Life Sciences Commons^™

Morfología Y Moléculas: Bases Complementarias De La Sistemática Moderna, Rafael O. De Sá, David M. Hillis

Biology Faculty Publications

RESUMEN

El auge de las técnicas moleculares en las últimas dos décadas ha traído nuevos enfoques en los estudios sistemáticos. Aquí presentaremos una revisión del uso, las aplicaciones, y los problemas de las principales metodologías de trabajo. Cada una de estas técnicas es aplicable a algunos problemas e inapropiada para otros, y es importante entender las suposiciones y limitaciones de cada técnica. Concluimos que el uso combinado de los enfoques morfológicos y moleculares otorga mejor resolución a los problemas sistemáticos.

ABSTRACT

The development of molecular techniques during the last two decades has brought new approaches to the analysis of systematic …

A Survey Of Mucilaginous Testa In Chamaesyce, Martha S. Jordan, W. John Hayden

Biology Faculty Publications

Seeds of Chamaesyce were studied for presence of mucilaginous testa. Samples were selected to represent all major systematic sections within the genus. Observations were made with a dissecting microscope following brief hydration of seeds; additional SEM observations of both intact and fractured dry seeds were made for selected species. With few exceptions, most of Boissier's subsections test positively for seed mucilage; however, mucilage is generally absent in subsections Gymnadeniae and "Sclerophyllae", for which absence of mucilage is regarded as secondary loss from mucilagionus ancestors. Although mucilage production is associated with a well-defined subepidermal layer of macroesclereids, it is …

Notes On Neotropical Amanoa (Euphorbiaceae), W. John Hayden

Biology Faculty Publications

Lectotypes are designated for Amanoa caribaea Krug & Urban and A. guianensis Aublet; presumed syntypes of the latter taxon are shown to be heterogeneous by inclusion of a previously unrecognized species. Four new species of Amanoa are described: A. congesta from French Guiana and northeastern Brazil; A. gracillima from Manaus, Brazil; A. nanayensis from Amazonian Peru and adjacent Colombia and Brazil; and A. neglecta from French Guiana and Surinam. Amanoa sinuosa is proposed as a new name for the later homonym A. robusta Leal. A key to the 13 neotropical species is presented.

Description Of The Tadpoles Of Gastrophryne Pictiventris And Nelsonophryne Aterrima (Anura: Microhylidae), With A Review Of Morphological Variation In Free-Swimming Microhylid Larvae, Maureen A. Donnelly, Rafael O. De Sá, Craig Guyer

Biology Faculty Publications

The tadpoles of Gastrophryne pictiventris and Nelsonophryne aterrima are described based on material collected in Costa Rica and Panama, respectively. The tadpole of G. pictiventris resembles those of the other species of Gastrophryne. The tadpole of N. aterrima differs from other New World microhylid tadpoles in location of the vent tube (medial), the spiracular opening (ventral to the gut), and eyes (dorsolateral). The unique configuration of mouthparts in N. aterrima is described. Morphological variation in microhylid tadpoles is reviewed and six larval characters are analyzed cladistically for IO New World microhylid genera.

Methyl Salicylate Secretory Cells In Roots Of Viola Arvensis And V. Rafinesquii (Violaceae), W. John Hayden, John Clough

Biology Faculty Publications

The aromatic roots of Viola arvensis and V. rafinesquii were studied in order to determine the chemical nature and anatomical localization of their volatile compounds. Gas chromatography and mass spectroscopy revealed a single detectable volatile compound, methyl salicylate. Light microscopy and differential staining with Sudan III indicates the source of this compound to be enlarged secretory cells located in the endodermis, an unusual position for such cells in roots of angiosperms. The secretory endodermal cells are sporadic, but are more frequent in primary roots than in secondary roots and the lower portion of the hypocotyl. It is hypothesized that …

Flueggea Willd, W. John Hayden

Biology Faculty Publications

Shrubs to large trees with watery sap. Leaves simple, alternate, pinnately veined, stipulate. Flowers unisexual (and the plants dioecious or rarely monoecious), actinomorphic, in axillary clusters; sepals 4-7, imbricate; petals absent; nectary disk lobed or entire; stamens (3)4-7, alternate with the lobes of floral disk, staminodes absent in pistillate flowers; filaments distinct; ovary superior, on a hypogynous disk, (2)3(4)-carpellate, ovules 2 per cell, hemitropous, pistillode present in staminate flowers; styles distinct and 2-lobed or stigmas sessile. Fruit a capsule or baccate, indehiscent. Seeds 2 per cell. [Hayden, 1987; Sherff, 1939b]

Noteworthy Collections: Virginia, W. John Hayden

Biology Faculty Publications

The above collections represent the second record for the state of Virginia, and may well establish the northernmost station for the species in the eastern United States. According to B.L. Lipscomb (Sida 8:320-327. 1980), this Old World weed is spreading throughout the warmer regions of North America. The only previous record of the species from the state is a Fernald specimen from Owl Creek in Virginia Beach. R.W. Tyndall, who found the species in northeastern North Carolina, was unable in 1978 to locate the plant at Fernald's Virginia Beach locality (Castanea 48:277-280. 1983). The Amelia County plants occur in two …

Flora Of Richmond National Battlefield Park, Virginia, W. John Hayden, Melanie Lynn Haskins, Miles F. Johnson, James M. Gardner

Biology Faculty Publications

An inventory of the vascular flora of nine of eleven units of Richmond National Battlefield Park was compiled from 1985 to 1987. Each site was visited during the growing season in two to four week intervals; plant species were identified and recorded in the field and/or collected for later study. A total of 761 different species were identified in the surveyed units, and 2487 individual records of species per particular park unit were noted. Twenty-three percent of the flora consists of exotic species, largely from Eurasia. Voucher specimens are housed in the herbaria of the University of Richmond and Virginia …

Ontogeny Of The Cotyledonary Region Of Chamaesyce Maculata (Euphorbiaceae), W. John Hayden

Biology Faculty Publications

Development of the cotyledonary region in Chamaesyce maculata is described from germination of the seed through formation of the dense mat of branches which characterize this common weed. The cotyledonary node is trilacunar with split-lateral traces. Epicotyl development is limited to a pair ofleaves ("V-leaves") inserted directly above and decussate to the cotyledons. The two V-leaves are also vascularized by three traces and insertion of these traces relative to the vasculature at the immediately subjacent cotyledonary node is asymmetrical; four of the six V-leaf traces arise on one side of the intercotyledonary plane and two arise on the opposite side. …

Histology Of The Ovaries And Fat Bodies Of Chthonerpeton Indistinctum, Rafael O. De Sá, Nibia Berois

Biology Faculty Publications

Histological analysis revealed three different stages in the development of ovarian follicles of Chthonerpeton indistinctum. These stages are identified by (1) location of the oocyte in the ovary wall, (2) oocyte morphology and staining characteristics, (3) development of the zona pellucida, and (4) organization of the follicular layer. Atretic follicles and corpora lutea also were found. Fat bodies are composed of well-developed adipose tissue.

The Identity Of The Genus Neowawraea (Euphorbiaceae), W. John Hayden

Biology Faculty Publications

On the basis of newly acquired morphological evidence gathered in the course of floristic studies, the monotypic genus Neowawraea Rock is now recognized as a species of Flueggea Willd. and is renamed F. neowawraea W. J. Hayden. Taxonomic documentation presented for F. neowawraea includes an expanded morphological description, a map showing its widely scattered distribution in the Hawaiian Islands to which the species is endemic, and discussions of type specimens, common names, and its extreme rarity. The combination of flowers in pedicellate axillary clusters, the lobed staminate disk, pistillode, extrorse anthers, hemitropous ovules, and, especially, the smoothish dry seeds with …

Anatomy And Affinities Of Penthorum, Melanie Lynn Haskins, W. John Hayden

Biology Faculty Publications

The genus Penthorum L. consists of two species of perennial herbs, P. sedoides of eastern North America and P. chinense of eastern Asia. Penthorum has long been considered intermediate between Crassulaceae and Saxifragaceae. An anatomical study of both species was undertaken to contribute to a better understanding of the relationships of these plants. Prominent anatomical features of Penthorum include: an aerenchymatous cortex and closely-spaced collateral vascular bundles of stems; one-trace unilacunar nodes; brochidodromous venation, rosoid teeth bearing hydathodes, and anomocytic stomata of leaves; angular vessel elements with many-barred scalariform perforation plates and alternate to scattered intervascular pits; thin-walled nonseptate fiber-tracheids; …

Spermatogenesis And Histology Of The Testes Of The Caecilian, Chthonerpeton Indistinctum, Rafael O. De Sá, Nibia Berois

Biology Faculty Publications

Macroscopically, the testes of Chthonerpeton indistinctum consist of a series of oval lobes. The number and size of lobes is variable in Chthonerpeton indistinctum. Histologically, the testes are divided into structural units, locules separated by septa of connective tissue. Inside of each locule spermatogenesis takes place in cysts of germinal cells that divide synchronically. Chthonerpeton indistinctum is divided chronologically into four stages of development based on the shape of the nuclei of the spermatids and their arrangement in the cyst: (1) round, (2) kidney shaped, (3) attenuate, and (4) bouquet arrangement.

Genetic Engineering, W. John Hayden

Biology Faculty Publications

Why do so many biologists express so little concern for biology as a whole and such contempt for fields other than their own? The current debate on release of genetically engineered organisms into the environment, specifically, J. E. Fox's recent reply (Letters, 18 Oct., p. 237) to an earlier letter by R. K. Colwell et al. (12 July, p. 111), offers a case in point.

Eryngium Prostratum In Central Virginia, W. John Hayden

Biology Faculty Publications

While visiting Pocahontas State Park in Chesterfield County, Virginia during the fall of 1984, an unfamiliar blue-flowered plant was observed growing near the upper reaches of Swift Creek Lake. This proved to be Eryngium prostratum Nuttall ex DC., a species common near bodies of water in the southeast U.S. While several standard floras include Virginia in the distribution of this plant (Fernald 1950, Gleason 1952, Gleason & Cronquist 1963, Radford et al 1968, Godfrey & Wooten 1981), the only counties for which it is recorded in Harvill et al (1981) are along the extreme southern border of the state, i.e., …

Gametogenesis En Chthonerpeton Indistinctum (Gymnophiona: Typhlonectidae), Rafael O. De Sá, Nibia Berois

Biology Faculty Publications

RESUMEN:

Histológicamente, las gónadas de Chthonerpeton indistinctum son similares a las ya descritas para otras especies del orden Gymnophiona. Los lóbulos testiculares se hallan compartimentados en unidades estructurales denominadas lóculos. La espermatogénesis ocurre en cistos sincrónicos de células germinales. Durante la espermiogénesis se identifican cuatro estados consecutivos de desarrollo: 1) cistos de espermátidas redondeadas; 2) cistos de espermátidas arrifionadas; 3) cistos de espermátidas bastoniformes y 4) espermátidas en ramillete.

El análisis histológico de los ovarios mostró la presencia de tres estados en la maduración de los folículos ováricos. Estas etapas pueden ser identificadas de acuerdo a: 1) ubicación del folículo …

Estructura Del Ovario De Chthonerpeton Indistinctum (Reinhardt Y Lutken, 1862) (Amphibia: Gymnophiona) Durante La Gestación, Rafael O. De Sá, Nibia Berois

Biology Faculty Publications

La morfología e histología del ovario para varias especies del orden Gymnophiona ha sido reportada por Wake (1968, J. Morphol. 126, 291) y para C. indistinctum por los autores (1984, XXIV Reunión de Comunicaciones, Soc. Zool. Uruguay).

Continuando con los estudios sobre el aparato genital de esta especie vivípara, se presenta ahora la estructura del ovario durante la gestación.

Se procesaron con fines histológicos, ovarios de dos hembras grávidas colectadas en las costas de los Dep. Montevideo y San José en abril, 1983. El número de ejemplares trabajados es muy reducido dado lo difícil que resulta su colecta …

Morfología Y Funciones Del Conducto De Müller De Chthonerpeton Indistinctum (Reinhardt Y Lutken, 1862) (Amphibia: Gymnophiona) En Relación Con Los Mecanismos Reproductivos De La Especie, Nibia Berois, Rafael O. De Sá

Biology Faculty Publications

El orden Gymnophiona comprende anfibios ápodos, ciegos, de hábitos cavícolas y acuáticos. Como consecuencia de estos hábitos resulta muy difícil su colecta, lo cual redunda en un escaso conocimiento de la biología del orden y sobre las relaciones filogenéticas del mismo.

Una de las características más particulares del grupo se refiere a la modalidad reproductora, ya que aproximadamente el 50% de las especies presentan viviparidad y la totalidad de ellas tienen fecundación interna. Es por esta razón que el estudio del aparato reproductor ha sido encarado por diversos autores, en especial por Wake (1968, J. Morphol. 126: 291) con una …

University Of Richmond Herbarium, W. John Hayden

Biology Faculty Publications

The Department of Biology at the University of Richmond, Richmond, Virginia, maintains an herbarium of approximately 15,000 specimens. Although recently assigned the acronym URV, this collection has not yet been included in Index Herbariorum and, consequently, few botanists outside of Virginia are aware of its existence. This note provides a brief account of the history of URV, a summary of its contents, and a short bibliography of works pertaining to the collection.

Wood Anatomy And Relationships Of Neowawraea (Euphorbiaceae), W. John Hayden, Dorthe S. Brandt

Biology Faculty Publications

Wood anatomy of three specimens of Neowawraea phyllanthoides Rock, a rare and endangered member of Euphorbiaceae endemic to the Hawaiian Islands, is described and compared with woods of other genera of subfamily Phyllanthoideae. Neowawraea has often been associated or synonymized with Drypetes Vahl. Wood of Neowawraea is diffuse porous, perforation plates are simple, imperforate tracheary elements are thin-walled septate fiber-tracheids, rays are heterocellular and crystalliferous, and axial xylem parenchyma is restricted to a few scanty paratracheal and terminal cells. In several respects these results differ from earlier published descriptions of the wood of this taxon; these earlier descriptions are shown …

Wood Anatomy And Relationships Of Betula Uber, W. John Hayden, Sheila M. Hayden

Biology Faculty Publications

Wood anatomy of Betula uber (Ashe) Fernald is described and compared with woods of other birches belonging to series Humiles and series Costatae. Anatomically, wood of B. uber is typical of birches in general. On the basis of pore size and frequency, fiber characteristics, axial xylem parenchyma distribution, and absence of aggregate rays, it is argued that B. uber is properly classified in series Costatae. Resolution of its relationships within series Costatae is not apparent from wood data.

Curatorial News From The University Of Richmond Herbarium, W. John Hayden

Biology Faculty Publications

The Department of Biology at the University of Richmond maintains a herbarium of approximately 15,000 specimens. In an effort to make this collection better known to Virginia botanists, this note describes some recent curatorial improvements upon the UR Herbarium and outlines its contents.

Systematics And Palynology Of Picrodendron Further Evidence For Relationship With The Oldfieldioideae (Euphorbiaceae), W. John Hayden, W. T. Gillis, D. E. Stone, C. R. Broome, G. L. Webster

Biology Faculty Publications

Although known to botanical science for 285 years, the genus Picrodendron Planchon has been poorly understood for most of this time. The most pervasive problem has been that of discerning familial relationships, and there have been additional difficulties in typifying the generic name (Hayden & Reveal, 1980) and in distinguishing its three nominate species. This paper provides a systematic treatment for Picrodendron and demonstrates its relationships with Euphorbiaceae subfam. Oldfieldioideae Kohler & Webster as evidenced by data on gross morphology, palynology, anatomy, and cytology.

Leptodactylus Podicipinus (Cope, 1862) Y Leptodactylus Chaquensis Cei, 1959 (Anura, Leptodactylidae), En El Uruguay, Raúl Vaz-Ferreira, Rafael O. De Sá, Federico Achaval, Annelise Gebrau

Biology Faculty Publications

En base al estudio de las colecciones obtenidas principalmente en excursiones a la zona NO de Uruguay con motivo del relevamiento faunístico del área de Salto Grande, se avanzó considerablemente en el conocimiento de la distribución de Leptodactylus podicipinus (Cope, 1862) y Leptodactylus chaquensis (Cei, 1950), en nuestro país.

Se cita el material estudiado y las observaciones de campo realizadas; se dan los caracteres generales de ambas especies, se describen los hábitats, y se tratan las características de las vocalizaciones.

Jamaican Blue-Green Algae Collections Of J.C. Strickland, W. John Hayden

Biology Faculty Publications

Professor John C. Strickland (1915-1980) devoted much of his life to teaching biology, botany, and phycology at the University of Richmond. Throughout his academic career he maintained a keen interest in the Myxophyceae, or blue-green algae, studying their culture, cytology, and taxonomy (Drouet & Strickland, 1942; Strickland, 1940, 1946). Most of his collections of these and other algae were made in Virginia and are housed in the herbarium maintained by the Department of Biology, University of Richmond. However, he also made four trips to Jamaica in the years 1966-1971 before his health deteriorated to the extent that field work, and …

Observaciones Sobre La Biología De Phyllomedusa Iheringi Boulenger, 1885, (Anura, Hylidae), Rafael O. De Sá, Annelise Gerhau

Biology Faculty Publications

RESUMEN:

Se presentan las características que permiten diferenciar las larvas de Phyllomedusa iheringi de las de otros anuros que habitan en nuestro territorio, junto con algunas observaciones sobre la biología de esta especie poco conocida en el Uruguay.

SUMMARY:

The characteristics that differentiate the larvae of Phyllomedusa iheringi from other anurans that live in Uruguay are presented here. Some observations on the biology of this species are also recorded.

Proposal For The Conservation Of The Generic Name Picrodendron Grisebach (1859) Against Picrodendron Planchon (1846) (Euphorbiaceae), W. John Hayden, James L. Reveal

Biology Faculty Publications

Petalacte has been in continuous use for this genus of 1-3 species since proposed by D. Don. The only exception was Lessing's misuse of Petalolepis Cassini, from which he excluded the two original Australian species and substituted two from S. Africa. Lessing was not followed by later authors.T he genus was recently revised by Lundgren (in Bot. Notiser 127: 119-124. 1974) and this revision may be consulted for full references.

Comparative Anatomy And Systematics Of Picrodendron, Genus Incertae Sedis, W. John Hayden

Biology Faculty Publications

This study of the vegetative anatomy of Picrodendron and some of its putative relatives has been undertaken in order better to understand its natural relationships. Despite the number of anatomical studies in the literature (Jadin, 1901; Solereder, 1908; Boas, 1913; Webber, 1936; Heimsch, 1942; Record & Hess, 1943; Metcalfe & Chalk, 1950), our information on the anatomy of Picrodendron is still incomplete· for example, nodal and petiolar anatomy has apparently never been described. Furthermore, with the exception of Record and Hess (1943), who discussed Picrodendron in a family by itself, other anatomists have compared Picrodendron only with members of Simaroubaceae, …