Life Sciences Commons^™

The Effect Of Calcium Ions On Mechanosensation And Neuronal Activity In Proprioceptive Neurons, Devan E. Atkins, Kimberly L. Bosh, Grace W. Breakfield, Sydney E. Daniels, Makayla J. Devore, Hailey E. Fite, Landys Z. Guo, Danielle K. J. Henry, Alana K. Kaffenberger, Katherine S. Manning, Tatum E. Mowery, Cecilia L. Pankau, Nyla Parker, Malina E. Serrano, Yamaan Shakhashiro, Hannah N. Tanner, Ruth. A. Ward, Aubrey H. Wehry, Robin L. Cooper

Biology Faculty Publications

Proprioception of all animals is important in being able to have coordinated locomotion. Stretch activated ion channels (SACs) transduce the mechanical force into electrical signals in the proprioceptive sensory endings. The types of SACs vary among sensory neurons in animals as defined by pharmacological, physiological and molecular identification. The chordotonal organs within insects and crustaceans offer a unique ability to investigate proprioceptive function. The effects of the extracellular environment on neuronal activity, as well as the function of associated SACs are easily accessible and viable in minimal saline for ease in experimentation. The effect of extracellular [Ca²⁺] on …

Manganese-Enhanced Magnetic Resonance Imaging: Overview And Central Nervous System Applications With A Focus On Neurodegeneration, Ryan A. Cloyd, Shon A. Koren, Jose F. Abisambra

Physiology Faculty Publications

Manganese-enhanced magnetic resonance imaging (MEMRI) rose to prominence in the 1990s as a sensitive approach to high contrast imaging. Following the discovery of manganese conductance through calcium-permeable channels, MEMRI applications expanded to include functional imaging in the central nervous system (CNS) and other body systems. MEMRI has since been employed in the investigation of physiology in many animal models and in humans. Here, we review historical perspectives that follow the evolution of applied MRI research into MEMRI with particular focus on its potential toxicity. Furthermore, we discuss the more current in vivo investigative uses of MEMRI in CNS investigations and …

Identification Of Changes In Neuronal Function As A Consequence Of Aging And Tauopathic Neurodegeneration Using A Novel And Sensitive Magnetic Resonance Imaging Approach, Sarah N. Fontaine, Alexandria Ingram, Ryan A. Cloyd, Shelby E. Meier, Emily Miller, Danielle N. Lyons, Grant K. Nation, Elizabeth Mechas, Blaine Weiss, Chiara Lanzillotta, Fabio Di Domenico, Frederick A. Schmitt, David K. Powell, Moriel H. Vandsburger, Jose Francisco Abisambra

Sanders-Brown Center on Aging Faculty Publications

Tauopathies, the most common of which is Alzheimer’s disease (AD), constitute the most crippling neurodegenerative threat to our aging population. Tauopathic patients have significant cognitive decline accompanied by irreversible and severe brain atrophy, and it is thought that neuronal dysfunction begins years before diagnosis. Our current understanding of tauopathies has yielded promising therapeutic interventions but have all failed in clinical trials. This is partly due to the inability to identify and intervene in an effective therapeutic window early in the disease process. A major challenge that contributes to the definition of an early therapeutic window is limited technologies. To address …

Development And Deployment Of A Bioreactor For The Removal Of Sulfate And Manganese From Circumneutral Coal Mine Drainage, C. B. Mastin, J. D. Edwards, Christopher D. Barton, Anastasios D. Karathanasis, Carmen T. Agouridis, Richard C. Warner

Forestry and Natural Resources Faculty Publications

Surface mining, in the form of contour mining and mountain-top removal, is a common means for retrieving coal in the Appalachian Coal Belt region of Kentucky. Overburden or excess spoil generated by these two methods is placed in valley fills. Traditionally Constructed fills have been shown to adversely impact headwater ecosystems via stream burial and through alterations to the hydrology, sediment supply, water quality and biological composition of downstream reaches. Mine drainages emanating from the toe of valley fills often contain elevated levels of total dissolved solids and heavy metals. Drainage chemistry from Guy Cove, a valley fill located in …

Manganese Distribution Across The Blood-Brain Barrier. Iv. Evidence For Brain Influx Through Store-Operated Calcium Channels, Janelle S. Crossgrove, Robert A. Yokel

Pharmaceutical Sciences Faculty Publications

Manganese (Mn) is a required co-factor for many ubiquitous enzymes; however, chronic Mn overexposure can cause manganism, a parkinsonian-like syndrome. Previous studies showed Mn influx into brain is carrier-mediated, though the putative carrier(s) were not established. Studies conducted with cultured bovine brain microvascular endothelial cells (bBMECs), which comprise the blood–brain barrier, revealed ⁵⁴Mn (II) uptake positively correlated with pH, was temperature-dependent, and was sodium- and energy-independent. Brain ⁵⁴Mn uptake correlated inversely with calcium (Ca) concentration, but ⁴⁵Ca uptake was unaltered by high Mn concentration. Lanthanum (La), a non-selective inhibitor of several Ca channel types, as well as …

Manganese Distribution Across The Blood-Brain Barrier. I. Evidence For Carrier-Mediated Influx Of Managanese Citrate As Well As Manganese And Manganese Transferrin, Janelle S. Crossgrove, David D. Allen, Bonny L. Bukaveckas, Susan S. Rhineheimer, Robert A. Yokel

Pharmaceutical Sciences Faculty Publications

Manganese (Mn) is an essential element and a neurotoxicant. Regulation of Mn movement across the blood–brain barrier (BBB) contributes to whether the brain Mn concentration is functional or toxic. In plasma, Mn associates with water, small molecular weight ligands and proteins. Mn speciation may influence the kinetics of its movement through the BBB. In the present work, the brain influx rates of ⁵⁴Mn²⁺, ⁵⁴Mn citrate and ⁵⁴Mn transferrin (⁵⁴Mn Tf) were determined using the in situ brain perfusion technique. The influx rates were compared to their predicted diffusion rates, which were determined from …

Effect Of Banded Fertilizer On Manganese Toxicity Of Burley Tobacco, J. L. Sims, Kenneth L. Wells, E. C. Greer

Agronomy Notes

Relatively large amounts (1500 to 3000 lbs per acre) of inorganic fertilizers are commonly broadcast preplant in the production of burley tobacco. Such high rates of fertilizer increase both the salt content of the soil solution and soil acidity, often causing reduced stands, manganese toxicity, plant molybdenum and calcium deficiencies, delayed growth and maturity, and reduced yield and quality of cured leaf. Manganese toxicity alone is thought to cost growers 30-40 million dollars each year from yield loss and the costs associated with growing extra acreage the following year to make up their quota¥ Improved fertilizer management systems are needed …

Effect Of Fertilizer Rates And Placement Practices On Yield Of Burley Tobacco, Kenneth L. Wells, G. D. Cantrill, J. L. Sims

Agronomy Notes

Management of fertilizer practices in production of burley tobacco is very important in control of manganese (Mn) toxicity of the crop. In addition to the use of agricultural limestone, the use of fertilizers in the appropriate amounts, the appropriate kinds, and in the appropriate manner can strongly influence acidity in the rooting zone during the growth of burley. Studies of these effects have made up a major thrust of the University of Kentucky's research programs on fertility of burley, and have largely been conducted by J.L. Sims and his students during the past 15 years.

Manganese Fertilization Of Soybeans, Lloyd W. Murdock, Harold F. Miller, Doyle Peaslee, Wilbur Frye

Agronomy Notes

Only a small quantity of manganese is essential for plant growth however under certain soil conditions even this is not available to the plant. Depending on severity, manganese deficiency will retard plant growth and may drastically decrease yields. A deficiency of manganese will affect the photosynthesis system of the plant. The visual symptoms of a manganese deficiency on soybeans is interveinal chlorosis of the leaves. The veins remains green until the chlorosis nears the white stage then they also lose their color.

Plant Beds, Jones H. Smiley, Ira E. Massie

Agronomy Notes

Due to the occurrence of blue mold and manganese toxicity in some of the plant beds this last spring, it is probably advisable for all growers to select a new site for the 1964 crop.

Blue mold is a fungus disease which sometimes causes severe damage to tobacco seedings just prior to setting time. The fungus will produce heavy-walled spores on the dying or dead leaves. These spores are capable of remaining in the soil at least a year and attacking the next crop of plants if the same site is used again for tobacco plants .

Determine Liming Needs On Tobacco Land This Fall, Jones H. Smiley, Harold F. Miller

Agronomy Notes

There have been far too many reports of manganese toxicity in tobacco this season for a condition which is so easily corrected.

Manganese is one of the minor elements necessary for plant growth, but it is needed in very small amounts. When large amounts are taken up by a tobacco plant, light green or yellowish areas appear between the larger veins of the leaf. In severe cases the plant may be stunted or even die.

Agronomy Notes, No. 1, S. H. Phillips, Harold F. Miller, Verne C. Finkner

Agronomy Notes

This is the first issue of "Agronomy Notes." The Agronomy Department, University of Kentucky, expects to use this publication to inform county agents, other agricultural workers, and leaders on current progress in soils & crops work. The Kentucky Experiment Station will be an important source of information. Information may be drawn from other research. Field trials and observations that show useful information may be reported. The emphasis will be on short timely topics.