Life Sciences Commons^™

Effects Of Fungal Biopesticide Doses On Mortality In Bumble Bees, Colton Burris

Undergraduate Research Symposium

Agricultural practices often include the use of pesticides to produce meaningful crop yields. While the pesticide may be used to target “pest” insects (e.g. thrips, aphids, whiteflies), it can also have negative effects on important pollinating insects such as bees. Neonicotinoid pesticides have been found responsible for widespread decline in bee biodiversity, and were even banned in Europe (Sgolastra et al. 2020) . Beauveria bassiana, sold as Botanigard, on the other hand, is a fungal biopesticide that is marketed to be a safer alternative for bees, and has been found to have other possibly harmful effects to the inner …

The Revolutionary St. Louis Insane Asylum, Julia Talbert

Undergraduate Research Symposium

Even with its grand structure on Arsenal Street towering over the city below, few residents of St. Louis and surrounding areas are aware of the grand history or even existence of the St. Louis Insane Asylum. The building is over 150 years old and was a place of hope, failure, strife, and empathy. The asylum had a large impact on St. Louis and provided revolutionary outlooks, unique perspectives, and curious therapies.

Steminism: Analyzing Factors That Improve Retention Of Women In Stem, Kira Carter, Jane Kelley, Jason Vasser-Elong, Rc Patterson

Dissertations

Our co-authored research ‘Steminism: Analyzing Factors That Improve Retention for Women as STEM Majors’ analyzed factors that contributed to the retention of women in science, technology, engineering, and mathematics (STEM) programs at Missouri University of Science & Technology (Missouri S&T). Women make up half of the US population, and while careers in (STEM) are an integral part of the US economy, women are underrepresented in these career fields. The purpose of our dissertation is to address the underrepresentation of women in STEM majors. Our methodology included homogeneous sampling to collect qualitative data. More specifically, we consulted with academic advisors and …

Studying The Genes And Conditions That Influence Root Development, Tessa Holtkamp, Hannah Ordonez Webb

Undergraduate Research Symposium

Root development in plants is essential for their survival and understanding how hormones influence their development can explain how plants grow under different circumstances. Researching how Indole-3-butyric acid (IBA), a hormone that induces root production, affects the plant model Arabidopsis thaliana helps explain the hormone's effect in agricultural crop systems. To understand root pathways, we performed assays on mutant lines of Arabidopsis by growing plants on varying concentrations of IBA. For wild-type and mutant lines, phenotyping experiments like branching of roots, lengths of stems, and root length were conducted along with PCR and restriction digest genotyping experiments to compare their …

Effects Of Beauveria Bassiana On The Mortality And Thorax Width Of Bombus Impatiens Colonies, Bri Petty

Undergraduate Research Symposium

Pesticides are widely used to protect against unwanted insects, but risk assessments of the effects on pollinators have traditionally only been applied on an individual level, not a population level. This has allowed many pesticides to be approved without the knowledge of sub-lethal effects, such as life history characteristics and population structure to name a few. The biopesticide BotaniGard, containing the entomopathogenic fungus Beauveria bassiana, has previously been thought to be safe for non-targeted insects, but recent studies have found it to have significant negative effects on pollinators. Such negative effects can include decrease in longevity and odorant responsiveness with …

Comparing Sucrose Preference Between Colonies Of Bombus Impatiens, Kevin R. Corrigan, Becky Hansis-O'Niell, Aimee Sue Dunlap

Undergraduate Research Symposium

Current studies in nectar concentration preference generally take the form of a static laboratory experiment in which a multitude of feeders provide a constant source of nectar in differing concentrations and data is collected on which one is preferred most by bees. Studies like these are important because they seek to gain an understanding of bee foraging patterns. Having a strong biological understanding of these organisms better prepares us to combat issues regarding conservation should they occur. Here, we conduct an analysis on the foraging patterns of two colonies of Bombus impatiens (B. Impatiens) with data gathered from fixed ratio …

Animal Conservation In St. Louis, Kate O'Sullivan

Undergraduate Research Symposium

St. Louis has a multitude of organizations involved in the natural sciences. But how many of them actually contribute towards animal conservation? The St. Louis Zoo is an organization that focuses a lot of its effort on presentation, so how does that impact the funds that go towards actually saving the animals? I plan to dive into the different animal-based organizations in St. Louis and its surrounding areas, as well as discuss the positives and negatives of each organization. Furthermore, I will provide examples from several sources that I have been reviewing all year to support my claims. I plan …

Surveys Of Aphonopelma Hentzi In Missouri: Conservation Efforts Through Population, Genetics, And Habitat Studies, Anderson B. Spencer Mr., Becky Hansis-O'Niell

Undergraduate Research Symposium

Aphonopelma hentzi and other tarantula species are greatly understudied organisms. In the Missouri regions there is little knowledge on their mating patterns, gene diversity, or population sizes. The focal glades in this experiment display regional fragmentation, which could prevent tarantulas from traveling and mating between regions of their glades. Due to the lack of knowledge surrounding them, methods for determining gene diversity are harmful to the organism. Our recent work has shown that it is possible to extract genetic information from the molts of burrowing tarantulas. This will allow us to safely determine the lineages and interbreeding patterns of the …

The Effect Of Caffeine On Bee Behavior: A Progressive Ratio Study, Kayle Cohen, Becky Hansis-O'Neill, Aimee Dunlap Dr

Undergraduate Research Symposium

This presentation focuses on the effect of caffeine on bee behavior using behavioral pharmacology methodologies. Researchers trained bumblebees to drink out of artificial flowers, then administered sucrose nectar or caffeinated sucrose nectar during a schedule of progressive and fixed ratios. The finding suggests that caffeine did increase the number of rewards during the fixed ratio, but not in the progressive ratio. However, research is still ongoing as bees continue to be tested..

Finding Gene Candidates That Interact With Mara To Control Hila Expression In Salmonella Enterica, Kylee Hempel

Undergraduate Research Symposium

Salmonella is a well-known pathogen that is linked to food born illnesses common around the world. This pathogen can cause symptoms such as fever, diarrhea, and chills in the host. One of the mechanisms Salmonella uses to infect its host’s epithelial cells deals with the S. typhimurium pathogenicity island 1 (SPI-1). Expression of SPI-1 is activated in response to environmental signals that correlate with the ileum of the small intestine (low oxygen, high osmolarity, and neutral pH). HilA is the central activator of the SPI-1 complex, and when the transcription factor, MarA, is over-expressed in Salmonella, hilA transcription is …

Recovering Ancient Dna Using The Polymerase Chain Reaction, Rose Jennings

Undergraduate Research Symposium

Investigations into aDNA offer a window into the past that modern DNA and paleontological studies alone cannot provide and help address the evolution and connections between hominids, domestication timelines, the analysis of populations over time, and general diversity. Progress in aDNA research has been inherently technology-driven, with modern molecular biology methods, such as the inventions of Polymerase Chain Reaction (PCR) and Next Generation Sequencing (NGS), substantially increasing the analysis possibilities of aDNA. My research this semester has taken me along two parallel paths of investigation: literary research into aDNA and practical exposure to the laboratory techniques used in its analysis. …

Investigation Of Alzheimer’S Amyloid-Β Protein Aggregation With A New Fluorescent Dye., Emma Alberty

Undergraduate Research Symposium

Alzheimer’s Disease (AD) is the most common form of dementia characterized by the impairment of at least two brain functions such as memory loss and judgement. AD is a progressive illness that can last as many as 20 years. AD is largely considered to be caused by the formation of extracellular amyloid plaques and intracellular neurofibrillary tangles. A better understanding of the structure and function of these plaques may lead to clearer understanding of the disease. To analyze amyloid plaques, aggregation assays are often used. During these assays we begin with monomer and place the sample in biological conditions to …

Binding Interactions Of Biologically Relevant Molecules Studied Using Surface-Modified And Nanostructured Surfaces, Palak Sondhi

Dissertations

This research focuses on the field of surface nanobioscience, wherein different nanosurfaces that will be used as working electrodes in the electrochemical cell are manufactured and surface modified to understand the critical binding interactions between biologically significant molecules like proteins, carbohydrates, small drug molecules, and glycoproteins. This research is essential if we are to determine whether a synthetic molecule can serve as a therapeutic candidate or diagnose a disease in its early stages. In order to fully understand the binding interactions, the study begins with defining some of the fundamental concepts, principles, and analytical tools for biosensing.

Afterwards, we addressed …

Assessing Threats To Plant Populations: Linking Pollinator Differences To Patterns Of Plant Fitness And Population Genetics, Rieka Yu

Dissertations

Land use change is a major driver of biodiversity loss and consequently has led to the loss of genetic diversity in many plant populations due to declines in population sizes and an increase in spatial isolation. However, not all plant populations respond similarly to land-use change, suggesting there are additional mechanisms mediating plant population genetic patterns. Here, I examine the role of pollinators as this mediating factor. In Chapter 1 I conducted a meta-analysis to investigate how different types of pollinators drive changes in gene flow for plant populations in disturbed habitats, finding that different types of pollinators mediate different …

Phylogeny And Taxonomy Of Genus Physaria In North America, Binoshi Hettihewa

Theses

Physaria is a genus of ~108 species belonging to family Brassicaceae that is predominantly distributed in Western North America, but one species occurs in Arctic Russia and Northern Canada and several species occur in South America. Regardless of the vast number of species in the genus, the genus lacks a well-resolved phylogeny representing many taxa, partially because phylogeny reconstruction is complicated by the fact that many species of Physaria vary in chromosome numbers and ploidy levels. In chapter 1, we review how polyploids are formed and become established and summarize what is known about variation in chromosome number and ploidy …

Probing Amyloid-Beta Protein Structure And Dynamics With A Selective Antibody, Shikha Grover

Dissertations

Alzheimer’s disease (AD) is a progressive neurodegenerative disorder. The AD brain is characterized by significant neuronal loss and accumulation of insoluble fibrillar amyloid-β protein (Aβ) plaques and tau protein neurofibrillary tangles in the brain. However, over the last decade, many studies have shown that the neurodegenerative effect of Aβ may in fact be caused by various soluble oligomeric forms as opposed to the insoluble fibrils. Furthermore, the data suggest that a pre-fibrillar aggregated form, termed protofibrils, mediates direct neurotoxicity, and triggers a robust neuroinflammatory response.

Antibodies targeting the various conformation of Aβ are important therapeutic agents to prevent the progression …

Genetic And Environmental Drivers Of Microbial Colonization In Wild Birds, Sage Rohrer

Dissertations

Wild birds are teeming with microorganisms, ranging from commensal bacteria to eukaryotic parasites. These microbes impact host health in diverse ways; some long-term residents may aid digestion or provide immune system training, while others at times may induce disease. However, the factors driving the varying colonization patterns seen across taxa are still not fully understood, and wild avian populations are particularly understudied when compared to mammalian or domesticated systems. This work examines the relative importance of genetic and environmental factors driving microbiome composition and function in wild birds. We collected fecal and blood samples from Galapagos penguins (Spheniscus mendiculus …

The Effects Of Floral And Social Information On Bumblebee Forager Learning And Memory, Avery Hume Baker

Theses

Bumblebees rely on information gathered from their environment to make the best choices they can when foraging for pollen and nectar. The type of information gathered should influence how a bee learns and remembers it, but other factors such as the size of the bee’s brain may also play a role in the learning and remembering process. While social information learned from other organisms and information gathered directly from flowers can each be used alone to improve both the efficiency with which a bee learns to forage from a flower and how accurately and how long the bee remembers these …

Taxonomic Revision And Morphometric Analysis Of Selected Anthurium (Araceae) Species From Bolivia And Peru, Daniel M. Tarazona Ocana

Theses

The genus Anthurium (Araceae) consists of around 1,200 species distributed in the Neotropics, particularly in Central and South America. The montane forests in the eastern flank of the Andes, ranging from northern Ecuador to central Bolivia; hereafter, the East Andes Gradient region, are particularly rich in terms of species diversity, and include an understudied group of Anthurium species endemic to the region. Within this group of species, some taxa are difficult to distinguish from each other mainly due to the lack of identification keys, incomplete species descriptions and unknown synonyms. In this study, a combination of traditional taxonomic techniques and …

Ferrocenium Salt Aided Substitution Reactions And Synthesis Of Glycosylated Curcumin Derivatives, Deva Saroja Talasila

Dissertations

Organic synthesis has been significantly advanced with the employment of transition metal complexes. The discovery of transition metal catalysts provided the synthetic community with powerful tools for accelerating reactions and making them more selective and efficient. Many chemical reactions do not happen without a catalyst.

Iron-based catalysts have several advantages for the chemical industry because it is a non-toxic and ecologically friendly metal. Our group previously found that ferrocenium cations with a 3+ oxidation state of iron-catalyzed propargylic substitution reactions at low temperatures. The sandwich structure of ferrocenes allows substituents to be introduced on the cyclopentadienyl rings, which allows for …

The Ecology And Evolution Of Species Rarity In Oaks (Quercus Spp.), Yingtong Wu

Dissertations

Rare species are susceptible to extinction due to ecological and genetic factors. Understanding the distribution, ecology, and evolution of rare species can provide useful information for effective conservation. To investigate species rarity, this dissertation focuses on a species-rich and ecologically diverse genus, Quercus (oaks). In Chapter 1, I aimed to understand how interactions between hosts and soil microbes contribute to habitat restriction in oak species. I performed a soil inoculum experiment on two pairs of sister oak species that show habitat divergence. I found that host-specific soil microbes contribute to habitat divergence and exclusion among sister species of oaks, but …

The Effects Of Prescribed Fire On Ant-Mediated Seed Dispersal In Missouri, Eva M. Colberg

Dissertations

Many aspects of animal-mediated seed dispersal are vulnerable to disturbance, including partner identity and dispersal quantity and quality. This dissertation explores ant-mediated seed dispersal of the herb Sanguinaria canadensis in Missouri Ozark oak forests, where prescribed fire is a common land management tool. In Chapter 1, I test the definition of a keystone seed disperser using absolute and relative contributions of different ant species to the quantity and distance of seed dispersal, based on field observations of S. canadensis seed dispersal. I demonstrate that the ant Aphaenogaster rudis is better described as a numerically dominant rather than keystone seed disperser, …

Immune Cell-Protein Interactions In Alzheimer’S Disease, Kapur Dhami

Dissertations

Misfolded protein aggregates are one of the significant contributing factors in many neurogenerative diseases including Alzheimer’s disease, Parkinson’s disease, Huntington’s disease, and others. My PhD research project was to study various aspects of amyloid-β peptide, a 40-42-residue peptide and the primary component of the senile plaques found in Alzheimer’s disease (AD) brains. One objective of my research was to purify and characterize the intermediate Aβ42 species using an array of biophysical techniques like size exclusion chromatography, fluorescence assays, circular dichroism, cellular assays, and protein assays. The other major research thrust of my project was to study the role of microglial …

Mechanism Of Sila- And Germafluorenes For Biological Applications, Shelby Jarrett

Dissertations

2,7-disubstituted silafluorenes and germafluorenes, originally designed for OLED applications, are a class of fluorescent dyes that have gained recent interest as probes for bioimaging and as biosensors to monitor cellular dynamics and interactions. Desirable biological probes absorb in the visible region, have high extinction coefficients, high quantum yield and excellent photostability. Here, their spectral properties are investigated under aqueous conditions for relevant biological applications. These molecules display intense blue fluorescence in the solid state and in solution, have high extinction coefficients, and exhibit appreciable solubility in aqueous solution. To better understand potential applications, the mechanism of fluorescence was investigated. It …

Effects Of Forest Restoration On The Recovery Of Dead Wood, Associated Arthropods, And Insect-Mediated Wood Decomposition, Estefania Fernandez Barrancos

Dissertations

Dead wood represents 8% of terrestrial carbon stocks and is an important source of habitat and food for decomposer and non-decomposer arthropods. However, anthropogenic disturbance reduces the amount of dead wood, putting at risk the presence of a habitat that is essential for many arthropods and other organisms that rely on it for food and shelter. Forest restoration aims to assist the recovery of ecosystems that have been damaged or destroyed and could be a means to recover both dead wood and its associated arthropod communities. This doctoral dissertation lies at the intersection of climate change, biodiversity loss and ecological …

Critical Corridor: Survey Of Predator Occurrence And Habitat Use In A Threatened Rainforest Protected Area: Comatsa-Sud And Marojejy National Park, Madagascar, Patrick H. Ross

Theses

Madagascar is a nation praised for its floral and faunal endemism and biodiversity. Among the island nation’s most emblematic fauna are its native mammalian carnivores; they are members of the threatened and endemic Eupleridae family. The Corridor of Marojejy – Anjanaharibe Sud – Tsaratanana (COMATSA) is a system of forest protected areas in which three large protected areas are connected in northern Madagascar: Tsaratanana Reserve, Anjanaharibe Sud Special Reserve and Marojejy National Park which is also a UNESCO World Heritage site. COMATSA is a newly protected and currently threatened corridor system that lacks detailed surveys and assessments of its native …

The Effects Of Floral Attributes And Conspecifics On Bumble Bee Forager Memory, Lucas Lauter, Tiffany Dinh

Undergraduate Research Symposium

What do bees remember about flowers? These memories are important for both bees and flowers. The bees have better foraging success and gain more nectar and pollen from flowers when they remember the most rewarding flower types. More memorable flowers will be visited more frequently, resulting in more successful pollination for the plant. At the same time, bees can also learn about flowers from other bees and may remember this information differently. We are training and testing three floral cues and a single social cue to see how the different types of cues affect their learning and memory of rewarding …

Competition Between Honey Bees (Apis Mellifera) And Native Bees: An Investigation In Urban Community Gardens In St. Louis, Missouri, Owen Ireton

Undergraduate Research Symposium

The European honey bee (Apis mellifera), is considered one of the worlds more important pollinator species and is often the focal species for bee conservation. However, lesser known native bees are equally, if not more valuable for pollination services. Native bees are better pollinators for native crop plants, but often must compete with honeybees for floral resources. There has been an increase in support for including honeybee hives in private and public spaces, but recent studies suggest that interactions between resident native bees and introduced honeybees can have long lasting and detrimental effects on population persistence. These interactions have been …

Analysis Of Soxs In S. Typhimurium By Transposon Mutagenesis, Joel Hanns, Brenda Pratte, Lon Chubiz Phd, Lauren Daugherty

Undergraduate Research Symposium

The mar-sox-rob regulon has been implicated in transcriptional regulation of several stress responses, such efflux of antibiotics, enzymes that break down reactive oxygen species, repression of biofilm formation, or repression of motility through downregulation of flagellar expression. This system is conserved among enteric bacteria and has been studied in species, such as E. coli and S. typhimurium. Some of these mechanisms can be costly and slow cell growth while increasing the probability of survival through tolerance of toxic environments. SoxS works in coordination with SoxR to respond to redox stress encountered by the cell. Interestingly, the overexpression of SoxS …

Resolving The Repression Pathway Of Virulence Gene Hila In Salmonella, Alexandra King, Lon Chubiz Phd, Brenda Pratte, Lauren Daugherty

Undergraduate Research Symposium

Salmonella is a relatively abundant, virulent species of bacteria that is most known for spreading gastrointestinal diseases through food. These illnesses result in approximately 1.35 million infections, including over 25,000 hospitalizations each year, in the U.S. alone (CDC.gov). As antibiotic resistance becomes an increasingly urgent public health problem, the importance of developing alternative treatment methods is only becoming more crucial. One of the genes responsible for this virulence is known as hilA. HilA is the main transcriptional regulator of Salmonella Pathogenicity Island-1 gene (UniProt). SPI-1 plays an important role in the invasion of Salmonella into epithelial cells. The proteins encoded …