Life Sciences Commons^™

The Effects Of Xylanase Or An Algal Sourced Zinc Polysaccharide Complex On The Performance Of Laying Hens Fed A Corn And Soybean Diet, Ashley Bigge

Department of Animal Science: Dissertations, Theses, and Student Research

Two studies were conducted to determine the effects of two different feed additives on the performance of laying hens. Study 1 examined the effects of non-starch polysaccharide degrading enzyme inclusion in a low energy diet. Study 2 examined the effects of supplementing a paramylon zinc polysaccharide complex at increasing concentrations. Both studies utilized a randomized complete block design for treatment assignment, and data were analyzed using the Glimmix procedure in SAS 9.4 for windows.

The first study took place July 2014 to March 2015 and consisted of two phases. Each phase tested a positive control, negative control with lower ME …

Analysis Of Morphology And Secretion Mutants In Aspergillus Nidulans, Shelby N. Holaday, Steven D. Harris

UCARE Research Products

Filamentous fungi are important economically and medically due to their capacity to produce secondary metabolites or as human and animal pathogens. The genes and molecular mechanism responsible for secretion is poorly understood. Using classical genetics, we derived temperature sensitive (Ts) fungal mutants in Aspergillis nidulans. These mutants were then analyzed for their secondary metabolite secretion capacity.

In this study, we used the Ts strains of A. nidulans to determine how knockouts affect morphological and protein secretion by examining the phenotypes under the microscope and also by staining the mutants with Congo red staining. Mutants were stained with Congo red to …

Mutant Study Of Sinorhizobium Meliloti Proline Utilization A (Puta), Jacob E. Wilkinson, John J. Tanner, Donald F. Becker

UCARE Research Products

The purpose of this project is to purify and characterize the reaction kinetics of mutant versions the enzyme Proline Utilization A (PutA) in Sinorhizobium meliloti. The enzyme catalyzes the first step in proline metabolism. It has two active sites. The first is proline dehydrogenase (PRODH) which converts proline to pyrroline-5-carboxylate (P5C). The second is P5C dehydrogenase (P5CDH) which converts P5C to glutamate. Although many bacterial organisms have PutA, there are still significant interspecies variations, resulting in an entire family of PutA enzymes. The main difference is the length of the amino acid sequence. This affects the protein’s structure or …

Purification And Characterization Of Cytoplasmic Nadp+- Isocitrate Dehydrogenase, And Amplification Of The Nadp+-Idh Gene From The Wing-Dimorphic Sand Field Cricket, Gryllus Firmus, Anthony J. Zera, Susan Newman, David Berkheim, Christine Black, Lindsay Klug, Erica Crone

School of Biological Sciences: Faculty Publications

Cytoplasmic NADP+-isocitrate dehydrogenase (NADP+-IDH) has been purified and characterized, and its gene sequenced in many animal, plant, and yeast species. However, much less information is available on this enzyme-gene in insects. As a first step in investigating the biochemical and molecular mechanisms by which NADP+-IDH contributes to adaptations for flight vs. reproduction in insects, the enzyme was purified to homogeneity in the wing-dimorphic cricket, Gryllus firmus, characterized, and its corresponding gene sequenced. Using a combination of polyethylene glycol precipitation, Cibacron-Blue affinity chromatography, and hydrophobic interaction chromatography the enzyme was purified 291-fold (7% yield; specific activity = 15.8 μmol NADPH/min/mg …