Life Sciences Commons^™

Lipid Metabolism During Bacterial Growth, Sporulation, And Germination: Differential Synthesis Of Individual Branched And Normal-Chain Fatty Acids During Spore Germination And Outgrowth Of Bacillus Thuringiensis, Kenneth W. Nickerson, Lee A. Bulla Jr., Timothy L. Mounts

Papers in Microbiology

The biosynthesis of individual branched- and normal-chain fatty acids during Bacillus thuringiensis spore germination and outgrowth was studied by comparing pulsed and continuous labeling of these fatty acids with [U- ¹⁴C ]acetate. The relative specific activity of each fatty acid varies with time as the cell progresses through outgrowth. However, fatty acid synthesis does occur in two distinct phases. Upon germination, acetate is incorporated only into the iso-isomers i-C₁₃, i-C₁₄, and i-C₁₆; no normal or anteiso synthesis occurs. Subsequent to T₃₀, the full complement of branched- and normal-chain homologues is formed …

Agricultural Experiment Station News December 1975

Agricultural Research Division: News and Annual Reports

CONTENTS:
FROM THE ACTING DIRECTOR'S DESK
PERSONNEL ACTIONS
GRANTS AND CONTRACTS
GENERAL NOTES
AGRICULTURAL EXPERIMENT STATION PUBLICATIONS – NOVEMBER 1975

The Prairie Naturalist Vol. 7, No.4. December 1975

The Prairie Naturalist

COMPARISON OF FLOWERING RECORDS FROM KANSAS AND NORTH DAKOTA ▪ O. A. Stevens

REPAIRED BONE BREAK IN A HOUSE SPARROW ▪ C. L. Cink and T. K. Broschat

A LARGE RACOON LITTER ▪ E. K. Fritzell and J. W. Matthews

CHRISTMAS BIRD COUNTS FOR NORTH DAKOTA –1975 ▪ R. N. Randall

BOOK REVIEW

Autumn Hawk Migrations ▪ P. B. Hofslund

Separation Of Spores And Parasporal Crystals Of Bacillus Thuringiensis In Gradients Of Certain X-Ray Contrasting Agentsseparation Of Spores And Parasporal Crystals Of Bacillus Thuringiensis In Gradients Of Certain X-Ray Contrasting Agents, Eugene S. Sharpe, Kenneth W. Nickerson, Lee A. Bulla Jr., John N. Aronson

Papers in Microbiology

Spores and parasporal crystals of Bacillus thuringiensis can be separated at moderate centrifugation speeds (10,000 to 12,000 rpm) in gradients of Renografin or sodium diatrizoate.

89th Annual Report Of The Nebraska Agricultural Experiment Station

Agricultural Research Division: News and Annual Reports

Contents:
Foreword
Administration and Staff
Nebraska Agricultural Experiment Station
Research Projects
Experiment Station Publications
Publications of the Staff
Report of Income and Expenditures

Management Of Four Alfalfa Varieties To Control Damage From Potato Leafhoppers, W. R. Kehr, R. L. Ogden, J. D. Kindler

Nebraska Agricultural Experiment Station: Historical Research Bulletins

The main purpose of this study was to obtain information on forage yield and quality of four alfalfa (Medicago sativa L.) varieties that differed in level of resistance to potato leafhopper (Empoasca fabae Harris) yellowing, when cut at three stages of growth in the second and third cuttings in field plots, with and without insecticide application. The stages of growth were bud, 1/10, and full bloom. Other purposes included study of the same varieties in field cages manually infested at 20, 40, and 60 adult leafhoppers/square yard, and in supplemental cuttings of field plots under high natural infestation …

Whole Issue Nebraska Bird Review (December 1975) 43(4)

Nebraska Bird Review

Table of Contents

Spring Waterfowl Migration in Lancaster County - 1970.................. 70

Merlin Nest in Nebraska ..................78

A Groove-billed Ani Seen Again in Nebraska.................. 79

A Brown Creeper's Nest in Nebraska.................. 80

First Pleistocene Record of the Golden Eagle from the Central Great Plains.................. 84

Notes.................. 84

Index of Volume XXX XIII ..................85

Contents Of Chlorophylls A And B In Chlorophyll-Deficient Mutants Of Sweetclover, James E. Specht, Francis A. Haskins, H. J. Gorz

Department of Agronomy and Horticulture: Faculty Publications

Leaf extracts from twelve chlorophyll-deficient mutants of sweetclover (Melilotus alba Desr.) were compared spectrophotometrically and chromatographically with extracts from normal plants to determine contents of chlorophylls a and b Total chlorophyll contents in cotyledons and leaves of all mutants were significantly lower than those in corresponding tissues of normal plants. Chlorophyll contents were generally higher in later emerging leaves of both normal and mutant plants. Chlorophyll alb ratios in first trifoliolate leaves were similar to normal in mutants ch₇ and ch₈, higher in mutants ch₈, ch₉, ch₁₀, and ch_{11 …}

Sciurus Richmondi, J. Knox Jones Jr., Hugh H. Genoways

University of Nebraska State Museum: Mammalogy Papers

Sciurus richmondi Nelson, 1898
Richmond's Squirrel

Sciurus richmondi Nelson, 1898:146. Type locality Escondido River, 50 mi. above Bluefields, Nicaragua (Nelson, 1899:100).

Context and Content
Order Rodentia, Family Sciuridae. The genus Sciurus is widely distributed in both the Old and New worlds. S. richmondi is a monotypic species closely related to S. granatensis; both species are members of the subgenus Guerlinguetus.

Dipodomys Phillipsii, J. Knox Jones Jr.

University of Nebraska State Museum: Mammalogy Papers

Dipodomys phillipsii Gray, 1841
Southern Banner-tailed Kangaroo Rat

Dipodomys phillipsii Gray, 1841:522. Type locality "near Real del Monte," Hidalgo.
Dipodomys ornutus Merriam, 1894:110. Type locality Berriozi-bal, Zacatecas.
Dipodomys perotensis Merriam, 1894:111. Type locality Perote, Veracruz.

Sturnira Thomasi, J. Knox Jones Jr., Hugh H. Genoways

University of Nebraska State Museum: Mammalogy Papers

Sturnira thomasi de la Torre and Schwartz, 1966
Guadeloupe Yellow-shouldered Bat

Sturnira thomasi de la Torre and Schwartz, 1%6:299. Type locality Sofaia, 1,200 ft., island of Guadeloupe, Lesser Antilles.

Context and Content: Order Chiroptera, Family Phyllostomatidae, Subfamily Stenoderminae. The genus Sturnira contains about 10 species and is confined to the tropical and subtropical regions of the New World. Sturnira thomasi is a monotypic species.

Additional Records Of The Stenodermine Bat, Sturnina Thomasi, From The Lesser Antillean Island Of Guadeloupe, Hugh H. Genoways, J. Knox Jones

University of Nebraska State Museum: Mammalogy Papers

Sturnira thomusi was named and described by de la Torre and Schwartz (Proceedings of the Biological Society of Washington 79: 297-303, 1966) on the basis of a single male from the island of Guadeloupe, Lesser Antilles, that was captured by Richard Thomas in 1963. The holotype of S. thomasi has until now been the only known specimen of this unique species. In late July of 1974, a field party from the Museum of Texas Tech University (supported by a grant from the National Science Foundation, GB-41105) collected bats on Guadeloupe and took four additional individuals of S. thomasi, all …

Agricultural Experiment Station News November 1975

Agricultural Research Division: News and Annual Reports

CONTENTS:
FROM THE ACTING DIRECTOR'S DESK
GREETINGS FROM THE FROLIKS
PERSONNEL ACTIONS
GRANTS AND CONTRACTS
GENERAL NOTES
NEBRASKA AGRICULTURAL EXPERIMENT STATION PUBLICATIONS – October 1975

Reevaluation Of Common Wheats From The Usda World Wheat Collection For Protein And Lysine Content, K. P. Vogel, V. A. Johnson, P. J. Mattern

Nebraska Agricultural Experiment Station: Historical Research Bulletins

The protein and lysine content of wheat can be altered by breeding only if genetic variability exists for these traits. Lysine is a nutritionally limiting amino acid in wheat protein. Protein and lysine analyses of the common wheats of the USDA World Wheat Collection indicated that variability does exist for these traits. Some of the variability for protein and lysine among wheats of the World Collection may not be genetic in origin, because all the 12,000 wheats analyzed were not grown in the same environment. The purpose of this study was to reevaluate selected lines from the World Wheat Collection …

Prevalence Of Toxoplasma Antibodies In Range Vs. Dairy Cattle From The Bitterroot Valley Of Montana, Franklin Sogandares-Bernal, A. A. Marchiondo, Donald W. Duszynski, J. K. Ward

Harold W. Manter Laboratory of Parasitology: Faculty and Staff Publications

"Natural" or unaltered foods have become a fad in recent years. This includes the drinking of unpasteurized cow's milk as well as that designated "Certified." This report is made to call attention to a high prevalence of antibodies to toxoplasma in dairy as compared with range cattle. Previous to this study, few papers have considered the breed or conditions of management of cattle sampled for toxoplasma antibodies (McIlwain, 1969, Arch Environ Health 19: 885-886; Vanderwagen et aI., 1974, J Am Vet Med Assoc 164: 1,034-1,037).

Agricultural Experiment Station News October 1975

Agricultural Research Division: News and Annual Reports

CONTENTS:
FROM THE ACTING DIRECTOR'S DESK
PERSONNEL ACTIONS
GRANTS AND CONTRACTS
GENERAL NOTES
AGRICULTURAL EXPERIMENT STATION PUBLICATIONS - September 1975
BULLETINS PRINTED

Farm, Ranch And Home Quarterly Institute Of Agriculture And Natural Resources, University Of Nebraska- Lincoln

Farm, Ranch and Home Quarterly

In this issue:

2 Remote Sensing

6 FARMER NEB on the air

8 Potassium, Magnesium in Panhandle Soils

9 Humans in Nutrition Studies

11 Annual Windbreaks Save Moisture

13 The Rural Radio Turn.On

14 Cow-Calf Management in the 1970's

15 Soil Fertility in the Sandhills

17 Value of Drought-Damaged Corn

20 The Time of Your Life

22 Keeping the Gypsy Moth Out of Nebraska

24 Manure: Long-Term Study

27 The University Dairy Herd

A Review Of The Genus Allassostomoides Stunkard 1924 (Trematoda: Paramphistomidae) With A Redescription Of A. Chelydrae (Maccallum 1919) Yamaguti 1958, Daniel R. Brooks

Harold W. Manter Laboratory of Parasitology: Faculty and Staff Publications

A redescription of Allassostomoides chelydrae and a review of the genus is presented, based on new collections of A. chelydrae and A. parvus from Nebraska, and examination of all type material relating to the genus. A. chelydrae, A. parvus, and A. louisianaensis are considered valid species. New host records for A. chelydrae are Bufo americanus, Rana catesbeiana, Chrysemys picta, and Graptemys pseudogeographica.

Isolation And Identification Of Mycoplasma Agalactiae Subsp. Bovis From Arthritic Cattle In Iowa And Nebraska, O.H. V. Stalheim, S.S. Stone

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Two strains of Mycoplasma were isolated from synovial fluids of arthritic feeder cattle and were identified as Mycoplasma agalactiae subsp. bovis by growth inhibition and fluorescent antibody tests. The strains (Iowa 1136 and Nebraska 2) could not be distinguished from known strains (Donetta and California 01) by immunoelectrophoresis or by agar gel precipitation.

Agricultural Experiment Station News September 1975

Agricultural Research Division: News and Annual Reports

CONTENTS:
FROM THE DIRECTORS OFFICE
PERSONNEL ACTIONS
GRANTS AND CONTRACTS
GENERAL NOTES
AGRICULTURAL EXPERIMENT STATION PUBLICATIONS – August 1975
BULLETINS PRINTED

The Prairie Naturalist Vol. 7 , Nos. 2 And 3. June-September, 1975

The Prairie Naturalist

VASCULAR FLORA OF EMMONS COUNTY, NORTH DAKOTA ▪ R. P Williams and W. T. Barker

NOTES

Unusual Nesting Site of Barn Swallows ▪ W. A. Buresh

Summer Tanager in Burleigh County, North Dakota ▪ W. A. Buresh

Additional Record of Summer Tanager in North Dakota ▪ J. F. Cassel

Snowy Owl in McHenry County, North Dakota, in May ▪ W. A. Buresh

Seedling Growth Of Three Switchgrass Strains, L. J. Perry Jr., Lowell E. Moser

Department of Agronomy and Horticulture: Faculty Publications

Seedlings of Pathfinder, Nebr. 28, and experimental ey switchgrass (Panicum virgatum L.) strains were grown in a growth chamber and harvested 1, 2, 3, 4, 6, 8, and 10 weeks following emergence for detection of seedling growth differences among strains. Leaf areas and dry weights of leaf blade and stem axis (stem and leaf sheath) generally increased significantly with each harvest from 4 to 10 weeks. Stem axis and leaf blade dry weights were significantly greater with Pathfinder and ey, respectively, than with Nebr. 28. Final leaf area was significantly greater with ey than with the other strains. Thus, …

Buquinolate As A Preventive Drug To Control Microsporidosis In The Blue Crab, Robin M. Overstreet

Harold W. Manter Laboratory of Parasitology: Faculty and Staff Publications

When administered a single meal containing spores of Nosema michaelis and buquinolate, specimens of the blue crab, Callinectes sapidus, were less likely to acquire spore-ridden muscle tissue than individuals not allowed the drug. Those crabs presented the drug 48 hr preceding or following the introduction of spores also exhibited minimal incidence of infection. Even after 2 mo, spores were not observed in the musculature of most crabs.

Whole Issue Nebraska Bird Review (September 1975) 43(3)

Nebraska Bird Review

TABLE OF CONTENTS

1975 (Fiftieth) Spring Migration and Occurrence Report ... 46

Young Mountain Plover Seen in Kimball County ... 54

Notes ... 57

A Sutton's Warbler? ... 62

Scott's Oriole Reported ... 64

1975 Fall Field Day ... 66

A Junco Is a Junco Is a Junco ... 67

Pollution Of Surface Irrigation Waters By Plant Pathogenic Organisms, James R. Steadman, C. R. Maier, H. F. Schwartz, E. D. Kerr

Department of Plant Pathology: Faculty Publications

Systematic sampling of waterways and irrigation runoff from agricultural lands in the North Platte Project of Nebraska in July and August of 1972–1974 demonstrated that phytopathogenic organisms were disseminated. The organisms monitored included the bean common blight bacterium Xanthomonas phaseoli, the bean white mold fungus Whetzelinia sclerotiorum and various nematodes. Although many types of nematodes often were recovered from irrigation water, Heterodera sp. cysts which cause significant disease problems in the valley were found infrequently. Patterns of movement of the bacterial and fungal organisms were correlated with previous or current season infection of bean plants. The short-term survival of X. …

Agricultural Experiment Station News August 1975

Agricultural Research Division: News and Annual Reports

CONTENTS:
FROM THE ACTING DIRECTOR'S DESK
PERSONNEL ACTIONS
GRANTS AND CONTRACTS
GENERAL NOTES
NEBRASKA AGRICULTURAL EXPERIMENT STATION PUBLICATIONS - July 1975
BULLETINS PRINTED

Lipid Metabolism During Bacterial Growth, Sporulation, And Germination: An Obligate Nutritional Requirement In Bacillus Thuringiensis For Compounds That Stimulate Fatty Acid Synthesis, Kenneth W. Nickerson, Lee A. Bulla Jr.

Papers in Microbiology

The regulation of fatty acid biosynthesis by compounds that are required for growth of Bacillus thuringiensis was investigated using an in vivo assay developed to measure fatty acid synthesis in germinating spores. A minimal glucoseammonium- salts medium does not support growth even though previous radiorespirometric studies have shown B. thuringiensis to possess intact tricarboxylic acid and Embden-Meyerhof-Parnas pathways. Abundant growth does occur, however, when this medium is supplemented with either glutamate, aspartate, citrate, thiosulfate, cystine, or ethylenediaminetetraacetic acid. Cells held under non-growing conditions incorporate acetate into fatty acids; fatty acid synthesis is stimulated by the compounds that permit growth. These …

Physical And Chemical Properties Of Outdoor Beef Cattle Feedlot Runoff, C. B. Gilbertson, J. R. Ellis, J. A. Nienaber, T. M. Mccalla, T. J. Klopfenstein

Nebraska Agricultural Experiment Station: Historical Research Bulletins

Within the past decade, emphasis has been placed on the quality of our environment. Beef cattle feedlots have been singled out as a significant source of environmental pollution. Environmental protection provided by control facilities requires basic knowledge of the physical and chemical properties of feedlot runoff. This bulletin summarizes studies of physical and chemical properties of beef cattle feedlot runoff completed from August, 1968 through December, 1972.

An Economic Information Program For The U.S. Beef And Pork Sectors, N. A. Aulaqi, J. B. Hassler

Nebraska Agricultural Experiment Station: Historical Research Bulletins

Major objectives of the study were: first, to review present public data programs and to indicate areas of inefficient performance in the production and marketing of beef and pork that still exist under the present data system; second, to simulate an operational information service program for the beef and pork sectors based on programmed solutions for industry allocations and activities; and third, to outline possible implementation procedures for the information service programs and to indicate the role of each of the participants in making it successful.

Sire Comparisons For Holsteins In Mexico Versus The United States And Canada, R. E. Mcdowell, G. R. Wiggans, J. K. Camoens, L. Dale Van Vleck, D. G. St. Louis

Department of Animal Science: Faculty Publications

Henderson's mixed model procedure was used to determine sire comparisons for Holstein sires from the United States, Canada, or local bred in 48 Mexican herds. For sires with five or more daughters, 74% of the sires in artificial insemination from the United States had plus values compared to 34% from Canada, 44% from the local stud, and 40% for local bred sires. Genetic correlations with Mexican sire comparisons were Northeast Artificial Insemination Sire Comparison with >20 daughters in Mexico .86, with 10 to 20 daughters .71, United States Department of Agriculture (USDA) proof >10 daughters .81, and Canada Northeast Artificial …