-19, Kendall Mccoy, Autumn Peterson, Yun Tian, Yongming Sang
Immunogenetic Association Underlying Severe Covid-19, Kendall Mccoy, Autumn Peterson, Yun Tian, Yongming Sang
Agricultural and Environmental Sciences Faculty Research
SARS-CoV2 has caused the current pandemic of new coronavirus disease 2019 (COVID-19) worldwide. Clinical outcomes of COVID-19 illness range broadly from asymptotic and mild to a life-threatening situation. This casts uncertainties for defining host determinants underlying the disease severity. Recent genetic analyses based on extensive clinical sample cohorts using genome-wide association studies (GWAS) and high throughput sequencing curation revealed genetic errors and gene loci associated with about 20% of life-threatening COVID-19 cases. Significantly, most of these critical genetic loci are enriched in two immune signaling pathways, i.e., interferon-mediated antiviral signaling and chemokine-mediated/inflammatory signaling. In line with these genetic profiling studies, …
Multi-Year Incubation Experiments Boost Confidence In Model Projections Of Long-Term Soil Carbon Dynamics, Siyang Jian, Jianwei Li, Gangsheng Wang, Laurel A. Kluber, Christopher W. Schadt, Junyi Liang, Melanie A. Mayes
Multi-Year Incubation Experiments Boost Confidence In Model Projections Of Long-Term Soil Carbon Dynamics, Siyang Jian, Jianwei Li, Gangsheng Wang, Laurel A. Kluber, Christopher W. Schadt, Junyi Liang, Melanie A. Mayes
Agricultural and Environmental Sciences Faculty Research
Global soil organic carbon (SOC) stocks may decline with a warmer climate. However, model projections of changes in SOC due to climate warming depend on microbially-driven processes that are usually parameterized based on laboratory incubations. To assess how lab-scale incubation datasets inform model projections over decades, we optimized five microbially-relevant parameters in the Microbial-ENzyme Decomposition (MEND) model using 16 short-term glucose (6-day), 16 short-term cellulose (30-day) and 16 long-term cellulose (729-day) incubation datasets with soils from forests and grasslands across contrasting soil types. Our analysis identified consistently higher parameter estimates given the short-term versus long-term datasets. Implementing the short-term and …
First Report Of Powdery Mildew On Physocarpus Opulifolius Caused By Podosphaera Physocarpi In Tennessee, Fulya Baysal-Gurel, Terri Simmons, Farhat A. Avin, Ravi Bika, Christina Jennings
First Report Of Powdery Mildew On Physocarpus Opulifolius Caused By Podosphaera Physocarpi In Tennessee, Fulya Baysal-Gurel, Terri Simmons, Farhat A. Avin, Ravi Bika, Christina Jennings
Agricultural and Environmental Sciences Faculty Research
Eastern ninebark (Physocarpus opulifolius [L.] Maxim.) is a popular native perennial plant used in landscapes because of its colorful foliage and spring flower display. Powdery mildew symptoms were observed on container-grown eastern ninebark ‘Mindia’ Coppertina plants in a commercial nursery in DeKalb County, TN, in May 2016. The disease severity was nearly 40%, and the disease incidence was nearly 60% of 1,000 plants. Affected plants displayed witches’ brooms with cream to white colored, thickened shoots with stunted, curly leaves as well as patches of white powdery fungal growth on the surface of young and old leaves, inflorescences, infructescences, and stems. …
Effects Of Nitrogen Fertilization And Bioenergy Crop Species On Central Tendency And Spatial Heterogeneity Of Soil Glycosidase Activities, Min Yuan, Jianjun Duan, Jianwei Li, Siyang Jian, Lahiru Gamage, Kudjo E. Dzantor, Dafeng Hui, Philip A. Fay
Effects Of Nitrogen Fertilization And Bioenergy Crop Species On Central Tendency And Spatial Heterogeneity Of Soil Glycosidase Activities, Min Yuan, Jianjun Duan, Jianwei Li, Siyang Jian, Lahiru Gamage, Kudjo E. Dzantor, Dafeng Hui, Philip A. Fay
Agricultural and Environmental Sciences Faculty Research
Extracellular glycosidases in soil, produced by microorganisms, act as major agents for decomposing labile soil organic carbon (e.g., cellulose). Soil extracellular glycosidases are significantly affected by nitrogen (N) fertilization but fertilization effects on spatial distributions of soil glycosidases have not been well addressed. Whether the effects of N fertilization vary with bioenergy crop species also remains unclear. Based on a 3-year fertilization experiment in Middle Tennessee, USA, a total of 288 soil samples in topsoil (0–15 cm) were collected from two 15 m2 plots under three fertilization treatments in switchgrass (SG: Panicum virgatum L.) and gamagrass (GG: Tripsacum dactyloides L.) …
Impact Of Porcine Arterivirus, Influenza B, And Their Coinfection On Antiviral Response In The Porcine Lung, Damarius S. Fleming, Laura C. Miller, Yun Tian, Yonghai Li, Wenjun Ma, Yongming Sang
Impact Of Porcine Arterivirus, Influenza B, And Their Coinfection On Antiviral Response In The Porcine Lung, Damarius S. Fleming, Laura C. Miller, Yun Tian, Yonghai Li, Wenjun Ma, Yongming Sang
Agricultural and Environmental Sciences Faculty Research
Interferon (IFN) cytokines induce an autonomous antiviral state in cells of the infected site to restrict virus spreading and critically regulate overall antiviral response. The antiviral state leads to host protection through expression of hundreds of IFN-stimulated genes that restrict viral infection through multiple mechanisms, for example, directly in viral genome degradation and indirectly through cellular metabolic inhibition. Young pigs were split into four treatment groups: control, porcine reproductive and respiratory syndrome virus (PRRSV, also known as porcine arterivirus) infected, influenza B virus (IBV) infected, and IBV/PRRSV coinfection. Lung tissue was collected at 3, 5, and 7 days post infection …
Inoculation And Amendment Strategies Influence Switchgrass Establishment In Degraded Soil, Ekundayo Adeleke, Kudjo E. Dzantor, Ali Taheri
Inoculation And Amendment Strategies Influence Switchgrass Establishment In Degraded Soil, Ekundayo Adeleke, Kudjo E. Dzantor, Ali Taheri
Agricultural and Environmental Sciences Faculty Research
Bioenergy feedstock production on degraded land can serve as a means for modulating land competition for food versus energy. Due to little or no agricultural value of degraded soil, fortification of the soil with an organic amendment or inoculum will improve biomass productivity. However, as farmers struggle to rejuvenate their degraded land, there is a need for a quick screening strategy to select the best method of enhancing cellulosic (switchgrass, SG) biomass production in degraded soil. The goal of this study is to evaluate the effects of soil amendment and inoculation strategies on biomass productivities of SG in a reclaimed …
Impacts Of Organic And Conventional Management On The Nutritional Level Of Vegetables, Atanu Mukherjee, Emmanuel C. Omondi, Paul R. Hepperly, Rita Seidel, Wade P. Heller
Impacts Of Organic And Conventional Management On The Nutritional Level Of Vegetables, Atanu Mukherjee, Emmanuel C. Omondi, Paul R. Hepperly, Rita Seidel, Wade P. Heller
Agricultural and Environmental Sciences Faculty Research
The nutrient concentration of fruits and vegetables in the U.S.A. has declined in the past 50–70 years. Crop management practices utilizing on-farm inputs are thought to increase crop nutritional quality, but few studies have evaluated this under long-term side-by-side trials. An experiment was conducted from 2004 to 2005 at Rodale Institute’s long-term Farming Systems Trial to investigate the nutritional quality of vegetables under organic manure (MNR) and conventional (CNV) farming systems, with or without arbuscular mycorrhizal fungi (AMF) treatment. AMF reduced the vitamin C content in carrots in both systems in 2004, but the reduction was 87% in CNV and …
Comparative Performance Of Chemical And Biological-Based Products In Management Of Algal Leaf Spot On Magnolia, Fulya Baysal-Gurel, Ravi Bika, Christina Jennings, Cristi Palmer, Terri Simmons
Comparative Performance Of Chemical And Biological-Based Products In Management Of Algal Leaf Spot On Magnolia, Fulya Baysal-Gurel, Ravi Bika, Christina Jennings, Cristi Palmer, Terri Simmons
Agricultural and Environmental Sciences Faculty Research
Magnolia trees (Magnolia sp.) are a popular choice for consumers when choosing flowering woody plants for landscapes. Magnolia species grow in a wide variety of both temperate and tropical locations. Southern magnolia (Magnolia grandiflora) is one of the more popular magnolias due to its pleasing aesthetics: large showy flowers in a range of colors and evergreen foliage. However, magnolias can be affected by algal leaf spot. Algal leaf spot is caused by Cephaleuros virescens, which is a widespread plant parasitic green alga. There has been little research on how to treat algal leaf spot on magnolia …
A Mini-Tn5-Derived Transposon With Reportable And Selectable Markers Enables Rapid Generation And Screening Of Insertional Mutants In Gram-Negative Bacteria, Eric S. Nazareno, Bimala Acharya, C. Korsi Dumenyo
A Mini-Tn5-Derived Transposon With Reportable And Selectable Markers Enables Rapid Generation And Screening Of Insertional Mutants In Gram-Negative Bacteria, Eric S. Nazareno, Bimala Acharya, C. Korsi Dumenyo
Agricultural and Environmental Sciences Faculty Research
We re-engineered a classic tool for mutagenesis and gene expression studies in Gram-negative bacteria. Our modified Tn5-based transposon contains multiple features that allow rapid selection for mutants, direct quantification of gene expression and straightforward cloning of the inactivated gene. The promoter-less gfp-km cassette provides selection and reporter assay depending on the activity of the promoter upstream of the transposon insertion site. The cat gene facilitates positive antibiotic selection for mutants, while the narrow R6Kγ replication origin forces transposition in recipient strains lacking the pir gene and enables cloning of the transposon flanked with the disrupted gene from the chromosome. The …
Distinct Distribution Of Archaea From Soil To Freshwater To Estuary: Implications Of Archaeal Composition And Function In Different Environments, Hualong Wang, Raven Bier, Laura Zgleszewski, Marc Peipoch, Emmanuel Omondi, Atanu Mukherjee, Feng Chen, Chuanlun Zhang, Jinjun Kan
Distinct Distribution Of Archaea From Soil To Freshwater To Estuary: Implications Of Archaeal Composition And Function In Different Environments, Hualong Wang, Raven Bier, Laura Zgleszewski, Marc Peipoch, Emmanuel Omondi, Atanu Mukherjee, Feng Chen, Chuanlun Zhang, Jinjun Kan
Agricultural and Environmental Sciences Faculty Research
In addition to inhabiting extreme territories, Archaea are widely distributed in common environments spanning from terrestrial to aquatic environments. This study investigated and compared archaeal community structures from three different habitats (representing distinct environments): agriculture soils (from farming system trials FST, PA, United States), freshwater biofilms (from White Clay Creek, PA, United States), and estuary water (Chesapeake Bay, United States). High-throughput sequencing of 16S rRNA genes indicated that Thaumarchaeota, Euryarchaeota, Nanoarchaeota, Crenarchaeota, and Diapherotrites were the commonly found dominant phyla across these three environments. Similar to Bacteria, distinct community structure and distribution patterns for Archaea were observed in soils vs. …
Evaluation Of Systemic Imidacloprid And Herbicide Treatments On Flatheaded Borer (Coleoptera: Buprestidae) Management In Field Nursery Production, Karla M. Addesso, Jason B. Oliver, Nadeer N. Youssef, Donna C. Fare
Evaluation Of Systemic Imidacloprid And Herbicide Treatments On Flatheaded Borer (Coleoptera: Buprestidae) Management In Field Nursery Production, Karla M. Addesso, Jason B. Oliver, Nadeer N. Youssef, Donna C. Fare
Agricultural and Environmental Sciences Faculty Research
The flatheaded appletree borer, Chrysobothris femorata (Olivier) (Coleoptera: Buprestidae), and related species are deciduous tree pests. Female beetles prefer to oviposit at tree bases, and larvae tunnel beneath the bark, which weakens or kills young or newly transplanted trees. In the first objective of this study, Discus N/G (2.94% imidacloprid + 0.7% cyfluthrin) applied at six lower-than-labeled rates (0.0, 0.98, 1.97, 3.94, 5.91, and 7.87 ml/cm of average trunk dia.) was evaluated for protection of field-grown maples. A second objective evaluated imidacloprid with and without herbicides to assess the impact of weed competition at the tree base on insecticide effectiveness. …
Urban Agriculture In Asia To Meet The Food Production Challenges Of Urbanization: A Review, Onyekachukwu Akaeze, Dilip Nandwani
Urban Agriculture In Asia To Meet The Food Production Challenges Of Urbanization: A Review, Onyekachukwu Akaeze, Dilip Nandwani
Agricultural and Environmental Sciences Faculty Research
Urban expansion in Asia is concentrated in metropolitan areas. This has adversely affected in-city food production by decreasing farmlands in and around urban centers. Asian countries have experienced fast disappearance of urban-fringe farmland which at some point provided 70% of the vegetables consumed by the city's population. Uneven distribution of incomes, along with an increase in urban poverty, has further exacerbated the already critical problems of low quantity and quality and high prices of wholesome food. In India, it is estimated that five out of every six urban families typically spend 70% of their income on food. The United Nations …
Introgression Of The Afila Gene Into Climbing Garden Pea (Pisum Sativum L.), Oscar Eduardo Checa, Marino Rodriguez, Xingbo Wu, Matthew Wohlgemuth Blair
Introgression Of The Afila Gene Into Climbing Garden Pea (Pisum Sativum L.), Oscar Eduardo Checa, Marino Rodriguez, Xingbo Wu, Matthew Wohlgemuth Blair
Agricultural and Environmental Sciences Faculty Research
The pea (Pisum sativum L.) is one of the most important crops in temperate agriculture around the world. In the tropics, highland production is also common with multiple harvests of nearly mature seeds from climbing plant types on trellises. While the leafless variant caused by the afila gene is widely used in developing row-cropped field peas in Europe, its use for trellised garden peas has not been reported. In this study we describe a pea breeding program for a high-elevation tropical environment in the Department of Nariño in Colombia, where over 16,000 hectares of the crop are produced. The most …
Genotypic Variation Of Flavonols And Antioxidant Capacity In Broccoli, Yongbo Duan, Franklin Eduardo Melo Santiago, Andre Rodrigues Dos Reis, Marislaine A. De Figueiredo, Suping Zhou, Theodore W. Thannhauser, Li Li
Genotypic Variation Of Flavonols And Antioxidant Capacity In Broccoli, Yongbo Duan, Franklin Eduardo Melo Santiago, Andre Rodrigues Dos Reis, Marislaine A. De Figueiredo, Suping Zhou, Theodore W. Thannhauser, Li Li
Agricultural and Environmental Sciences Faculty Research
Flavonols are gaining increasing interests due to their diverse health benefits for humans. Broccoli is a main flavonol source in our diet, but the genetic variation of flavonols and their correlation with antioxidant capacity remain to be understood. Here, we examined variations of the two major flavonols kaempferol and quercetin in florets and leaves of 15 diverse broccoli accessions by ultra-performance liquid chromatography. Broccoli accumulated more kaempferol than quercetin in most of the accessions tested, with the ratios varying from 4.4 to 27.9 in leaves and 0.4 to 4.4 in florets. Total flavonoids showed 2.5-fold and 3.3-fold differences in leaves …
Genomic Modeling As An Approach To Identify Surrogates For Use In Experimental Validation Of Sars-Cov-2 And Hunov Inactivation By Uv-C Treatment, Brahmaiah Pendyala, Ankit Patras, Bharat Pokharel, Doris D’Souza
Genomic Modeling As An Approach To Identify Surrogates For Use In Experimental Validation Of Sars-Cov-2 And Hunov Inactivation By Uv-C Treatment, Brahmaiah Pendyala, Ankit Patras, Bharat Pokharel, Doris D’Souza
Agricultural and Environmental Sciences Faculty Research
Severe Acute Respiratory Syndrome coronavirus-2 (SARS-CoV-2) is responsible for the COVID-19 pandemic that continues to pose significant public health concerns. While research to deliver vaccines and antivirals are being pursued, various effective technologies to control its environmental spread are also being targeted. Ultraviolet light (UV-C) technologies are effective against a broad spectrum of microorganisms when used even on large surface areas. In this study, we developed a pyrimidine dinucleotide frequency based genomic model to predict the sensitivity of select enveloped and non-enveloped viruses to UV-C treatments in order to identify potential SARS-CoV-2 and human norovirus surrogates. The results revealed that …
Sensitivity Of Planktonic Cells Of Staphylococcus Aureus To Elevated Hydrostatic Pressure As Affected By Mild Heat, Carvacrol, Nisin, And Caprylic Acid, Jyothi George, Sadiye Aras, Md Niamul Kabir, Sabrina Wadood, Shahid Chowdhury, Aliyar Cyrus Fouladkhah
Sensitivity Of Planktonic Cells Of Staphylococcus Aureus To Elevated Hydrostatic Pressure As Affected By Mild Heat, Carvacrol, Nisin, And Caprylic Acid, Jyothi George, Sadiye Aras, Md Niamul Kabir, Sabrina Wadood, Shahid Chowdhury, Aliyar Cyrus Fouladkhah
Agricultural and Environmental Sciences Faculty Research
Current study investigated effects of elevated hydrostatic pressure exposure in the presence of mild heat and natural antimicrobials against Staphylococcus aureus. Hydrostatic pressure of 350 to 550 MPa with nisin (5000 IU/mL), carvacrol, or caprylic acid (0.5% v/v) were applied for the reduction in four-strain mixture of S. aureus in HEPES buffer at 4 and 40 °C for up to 7 min. Results were statistically analyzed by ANOVA and D-values were additionally calculated using best-fitted linear model. Prior to exposure to treatments at 4 °C, counts of the pathogen were 7.95 ± 0.4 log CFU/mL and were reduced (p < 0.05) to 6.44 ± 0.3 log CFU/mL after 7 min of treatment at 450 MPa. D-value associated with this treatment was 5.34 min (R2 …
Integrate Structural Analysis, Isoform Diversity, And Interferon-Inductive Propensity Of Ace2 To Predict Sars-Cov2 Susceptibility In Vertebrates, Eric R. Sang, Yun Tian, Yuanying Gong, Laura C. Miller, Yongming Sang
Integrate Structural Analysis, Isoform Diversity, And Interferon-Inductive Propensity Of Ace2 To Predict Sars-Cov2 Susceptibility In Vertebrates, Eric R. Sang, Yun Tian, Yuanying Gong, Laura C. Miller, Yongming Sang
Agricultural and Environmental Sciences Faculty Research
The current new coronavirus disease (COVID-19) has caused globally over 0.4/6 million confirmed deaths/infected cases across more than 200 countries. As the etiological coronavirus (a.k.a. SARS-CoV2) may putatively have a bat origin, our understanding about its intermediate reservoir between bats and humans, especially its tropism in wild and domestic animals are mostly unknown. This constitutes major concerns in public health for the current pandemics and potential zoonosis. Previous reports using structural analysis of the viral spike protein (S) binding its cell receptor of angiotensin-converting enzyme 2 (ACE2), indicate a broad potential of SARS-CoV2 susceptibility in wild and particularly domestic animals. …
Permethrin Residual Activity Against Ambrosia Beetle (Coleoptera: Curculionidae: Scolytinae) Attacks Following Field Aging And Simulated Rainfall Weathering, Matthew S. Brown, Karla M. Addesso, Fulya Baysal-Gurel, Nadeer N. Youssef, Jason B. Oliver
Permethrin Residual Activity Against Ambrosia Beetle (Coleoptera: Curculionidae: Scolytinae) Attacks Following Field Aging And Simulated Rainfall Weathering, Matthew S. Brown, Karla M. Addesso, Fulya Baysal-Gurel, Nadeer N. Youssef, Jason B. Oliver
Agricultural and Environmental Sciences Faculty Research
Adult ambrosia beetles (Coleoptera: Curculionidae: Scolytinae) bore into ornamental nursery trees resulting in trunk vascular tissue damage, which can potentially kill trees. Ambrosia beetle exposure to surface-applied insecticides is minimal after internal trunk galleries are formed, so effective management requires insecticide treatments to be applied near the time of infestation or to have residual activity on the bark. Tree trunk sections (bolts) were used to determine the effect of field aging or irrigation (i.e., simulated rainfall weathering) on permethrin residual activity against ambrosia beetles. In all experiments, 30-cm-long bolts from Liriodendron tulipifera L. (Magnoliales: Magnoliaceae) were hollowed and filled with …
Prevalence Of Multidrug-Resistant Foodborne Pathogens And Indicator Bacteria From Edible Offal And Muscle Meats In Nashville, Tennessee, Siqin Liu, Agnes Kilonzo-Nthenge, Samuel N. Nahashon, Bharat Pokharel, Abdullah Ibn Mafiz, Maureen Nzomo
Prevalence Of Multidrug-Resistant Foodborne Pathogens And Indicator Bacteria From Edible Offal And Muscle Meats In Nashville, Tennessee, Siqin Liu, Agnes Kilonzo-Nthenge, Samuel N. Nahashon, Bharat Pokharel, Abdullah Ibn Mafiz, Maureen Nzomo
Agricultural and Environmental Sciences Faculty Research
This study investigated the prevalence of antimicrobial-resistant bacteria in retail edible offal and muscle meats in Nashville, Tennessee. A total of 348 retail meats (160 edible offal and 188 muscle) were analyzed for Salmonella enterica serovar, Campylobacter, Escherichia coli, E. coli O157:H7, and enterococci. Bacteria was identified using biochemical and PCR methods. Salmonella enterica serovar (4.4% and 4.3%), Campylobacter (1.9% and 1.1%), E. coli (79.4% and 89.4%), and enterococci (88.1% and 95.7%) was detected in offal and muscle meats, respectively. Chicken liver (9.7%) was most frequently contaminated with Salmonella enterica serovar, followed by ground chicken (6.9%) and chicken wings (4.2%). …
Pre-Emergence Herbicides And Mulches For Weed Control In Cutting Propagation, Anthony L. Witcher, Isha Poudel
Pre-Emergence Herbicides And Mulches For Weed Control In Cutting Propagation, Anthony L. Witcher, Isha Poudel
Agricultural and Environmental Sciences Faculty Research
Weed control is critical in cutting propagation to maximize root growth and liner quality, but hand weeding is time consuming and not cost efficient. Pre-emergence herbicides are widely used in container-grown nursery crop production, but concerns of potential phytotoxicity have limited widespread use in propagation. Mulches are a viable alternative for weed control but few products have been evaluated in propagation. Five pre-emergence herbicides and five mulches were evaluated for rooting stem cuttings of three nursery crop species and for control of four weed species. Mulches were applied (0.8 cm depth) prior to sticking cuttings while pre-emergence herbicides were applied …
Probiotics And Antimicrobial Effect Of Lactiplantibacillus Plantarum, Saccharomyces Cerevisiae, And Bifidobacterium Longum Against Common Foodborne Pathogens In Poultry, Joy Igbafe, Agnes Kilonzo-Nthenge, Samuel N. Nahashon, Abdullah Ibn Mafiz, Maureen Nzomo
Probiotics And Antimicrobial Effect Of Lactiplantibacillus Plantarum, Saccharomyces Cerevisiae, And Bifidobacterium Longum Against Common Foodborne Pathogens In Poultry, Joy Igbafe, Agnes Kilonzo-Nthenge, Samuel N. Nahashon, Abdullah Ibn Mafiz, Maureen Nzomo
Agricultural and Environmental Sciences Faculty Research
The probiotic potential and antimicrobial activity of Lactiplantibacillus plantarum, Saccharomyces cerevisiae, and Bifidobacterium longum were investigated against Escherichia coli O157:H7, Salmonella typhimurium and Listeria monocytogenes. Selected strains were subjected to different acid levels (pH 2.5–6.0) and bile concentrations (1.0–3.0%). Strains were also evaluated for their antimicrobial activity by agar spot test. The potential probiotic strains tolerated pH 3.5 and above without statistically significant growth reduction. However, at pH 2.5, a significant (p < 0.05) growth reduction occurred after 1 h for L. plantarum (4.32 log CFU/mL) and B. longum (5.71 log CFU/mL). S. cerevisiae maintained steady cell counts for the entire treatment period without a statistically significant (p > 0.05) reduction (0.39 log CFU/mL). The results indicate at 3% bile concertation, 1.86 log CFU/mL reduction was observed for L. plantarum, while S. cerevisiae, and B. longum growth …
Substrate Properties And Fertilizer Rates On Yield Responses Of Lettuce In A Vertical Growth System, Zadarreyal Wiggins, Onyekachukwu Akaeze, Dilip Nandwani, Anthony Witcher
Substrate Properties And Fertilizer Rates On Yield Responses Of Lettuce In A Vertical Growth System, Zadarreyal Wiggins, Onyekachukwu Akaeze, Dilip Nandwani, Anthony Witcher
Agricultural and Environmental Sciences Faculty Research
The increased demand for food and the challenge for space for agriculture production in urban centers have made the vertical growth system an interesting trend. Agriculture is no longer only the horizontal, traditional, and soil grown method. Urban agriculture has created ways for inner city growers to be able to farm in a restricted space. Vertical farming is the practice of growing crops, especially leafy vegetables such as lettuce, in vertically stacked layers, as this results in significantly higher plant population per unit area. Two research trials were conducted in the fall of 2018 and the spring of 2019 to …
Performance Of A Uv-A Led System For Degradation Of Aflatoxins B1 And M1 In Pure Water: Kinetics And Cytotoxicity Study, Judy Stanley, Ankit Patras, Brahmaiah Pendyala, Matthew J. Vergne, Rishipal R. Bansode
Performance Of A Uv-A Led System For Degradation Of Aflatoxins B1 And M1 In Pure Water: Kinetics And Cytotoxicity Study, Judy Stanley, Ankit Patras, Brahmaiah Pendyala, Matthew J. Vergne, Rishipal R. Bansode
Agricultural and Environmental Sciences Faculty Research
The efficacy of a UV-A light emitting diode system (LED) to reduce the concentrations of aflatoxin B1, aflatoxin M1 (AFB1, AFM1) in pure water was studied. This work investigates and reveals the kinetics and main mechanism(s) responsible for the destruction of aflatoxins in pure water and assesses the cytotoxicity in liver hepatocellular cells. Irradiation experiments were conducted using an LED system operating at 365 nm (monochromatic wave-length). Known concentrations of aflatoxins were spiked in water and irradiated at UV-A doses ranging from 0 to 1,200 mJ/cm2. The concentration of AFB1 and AFM1 was determined by HPLC with fluorescence detection. LC–MS/MS …
Transcriptome Analysis And Expression Of Selected Cationic Amino Acid Transporters In The Liver Of Broiler Chicken Fed Diets With Varying Concentrations Of Lysine, Collins N. Khwatenge, Boniface M. Kimathi, Samuel N. Nahashon
Transcriptome Analysis And Expression Of Selected Cationic Amino Acid Transporters In The Liver Of Broiler Chicken Fed Diets With Varying Concentrations Of Lysine, Collins N. Khwatenge, Boniface M. Kimathi, Samuel N. Nahashon
Agricultural and Environmental Sciences Faculty Research
Amino acids are known to play a key role in gene expression regulation. Amino acid signaling is mediated via two pathways: the mammalian target of rapamycin complex 1 (mTORC1) and the amino acid responsive (AAR) pathways. Cationic amino acid transporters (CATs) are crucial in these pathways due to their sensing, signaling and transport functions. The availability of certain amino acids plays a key role in the intake of other amino acids, hence affecting growth in young birds. However, the specific mechanism for regulating lysine transport for growth is not clear. In this study, we analyze the transcriptome profiles and mRNA …
Porcine Cytokines, Chemokines And Growth Factors: 2019 Update, Harry D. Dawson, Yongming Sang, Joan K. Lunney
Porcine Cytokines, Chemokines And Growth Factors: 2019 Update, Harry D. Dawson, Yongming Sang, Joan K. Lunney
Agricultural and Environmental Sciences Faculty Research
Pigs are a major food source worldwide as well as major biomedical models for human physiology and therapeutics. A thorough understanding of porcine immunity is essential to prevent and treat infectious diseases, and develop effective vaccines and therapeutics. The use of pigs as biomedical models is dependent on the growing molecular and immune toolbox. This paper summarizes current knowledge of swine cytokines, chemokines and growth factors, identifying 289 pig proteins, characterizing knowledge of their gene structures and families. It identifies areas in the current swine genome build that need to be clarified. A broad-based literature and vendor search was conducted …
Impact Of Winter Cover Crop Usage In Soilborne Disease Suppressiveness In Woody Ornamental Production System, Milan Panth, Fulya Baysal-Gurel, Terri Simmons, Karla M. Addesso, Anthony L. Witcher
Impact Of Winter Cover Crop Usage In Soilborne Disease Suppressiveness In Woody Ornamental Production System, Milan Panth, Fulya Baysal-Gurel, Terri Simmons, Karla M. Addesso, Anthony L. Witcher
Agricultural and Environmental Sciences Faculty Research
Diseases caused by soilborne pathogens are a major limitation to field grown nursery production. The application of cover crops for soilborne disease management has not been widely investigated in a woody ornamental nursery production system. The objective of this study was to explore the impact of winter cover crops usage on soilborne disease management in that system. Soils from established field plots of red maple (Acer rubrum L.) with and without winter cover crops (crimson clover (Trifolium incarnatum L.) or triticale (× Triticosecale W.)) were sampled following the senescence of the cover crops. Separate bioassays were performed using red maple …
Genome-Wide Snp Identification And Association Mapping For Seed Mineral Concentration In Mung Bean (Vigna Radiata L.), Xingbo Wu, A.S.M. Faridul Islam, Naransa Limpot, Lucas Mackasmiel, Jerzy Mierzwa, Andrés J. Cortés, Matthew W. Blair
Genome-Wide Snp Identification And Association Mapping For Seed Mineral Concentration In Mung Bean (Vigna Radiata L.), Xingbo Wu, A.S.M. Faridul Islam, Naransa Limpot, Lucas Mackasmiel, Jerzy Mierzwa, Andrés J. Cortés, Matthew W. Blair
Agricultural and Environmental Sciences Faculty Research
Mung bean (Vigna radiata L.) quality is dependent on seed chemical composition, which in turn determines the benefits of its consumption for human health and nutrition. While mung bean is rich in a range of nutritional components, such as protein, carbohydrates and vitamins, it remains less well studied than other legume crops in terms of micronutrients. In addition, mung bean genomics and genetic resources are relatively sparse. The objectives of this research were three-fold, namely: to develop a genome-wide marker system for mung bean based on genotyping by sequencing (GBS), to evaluate diversity of mung beans available to breeders …