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In Situ Expression Of Nigd In Geobacteraceae In Subsurface Sediments, Dawn E. Holmes, Kelly P. Nevin, Derek Lovley
In Situ Expression Of Nigd In Geobacteraceae In Subsurface Sediments, Dawn E. Holmes, Kelly P. Nevin, Derek Lovley
Kelly Nevin
In order to determine whether the metabolic state of Geobacteraceae involved in bioremediation of subsurface sediments might be inferred from levels of mRNA for key genes, in situ expression of nifD, a highly conserved gene involved in nitrogen fixation, was investigated. When Geobacter sulfurreducens was grown without a source of fixed nitrogen in chemostats with acetate provided as the limiting electron donor and Fe(III) as the electron acceptor, levels of nifD transcripts were 4 to 5 orders of magnitude higher than in chemostat cultures provided with ammonium. In contrast, the number of transcripts of recA and the 16S rRNA gene …
Isolation, Characterization, And U(Vi)-Reducing Potential Of A Facultatively Anaerobic, Acid-Resistant Bacterium From Low-Ph, Nitrate- And U(Vi) Contaminated Subsurface Sediment And Description Of Salmonella Subterranea Sp.Nov., Evgenya S. Shelobolina, Sara A. Sullivan, Kathleen R. O'Neil, Kelly P. Nevin, Derek Lovley
Isolation, Characterization, And U(Vi)-Reducing Potential Of A Facultatively Anaerobic, Acid-Resistant Bacterium From Low-Ph, Nitrate- And U(Vi) Contaminated Subsurface Sediment And Description Of Salmonella Subterranea Sp.Nov., Evgenya S. Shelobolina, Sara A. Sullivan, Kathleen R. O'Neil, Kelly P. Nevin, Derek Lovley
Kelly Nevin
A facultatively anaerobic, acid-resistant bacterium, designated strain FRCl, was isolated from a low-pH, nitrate- and U(VI)-contaminated subsurface sediment at site FW-024 at the Natural and Accelerated Bioremediation Research Field Research Center in Oak Ridge, Tenn. Strain FRCl was enriched at pH 4.5 in minimal medium with nitrate as the electron acceptor, hydrogen as the electron donor, and acetate as the carbon source. Clones with 16S ribosomal DNA (rDNA) sequences identical to the sequence of strain FRCl were also detected in a U(VI)-reducing enrichment culture derived from the same sediment. Cells of strain FRCl were gram-negative motile regular rods 2.0 to …