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Overexpression Of An Arabidopsis Formin Stimulates Supernumerary Actin Cable Formation From Pollen Tube Cell Membrane, Alice Cheung, H. M. Wu
Overexpression Of An Arabidopsis Formin Stimulates Supernumerary Actin Cable Formation From Pollen Tube Cell Membrane, Alice Cheung, H. M. Wu
Alice Cheung
Formins, actin-nucleating proteins that stimulate the de novo polymerization of actin filaments, are important for diverse cellular and developmental processes, especially those dependent on polarity establishment. A subset of plant formins, referred to as group I, is distinct from formins from other species in having evolved a unique N-terminal structure with a signal peptide, a Pro-rich, potentially glycosylated extracellular domain, and a transmembrane domain. We show here that overexpression of the Arabidopsis formin AFH1 in pollen tubes induces the formation of arrays of actin cables that project into the cytoplasm from the cell membrane and that its N-terminal structure targets …