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Articles 1 - 5 of 5
Full-Text Articles in Life Sciences
Elucidating The Consequence And Cause Of Microrna Dysregulation In Amyotrophic Lateral Sclerosis (Als), Zachary C. E. Hawley
Elucidating The Consequence And Cause Of Microrna Dysregulation In Amyotrophic Lateral Sclerosis (Als), Zachary C. E. Hawley
Electronic Thesis and Dissertation Repository
Amyotrophic Lateral Sclerosis (ALS) is a progressive motor neurodegenerative disorder with an average life expectancy of 2-5 years post-diagnosis. Common pathological features associated with ALS are the formation of cytoplasmic inclusions of intermediate filaments and RNA-binding proteins within motor neurons. The formation of intermediate filament cytoplasmic inclusions is believed to be driven by a loss of stochiometric expression between five neuronal intermediate filament proteins—NFL, NFM, NFH, INA and PRPH—where there is a selective suppression of the steady-state levels of NEFL, INA and PRPH mRNA. Further, three RNA-binding proteins—TDP-43, FUS and RGNEF—have been shown to co-aggregate with each other in …
Microrna156: A Short Rna With A Major Role In Abiotic Stress Tolerance In Alfalfa, Biruk Ayenew Feyissa
Microrna156: A Short Rna With A Major Role In Abiotic Stress Tolerance In Alfalfa, Biruk Ayenew Feyissa
Electronic Thesis and Dissertation Repository
The highly conserved plant microRNA156, miR156, affects various aspects of plant development and stress response by silencing SQUAMOSA-PROMOTER BINDING PROTEIN-LIKE (SPL) transcription factors. Our understanding of the role of miR156 and its mode of action in alfalfa’s (Medicago sativa L.) response to drought and flooding is still elusive, and thus this study was aimed at filling this gap in knowledge. Physiological parameters, metabolite and transcriptional analyses showed an interplay between miR156/SPL13 and WD40-1/DFR to mitigate drought stress. Low to moderate levels of miR156 overexpression suppressed SPL13 and increased WD40-1 to fine-tune the DIHYDROFLAVONOL-4-REDUCTASE (DFR) level for …
The Role Of Mir-526b In Cox-2 Mediated Human Breast Cancer Progression And Induction Of Stem-Like Phenotype Via Ep4 Receptor Signaling, Erin O. Landman
The Role Of Mir-526b In Cox-2 Mediated Human Breast Cancer Progression And Induction Of Stem-Like Phenotype Via Ep4 Receptor Signaling, Erin O. Landman
Electronic Thesis and Dissertation Repository
Our laboratory previously established that aberrant expression of cyclo-oxygenase (COX)-2 promotes breast cancer progression and metastasis via multiple mechanisms, including stem-like cell (SLC) induction, owing to activation of the prostaglandin E2 receptor EP4. COX-2 expression was linked to up-regulation of miRNA-526b. We hypothesized that miR-526b is regulated by EP4 activity, and that miR-526b supports breast cancer progression and induction of SLCs. Using stably miR-526b transfected MCF-7 and SKBR-3 cells in functional assays, including tumorsphere formation in vitro and lung colony formation in vivo, we observed enhanced migration, invasion, proliferation, tumorsphere formation, and in vivo tumorigenecity compared to controls. EP4 …
Long-Term Genomic And Epigenomic Dysregulation As A Consequence Of Prenatal Alcohol Exposure: A Model For Fetal Alcohol Spectrum Disorders, Morgan L Kleiber, Eric J Diehl, Benjamin I Laufer, Katarzyna Mantha, Aniruddho Chokroborty-Hoque, Bonnie Alberry, Shiva M Singh
Long-Term Genomic And Epigenomic Dysregulation As A Consequence Of Prenatal Alcohol Exposure: A Model For Fetal Alcohol Spectrum Disorders, Morgan L Kleiber, Eric J Diehl, Benjamin I Laufer, Katarzyna Mantha, Aniruddho Chokroborty-Hoque, Bonnie Alberry, Shiva M Singh
Biology Publications
There is abundant evidence that prenatal alcohol exposure leads to a range of behavioral and cognitive impairments, categorized under the term fetal alcohol spectrum disorders (FASDs). These disorders are pervasive in Western cultures and represent the most common preventable source of neurodevelopmental disabilities. The genetic and epigenetic etiology of these phenotypes, including those factors that may maintain these phenotypes throughout the lifetime of an affected individual, has become a recent topic of investigation. This review integrates recent data that has progressed our understanding FASD as a continuum of molecular events, beginning with cellular stress response and ending with a long-term …
Reduced Expression Of Mir15a In The Blood Of Patients With Oral Squamous Cell Carcinoma Is Associated With Tumor Staging, Joao Artur Ricieri Brito, Carolina Cavalieri Gomes, Flavio Juliano Garcia Santos Pimenta, Alvimar Afonso Barbosa, Marco A. M. Prado, Vania F. Prado, Marcus Vinicius Gomez, Ricardo Santiago Gomez
Reduced Expression Of Mir15a In The Blood Of Patients With Oral Squamous Cell Carcinoma Is Associated With Tumor Staging, Joao Artur Ricieri Brito, Carolina Cavalieri Gomes, Flavio Juliano Garcia Santos Pimenta, Alvimar Afonso Barbosa, Marco A. M. Prado, Vania F. Prado, Marcus Vinicius Gomez, Ricardo Santiago Gomez
Anatomy and Cell Biology Publications
MicroRNAs (miRNAs) mirl 5a and let7a are iMportant regulators of bcl-2, ras and c-myc proteins Considering that these miRNAs are commonly altered in many human cancers and that these proteins are reported to be altered in oral squamous cell carcinoma (OSCC), we investigated them in a set of OSCC cases 'I he miRNAs as well as the proteins were evaluated in the tumor and blood of 20 patients by real-time quantitative PCR and iMmunohistochemistry, respectively The expression of nfirl5a and bcl-2 proteins in the tumors was not associated with each other or with tumor staging On the other hand, we …