Life Sciences Commons^™

The Metabolic And Physiological Demands Of A Simulated Fire Ground Test Versus A Live-Fire Training Evolution In Professional Firefighters, Drew Edward Gonzalez, Hunter Waldman, Matthew J. Mcallister

International Journal of Exercise Science

International Journal of Exercise Science 16(7): 230-241, 2023. Objective: This study examined the similarities in metabolic and physiological demands of a fire ground test (FGT) and a live fire training evolution. Methods: Twenty-seven firefighters completed either a FGT (n = 13) or a live fire training evolution (n = 14). Salivary samples were collected pre, immediately post, and 30-minutes post FGT and live fire training evolution, and analyzed for cortisol, uric acid, and interleukin-1β (IL-1β). Heart rate (HR) was measured pre- and post-task. Results: Both tasks resulted in significant elevations in cortisol, IL-1β, and HR. Conclusions: Both the …

Effects Of Glucocorticoids Upon Pro-Inflammatory Responses To Acute Sleep Fragmentation, Hunter Weaver

Masters Theses & Specialist Projects

Sleep loss is a common problem in humans who suffer from obstructive sleep apnea. Sleep aids in the regulation of immune responses, some of which induce inflammatory responses. Cytokines regulate the inflammatory process and are released in response to sleep fragmentation (SF) in mice. Glucocorticoids are hormones that are released from the adrenal cortices during a stress response and are considered to be anti-inflammatory and immunosuppressive at high doses but may stimulate immune function on an acute level. The first hypothesis tested was that normal physiological expression of glucocorticoids (Sham) will display increased IL-1β and TNFα expression levels, while high …

Effect Of Acute Sleep Fragmentation Upon Inflammatory Response Of Brown And White Adipose Tissue In Male Mice, Zach Wriedt

Mahurin Honors College Capstone Experience/Thesis Projects

Sleep is an important process required for vertebrates, including humans, to function. When sleep is disrupted, it leads to deleterious effects such as inflammatory responses throughout the body. Past studies have shown that acute (24 h) sleep fragmentation (SF) leads to an inflammatory response in white adipose tissue. However, whether brown adipose tissue responds in a similar fashion is unknown. Male adult (>8 weeks of age) C57BL/6j mice were subjected to SF for 24 h using a cage outfitted with a bar that moves horizontally across the cage every 2 min to periodically awaken mice (N =10). Controls were …

The Effect Of Age On Neurological Inflammation To Acute Sleep Fragmentation In Mice, Molly Taylor

Mahurin Honors College Capstone Experience/Thesis Projects

Obstructive sleep apnea is identified by recurring events of airway collapse during sleep, intermittent hypoxia, and perturbations in sleep continuity, known as sleep fragmentation. There is evidence to suggest that elderly patients are more at risk of developing obstructive sleep apnea. The purpose of this study was to assess whether age affects neurological inflammatory responses to acute sleep fragmentation. This assessment was made by subjecting young (4-5 months old) and old (10-11 months old) male C57BL/6j mice to automated sleep fragmentation, as well as having mice in both age categories as a control with no sleep fragmentation, for twenty-four hours. …

Effects Of Alpha- And Beta-Adrenergic Receptor Blockade Upon Inflammatory Responses To Acute And Chronic Sleep Fragmentation, Nicholas David Wheeler

Masters Theses & Specialist Projects

Generally, sleep is viewed as a recuperative process and its dysregulation has

cognitive, metabolic, immunological, and inflammatory implications that are largely deleterious to human health. Epidemiological and empirical studies have suggested that sleep fragmentation (SF) as result of obstructive sleep apnea (OSA) and other sleep abnormalities leads to pronounced systemic inflammatory responses, which are influenced by the sympathetic nervous system (SNS). However, the underlying molecular mechanisms contributing to SNS regulation of SF-induced inflammatory states are not fully understood. To assess the effects of the SNS system, C57BL/6j female mice were placed in automated SF chambers (12L:12D) and subjected to either …

Serum Tumor Necrosis Factor-Alpha Associates With Myocardial Oxygen Demand And Exercise Tolerance In Postmenopausal Women, Stephen J. Carter, David R. Bryan, William H. Neumeier, Stephen P. Glasser, Gary R. Hunter

International Journal of Exercise Science

International Journal of Exercise Science 11(2): 42-54, 2018. The functional implications of serum tumor necrosis factor-alpha (TNF-a), a marker of oxidative stress, on hemodynamic parameters at rest and during physical exertion are unclear. The aims of this investigation were to examine the independent associations of TNF-a on myocardial oxygen demand at rest and during submaximal exercise, while also evaluating the association of TNF-a on exercise tolerance. Forty, postmenopausal women, provided blood samples and completed a modified-Balke protocol to measure maximal oxygen uptake (VO_2max). Large artery compliance was measured by pulse contour analyses while rate-pressure product (RPP), an index …

Using Dry Blood Spots To Evaluate Serum Cytokines And Chemokines In Humans Via Multiplex Technology, Eric A. Prado, Adam S. Venable, Andie L. Henning, Randall R. Williams, Brian K. Mcfarlin

International Journal of Exercise Science: Conference Proceedings

Introduction: Dried Blood Spot (DBS) analysis has been used routinely since 1963 for the assessment of metabolic diseases in neonates; however, recent efforts have focused on the refinement and validation of DBS for other patient populations. The purpose of this study was to adapt existing DBS methods to analyze 38 serum cytokines/chemokines in human subjects. Validation will be completed by comparing DBS to serum for a given analyte.

Methods: After providing informed consent, subjects (N=21) provided a finger-stick DBS or venous serum sample using standard technique. Subjects were apparently healthy, non-obese, and had no known disease. Finger-stick capillary blood samples …

Changes Of Cytokines In Mouse Skeletal Muscle And Plasma By A Single Bout Of Endurance Exercise., Minjung Lee, Noriaki Kawanishi, Koichi Yada, Yungli Hung, Tsubasa Mizokami, Hiroyuki Niihara, Katsuhiko Suzuki

International Journal of Exercise Science: Conference Proceedings

Introduction: Exercise influences some cytokine levels, which is considered the body’s reaction against endogenous stress. Previous human studies demonstrated that high intensity exercise contributes to a dramatic increase of plasma cytokines like Interleukin-6 (IL-6) and Interleukin-1 receptor antagonist (IL-1ra) immediately after exercise. Recently, it has been identified that skeletal muscle produces cytokines and peptides and they exert both paracrine and endocrine effects. However, the sequential changes of cytokine production according to exercise conditions such as intensity, types and working time are still unclear. In this study, we identified the sequential changes of cytokines in both skeletal muscle and plasma after …

Circuit Resistance Training In Women: Body Composition And Serum Cytokines Levels, Fabiano Candido Ferreira, Alexandra Medeiros, Cristiane Nicioli, João Nunes

International Journal of Exercise Science: Conference Proceedings

Exercise can generate adjustments on body composition and elicit benefits or downregulation on the immunologic system. The aim of this study was to analyze the effects of circuit resistance training (CRT) on fat free mass (FFM), fat mass (FM) and cytokine responses in sedentary women, 39.71 ± 3.8 years old (n=14). The protocol consisted of 3 sessions/week of a circuit training of 9 stations with alternating muscle groups. In each session, the subjects performed the circuit 2 times with one set of 8-12 maximal repetitions (RM) in each station, during 10 weeks. The body composition was analyzed by DXA and, …

Inflammatory, Hematological And Oxidative Stress Responses In 160 To 246 Km Ultramarathons, Taisa Belli, Claudio Alexandre Gobatto

International Journal of Exercise Science: Conference Proceedings

Every race with distance higher than marathon is called ultramarathon. There are short ultramarathons, with a distance of 50 to 100km, such as the "Comrade Marathon" in South Africa (89km). On the other hand there are the long ultramarathons ranging from 100 to 1600km. Among these we can highlight the "Western States Endurance Run" (160km) in California/USA; the "Badwater Ultramarathon" (216 km) in California/USA and the "Spartahlon Ultramarathon" (246 km) in Greece. This literature review aimed to verify the inflammatory, hematological and oxidative stress responses particularly induced by these ultramarathons ranging from 160 to 246 km. These races present long …

Effect Of Eleven Weeks Training Program On Inflammatory, Hematological And Biochemical Parameters In Rat Skeletal Muscle, Liver And Serum, Fernando Oliveira Catanho Da Silva, Rejane Lucco, Rodrigo Ferraresso, Danilo Lopes Ferrucci

International Journal of Exercise Science: Conference Proceedings

Physical exercise is known causer of acute traumas in biological structures. Chronically, physical training or can lead to a coherent sequence of traumas leading to Adaptation (Functional Overreaching – FOR) or to an imbalance between stimulus and recovery leading to Overtraining Syndrome (OTS). In the present study we analyzed muscle and hepatic cytokines concentration: Interleukin-6 (IL6), Tumor Necrose Factor-Alpha (TNF-α), Interleukin-1Beta (IL1-β) and Interleukin-10 (IL-10); muscle and serum concentration of glutamate (GLU) and glutamine (GLN); serum concentration of C-reactive Protein (CRP), Alpha-1 Glicoprotein Acid (AGA), Albumin (ALB), Uric Acid (UA), Ferric Reducing Ability of Plasma (FRAP), Creatine Kinase (CK), Red …

Acute Effects Of A Short-Term And High Intensity Exercise On Serum Cytokines Concentrations Of Sedentary Women Submitted To A Circuit Resistance Training, Cristiane Nicioli, João Elias Nunes, Gabriela Bombarda, Fabiano Candido Ferreira

International Journal of Exercise Science: Conference Proceedings

The aim of this study was to evaluate the acute effects of short-term and high intensity exercise on serum cytokines concentrations, oxygen consumption and power of sedentary women pre and post 10 weeks of circuit resistance training (CRT). 14 women, 40.23 ± 3.9 years old, 164 ± 6.6 cm and 57.84 ± 7.7 kg were evaluated. Ventilatory threshold (WVT) and VO2peak (IVO2peak) were determined by ergoespirometric incremental test in a cycloergometer. In pre and post CRT, the women performed a test of cycling exercise with two stages: pedaling 30 minutes on WVT and 5 minutes on IVO2peak. The VO2peak was …