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Disorganization Of Cortical Microtubules Stimulates Tangential Expansion And Reduces The Uniformity Of Cellulose Microfibril Alignment Among Cells In The Root Of Arabidopsis Thaliana, Tobias Baskin, G.T.S. Beemster, J.E. Judy-March, F. Marga
Disorganization Of Cortical Microtubules Stimulates Tangential Expansion And Reduces The Uniformity Of Cellulose Microfibril Alignment Among Cells In The Root Of Arabidopsis Thaliana, Tobias Baskin, G.T.S. Beemster, J.E. Judy-March, F. Marga
Tobias Baskin
To test the role of cortical microtubules in aligning cellulose microfibrils and controlling anisotropic expansion, we exposed Arabidopsis thaliana roots to moderate levels of the microtubule inhibitor, oryzalin. After 2 d of treatment, roots grow at approximately steady state. At that time, the spatial profiles of relative expansion rate in length and diameter were quantified, and roots were cryofixed, freeze-substituted, embedded in plastic, and sectioned. The angular distribution of microtubules as a function of distance from the tip was quantified from antitubulin immunofluorescence images. In alternate sections, the overall amount of alignment among microfibrils and their mean orientation as a …