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In Vivo Dose-Response Of Insects To Hz-2v Infection, John P. Burand, Christopher P. Rallis Dec 2004

In Vivo Dose-Response Of Insects To Hz-2v Infection, John P. Burand, Christopher P. Rallis

John Burand

Background Hz-2V infection of female Helicoverpa zea moths is manifested as insects that are either sterile "agonadal" individuals with malformed reproductive tissues or fertile asymptomatic carriers which are capable of transmitting virus on to their progeny. Virus infected progeny arising from eggs laid by asymptomatic carrier females may themselves be either sterile agonadals or asymptomatic carriers. Results By injecting virus into female moths, a correlation was established between virus doses administered to the females and the levels of resulting asymptomatic and sterile progeny. Conclusions The results of these experiments indicate that high virus doses produced a higher level of agonadal …


Allele Specific Synthetic Lethality Between Pric And Dnaats Alleles At The Permissive Temperature Of 30°C In E. Coli K-12, Steven Sandler, Tania Hinds Dec 2004

Allele Specific Synthetic Lethality Between Pric And Dnaats Alleles At The Permissive Temperature Of 30°C In E. Coli K-12, Steven Sandler, Tania Hinds

Steven Sandler

Background DnaA is an essential protein in the regulation and initiation of DNA replication in many bacteria. It forms a protein-DNA complex at oriC to which DnaC loads DnaB. DNA replication forks initiated at oriC by DnaA can collapse on route to the terminus for a variety of reasons. PriA, PriB, PriC, DnaT, Rep and DnaC form multiple pathways to restart repaired replication forks. DnaC809 and dnaC809,820 are suppressors of priA2::kan mutant phenotypes. The former requires PriC and Rep while the latter is independent of them. RnhA339::cat mutations allow DnaA-independent initiation of DNA replication. Results It is shown herein that …


Dna Microarray Analysis Of Nitrogen Fixation And Fe(Iii) Reduction In Geobacter Sulfurreducens, Jennifer Webster, Barbara A. Methé, Kelly P. Nevin, Jessica E. Butler, Derek Lovley Dec 2004

Dna Microarray Analysis Of Nitrogen Fixation And Fe(Iii) Reduction In Geobacter Sulfurreducens, Jennifer Webster, Barbara A. Methé, Kelly P. Nevin, Jessica E. Butler, Derek Lovley

Derek Lovley

A DNA microarray representing the genome of Geobacter sulfurreducens was constructed for use in global gene expression profiling of cells under steady-state conditions with acetate as the electron donor and Fe(III) or fumarate as the electron acceptor. Reproducible differences in transcript levels were also observed in comparisons between cells grown with ammonia and those fixing atmospheric nitrogen. There was a high correlation between changes in transcript levels determined with microarray analyses and an evaluation of a subset of the genome with quantitative PCR. As expected, cells required to fix nitrogen had higher levels of transcripts of genes associated with nitrogen …


In Situ Expression Of Nigd In Geobacteraceae In Subsurface Sediments, Dawn E. Holmes, Kelly P. Nevin, Derek Lovley Dec 2004

In Situ Expression Of Nigd In Geobacteraceae In Subsurface Sediments, Dawn E. Holmes, Kelly P. Nevin, Derek Lovley

Kelly Nevin

In order to determine whether the metabolic state of Geobacteraceae involved in bioremediation of subsurface sediments might be inferred from levels of mRNA for key genes, in situ expression of nifD, a highly conserved gene involved in nitrogen fixation, was investigated. When Geobacter sulfurreducens was grown without a source of fixed nitrogen in chemostats with acetate provided as the limiting electron donor and Fe(III) as the electron acceptor, levels of nifD transcripts were 4 to 5 orders of magnitude higher than in chemostat cultures provided with ammonium. In contrast, the number of transcripts of recA and the 16S rRNA gene …


Evidence For Involvement Of An Electron Shuttle In Electricity Generation By Geothrix Fermentans, Daiel R. Bond, Derek Lovley Nov 2004

Evidence For Involvement Of An Electron Shuttle In Electricity Generation By Geothrix Fermentans, Daiel R. Bond, Derek Lovley

Derek Lovley

In experiments performed using graphite electrodes poised by a potentiostat (+200 mV versus Ag/AgCl) or in a microbial fuel cell (with oxygen as the electron acceptor), the Fe(III)-reducing organism Geothrix fermentans conserved energy to support growth by coupling the complete oxidation of acetate to reduction of a graphite electrode. Other organic compounds, such as lactate, malate, propionate, and succinate as well as components of peptone and yeast extract, were utilized for electricity production. However, electrical characteristics and the results of shuttling assays indicated that unlike previously described electrode-reducing microorganisms, G. fermentans produced a compound that promoted electrode reduction. This is …


The Unpredictable Past Of Plasmodium Vivax Revealed In Its Genome, Stephen M. Rich Oct 2004

The Unpredictable Past Of Plasmodium Vivax Revealed In Its Genome, Stephen M. Rich

Stephen M. Rich

Until quite recently, very little information has been available about the genome content and structure of parasitic protozoa. This inadequacy has been rectified by the advent of high-throughput strategies that permit sequencing of whole genomes and enhanced computational capacities that render this information tractable. The current list of complete or near-complete genomes includes some of the greatest scourges of humans and their domesticated companions. Among these miscreants are several members of the genus Plasmodium, the agents of malaria. Scores of species of Plasmodium have been described and comprise pathogens of every major group of terrestrial vertebrates. At present, no less …


Resistance Of Solid-Phase U(Vi) To Microbial Reduction During In Situ Bioremediation Of Uranium-Contaminated Groundwater, Irene Ortiz-Bernad, Robert T. Anderson, Helen A. Vrionis, Derek Lovley Jul 2004

Resistance Of Solid-Phase U(Vi) To Microbial Reduction During In Situ Bioremediation Of Uranium-Contaminated Groundwater, Irene Ortiz-Bernad, Robert T. Anderson, Helen A. Vrionis, Derek Lovley

Derek Lovley

Speciation of solid-phase uranium in uranium-contaminated subsurface sediments undergoing uranium bioremediation demonstrated that although microbial reduction of soluble U(VI) readily immobilized uranium as U(IV), a substantial portion of the U(VI) in the aquifer was strongly associated with the sediments and was not microbially reducible. These results have important implications for in situ uranium bioremediation strategies.


Caveolin-2 Associates With Intracellular Chlamydial Inclusions Independently Of Caveolin-1, Wilmore C. Webley, Leonard C. Norkin, Elizabeth S. Stuart Jul 2004

Caveolin-2 Associates With Intracellular Chlamydial Inclusions Independently Of Caveolin-1, Wilmore C. Webley, Leonard C. Norkin, Elizabeth S. Stuart

Wilmore C Webley

Background Lipid raft domains form in plasma membranes of eukaryotic cells by the tight packing of glycosphingolipids and cholesterol. Caveolae are invaginated structures that form in lipid raft domains when the protein caveolin-1 is expressed. The Chlamydiaceae are obligate intracellular bacterial pathogens that replicate entirely within inclusions that develop from the phagocytic vacuoles in which they enter. We recently found that host cell caveolin-1 is associated with the intracellular vacuoles and inclusions of some chlamydial strains and species, and that entry of those strains depends on intact lipid raft domains. Caveolin-2 is another member of the caveolin family of proteins …


In Situ Expression Of Nigd In Geobacteraceae In Subsurface Sediments, Dawn E. Holmes, Kelly P. Nevin, Derek Lovley Jul 2004

In Situ Expression Of Nigd In Geobacteraceae In Subsurface Sediments, Dawn E. Holmes, Kelly P. Nevin, Derek Lovley

Derek Lovley

In order to determine whether the metabolic state of Geobacteraceae involved in bioremediation of subsurface sediments might be inferred from levels of mRNA for key genes, in situ expression of nifD, a highly conserved gene involved in nitrogen fixation, was investigated. When Geobacter sulfurreducens was grown without a source of fixed nitrogen in chemostats with acetate provided as the limiting electron donor and Fe(III) as the electron acceptor, levels of nifD transcripts were 4 to 5 orders of magnitude higher than in chemostat cultures provided with ammonium. In contrast, the number of transcripts of recA and the 16S rRNA gene …


Potential Role Of A Novel Psychrotolerant Member Of The Family Geobacteraceae, Geopsychrobacter Electrodiphilus Gen.Nov.,Sp.Nov.,In Electricity Production By A Marine Sediment Fuel Cell, Dawn E. Holmes, Julie S. Nicoll, Daniel R. Bond, Derek Lovley Jun 2004

Potential Role Of A Novel Psychrotolerant Member Of The Family Geobacteraceae, Geopsychrobacter Electrodiphilus Gen.Nov.,Sp.Nov.,In Electricity Production By A Marine Sediment Fuel Cell, Dawn E. Holmes, Julie S. Nicoll, Daniel R. Bond, Derek Lovley

Derek Lovley

Previous studies have shown that members of the family Geobacteraceae that attach to the anodes of sediment fuel cells are directly involved in harvesting electricity by oxidizing organic compounds to carbon dioxide and transferring the electrons to the anode. In order to learn more about this process, microorganisms from the anode surface of a marine sediment fuel cell were enriched and isolated with Fe(III) oxide. Two unique marine isolates were recovered, strains A1T and A2. They are gram-negative, nonmotile rods, with abundant c-type cytochromes. Phylogenetic analysis of the 16S rRNA, recA, gyrB, fusA, rpoB, and nifD genes indicated that strains …


Attracting Antagonists: Does Floral Nectar Increase Leaf Herbivory?, Lynn Adler, Judith L. Bronstein Jun 2004

Attracting Antagonists: Does Floral Nectar Increase Leaf Herbivory?, Lynn Adler, Judith L. Bronstein

Lynn Adler

Traits that are attractive to mutualists may also attract antagonists, resulting in conflicting selection pressures. Here we develop the idea that increased floral nectar production can, in some cases, increase herbivory. In these situations, selection for increased nectar production to attract pollinators may be constrained by a linked cost of herbivore attraction. In support of this hypothesis, we report that experimentally supplementing nectar rewards in Datura stramonium led to increased oviposition by Manduca sexta, a sphingid moth that pollinates flowers, but whose larvae feed on leaf tissue. We speculate that nectar composition may provide information about plant nutritional status or …


Direct Correlation Between Rates Of Anaerobic Respiration And Levels Of Mrna For Key Respiratory Genees In Geobacter Sulfurreducens, Abraham Esteve-Núñez, Ching Leang, Kuk-Jeong Chin, Derek Lovley May 2004

Direct Correlation Between Rates Of Anaerobic Respiration And Levels Of Mrna For Key Respiratory Genees In Geobacter Sulfurreducens, Abraham Esteve-Núñez, Ching Leang, Kuk-Jeong Chin, Derek Lovley

Derek Lovley

The predominance of Geobacter species in environments in which Fe(III) reduction is important has suggested that Fe(III) reduction rates might be estimated in Geobacter-dominated environments by assessing in situ activity with molecular techniques. To determine whether mRNA levels of key respiratory genes might be correlated with respiration rates in Geobacter sulfurreducens, studies were conducted with fumarate as the electron acceptor and acetate as the limiting electron donor in anaerobic continuous cultures. Levels of mRNA for a fumarate reductase gene, frdA, quantified by real-time reverse transcription-PCR were directly correlated with fumarate reduction rates. In similar studies with Fe(III) as the electron …


Synergistic And Antagonistic Roles Of The Sonic Hedgehog N- And C-Terminal Lipids, Jianchi Feng, Bryan White, Oksana V. Tyurina, Burcu Guner, Theresa Larson, Hae Young Lee, Rolf O. Karlstrom, Jhumku D. Kohtz May 2004

Synergistic And Antagonistic Roles Of The Sonic Hedgehog N- And C-Terminal Lipids, Jianchi Feng, Bryan White, Oksana V. Tyurina, Burcu Guner, Theresa Larson, Hae Young Lee, Rolf O. Karlstrom, Jhumku D. Kohtz

Rolf O Karlstrom

The Shh protein contains both N-terminal and C-terminal lipids. The functional redundancy of these lipid moieties is presently unclear. Here, we compare the relative roles of the N- and C-terminal lipids in early rat striatal neuronal differentiation, membrane association and multimerization, and ventralizing activity in the zebrafish forebrain. We show that these lipid act synergistically in cell tethering and the formation of a large (L) multimer (669 kDa). However, the C-terminal lipid antagonizes the rat striatal neuronal differentiation-inducing activity of the N-terminal lipid. In addition, multimerization is required but not sufficient for the differentiation-inducing activity. Based on the presence of …


A Regulatory Code For Neurogenic Gene Expression In The Drosophila Embryo, Michele Markstein, Robert Zinzen, Peter Markstein, Ka Ping Yee, Albert Erives, Angela Stathopoulos, Michael Levine May 2004

A Regulatory Code For Neurogenic Gene Expression In The Drosophila Embryo, Michele Markstein, Robert Zinzen, Peter Markstein, Ka Ping Yee, Albert Erives, Angela Stathopoulos, Michael Levine

Michele Markstein

Bioinformatics methods have identified enhancers that mediate restricted expression in the Drosophila embryo. However, only a small fraction of the predicted enhancers actually work when tested in vivo. In the present study, co-regulated neurogenic enhancers that are activated by intermediate levels of the Dorsal regulatory gradient are shown to contain several shared sequence motifs. These motifs permitted the identification of new neurogenic enhancers with high precision: five out of seven predicted enhancers direct restricted expression within ventral regions of the neurogenic ectoderm. Mutations in some of the shared motifs disrupt enhancer function, and evidence is presented that the Twist and …


The Rpos Sigma Factor In The Dissimilatory Fe(Iii)-Reducing Bacterium Geobacter Sulfurreducens, Lorrie Adams, Susan Childers, Cinthia Núñez, Derek Lovley May 2004

The Rpos Sigma Factor In The Dissimilatory Fe(Iii)-Reducing Bacterium Geobacter Sulfurreducens, Lorrie Adams, Susan Childers, Cinthia Núñez, Derek Lovley

Derek Lovley

Geobacter sulfurreducens RpoS sigma factor was shown to contribute to survival in stationary phase and upon oxygen exposure. Furthermore, a mutation in rpoS decreased the rate of reduction of insoluble Fe(III) but not of soluble forms of iron. This study suggests that RpoS plays a role in regulating metabolism of Geobacter under suboptimal conditions in subsurface environments.


Isolation, Characterization, And U(Vi)-Reducing Potential Of A Facultatively Anaerobic, Acid-Resistant Bacterium From Low-Ph, Nitrate- And U(Vi) Contaminated Subsurface Sediment And Description Of Salmonella Subterranea Sp.Nov., Evgenya S. Shelobolina, Sara A. Sullivan, Kathleen R. O'Neil, Kelly P. Nevin, Derek Lovley May 2004

Isolation, Characterization, And U(Vi)-Reducing Potential Of A Facultatively Anaerobic, Acid-Resistant Bacterium From Low-Ph, Nitrate- And U(Vi) Contaminated Subsurface Sediment And Description Of Salmonella Subterranea Sp.Nov., Evgenya S. Shelobolina, Sara A. Sullivan, Kathleen R. O'Neil, Kelly P. Nevin, Derek Lovley

Kelly Nevin

A facultatively anaerobic, acid-resistant bacterium, designated strain FRCl, was isolated from a low-pH, nitrate- and U(VI)-contaminated subsurface sediment at site FW-024 at the Natural and Accelerated Bioremediation Research Field Research Center in Oak Ridge, Tenn. Strain FRCl was enriched at pH 4.5 in minimal medium with nitrate as the electron acceptor, hydrogen as the electron donor, and acetate as the carbon source. Clones with 16S ribosomal DNA (rDNA) sequences identical to the sequence of strain FRCl were also detected in a U(VI)-reducing enrichment culture derived from the same sediment. Cells of strain FRCl were gram-negative motile regular rods 2.0 to …


Expression Of Haper1 And Habmal1 In Syrian Hamsters: Heterogeneity Of Transcripts And Oscillations In The Periphery, Eric L. Bittman, Yanhong Tong, Hongnian Guo, Judy Mckinley Brewer, Alexamder S. Bois Apr 2004

Expression Of Haper1 And Habmal1 In Syrian Hamsters: Heterogeneity Of Transcripts And Oscillations In The Periphery, Eric L. Bittman, Yanhong Tong, Hongnian Guo, Judy Mckinley Brewer, Alexamder S. Bois

Eric L. Bittman

The molecular biology of circadian rhythms has been extensively studied in mice, and the widespread expression of canonical circadian clock genes in peripheral organs is well established in this species. In contrast, much less information about the peripheral expression of haPer1, haPer2, and haBmal1 is available in Syrian hamsters despite the fact that this species is widely used for studies of circadian organization and photoperiodic responses. Furthermore, examination of oscillating expression of these genes in mouse testis has generated discrepant results, and little is known about gonadal expression of haPer1 and haBmal1 or their environmental control. To address these questions, …


Maca, A Diheme C-Type Cytochrome Involved In Fe(Iii) Reduction By Geobacter Sulfurreducens, Jessica E. Butler, Franz Kaufmann, Cinthia Núñez, Maddalena V. Coppi, Derek Lovley Feb 2004

Maca, A Diheme C-Type Cytochrome Involved In Fe(Iii) Reduction By Geobacter Sulfurreducens, Jessica E. Butler, Franz Kaufmann, Cinthia Núñez, Maddalena V. Coppi, Derek Lovley

Derek Lovley

A 36-kDa diheme c-type cytochrome abundant in Fe(III)-respiring Geobacter sulfurreducens, designated MacA, was more highly expressed during growth with Fe(III) as the electron acceptor than with fumarate. Although MacA has homology to proteins with in vitro peroxidase activity, deletion of macA had no impact on response to oxidative stress. However, the capacity for Fe(III) reduction was greatly diminished, indicating that MacA, which is predicted to be localized in the periplasm, is a key intermediate in electron transfer to Fe(III).


Isolation, Characterization, And U(Vi)-Reducing Potential Of A Facultatively Anaerobic, Acid-Resistant Bacterium From Low-Ph, Nitrate- And U(Vi) Contaminated Subsurface Sediment And Description Of Salmonella Subterranea Sp.Nov., Evgenya S. Shelobolina, Sara A. Sullivan, Kathleen R. O'Neil, Kelly P. Nevin, Derek Lovley Feb 2004

Isolation, Characterization, And U(Vi)-Reducing Potential Of A Facultatively Anaerobic, Acid-Resistant Bacterium From Low-Ph, Nitrate- And U(Vi) Contaminated Subsurface Sediment And Description Of Salmonella Subterranea Sp.Nov., Evgenya S. Shelobolina, Sara A. Sullivan, Kathleen R. O'Neil, Kelly P. Nevin, Derek Lovley

Derek Lovley

A facultatively anaerobic, acid-resistant bacterium, designated strain FRCl, was isolated from a low-pH, nitrate- and U(VI)-contaminated subsurface sediment at site FW-024 at the Natural and Accelerated Bioremediation Research Field Research Center in Oak Ridge, Tenn. Strain FRCl was enriched at pH 4.5 in minimal medium with nitrate as the electron acceptor, hydrogen as the electron donor, and acetate as the carbon source. Clones with 16S ribosomal DNA (rDNA) sequences identical to the sequence of strain FRCl were also detected in a U(VI)-reducing enrichment culture derived from the same sediment. Cells of strain FRCl were gram-negative motile regular rods 2.0 to …


Vanadium Respiration By Geobacter Metallireducens: Novel Strategy For In Situ Removal Of Vanadium From Groundwater, Irene Ortiz-Bernad, Robert T. Anderson, Helen A. Vrionis, Derek Lovley Feb 2004

Vanadium Respiration By Geobacter Metallireducens: Novel Strategy For In Situ Removal Of Vanadium From Groundwater, Irene Ortiz-Bernad, Robert T. Anderson, Helen A. Vrionis, Derek Lovley

Derek Lovley

Vanadium can be an important contaminant in groundwaters impacted by mining activities. In order to determine if microorganisms of the Geobacteraceae, the predominant dissimilatory metal reducers in many subsurface environments, were capable of reducing vanadium(V), Geobacter metallireducens was inoculated into a medium in which acetate was the electron donor and vanadium(V) was the sole electron acceptor. Reduction of vanadium(V) resulted in the production of vanadium(IV), which subsequently precipitated. Reduction of vanadium(V) was associated with cell growth with a generation time of 15 h. No vanadium(V) was reduced and no precipitate was formed in heat-killed or abiotic controls. Acetate was the …


Identification Of An Uptake Hydrogenase Required For Hydrogen-Dependant Reduction Of Fe(Iii) And Other Electron Acceptors By Geobacter Sulfurreducens, Maddalena V. Coppi, Regina A. O'Neil, Derek Lovley Feb 2004

Identification Of An Uptake Hydrogenase Required For Hydrogen-Dependant Reduction Of Fe(Iii) And Other Electron Acceptors By Geobacter Sulfurreducens, Maddalena V. Coppi, Regina A. O'Neil, Derek Lovley

Derek Lovley

Geobacter sulfurreducens, a representative of the family Geobacteraceae that predominates in Fe(III)-reducing subsurface environments, can grow by coupling the oxidation of hydrogen to the reduction of a variety of electron acceptors, including Fe(III), fumarate, and quinones. An examination of the G. sulfurreducens genome revealed two operons, hya and hyb, which appeared to encode periplasmically oriented respiratory uptake hydrogenases. In order to assess the roles of these two enzymes in hydrogen-dependent growth, Hya- and Hyb-deficient mutants were generated by gene replacement. Hyb was found to be required for hydrogen-dependent reduction of Fe(III), anthraquinone-2,6-disulfonate, and fumarate by resting cell suspensions and to …


Cranberry Irrigation Management, Hilary A. Sandler, Carolyn J. Demoranville, Bruce Lampinen Feb 2004

Cranberry Irrigation Management, Hilary A. Sandler, Carolyn J. Demoranville, Bruce Lampinen

Cranberry Station Fact Sheets

No abstract provided.


Preferential Reduction Of Fe(Iii) Over Fumarate By Geobacter Sulfurreducens, Abraham Esteve-Núñez, Cinthia Núñez, Derek Lovley Jan 2004

Preferential Reduction Of Fe(Iii) Over Fumarate By Geobacter Sulfurreducens, Abraham Esteve-Núñez, Cinthia Núñez, Derek Lovley

Derek Lovley

The presence of Fe(III), but not that of Fe(II), resulted in ca. 20-fold-lower levels of mRNA for fumarate reductase, inhibiting fumarate reduction and favoring utilization of fumarate as an electron donor in chemostat cultures of Geobacter sulfurreducens, despite the fact that growth yield with fumarate was 3-fold higher than with Fe(III).


Immunity Regulatory Dnas Share Common Organizational Features In Drosophila, Kate Senger, Grant W. Armstrong, William J. Rowell, Jennifer M. Kwan, Michele Markstein, Michael Levine Jan 2004

Immunity Regulatory Dnas Share Common Organizational Features In Drosophila, Kate Senger, Grant W. Armstrong, William J. Rowell, Jennifer M. Kwan, Michele Markstein, Michael Levine

Michele Markstein

Infection results in the rapid activation of immunity genes in the Drosophila fat body. Two classes of transcription factors have been implicated in this process: the REL-containing proteins, Dorsal, Dif, and Relish, and the GATA factor Serpent. Here we present evidence that REL-GATA synergy plays a pervasive role in the immune response. SELEX assays identified consensus binding sites that permitted the characterization of several immunity regulatory DNAs. The distribution of REL and GATA sites within these DNAs suggests that most or all fat-specific immunity genes contain a common organization of regulatory elements: closely linked REL and GATA binding sites positioned …


Disorganization Of Cortical Microtubules Stimulates Tangential Expansion And Reduces The Uniformity Of Cellulose Microfibril Alignment Among Cells In The Root Of Arabidopsis, Ti Baskin, Gts Beemster, Je Judy-March, F Marga Jan 2004

Disorganization Of Cortical Microtubules Stimulates Tangential Expansion And Reduces The Uniformity Of Cellulose Microfibril Alignment Among Cells In The Root Of Arabidopsis, Ti Baskin, Gts Beemster, Je Judy-March, F Marga

Biology Department Faculty Publication Series

No abstract provided.


Photoperiodic Regulation Of Androgen Receptor And Steroid Receptor Coactivator-1 In Siberian Hamster Brain, Mj Tetel, Tc Ungar, B Hassan, El Bittman Jan 2004

Photoperiodic Regulation Of Androgen Receptor And Steroid Receptor Coactivator-1 In Siberian Hamster Brain, Mj Tetel, Tc Ungar, B Hassan, El Bittman

Biology Department Faculty Publication Series

No abstract provided.


In Vivo Dose-Response Of Insects To Hz-2v Infection, John Burand, Christopher Rallis Jan 2004

In Vivo Dose-Response Of Insects To Hz-2v Infection, John Burand, Christopher Rallis

Microbiology Department Faculty Publication Series

Background Hz-2V infection of female Helicoverpa zea moths is manifested as insects that are either sterile "agonadal" individuals with malformed reproductive tissues or fertile asymptomatic carriers which are capable of transmitting virus on to their progeny. Virus infected progeny arising from eggs laid by asymptomatic carrier females may themselves be either sterile agonadals or asymptomatic carriers. Results By injecting virus into female moths, a correlation was established between virus doses administered to the females and the levels of resulting asymptomatic and sterile progeny. Conclusions The results of these experiments indicate that high virus doses produced a higher level of agonadal …


Phosphorus For Bearing Cranberries In North America, Teryl Roper, Joan Davenport, Carolyn J. Demoranville, Sebastien Marchand, Art Poole, Kim Patten Jan 2004

Phosphorus For Bearing Cranberries In North America, Teryl Roper, Joan Davenport, Carolyn J. Demoranville, Sebastien Marchand, Art Poole, Kim Patten

Cranberry Station Fact Sheets

No abstract provided.


Expression Of Haper1 And Habmal1 In Syrian Hamsters: Heterogeneity Of Transcripts And Oscillations In The Periphery, Yh Tong, Hn Guo, Jm Brewer, H Lee, Mn Lehman, El Bittman Jan 2004

Expression Of Haper1 And Habmal1 In Syrian Hamsters: Heterogeneity Of Transcripts And Oscillations In The Periphery, Yh Tong, Hn Guo, Jm Brewer, H Lee, Mn Lehman, El Bittman

Biology Department Faculty Publication Series

No abstract provided.


Food Hardness And Feeding Behavior In Old World Fruit Bats (Pteropodidae), Elizabeth R. Dumont, Reilly O'Neal Jan 2004

Food Hardness And Feeding Behavior In Old World Fruit Bats (Pteropodidae), Elizabeth R. Dumont, Reilly O'Neal

Biology Department Faculty Publication Series

Old World fruit bats (family Pteropodidae) are common throughout the Paleotropics, where they play an important ecological role as seed dispersers and pollinators. Although many regions host diverse assemblages of fruit bats, mechanisms of resource partitioning are only beginning to be documented. This study investigates the potential role of feeding behavior in patterns of resource use within a sympatric assemblage of pteropodids from Papua New Guinea. Individuals of Syconycteris australis, Dobsonia minor, Pteropus conspicillatus, Nyctimene albiventer, and Paranyctimene raptor were videotaped during feeding experiments designed to elicit shifts in feeding (biting) behavior by varying fruit hardness. Although significant variation …