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Full-Text Articles in Life Sciences
Cathepsin B: A Potential Prognostic Marker For Inflammatory Breast Cancer., Nouh A. Mohamed, Mona M. Mohamed, Mohamed El-Shinawi, Mohamed A. Shaalan, Dora Cavallo-Medved, Hussein M. Khaled, Bonnie F. Sloane
Cathepsin B: A Potential Prognostic Marker For Inflammatory Breast Cancer., Nouh A. Mohamed, Mona M. Mohamed, Mohamed El-Shinawi, Mohamed A. Shaalan, Dora Cavallo-Medved, Hussein M. Khaled, Bonnie F. Sloane
Biological Sciences Publications
Inflammatory breast cancer (IBC) is the most aggressive form of breast cancer. In non-IBC, the cysteine protease cathepsin B (CTSB) is known to be involved in cancer progression and invasion; however, very little is known about its role in IBC. In this study, we enrolled 23 IBC and 27 non-IBC patients. All patient tissues used for analysis were from untreated patients. Using immunohistochemistry and immunoblotting, we assessed the levels of expression of CTSB in IBC versus non-IBC patient tissues. Previously, we found that CTSB is localized to caveolar membrane microdomains in cancer cell lines including IBC, and therefore, we also …
Interleukin-6 Increases Expression And Secretion Of Cathepsin B By Breast Tumor-Associated Monocytes., Mona M. Mohamed, Dora Cavallo-Medved, Deborah Rudy, Arulselvi Anbalagan, Bonnie F. Sloane, Kamir Moin
Interleukin-6 Increases Expression And Secretion Of Cathepsin B By Breast Tumor-Associated Monocytes., Mona M. Mohamed, Dora Cavallo-Medved, Deborah Rudy, Arulselvi Anbalagan, Bonnie F. Sloane, Kamir Moin
Biological Sciences Publications
In the tumor microenvironment, monocytes respond to paracrine stimuli from breast cancer cells by secreting molecules that participate in breast cancer growth, invasion, intravasation and metastasis. Here we examined the effects of media conditioned by MDA-MB-231 human breast carcinoma cells (231-CM) on expression and secretion of proteases and secretion of cytokines by U937 human monocytes. We found that 231-CM increased U937: 1) proliferation; 2) expression, activity and secretion of the cysteine protease cathepsin B (CTSB); 3) secretion of matrix metalloproteinases (MMP)-2 and -9; and 4) secretion of interleukin-6 (IL-6) and insulin-like growth factor binding protein-1 (IGFBP-1). We further demonstrated by …
Functional Live-Cell Imaging Demonstrates That Beta1-Integrin Promotes Type Iv Collagen Degradation By Breast And Prostate Cancer Cells., Mansoureh Sameni, Julie Dosescu, Kenneth M. Yamada, Dora Cavallo-Medved, Bonnie F. Sloane
Functional Live-Cell Imaging Demonstrates That Beta1-Integrin Promotes Type Iv Collagen Degradation By Breast And Prostate Cancer Cells., Mansoureh Sameni, Julie Dosescu, Kenneth M. Yamada, Dora Cavallo-Medved, Bonnie F. Sloane
Biological Sciences Publications
The ability of tumor cells to adhere to, migrate on, and remodel extracellular matrices is mediated by cell surface receptors such as beta1-integrins. Here we conducted functional live-cell imaging in real time to investigate the effects of modulating beta1-integrin expression and function on proteolytic remodeling of the extracellular matrix. Human breast and prostate cancer cells were grown on reconstituted basement membrane containing a quenched fluorescent form of collagen IV. Generation of cleavage products and the resulting increases in fluorescence were imaged and quantified. Decreases in the expression and activity of beta1-integrin reduced digestion of quenched fluorescent-collagen IV by the breast …
Caveolin-1-Mediated Expression And Secretion Of Kallikrein 6 In Colon Cancer Cells., Rebecca S. Henkhaus, Upal Kunal Basu Roy, Dora Cavallo-Medved, Bonnie F. Sloane
Caveolin-1-Mediated Expression And Secretion Of Kallikrein 6 In Colon Cancer Cells., Rebecca S. Henkhaus, Upal Kunal Basu Roy, Dora Cavallo-Medved, Bonnie F. Sloane
Biological Sciences Publications
Kallikreins are secreted proteases that may play a functional role and/or serve as a serum biomarker for the presence or progression of certain types of cancers. Kallikrein 6 (KLK6) has been shown to be upregulated in several types of cancers, including colon. The aims of this study were to elucidate pathways that influence KLK6 gene expression and KLK6 protein secretion in the HCT116 human colon cancer cells. Our data indicate a central role for caveolin-1 (CAV-1), the main structural protein of caveolae, in both KLK6 gene expression and protein secretion. Sucrose gradient subcellular fractionation reveals that CAV-1 and KLK6 colocalize …