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Infogest Inter-Laboratory Recommendations For Assaying Gastric And Pancreatic Lipases Activities Prior To In Vitro Digestion Studies, Myriam M.L. Grundy, Evan Abrahamse, Annette Almgren, David J. Mcclements, Marie Alminger, Ana Andres, Renata M.C. Ariëns, Shanna Bastiaan-Net, Claire Bourlieu-Lacanal, André Brodkorb
Infogest Inter-Laboratory Recommendations For Assaying Gastric And Pancreatic Lipases Activities Prior To In Vitro Digestion Studies, Myriam M.L. Grundy, Evan Abrahamse, Annette Almgren, David J. Mcclements, Marie Alminger, Ana Andres, Renata M.C. Ariëns, Shanna Bastiaan-Net, Claire Bourlieu-Lacanal, André Brodkorb
Food Science Department Faculty Publication Series
In vitro digestion studies often use animal digestive enzyme extracts as substitutes of human gastric and pancreatic secretions. Pancreatin from porcine origin is thus commonly used to provide relevant pancreatic enzymes such as proteases, amylase and lipase. Rabbit gastric extracts (RGE) have been recently introduced to provide gastric lipase in addition to pepsin. Before preparing simulated gastric and pancreatic extracts with targeted enzyme activities as described in in vitro digestion protocols, it is important to determine the activities of enzyme preparations using validated methods. The purpose of this inter-laboratory study within the INFOGEST network was to test the repeatability and …