Open Access. Powered by Scholars. Published by Universities.®
- Discipline
Articles 1 - 4 of 4
Full-Text Articles in Life Sciences
A Simple Method For Sphingolipid Analysis Of Tissues Embedded In Optimal Cutting Temperature Compound, Timothy Rohrbach, April E. Boyd, Pamela J. Grizzard, Sarah Speigel, Jeremy Allegood, Santiago Lima
A Simple Method For Sphingolipid Analysis Of Tissues Embedded In Optimal Cutting Temperature Compound, Timothy Rohrbach, April E. Boyd, Pamela J. Grizzard, Sarah Speigel, Jeremy Allegood, Santiago Lima
Richard Bland Faculty Works
MS-assisted lipidomic tissue analysis is a valuable tool to assess sphingolipid metabolism dysfunction in disease. These analyses can reveal potential pharmacological targets or direct mechanistic studies to better understand the molecular underpinnings and influence of sphingolipid metabolism alterations on disease etiology. But procuring sufficient human tissues for adequately powered studies can be challenging. Therefore, biorepositories, which hold large collections of cryopreserved human tissues, are an ideal retrospective source of specimens. However, this resource has been vastly underutilized by lipid biologists, as the components of OCT compound used in cryopreservation are incompatible with MS analyses. Here, we report results indicating that …
Fty720/Fingolimod Decreases Hepatic Steatosis And Expression Of Fatty Acid Synthase In Diet-Induced Nonalcoholic Fatty Liver Disease In Mice, Timothy D. Rohrbach, Amon Asgharpour, Melizza A. Maczis, David Montefusco, Ashley Cowart, Pierre Bedossa, Arun J. Sanyal, Sarah Spiegel
Fty720/Fingolimod Decreases Hepatic Steatosis And Expression Of Fatty Acid Synthase In Diet-Induced Nonalcoholic Fatty Liver Disease In Mice, Timothy D. Rohrbach, Amon Asgharpour, Melizza A. Maczis, David Montefusco, Ashley Cowart, Pierre Bedossa, Arun J. Sanyal, Sarah Spiegel
Richard Bland Faculty Works
Nonalcoholic fatty liver disease (NAFLD), a leading cause of liver dysfunction, is a metabolic disease that begins with steatosis. Sphingolipid metabolites, particularly ceramide and sphingosine-1-phosphate (S1P), have recently received attention for their potential roles in insulin resistance and hepatic steatosis. FTY720/fingolimod, a prodrug for the treatment of multiple sclerosis, is phosphorylated in vivo to its active phosphorylated form by sphingosine kinase 2 and has been shown to interfere with the actions of S1P and to inhibit ceramide biosynthesis. Therefore, in this study we investigated the effects of FTY720 in a diet-induced animal model of NAFLD (DIAMOND) that recapitulates the hallmarks …
Palatability And Chemical Defenses Of Sponges From The Western Antarctic Peninsula, Kevin Peters, Charles D. Amsler, James B. Mcclinotck, Rob W.M. Van Soest, Bill J. Baker
Palatability And Chemical Defenses Of Sponges From The Western Antarctic Peninsula, Kevin Peters, Charles D. Amsler, James B. Mcclinotck, Rob W.M. Van Soest, Bill J. Baker
Richard Bland Faculty Works
No abstract provided.
Comprehensive Evaluation Of The Palatability And Chemical Defenses Of Subtidal Macroalgae From The Antarctic Peninsula, Charles D. Amsler, Katrin Iken, James B. Mcclinotck, Margaret O. Amsler, Kevin Peters, Joanna M. Hubbard, F. Bruce Furrow, Bill J. Baker
Comprehensive Evaluation Of The Palatability And Chemical Defenses Of Subtidal Macroalgae From The Antarctic Peninsula, Charles D. Amsler, Katrin Iken, James B. Mcclinotck, Margaret O. Amsler, Kevin Peters, Joanna M. Hubbard, F. Bruce Furrow, Bill J. Baker
Richard Bland Faculty Works
The palatability of 35 non-encrusting, subtidal macroalgal species collected from the vicinity of Palmer Station, Antarctica (64°46’S, 64°03’W), was determined in laboratory bioassays utilizing sympatric sea stars and fish known to consume macroalgae in nature. Overall, 63% of the macroalgal species offered to sea stars and 83% of the macroalgal species offered to fish in thallus bioassays were significantly unpalatable. This included all of the ecologically dominant, overstory brown macroalgae in the region. When organic extracts of unpalatable macroalgal species were incorporated into artificial foods, 76% of the species unpalatable as thallus to sea stars were also unpalatable to them …