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Full-Text Articles in Life Sciences

A Spatially Distributed Investigation Of Stream Water Temperature In A Contemporary Mixed-Land-Use Watershed, Jason P. Horne, Jason A. Hubbart Jul 2020

A Spatially Distributed Investigation Of Stream Water Temperature In A Contemporary Mixed-Land-Use Watershed, Jason P. Horne, Jason A. Hubbart

Faculty & Staff Scholarship

Stream water temperature (◦C) is an important physical variable that influences many biological and abiotic water quality processes. The intermingled mosaic of land-use/land-cover (LULC) types and corresponding variability in stream water temperature (Tw) processes in contemporary mixed-land-use watersheds necessitate research to advance management and policy decisions. Water temperature was analyzed from 21 gauging sites using a nested-scale experimental watershed study design. Results showed that forested land use was negatively correlated (α = 0.05) with mean and maximum Tw. Agricultural land use was significantly positively correlated (α = 0.05) with maximum Tw except during the spring season. Mixed development and Tw …


Treatment With Calcium Chloride Enhances Water Deficit Stress Tolerance In Viola (Viola Cornuta), Suejin Park, Youyoun Moon, Nicole L. Waterland Jan 2020

Treatment With Calcium Chloride Enhances Water Deficit Stress Tolerance In Viola (Viola Cornuta), Suejin Park, Youyoun Moon, Nicole L. Waterland

Faculty & Staff Scholarship

Water deficit stress can reduce the postproduction shelf life and marketability of floriculture crops. To alleviate the damage by water deficiency, plants need to limit transpirational water loss by inducing stomatal closure. Osmotic stress induces stomatal closure like the response to water deficit stress. It could be used as a convenient tool to enhance water deficit stress tolerance by reducing water loss. The objective of this research was to investigate whether osmotic treatment with a high concentration of chemical solutions could trigger a response to osmotic stress so that stomatal closure can be induced, resulting in enhanced water deficit stress …


The Rise To Dominance Of Genetic Model Organisms And The Decline Of Curiosity-Driven Organismal Research, Sarah M. Farris Jan 2020

The Rise To Dominance Of Genetic Model Organisms And The Decline Of Curiosity-Driven Organismal Research, Sarah M. Farris

Faculty & Staff Scholarship

Curiosity-driven, basic biological research “…performed without thought of practical ends…” establishes fundamental conceptual frameworks for future technological and medical breakthroughs. Traditionally, curiosity-driven research in biological sciences has utilized experimental organisms chosen for their tractability and suitability for studying the question of interest. This approach leverages the diversity of life to uncover working solutions (adaptations) to problems encountered by living things, and evolutionary context as to the extent to which these solutions may be generalized to other species. Despite the well-documented success of this approach, funding portfolios of United States granting agencies are increasingly filled with studies on a few species …


What Might It Cost To Increase Soil Organic Carbon Using No-Till On U.S. Cropland?, Mark Sperow Jan 2020

What Might It Cost To Increase Soil Organic Carbon Using No-Till On U.S. Cropland?, Mark Sperow

Faculty & Staff Scholarship

Existing research provides estimates of the biophysical potential for increasing soil organic carbon (SOC) stock, however additional research is needed to enhance our understanding of the economic potential for agricultural soils to offset or help reduce CO2emissions. This study derives the marginal cost to increase SOC sequestration by combining SOC sequestration potential estimates developed using the Intergovernmental Panel on Climate Change (IPCC) factors with an existing payment scheme that was designed to increase no-till (NT) adoption on U.S. cropland. The marginal costs of increasing SOC is a function of the amount of SOC that could be increased through NT and …


Experiences Of Multidisciplinary Health Professionals From A Culinary Medicine Cultural Immersion: Qualitative Analysis, Melissa D. Olfert, Rachel A. Wattick, Rebecca L. Hagedorn Jan 2020

Experiences Of Multidisciplinary Health Professionals From A Culinary Medicine Cultural Immersion: Qualitative Analysis, Melissa D. Olfert, Rachel A. Wattick, Rebecca L. Hagedorn

Faculty & Staff Scholarship

Purpose: Increasing Culinary Health Opportunities for Professionals (iCHOP) aimed to educate future and current health pro- fessionals on nutrition as medicine. Methods: Two cohorts each participated in a 16-week course followed by a cultural immersion in Tuscany, Italy. The course took place online through West Virginia University. After the course, participants traveled to Tuscany, Italy for a 2-week cultural im- mersion. The online course covered culinary medicine, the Mediterranean Diet, and cultural comparisons. The cultural immersion consisted of hands-on activities including culinary lessons, food production and organic farm tours, and tastings of Mediterranean foods. Data was collected through personal journaling …


Eb4cast Approach Improves Science Communication With Stakeholders In A College-Based Health Program, Melissa D. Olfert, Makenzie L. Barr, Rebecca L. Hagedorn, Rachel A. Wattick, Wenjun Zhou, Tanya M. Horacek, Anne E. Mathews, Kendra K. Kattelmann, Tandalayo Kidd, Adrienne A. White, Onikia N. Brown, Jesse Stabile Morrell, Lisa Franzen-Castle, Karla P. Shelnutt, Carol Byrd-Bredbenner, Terezie Tolar-Peterson, Geoffrey W. Greene, Sarah E. Colby Jan 2020

Eb4cast Approach Improves Science Communication With Stakeholders In A College-Based Health Program, Melissa D. Olfert, Makenzie L. Barr, Rebecca L. Hagedorn, Rachel A. Wattick, Wenjun Zhou, Tanya M. Horacek, Anne E. Mathews, Kendra K. Kattelmann, Tandalayo Kidd, Adrienne A. White, Onikia N. Brown, Jesse Stabile Morrell, Lisa Franzen-Castle, Karla P. Shelnutt, Carol Byrd-Bredbenner, Terezie Tolar-Peterson, Geoffrey W. Greene, Sarah E. Colby

Faculty & Staff Scholarship

Communicating scientific results with community partners is often lacking in intervention programs, thus eB4CAST was developed to facilitate impact sharing. This article investigated using the eB4CAST dissemination tool to communicate impact from a campus-based obesity prevention program. Data from Get Fruved RCT university sites collected at baseline were used to generate eB4CAST reports. Experts (n = 13) and RCT sites (n = 15) were asked to provide feedback on eB4CAST reports based on appeal, understanding, and clarity. On all Likert items, participants rated above 7 on each (out of 10). Positive responses from open-ended questions included eB4CAST reports being clear, …


Mechanism Of Plant Growth Promotion And Disease Suppression By Chitosan Biopolymer, Moutoshi Chakraborty, Mirza Hasanuzzaman, Mahfuzur Rahman, Md. Arifur Rahman Khan, Pankaj Bhowmik, Nur Uddin Mahmud, Mohsin Tanveer, Tofazzal Islam Jan 2020

Mechanism Of Plant Growth Promotion And Disease Suppression By Chitosan Biopolymer, Moutoshi Chakraborty, Mirza Hasanuzzaman, Mahfuzur Rahman, Md. Arifur Rahman Khan, Pankaj Bhowmik, Nur Uddin Mahmud, Mohsin Tanveer, Tofazzal Islam

Faculty & Staff Scholarship

The chitosan (CHT) biopolymer is a de-acetylated chitin derivative that exists in the outer shell of shrimp, shellfish, lobster or crabs, as well as fungal cell walls. Because of its biodegradability, environmental non-toxicity, and biocompatibility, it is an ideal resource for sustainable agriculture. The CHT emerged as a promising agent used as a plant growth promoter and also as an antimicrobial agent. It induces plant growth by influencing plant physiological processes like nutrient uptake, cell division, cell elongation, enzymatic activation and synthesis of protein that can eventually lead to increased yield. It also acts as a catalyst to inhibit the …


Wheat Blast: A New Threat To Food Security, M. Tofazzal Islam, Dipali Rani Gupta, Akbar Hossain, Krishna K. Roy, Xinyao He, Muhammad R. Kabir, Pawan K. Singh, Md. Arifur Rahman Khan, Mahfuzur Rahman, Guo-Liang Wang Jan 2020

Wheat Blast: A New Threat To Food Security, M. Tofazzal Islam, Dipali Rani Gupta, Akbar Hossain, Krishna K. Roy, Xinyao He, Muhammad R. Kabir, Pawan K. Singh, Md. Arifur Rahman Khan, Mahfuzur Rahman, Guo-Liang Wang

Faculty & Staff Scholarship

Wheat blast, caused by the Magnaporthe oryzae Triticum (MoT) lineage (synonym Pyricularia oryzae Triticum lineage), is a destructive disease in South America and Bangladesh. It is primarily a disease of wheat head, which can cause yield loss up to 100% under favorable disease conditions. The head infection results in complete or partial bleaching of the spike above the point of infection with either no grain or shriveled grain with low test weight. Due to low fungicide efficacy against the disease and lack of availability of resistant varieties, an integrated management program should be adopted to control this serious wheat disease. …


Suitable Methods For Isolation, Culture, Storage And Identification Of Wheat Blast Fungus Magnaporthe Oryzae Triticum Pathotype, Dipali Rani Gupta, Musrat Zahan Surovy, Nur Uddin Mahmud, Moutoshi Chakraborty, Sanjoy Kumar Paul, Md. Shaid Hossain, Pallab Bhattacharjee, Md. Shabab Mehebub, Kanistha Rani, Rumana Yeasmin, Mahfuzur Rahman, Md Tofazzal Islam Jan 2020

Suitable Methods For Isolation, Culture, Storage And Identification Of Wheat Blast Fungus Magnaporthe Oryzae Triticum Pathotype, Dipali Rani Gupta, Musrat Zahan Surovy, Nur Uddin Mahmud, Moutoshi Chakraborty, Sanjoy Kumar Paul, Md. Shaid Hossain, Pallab Bhattacharjee, Md. Shabab Mehebub, Kanistha Rani, Rumana Yeasmin, Mahfuzur Rahman, Md Tofazzal Islam

Faculty & Staff Scholarship

Wheat blast disease caused by a South American lineage of Magnaporthe oryzae Triticum (MoT) pathotype has emerged as a serious threat to wheat production in Bangladesh since its first emergence in 2016. Efficient and suitable methods for isolation, storage, inoculum production and molecular characterization of the pathogen can help in achieving the target of sustainable management of the disease in a relatively short period of time. In this study, we aimed to develop suitable methods for isolation, storage and morphological characterization and molecular identification of MoT isolates collected from the blast-infected wheat fields in Bangladesh. This process included modification of …


Vulnerability Of High-Elevation Endemic Salamanders To Climate Change: A Case Study With The Cow Knob Salamander (Plethodon Punctatus), Carl D. Jacobsen, Donald J. Brown, William D. Flint, Thomas K. Pauley, Kurt A. Buhlmann, Joseph C. Mitchell Jan 2020

Vulnerability Of High-Elevation Endemic Salamanders To Climate Change: A Case Study With The Cow Knob Salamander (Plethodon Punctatus), Carl D. Jacobsen, Donald J. Brown, William D. Flint, Thomas K. Pauley, Kurt A. Buhlmann, Joseph C. Mitchell

Faculty & Staff Scholarship

Rapid contemporary climate change is a potential threat to long-term persistence of montane wildlife species because they often have narrow thermal tolerances and have limited potential to shift their distributions. The Appalachian Mountain region in the eastern United States is a global biodiversity hotspot for woodland salamanders (genus Plethodon), many of which are high-elevation endemic species. Robust assessments of the vulnerability of high-elevation endemic salamanders to climate change, including delineation of future potential climate refugia, are needed to guide climate change adaptations strategies. The Cow Knob Salamander (Plethodon punctatus) is a species of conservation concern found at …


A Comparison Of Wetland Characteristics Between Agricultural Conservation Easement Program And Public Lands Wetlands In West Virginia, Usa, Katherine E. Lewis, Christopher T. Rota, James T. Anderson Jan 2020

A Comparison Of Wetland Characteristics Between Agricultural Conservation Easement Program And Public Lands Wetlands In West Virginia, Usa, Katherine E. Lewis, Christopher T. Rota, James T. Anderson

Faculty & Staff Scholarship

In West Virginia, USA, there are 24 conservation easement program wetlands enrolled in the Agricultural Conservation Easement Program (ACEP). These wetlands are located on private agricultural land and are passively managed. Due to their location within fragmented agricultural areas, wetlands enrolled in ACEP in West Virginia have the potential to add wetland ecosystem services in areas that are lacking these features. We evaluated ACEP wetlands compared to reference wetlands on public land in West Virginia by using surrounding land cover, vegetative cover, and wetland features and stressors such as the presence or absence of erosion, upland inclusion, algal mats, and …


Saccharomyces Arboricola And Its Hybrids’ Propensity For Sake Production: Interspecific Hybrids Reveal Increased Fermentation Abilities And A Mosaic Metabolic Profile, Matthew J. Winans, Yuki Yamamoto, Yuki Fujimaru, Yuki Kusaba, Jennifer E G Gallagher, Hiroshi Kitagaki Jan 2020

Saccharomyces Arboricola And Its Hybrids’ Propensity For Sake Production: Interspecific Hybrids Reveal Increased Fermentation Abilities And A Mosaic Metabolic Profile, Matthew J. Winans, Yuki Yamamoto, Yuki Fujimaru, Yuki Kusaba, Jennifer E G Gallagher, Hiroshi Kitagaki

Faculty & Staff Scholarship

The use of interspecific hybrids during the industrial fermentation process has been well established, positioning the frontier of advancement in brewing to capitalize on the potential of Saccharomyces hybridization. Interspecific yeast hybrids used in modern monoculture inoculations benefit from a wide range of volatile metabolites that broaden the organoleptic complexity. This is the first report of sake brewing by Saccharomyces arboricola and its hybrids. S. arboricola x S. cerevisiae direct-mating generated cryotolerant interspecific hybrids which increased yields of ethanol and ethyl hexanoate compared to parental strains, important flavor attributes of fine Japanese ginjo sake rice wine. Hierarchical clustering heatmapping with …


Oxidative Stress Responses And Nutrient Starvation In Mchm Treated Saccharomyces Cerevisiae, Michael C. Ayers, Zachary N. Sherman, Jennifer E. G. Gallagher Jan 2020

Oxidative Stress Responses And Nutrient Starvation In Mchm Treated Saccharomyces Cerevisiae, Michael C. Ayers, Zachary N. Sherman, Jennifer E. G. Gallagher

Faculty & Staff Scholarship

In 2014, the coal cleaning chemical 4-methylcyclohexane methanol (MCHM) spilled into the water supply for 300,000 West Virginians. Initial toxicology tests showed relatively mild results, but the underlying effects on cellular biology were underexplored. Treated wildtype yeast cells grew poorly, but there was only a small decrease in cell viability. Cell cycle analysis revealed an absence of cells in S phase within thirty minutes of treatment. Cells accumulated in G1 over a six-hour time course, indicating arrest instead of death. A genetic screen of the haploid knockout collection revealed 329 high confidence genes required for optimal growth in MCHM. These …


Blood Substitution Therapy Rescues The Brain Of Mice From Ischemic Damage, Xuefang Ren, Heng Hu, Imran Farooqi, James W. Simpkins Jan 2020

Blood Substitution Therapy Rescues The Brain Of Mice From Ischemic Damage, Xuefang Ren, Heng Hu, Imran Farooqi, James W. Simpkins

Faculty & Staff Scholarship

Acute stroke causes complex, pathological, and systemic responses that have not been treatable by any single medication. In this study, using a murine transient middle cerebral artery occlusion stroke model, a novel therapeutic strategy is proposed, where blood replacement (BR) robustly reduces infarctions and improves neurological deficits in mice. Our analyses of immune cell subsets suggest that BR therapy substantially decreases neutrophils in blood following a stroke. Electrochemiluminescence detection demonstrates that BR therapy reduces cytokine storm in plasma and ELISA demonstrates reduced levels of matrix metalloproteinase-9 (MMP-9) in the plasma and brains at different time points post-stroke. Further, we have …