Life Sciences Commons^™

Association Of Excessive Precipitation And Agricultural Land Use With Honey Bee Colony Performance, Gabriela M. Quinlan, Rufus Isaacs, Clint R. V. Otto, Autumn H. Smart, Meghan O. Milbrath

Department of Entomology: Faculty Publications

Context From landscape variables to weather, multiple environmental factors affect honey bees and other pollinators. Detailed honey bee colony assessments in a variety of landscape and weather conditions offer the opportunity to develop a mechanistic understanding of how landscape composition, configuration, and weather are associated with colony nutrition, demography, and productivity.

Objectives Our objective was to test if weather and landscape characteristics (e.g., agricultural versus forested land use) are associated with different honey bee colony outcomes (foraged nectar mass, foraged pollen mass, pupal population size, and adult population size change).

Methods We collected detailed colony measurements on over 450 honey …

Diet Analysis Reveals Pre-Historic Meals Among The Loma San Gabriel At La Cueva De Los Muertos Chiquitos, Rio Zape, Mexico (600–800 Ce), Elisa Pucu, Julia Russ, Karl Reinhard

Karl Reinhard Publications

Coprolites have been a source of study for archeologists due to several reasons: they not only provide information on the life and nutritional habits of ancient individuals but also on their health. In this paper, we processed 10 coprolites collected at La Cueva de Los Muertos Chiquitos (600–800 CE), Rio Zape, Mexico, with acetolysis solution for pollen analysis. The number of pollen grains/gram of each coprolite sample was quantified along with the macroscopic remains of these samples. The main food item ingested by the population was maize, followed by Agave. Squash blossoms were also part of their food source …

Confusing A Pollen Grain With A Parasite Egg: An Appraisal Of “Paleoparasitological Evidence Of Pinworm (Enterobius Vermicularis) Infection In A Female Adolescent Residing In Ancient Tehran”, Morgana Camacho, Karl Reinhard

Karl Reinhard Publications

There is often the risk of confusing pollen grains with helminth eggs from archaeological sites. Thousands to millions of pollen grains can be recovered from archaeological burial sediments that represent past ritual, medication and environment. Some pollen grain types can be similar to parasite eggs. Such a confusion is represented by the diagnosis of enterobiasis in ancient Iran. The authors of this study confused a joint-pine (Ephedra spp.) pollen grain with a pinworm egg. This paper describes the specific Ephedra pollen morphology that can be confused with pinworm eggs.

Protocol For Enrichment Of The Membrane Proteome Of Mature Tomato Pollen, Puneet Paul, Palak Chaturvedi, Anida Mesihovic, Arindam Ghatak, Wolfram Weckwerth, Enrico Schleiff

Department of Agronomy and Horticulture: Faculty Publications

We established and elaborated on a method to enrich the membrane proteome of mature pollen from economically relevant crop using the example of Solanum lycopersicum (tomato). To isolate the pollen protein fraction enriched in membrane proteins, a high salt concentration (750 mM of sodium chloride) was used. The membrane protein-enriched fraction was then subjected to shotgun proteomics for identification of proteins, followed by in silico analysis to annotate and classify the detected proteins.

Palynological Investigation Of Mummified Human Remains, Karl Reinhard, Marina Milanello Do Amaral, Nicole Wall

Karl Reinhard Publications

Pollen analysis was applied to a mummified homicide victim in Nebraska, U.S.A., to determine the location of death. A control sample showed the normal ambient pollen in the garage crime scene. Ambient windborne types, common in the air of the region, dominated the control. Internal samples were analyzed from the sacrum, intestine, and diaphragm. Microfossils were recovered from the rehydrated intestine lumen. The intestinal sample was dominated by Brassica (broccoli). The sacrum sample was high in dietary types but with a showing of ambient types. The pollen from the diaphragm was dominated by ambient pollen similar to the control samples. …

Muts Homolog1 Silencing Mediates Orf220 Substoichiometric Shifting And Causes Male Sterility In Brassica Juncea, Na Zhao, Xinyue Xu, Yashitola Wamboldt, Sally Ann Mackenzie, Xiaodong Yang, Zhongyuan Hu, Jinghua Yang, Mingfang Zhang

Department of Agronomy and Horticulture: Faculty Publications

Cytoplasmic male sterility (CMS) has consistently been associated with the expression of mitochondrial open reading frames (ORFs) that arise from genomic rearrangements. Spontaneous fertility reversion in CMS has been observed in several cases, but a clear understanding of fertility reversion controlled by nuclear genetic influences has been lacking. Here, we identified spontaneous fertile revertant lines for Brassica juncea CMS cytoplasm in which the mitochondrial genome has undergone substoichiometric shifting (SSS) to suppress ORF220 copy number. We placed ORF220, with or without a mitochondrial targeting presequence, under the control of the CaMV35S and AP3 promoters in Arabidopsis to confirm that ORF220 …

Establishing Tobacco Origin From Pollen Identification: An Approach To Resolving The Debate, Shane Williams, Shelby Hubbard, Karl Reinhard, Sérgio Augusto De Miranda Chaves

Karl Reinhard Publications

Previous research into pollen content of tobacco resulted in a debate. We address this debate and determine that pollen analysis may be able to assist with identifying geographical origin of tobacco. However, the value of any results should be assessed on a case-by-case regional basis until sufficient database information is available for an objective interpretation to be undertaken on a global basis. As a first step toward developing comparative data for South America, we analyzed a tobacco sample from Brazil in an effort to identify signature taxa from the state of Minas Gerais. We also assessed the role of honey …

Pollen Interception By Linyphiid Spiders In A Corn Agroecosystem: Implications For Dietary Diversification And Risk-Assessment, Julie A. Peterson, Susan Romero, James D. Harwood

West Central Research and Extension Center, North Platte

Dietary diversification, including consumption of plant tissues such as pollen, can enhance the fecundity of generalist predators, resulting in improved control of pest prey. Supplemental pollen feeding has been observed in many natural enemies, including sheet-web spiders (Araneae: Linyphiidae), which represent a major component of food webs in agroecosystems. Their horizontal, ground-based webs have the potential to intercept pollen grains during anthesis of crop plants, providing the opportunity for consumption of pollen to occur. In laboratory feeding trials, Frontinella communis and Tennesseellum formicum (Araneae: Linyphiidae) readily fed on pollen grains dusted on their webs, with 82 and 92% of spiders …

Penstemon In Your Garden, R. D. Uhlinger, G. Viehmeyer

Nebraska Agricultural Experiment Station: Historical Circulars

A new race of hardy perennials is beginning to appear in American gardens. These are the penstemons, a genus of plants closely related to the familiar snapdragon, which may become important ornamentals as they be come better known to gardeners. The purpose of this bulletin is to show the possibility of the genus as an ornamental and to suggest species and species hybrids of possible value to Great Plains gardeners; to provide information about propagation and culture; and to encourage gardeners to engage in penstemon breeding as an avocation.

Penstemon In Your Garden, G. Viehmeyer

Nebraska Agricultural Experiment Station: Historical Circulars

A new race of hardy perennials is beginning to appear in American gardens. These are the penstemons, a genus of plants closely related to the familiar snapdragon, which may become important ornamentals as they be come better known to gardeners. The purpose of this bulletin is to show the possibility of the genus as an ornamental and to suggest species and species hybrids of possible value to Great Plains gardeners; to provide information about propagation and culture; and to encourage gardeners to engage in penstemon breeding as an avocation.

The Significance Of Xenia Effects On The Kernel Weight Of Corn, T. A. Kiesselbach

Nebraska Agricultural Experiment Station: Historical Research Bulletins

The purpose of this paper is to appraise the xenia effects of the pollen parent on the kernel weight of corn (Zea mays L.), and to interpret so far as possible their genetic, physiologic and applied significance. The conclusions will be based on local experiments and a review of the literature.

The Effects Of Thermal-Neutron Irradiation Of Maize And Barley Kernels, John W. Schmidt, E. F. Frolik

Nebraska Agricultural Experiment Station: Historical Research Bulletins

It is the purpose of this paper to report the effects of thermal-neutron irradiation of maize and barley kernels on subsequent germination, plant establishment, and early plant development. Comparisons are made with equivalent neutron treatments of maize pollen, results of which have been reported in part in previous publications.

The Immediate Effect Of Gametic Relationship And Of Parental Type Upon The Kernel Weight Of Corn, T. A. Kiesselbach

Nebraska Agricultural Experiment Station: Historical Research Bulletins

This investigation to determine the relation of the kernel weight of corn to the source of pollen with which it was fertilized has been made to answer several questions of both practical and technical interest: (1) To what extent is kernel weight affected in the current crop by the "breadth of breeding" or the gametic relationship? (2) What is the relation of the diversity of parental type to the immediate effect of foreign pollen upon the kernel weight of corn? (3) Is the immediate effect of cross-fertilization upon the kernel weight of sufficient importance to justify the annual mixing of …