Life Sciences Commons^™

Leaf Optical And Indirect Lai Measurements In Wheat And Alfalfa At Maciv: Agmet Progress Report 89-4, E. A. Walter-Shea, B. L. Blad

School of Natural Resources: Documents and Reviews

No abstract provided.

Adding Value To Crop Production Systems By Integrating Forage Cover Crop Grazing, Robert B. Mitchell, Daren D. Redfearn, Kenneth P. Vogel, Terry J. Klopfenstein, Galen Erickson, P. Steven Baenziger, Bruce E. Anderson, Mary E. Drewnoski, Jay Parsons, Steven D. Masterson, Marty R. Schmer, Virginia L. Jin

Department of Agronomy and Horticulture: Faculty Publications

In addition to their value as cereal grains, wheat (Triticum aestivum L.) and triticale (× Triticosecale Wittmack) are important cool-season annual forages and cover crops. Yearling steer (Bos taurus) performance was compared in the spring following autumn establishment as for age cover crops after soybean [Glycine max (L.) Merr.] grain harvest. Replicated pastures (0.4 ha) were no-till seeded in three consecutive years into soybean stubble in autumn, fertilized, and grazed the following spring near Ithaca, Nebraska, USA. Each pasture (n = 3) was continuously stocked in spring with four yearling steers (380 ± 38 kg) for …

Winter Hardy Small Cereals For Grazing Or Silage In Eastern Nebraska, Abigail Sartin

Department of Animal Science: Dissertations, Theses, and Student Research

Two, 3-year studies were conducted to evaluate the value of cereal rye, winter wheat, and winter triticale as forage sources for either for silage or early spring grazing. For silage, the objective was to evaluate species and maturity effects on dry matter yield, nutritive value, and fermentation of small cereal silage. There are no major differences between wheat, rye, or triticale in terms of the relative timing of each species reached each maturity stage. There are no differences among species in dry matter (DM) yield, except for wheat which tends to decline at soft dough due to senescence of the …

Gene Expression Changes Linked To Phenylpropanoid-Based Resistance To Fusarium Head Blight Of Wheat, Shiv Singla

Department of Plant Pathology: Dissertations, Theses, and Student Research

Fusarium graminearum is a devastating pathogen of wheat that causes Fusarium head blight (FHB) and contaminates the grain with the mycotoxin deoxynivalenol (DON). Resistance to FHB is quantitative and it is important to identify additional genes conferring resistance against it. The goal of this thesis was to examine if the constitutive expression of two sorghum phenylpropanoid pathway genes, SbCCoAOMT (encoding caffeoyl-CoA O-methyltransferase) and SbC3’H (encoding p-coumarate 3-hydroxylase), in the moderately-susceptible spring wheat CB037 can provide Type-I and Type-II resistance to F. graminearum and determine the underlying mechanisms of the enhanced resistance. The constitutive expression lines (CCoAOMT413, CCoAOMT421, C3H112, …

Predicting Site‑Specific Economic Optimal Nitrogen Rate Using Machine Learning Methods And On‑Farm Precision Experimentation, Alfonso De Lara, Taro Mieno, Joe D. Luck, Laila A. Puntel

Department of Agronomy and Horticulture: Faculty Publications

Applying at the economic optimal nitrogen rate (EONR) has the potential to increase nitrogen (N) fertilization efficiency and profits while reducing negative environmental impacts. On-farm precision experimentation (OFPE) provides the opportunity to collect large amounts of data to estimate the EONR. Machine learning (ML) methods such as generalized additive models (GAM) and random forest (RF) are promising methods for estimating yields and EONR. Twenty OFPE N trials in wheat and barley were conducted and analyzed with soil, terrain and remote-sensed variables to address the following objectives: (1) to quantify the spatial variability of winter crops yield and the yield response …

6k1, Nia-Vpg, Nia-Pro, And Cp Ofwheat Streak Mosaic Virus Are Collective Determinants Of Wheat Streak Mosaic Disease In Wheat, Satyanarayana Tatineni, Jeffrey Alexander, Haritha Nunna

Department of Plant Pathology: Faculty Publications

Wheat streak mosaic virus (WSMV; genus Tritimovirus, family Potyviridae) is the causal agent of the most economically important wheat streak mosaic disease of wheat (Triticum aestivum) in the Great Plains region of theUnited States.WSMVdeterminants responsible forwheat streak mosaic disease in wheat are unknown. Triticum mosaic virus (TriMV), a wheatinfecting virus,was used as an expression vector for the transient expression of each of the WSMV-encoded cistrons in wheat. WSMV-encoded 6K1, NIa-VPg, NIa-Pro, and CP cistrons in TriMV elicited symptoms specific to different stages of wheat streak mosaic disease without significantly affecting the genomic RNA accumulation. WSMV 6K1 …

6k1, Nia-Vpg, Nia-Pro, And Cp Ofwheat Streak Mosaic Virus Are Collective Determinants Of Wheat Streak Mosaic Disease In Wheat, Satyanarayana Tatineni, Jeffrey Alexander, Haritha Nunna

Department of Plant Pathology: Faculty Publications

Wheat streak mosaic virus (WSMV; genus Tritimovirus, family Potyviridae) is the causal agent of the most economically important wheat streak mosaic disease of wheat (Triticum aestivum) in the Great Plains region of theUnited States.WSMVdeterminants responsible forwheat streak mosaic disease in wheat are unknown. Triticum mosaic virus (TriMV), a wheatinfecting virus,was used as an expression vector for the transient expression of each of the WSMV-encoded cistrons in wheat. WSMV-encoded 6K1, NIa-VPg, NIa-Pro, and CP cistrons in TriMV elicited symptoms specific to different stages of wheat streak mosaic disease without significantly affecting the genomic RNA accumulation. WSMV 6K1 …

Evaluation Of Wheat-Specific Peptide Targets For Use In The Development Of Elisa And Mass Spectrometry-Based Detection Methods, Jessica Humphrey

Department of Food Science and Technology: Dissertations, Theses, and Student Research

Individuals with IgE-mediated wheat allergy and celiac disease must avoid the consumption of wheat. Those with celiac disease must also avoid rye and barley in addition to wheat as these make up the gluten-containing grains. To ensure products are accurately labeled, ELISA methods may be used to monitor compliance with food allergen and gluten-free regulations. Currently, available ELISA methods for wheat detection utilize monoclonal antibodies that target peptides from the gluten fraction of proteins, and therefore, also detect rye and barley. Limitations such as differing antibody reactivity and unequal gluten content among wheat, rye, and barley have led to inconsistent …

Impacts Of Added Roughage On Growth Performance, Digestibility, Ruminal Fermentation, And Ruminal Ph Of Feedlot Steers Fed Wheat-Based Feedlot Diets Containing 30% Modified Distillers Grains With Solubles, Wayde J. Pickinpaugh, Bryan W. Neville, Rebecca L. Moore, Joel S. Caton

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Two experiments were conducted to evaluate the inclusion rate roughage in wheat-based diets containing modified distillers grains with solubles (MDGS) on feedlot performance (Feedlot Experiment), as well as digestibility, ruminal pH, and ruminal fermentation characteristics (Digestibility Experiment). The feedlot experiment utilized 72 Angus steers (392 ± 46.3 kg initial body weight) which were randomly assigned to 1 of 12 pens, 3 pens per treatment, to evaluate feedlot performance and carcass characteristics. Dietary treatments were 1) control; 10% roughage, 2) 12% roughage, 3) 14% roughage, and 4) 16% roughage. The digestibility experiment used four ruminally and duodenally cannulated steers (393 ± …

Editorial: Genomics-Enabled Triticeae Improvement, Xue-Feng Ma, Xianchun Xia, Shuyu Liu, P. Stephen Baenziger, Hakan Özkan

Department of Agronomy and Horticulture: Faculty Publications

No abstract provided.

Source Of Off-Type Frequencies In Winter Wheat Varieties, Michaela Benes

Honors Theses

The enzyme polyphenol oxidase (PPO) is undesirable in most wheat products due to the discoloration it causes. Purifying wheat varieties through selective breeding is the best way at getting rid of PPO in a variety. The variety NW15443 was planted in both a field in McCook, NE, and in a greenhouse in Lincoln, NE. Individual heads were snapped from the field and the greenhouse seed was harvested in bulk. After completing a PPO test of the seed, it was determined that the percentages of off-type frequencies from the field seed and the greenhouse seed were 11% and 3.6%, respectively. Using …

Coleoptile Length Comparison Of Three Winter Small Grain Cereals Adapted To The Great Plains, M. Alam, M. Kashif, Amanda Easterly, F. Wang, J. D. Boehm Jr., P. Stephen Baenziger

Department of Agronomy and Horticulture: Faculty Publications

Successful crop stand establishment is critical to realize high yield potential, which is dependent on depth of seed placement to access soil moisture. The coleoptile determines sowing depth by its length and ability to emerge from depth. This study was conducted to assess coleoptile length among three sets of three Great Plains winter small grain cereals—wheat (Triticum aestivum L.), barley (Hordeum vulgare L.) and triticale (X triticosecale Wittm.)—and to evaluate the effect of the Rht-B1b dwarfing allele on coleoptile length in wheat and triticale. Fifty seeds of each genotype were sown in wet germination paper in two …

Evaluation Of Grain Type And Processing Method On Steer Performance, Carcass Characteristics, And Nutrient Digestion, Caitlin Coulson

Department of Animal Science: Dissertations, Theses, and Student Research

A feedlot finishing study evaluated the effect of replacing corn with dry-rolled wheat in diets containing wet distillers grains plus solubles (WDGS; Exp 1). Two studies were conducted to evaluate the effect of corn type [dry corn (DC), high-moisture corn (HMC), or a blend of dry and high-moisture corn (BLEND)] processed with either AutomaticRoller Mill (ROLL) or hammer mill (HAMMER) on steer performance, carcass traits, and nutrient digestibility (Exp. 2 and 3). In Exp. 1, steers were fed diets containing 100% dry-rolled corn (DRC) or a 50:50 blend of dry-rolled corn and wheat (WHEAT) in diets containing 12 …

Editorial: Gluten, From Plant To Plate: Implications For People With Celiac Disease, Michelle Lisa Colgrave, Katharina Anne Scherf, Melanie Downs, Alberto Caminero

Department of Food Science and Technology: Faculty Publications

No abstract provided.

High Plains Wheat Mosaic Virus: An Enigmatic Disease Of Wheat And Corn Causing The High Plains Disease, Satyanarayana Tatineni, Gary Hein

Department of Entomology: Faculty Publications

Brief history: In 1993, severe mosaic and necrosis symptoms were observed on corn (maize) and wheat from several Great Plains states of the USA. Based on the geographical location of infections, the disease was named High Plains disease and the causal agent was tentatively named High Plains virus. Subsequently, researchers renamed this virus as maize red stripe virus and wheat mosaic virus to represent the host and symptom phenotype of the virus. After sequencing the genome of the pathogen, the causal agent of High Plains disease was officially named as High Plains wheat mosaic virus. Hence, High Plains virus, …

Intervention Of Climate-Smart Practices In Wheat Under Rice-Wheat Cropping System In Nepal, Janma Jaya Gairhe, Mandeep Adhikari, Deepak Ghimire, Arun Khatri-Chhetri, Dinesh Panday

Department of Agronomy and Horticulture: Faculty Publications

Besides a proper agronomic management followed by Nepalese farmers, wheat (Triticum aestivum L.) production has been severely affected by changing climate. There are many interventions, including climate-smart practices, to cope with this situation and possibly enhance crop and soil productivity. Field experiments were set up in a randomized complete block design with six treatments (TRT) with four replications in three locations (LOC) during wheat-growing seasons in Nepal from 2014 to 2016. Treatments included (i) Controlled Practice (CP), (ii) Improved Low (IL), (iii) Improved High (IH), (iv) Climate Smart Agriculture Low (CSAL), (v) Climate Smart Agriculture Medium (CSAM), and (vi) …

Agricultural And Food Security Impacts From The 2010 Russia Flash Drought, Eric D. Hunt, Francesco Femia, Caitlin Werrell, Jordan Christian, Jason A. Otkin, Jeff Basara, Martha Anderson, Tyler R. White, Christopher Hain, Robb Randall, Katie Mcgaughey

Department of Political Science: Faculty Publications

The flash drought and its associated heat wave that affected western Russia in the summer of 2010 had significant cascading agricultural and socioeconomic impacts. Drought indicators sensitive to soil moisture and evapotranspiration (ET) showed that the flash drought began in June 2010, then intensified rapidly and expanded to cover much of western Russia. By early July, almost all of the major wheat producing regions of Russia were experiencing extreme water stress to the winter and spring wheat crops. The timing of the onset of the flash drought was particularly devastating as the period of most rapid intensification overlapped with the …

Developing A Method To Contain The Feeding Of The Wheat Curl Mite (Aceria Tosichella Keifer), Pierce Leef, Gary Hein

UCARE Research Products

To better understand the ecology and epidemiology of the wheat curl mite (Aceria tosichella Keifer) as a vector of wheat streak mosaic virus (WSMV), it is necessary to develop a method to confine the microscopic mites and their infection to specific areas of their host plants, particularly wheat and corn. In this study, corn was the plant of interest. The device developed for this mite-confining method was made by removing the lens of a phone camera lens clip and gluing a 2.5 cm x 4.5 cm piece of wood to the other arm of the clip. Clips were utilized by …

Phenology And Dispersal Of The Wheat Stem Sawfly (Hymenoptera: Cephidae) Into Winter Wheat Fields In Nebraska, Chris T. Mccullough, Gary L. Hein, Jeff Bradshaw Dr.

Department of Entomology: Faculty Publications

Historically, the wheat stem sawfly, Cephus cinctus Norton was a pest in spring wheat-growing regions of the northern Great Plains. However, in the 1980s, it was found infesting winter wheat fields in Montana. Infestations were first detected in western Nebraska in the 1990s, and have since spread throughout the Nebraska Panhandle. Larval damage occurs from stem-mining, but stem girdling that results in lodged stems that are not harvested results in the greatest yield losses.The biology and phenology of the wheat stem sawfly are well described in the northern portion of its range, but they are lacking in Colorado, southeast Wyoming, …

The Lateral Root Density Gene Regulates Root Growth During Water Stress In Wheat, Dante F. Placido, Jaspreet Sandhu, Shirley Sato, Natalya Nersesian, Truyen Quach, Thomas Clemente, Paul Staswick, Harkamal Walia

Department of Agronomy and Horticulture: Faculty Publications

Drought stress is the major limiting factor in agriculture. Wheat, which is the most widely grown crop in the world, is predominantly cultivated in drought-prone rainfed environments. Since roots play a critical role in water uptake, root response to water limitations is an important component for enhancing wheat adaptation. In an effort to discover novel genetic sources for improving wheat adaptation, we characterized a wheat translocation line with a chromosomal segment from Agropyron elongatum, a wild relative of wheat, which unlike common wheat maintains root growth under limited-water conditions. By exploring the root transcriptome data, we found that reduced …

The Lateral Root Density Gene Regulates Root Growth During Water Stress In Wheat, Dante F. Placido, Jaspreet Sandhu, Shirley Sato, Natalya Nersesian, Truyen Quach, Thomas E. Clemente, Paul E. Staswick, Harkamal Walia

Department of Agronomy and Horticulture: Faculty Publications

Drought stress is the major limiting factor in agriculture. Wheat, which is the most widely grown crop in the world, is predominantly cultivated in drought-prone rainfed environments. Since roots play a critical role in water uptake, root response to water limitations is an important component for enhancing wheat adaptation. In an effort to discover novel genetic sources for improving wheat adaptation, we characterized a wheat translocation line with a chromosomal segment from Agropyron elongatum, a wild relative of wheat, which unlike common wheat maintains root growth under limited-water conditions. By exploring the root transcriptome data, we found that reduced …

Wheat Growth Monitoring And Yield Estimation Based On Multi-Rotor Unmanned Aerial Vehicle, Zhaopeng Fu, Jie Jang, Yang Gao, Brian Krienke, Meng Wang, Kaitai Zhong, Qiang Cao, Yongchao Tian, Yan Zhu, Weixing Cao, Xiaojun Liu

Department of Agronomy and Horticulture: Faculty Publications

Leaf area index (LAI) and leaf dry matter (LDM) are important indices of crop growth. Real-time, nondestructive monitoring of crop growth is instructive for the diagnosis of crop growth and prediction of grain yield. Unmanned aerial vehicle (UAV)-based remote sensing is widely used in precision agriculture due to its unique advantages in flexibility and resolution. This study was carried out on wheat trials treated with different nitrogen levels and seeding densities in three regions of Jiangsu Province in 2018–2019. Canopy spectral images were collected by the UAV equipped with a multi-spectral camera during key wheat growth stages. To verify the …

Identification, Aggressiveness And Mycotoxin Production Of Fusarium Graminearum And F. Boothii Isolates Causing Fusarium Head Blight Of Wheat In Nebraska, Esteban Valverde-Bogantes

Department of Food Science and Technology: Dissertations, Theses, and Student Research

Fusarium head blight (FHB) is an economically important disease caused by several Fusarium species affecting wheat. Besides causing significant yield losses, infected grain may be contaminated with mycotoxins, primarily trichothecenes, that are harmful to both humans and animals. In recent years, reports of shifts in FHB pathogen populations in North America have highlighted the need for increased vigilance. This dissertation describes a four-year survey evaluating the species, trichothecene genotype and phenotypic diversity of FHB pathogens infecting Nebraska wheat. Most of the isolates were identified as F. graminearum (n=67). Additional species included F. boothii (n=3), F. poae (n=2) and F. acuminatum …

Effects Of Different Water And Nitrogen Regimens On Yield Of Winter Wheat Produced In Nebraska, Joseph Emory Davis

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Wheat is the 3rd most prominent crop in the USA and approximately 50% is exported annually. Nebraska wheat production is 11^th in the country, and it plays a major role in the state's agricultural economy, especially in western NE. Generally, wheat is grown under dryland conditions and the region grows much more wheat on unirrigated land than it does on irrigated. However, deficit irrigation has shown great value in producing high yielding wheat with much less water than needed for other crops. Finding new ways to leverage irrigation in wheat production may help address the need to produce food …

Addressing The Challenges Facing Wheat Production: Nebraska And International Breeding Efforts, Sarah Blecha

Doctor of Plant Health Program: Dissertations and Student Research

Bread wheat, Triticum aestivum L., provides 20 percent of the global daily calorie intake. It is the third most important food crop, after rice and corn. Biotic challenges significantly reduce wheat yield; chemical control can be a solution but can be cost prohibitive for subsistence farmers. For many farmers, genetic resistance to biotic stresses can be the most cost effective solution.

The International Center for Agricultural Research in the Dry Areas (ICARDA) and the Nebraska Small Grains Breeding Program have been addressing these wheat production challenges. ICARDA is part of an international research consortium to increase wheat yield and tolerance …

Estimating Percentages Of Fusarium-Damaged Kernels In Hard Wheat By Near-Infrared Hyperspectral Imaging, Stephen R. Delwiche, I. Torres Rodriguez, S. R. Rausch, R. A. Graybosch

Department of Agronomy and Horticulture: Faculty Publications

Fusarium head blight (FHB) is among the most common fungal diseases affecting wheat, resulting in decreased yield, low-density kernels, and production of the mycotoxin deoxynivalenol, a compound toxic to humans and livestock. Human visual analysis of representative wheat samples has been the traditional method for FHB assessment in both official inspection and plant breeding operations. While not requiring specialized equipment, visual analysis is dependent on a trained and consistent workforce, such that in the absence of these aspects, biases may arise among inspectors and evaluation dates. This research was intended to avoid such pitfalls by using longer wavelength radiation than …

Water Productivity Of Rainfed Maize And Wheat: A Local To Global Perspective, Juan I. Rattalino Edreira, Nicolas Guilpart, Victor Sadras, Kenneth G. Cassman, Martin K. Van Ittersum, Rene L.M. Schils, Patricio Grassini

Department of Agronomy and Horticulture: Faculty Publications

Water productivity (WP) is a robust benchmark for crop production in relation to available water supply across spatial scales. Quantifying water-limited potential (WPw) and actual on-farm (WPa) WP to estimate WP gaps is an essential first step to identify the most sensitive factors influencing production capacity with limited water supply. This study combines local weather, soil, and agronomic data, and crop modeling in a spatial framework to determine WPw and WPa at local and regional levels for rainfed cropping systems in 17 (maize) and 18 (wheat) major grain-producing countries representing a wide range of cropping systems, from intensive, highyield maize …

Wheat Streak Mosaic Virus Coat Protein Is A Host-Specific Long-Distance Transport Determinant In Oat, Satyanarayana Tatineni

Department of Plant Pathology: Faculty Publications

Viral determinants involved in systemic infection of hosts by monocot-infecting plant viruses are poorly understood. Wheat streak mosaic virus (WSMV, genus Tritimovirus, family Potyviridae) exclusively infects monocotyledonous crops such as wheat, oat, barley, maize, triticale, and rye. Previously, we reported that WSMV CP amino acids 36–84 are expendable for systemic infection of wheat, maize, barley and rye. In this study, the role of coat protein (CP) in systemic infection of oat by WSMV was examined by using a series of viable deletion mutants. WSMV bearing deletions within or encompassing all of amino acids 36–57 efficiently infected oat, indicating that …

Review Of Nutraceuticals And Functional Properties Of Whole Wheat, Rachana Poudel, Madhav Bhatta

Department of Food Science and Technology: Faculty Publications

Wheat (Triticum aestivum L.) is one of the most commonly cultivated and consumed cereals throughout the world. Though phytochemicals and antioxidants in the cereal grains have not been studied as in fruits and vegetables, given the role of wheat in our diet plate, it is a given of primary importance to understand the chemistry of our major food, wheat. The presence of diverse polyphenols and their action against leading cause of death, including heart diseases, cancer, obesity, and diabetes, widens the scope of wheat. Phytochemicals such as phenolic acids, alkylresorcinols, flavonoids, phytosterols, and carotenoids are present in whole wheat. …

Expression Of The Maize Dof1 Transcription Factor In Wheat And Sorghum, Pamela A. Pena, Truyen Quach, Shirley Sato, Zhengxiang Ge, Natalya Nersesian, Taity Changa, Ismail M. Dweikat, Madhavan Soundararajan, Tom E. Clemente

Department of Agronomy and Horticulture: Faculty Publications

Nitrogen is essential for plant growth and development. Improving the ability of plants to acquire and assimilate nitrogen more efficiently is a key agronomic parameter that will augment sustainability in agriculture. A transcription factor approach was pursued to address improvement of nitrogen use efficiency in two major commodity crops. To this end, the Zea mays Dof1 (ZmDof1) transcription factor was expressed in both wheat (Triticum aestivum) and sorghum (Sorghum bicolor) either constitutively, UBI4 promoter from sugarcane, or in a tissue specific fashion via the maize rbcS1 promoter. The primary transcription activation target of ZmDof1 …