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Full-Text Articles in Life Sciences

Optimization Of Engineered Super Donor Strains Of Cryphonectria Parasitica To Reduce Canker Expansion In A Forest Setting, Amy Michelle Metheny Jan 2019

Optimization Of Engineered Super Donor Strains Of Cryphonectria Parasitica To Reduce Canker Expansion In A Forest Setting, Amy Michelle Metheny

Graduate Theses, Dissertations, and Problem Reports

Chestnut blight, caused by the non-native fungus Cryphonectria parasitica, has decimated American chestnut (Castanea dentata) populations throughout the eastern U.S. over the last century. Biological control of blight, termed hypovirulence, has emerged naturally in the form of a debilitating hypovirus transmitted through hyphal anastomosis among vegetatively compatible C. parasitica strains. Six unlinked vegetative incompatibility (vic) loci regulate hyphal anastomosis and thus, hypovirus transmission, which has limited biocontrol efficacy in genotypically diverse communities. Recently, four of six vic loci have been disrupted to engineer two “super donor” or SD strains. In combining these strains, enhanced transmission …


Elucidating Disease Dynamics In The Biocontrol Of Ailanthus Altissima While Confirming The Host Specificity Of The Vascular Wilt Pathogen Verticillium Nonalfalfae, Kristen L. Wickert Jan 2019

Elucidating Disease Dynamics In The Biocontrol Of Ailanthus Altissima While Confirming The Host Specificity Of The Vascular Wilt Pathogen Verticillium Nonalfalfae, Kristen L. Wickert

Graduate Theses, Dissertations, and Problem Reports

Ailanthus altissima is a highly invasive exotic tree species entrenched throughout the United States. Since the discovery of a vascular wilt disease of Ailanthus in 2002, caused by the fungus Verticillium nonalfalfae, many studies are investigating its potential as a biocontrol focusing on efficacy, host specificity and disease transmission. This study addresses: the reproductive potential of Ailanthus; if V. dahliae alters Verticillium wilt progression; if hardiness zones influence the individual contributions of two Verticillium spp.; can a unique habitat affect biocontrol efficacy; and can the fungal proteome of V. nonalfalfae allow us to explain differences in host …