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Saponin-Permeabilization Is Not A Viable Alternative To Isolated Mitochondria For Assessing Oxidative Metabolism In Hibernation, James Staples, Katherine E. Mathers
Saponin-Permeabilization Is Not A Viable Alternative To Isolated Mitochondria For Assessing Oxidative Metabolism In Hibernation, James Staples, Katherine E. Mathers
Biology Publications
Saponin permeabilization of tissue slices is increasingly popular for characterizing mitochondrial function largely because it is fast, easy, requires little tissue and leaves much of the cell intact. This technique is well described for mammalian muscle and brain, but not for liver. We sought to evaluate how saponin permeabilization reflects aspects of liver energy metabolism typically assessed in isolated mitochondria. We studied the ground squirrel (Ictidomys tridecemlineatus Mitchell), a hibernating mammal that shows profound and acute whole-animal metabolic suppression in the transition from winter euthermia to torpor. This reversible metabolic suppression is also reflected in the metabolism of isolated …
Saponin-Permeabilization Is Not A Viable Alternative To Isolated Mitochondria For Assessing Oxidative Metabolism In Hibernation, Katherine E. Mathers, James F. Staples
Saponin-Permeabilization Is Not A Viable Alternative To Isolated Mitochondria For Assessing Oxidative Metabolism In Hibernation, Katherine E. Mathers, James F. Staples
Biology Publications
Saponin permeabilization of tissue slices is increasingly popular for characterizing mitochondrial function largely because it is fast, easy, requires little tissue and leaves much of the cell intact. This technique is well described for mammalian muscle and brain, but not for liver. We sought to evaluate how saponin permeabilization reflects aspects of liver energy metabolism typically assessed in isolated mitochondria. We studied the ground squirrel (Ictidomys tridecemlineatus Mitchell), a hibernating mammal that shows profound and acute whole-animal metabolic suppression in the transition from winter euthermia to torpor. This reversible metabolic suppression is also reflected in the metabolism of isolated liver …